nucleic acid based diagnosis in clinical materials

... deciphering the origin of the destabilization and hence, diminution of the melting temperature (Tm) in the former Base stacking in the vicinity of A C mismatch in PNAÆDNA and DNA duplexes Intra ... C2¢ endo domain Discussion Base pair mismatch in duplex DNA contributes an element of instability resulting in lowering of the melting temperature and increase in free energy Surprisingly, the ... hydrogen bond in PNAÆDNA than in DNA duplex As expected, these bring forth significant variableness in the water interactions surrounding the mismatch Water interaction in the vicinity of A C mismatch...

Ngày tải lên: 07/03/2014, 21:20

Ngày tải lên: 14/11/2014, 10:13

... complete cartilaginous rings causing tracheal stenosis CLINICAL PRESENTATION OF VASCULAR RINGS AND SLINGS Most infants present with symptoms in early infancy Superimposed viral infection with edema ... using the text to facilitate dialogue Samir S Shah Stephen Ludwig CHAPTER WHEEZING DUSTIN R HAFERBECKER DEFINITION OF THE COMPLAINT Noisy breathing in infants is a common presenting complaint ... (inferior vena cava, azygous vein, or portal vein) Drainage into systemic veins produces a left to right shunt In this case, the vessels appeared to drain into the azygous and hemiazygous veins...

Ngày tải lên: 13/03/2016, 14:30

... element binding protein: cellular nucleic acid binding protein Virus Res 58, 73–82 Flink IL & Morkin E (1995) Alternatively processed isoforms of cellular nucleic acid- binding protein interact ... during 15 with different kinase inhibitors at 30 °C and then used to analyse in vitro recombinant CNBP phosphorylation in kinase buffer containing 100 lm ATP and lCi [32P]ATP[cP] at 30 °C for Kinase ... cellular nucleic acid binding protein cDNA Gene 218, 95–101 19 van Heumen WR, Claxton C & Pickles JO (1997) Sequence and tissue distribution of chicken cellular nucleic acid binding protein cDNA...

Ngày tải lên: 16/03/2014, 12:20

... covalently link them To facilitate purification, both dlFG and a single-chain dimer, sc-dlFG, contain an N-terminal six-histidine nickelaffinity tag Using intrinsic fluorescence of Trp residues, extrinsic ... conserved arginine-rich RNA binding motif in cowpea chlorotic mottle virus capsid protein results in virion structural alterations and RNA packaging constraints J Virol 79, 3277–3288 Dertinger D, ... protein and RNA has been investigated [5,9,23,24], and amino acid nucleotide interactions contributing to its stability have been characterized [13,25], but the role of protein–protein and protein–RNA...

Ngày tải lên: 30/03/2014, 11:20

... the ATP-binding domain of protein kinases revealed that the sequence from amino acid residue 431 to 457 of iPLA2fl exhibits similarity to the region of protein kinases involved in ATP binding (Fig ... results indicate that iPLA.2~ contains eonsensns phosphorylation sites for calcium/calmodulin-dependent protein kinase II, protein kinase A, protein kinase C, protein kinase G, and casein kinase ... defined amino acid sequence motif (80); thus, it is relatively difficult to identify cahnodulin-binding domains from sequence analyses The amino acid sequences of a number of cahnodulin-binding...

Ngày tải lên: 05/05/2014, 16:43

... treatment of catheter-associated urinary tract infection in adults: 2009 International Clinical Practice Guidelines from the Infectious Diseases Society of America Clin Infect Dis 2010;50(5):625-63 ... biosensors remains in translating the technologies from detection in laboratory solutions to real-world clinical samples, such as blood, serum and urine The complex sample matrices of clinical samples ... targetbinding affinity, making label-free detection in clinical samples a possibility [48,49] Labeled biosensors Labeled assays are the most common and robust method of biosensing Classically, in...

Ngày tải lên: 13/05/2014, 13:59

... providing new concepts in materials science and engineering.17 Biomineralisation studies the mineral formation processes in living entities It encompasses the whole animal kingdom, from single-cell ... specialists interested in specific topics of this field At the beginning of each chapter, the introduction provides insight on the corresponding developed topic In some cases, the different introductions ... relationship in different biominerals function) bear in common the crystallisation of inorganic phases within an organic matrix, which can be considered as a bonding agent arranging the crystals in certain...

Ngày tải lên: 04/06/2014, 15:18

... cause a major change during clinical practise Therefore an interdisciplinary dialogue and planning is indispensable for this procedure Furthermore lymph node harvesting in fresh tissue is much ... H&E staining and OSNA None of the LN was HE+/OSNA- and of the 63 LN were HE-/OSNA+, resulting in upstaging of two patients [25] The aspect of upstaging was not the main focus of this investigation ... 3-4 LN within 30-40 minutes and 12 LN within hours For the reason that increasing numbers of investigated LN correlate with a more accurate tumour staging, high throughput methods are indispensable...

Ngày tải lên: 18/06/2014, 16:20

... frequency One of the most interesting findings in this study is the wide variability in distribution of sexual risk in the population The number of individuals engaging in risk behaviors, sexual ... and partners exposed among 30 patients engaging in sexual risk followed longitudinally in clinical care Thirty patients engaging in unprotected sex during a cumulative total of 12 months: Cumulative ... mutations in chronically HIV-infected, treatment naïve participants in the cpcra first study: implications for routine resistance screening prior to initiating antiretroviral therapy Clin Infect...

Ngày tải lên: 20/06/2014, 08:20

... correct for interfractional setup errors For the study presented in this paper, we installed the in- line imaging equipment onto the linear accelerator of the PRIMATOM system Imaging system and ... Dealing with rotational errors In this work, we implemented rigid matching (detecting translational and rotational errors) and correction of tranlational errors only into the clinical workflow In ... implies a reduction of margins Optimal would be the elimination of interfraction and intrafraction organ motion with the objective of minimizing the margins of the planning target volume The cone...

Ngày tải lên: 09/08/2014, 10:21

... proinflammatory cytokine signaling, including suppressor of cytokine signaling (SOCS1) and the Tyro-3, Axl, and Mer (TAM) receptors, have been shown to associate with, and regulate signaling ... therapy based on the targeting of subsets of nucleic acidassociated autoantigens These studies are of particular importance given that IFN-I and TLR inhibitors are already being tested in SLE in early-phase ... human clinical trials Our studies provide a crucial link between the IFN-I system and TLR signaling in vivo and suggest that IFN-I is upstream of TLRs in the loss of B-cell tolerance to nucleic acid- associated...

Ngày tải lên: 09/08/2014, 14:22

... http://www.implementationscience.com/content/6/1/62 of interest Applying GRADE begins with formulating appropriate clinical questions using the PICO or another structured format [16] This step leads to focussed clinical questions pertaining to ... specifying a clinical setting based on history, clinical presentation, and results of index tests alone (i.e., without performing a reference test, the OFC) In detail, we applied the following process ... developing recommendations about diagnostic tests with guideline panels Our case study is based on using the GRADE approach for a guideline with the World Allergy Organization (WAO) [5] in the clinical...

Ngày tải lên: 10/08/2014, 10:23

... domains The AR is characterized by a modular structure consisting of four functional domains: an N-terminal domain (NTD), a DNA-binding domain (DBD), a hinge region, and a ligand-binding domain ... in the AR and it is the most conserved region within the nuclear receptor family This region has nine cysteine residues, eight of which are involved in forming two zinc fingers, and a C-terminal ... manuscript DMS was involved in collating information regarding the case and getting informed consent from our patient ADC was involved in the review of literature and revising the manuscript...

Ngày tải lên: 10/08/2014, 23:20

... participants included in this trial [38] It is common in trials assessing the effectiveness of interventions aimed at increasing the uptake of evidence in clinical practice to only include outcome ... 54 55 56 57 58 59 60 61 62 King DW, Hazelwood M: Quantifying Gains in Data Quality for Sampling Plans Used in Clinical Trial Monitoring Drug Inf J 2003, 37:135-141 King DW, Lashley R: A quantifiable ... criteria reflect the clinical scope of the acute LBP CPG [3] 1) Radicular leg pain is present We define this as leg pain that is described as shooting, lancinating, or electric in quality, extends...

Ngày tải lên: 11/08/2014, 05:22

... substitutions occurring in distant species or motif searching in a single species Some other methods train a Markov model to capture nucleotide dependency information of known binding sites in order to ... iTriplet in predicting transcription factor binding sites in E Coli These binding sites are experimentally validated and documented in the RegulonDB database [20] The test was conducted using the ... iTriplet with the predicted motifs being indicated in Figure According to a NMR structural study of the U/GU-rich binding protein CstF-64 [28], we believe the binding site should not be longer than...

Ngày tải lên: 12/08/2014, 17:20

... anion, resulting in an expected increase in SID as Na+ is maintained but Cl– falls; the increased SID drives proton consumption and produces an increased pH Importantly, only changes in strong ions ... patients requiring ICU care, SIG > was independently linked with mortality in patients evidencing metabolic acidosis [48] Based on these studies, longitudinal assessments of changes in the SIG as ... remains an important prognostic indicator J Trauma 2002, 53:838-842 Scheingraber S, Rehm M, Sehmisch C, Finsterer U: Rapid saline infusion produces hyperchloremic acidosis in patients undergoing...

Ngày tải lên: 12/08/2014, 20:20

... lactic acidosis: a prospective controlled clinical study Ann Intern Med 1990, 112:492-498 Scheingraber S, Rehm M, Sehmisch C, Finsterer U: Rapid saline infusion produces hyperchloremic acidosis in ... alkalinizing, much as Hartmann originally intended [49], it is close to the ideal from an acid base perspective Slight alkalinization is difficult to demonstrate in laboratory and especially in clinical ... respiratory acidosis after large intravascular volume infusion of lactated Ringer’s solution during major spine surgery Anesth Analg 2002, 95:294-298 55 Myburgh JA: Severe head injury In Oh’s Intensive...

Ngày tải lên: 12/08/2014, 20:20

... [51] reported similar findings in large volume resuscitation in major surgery comparing hetastarch prepared in a balanced electrolyte solution or in saline, and similar findings were reported by ... addressed in future study designs Without consistency in reporting acid base methodology, conflicting reports will continue Competing interests The author(s) declare that they have no competing interests ... for the acidosis (Fig 2) In this system, metabolic acidosis is defined as a SBE below mEq/l; lactic acidosis is an acidosis in which lactate accounts for more than 50% of the SBE; in SIG acidosis...

Ngày tải lên: 12/08/2014, 22:22