nuclear magnetic resonance spectroscopy studies of cancer cell metabolism

... one out of three cows resembling PG toxicosis and the present study aimed to investigate the metabolites responsible for PGinduced toxicosis using proton nuclear magnetic resonance (NMR) spectroscopy ... attractive Proton NMR spectroscopy, which in principle enables detection of all hydrogen-containing molecules, has turned into a commonly applied technique for metabolic profiling of biofluids, among ... sampling and no signs of lack of tolerance to the ration were noticed On the sampling day, which was designated as the sampling day, two of the cows consumed the entire amount of offered concentrate...

Ngày tải lên: 12/08/2014, 18:22

Ngày tải lên: 16/04/2015, 08:57

Ngày tải lên: 12/08/2016, 14:14

... effect Only on closer examination of the full metabolic requirements of a cancer cell was the advantage of PKM2 expression revealed A cancer cell, like any normal cell, must obtain the building ... Senescence • Cell death ROS level • Proliferation • Cell survival Cancer cell • Metabolism • Protein translation Antioxidants Nature effect of Figure | relationship between the levels of rOS and cancer ... importance of nADPH in cancer cell metabolism has prompted proposals of clinical intervention by inhibiting nADPH production Attenuation of the PPP would theoretically dampen nADPH production in cancer...

Ngày tải lên: 15/02/2014, 04:20

... agegroups of rats, young and senescent, in which selected metabolites were quantified by means of proton magnetic resonance spectroscopy The ultimate goal of the present study was the identification of ... quantitative in vitro 1H NMR spectroscopic studies J Neurochem 1995, 65:1192-1198 Petroff OA, Ogino T, Alger JR: High-resolution proton magnetic resonance spectroscopy of rabbit brain regional metabolite ... metabolites of hypoxia and hyperoxia overlaid on cerebral ischemia in young and old rats: a quantitative proton magnetic resonance spectroscopy study Journal of Biomedical Science 2010, 17:14 Page of...

Ngày tải lên: 10/08/2014, 05:21

... per minute µl Micro liter MRS Nuclear magnetic resonance spectroscopy NMR Nuclear magnetic resonance NOE Nuclear Overhauser effect NOESY Nuclear Overhauser enhanced spectroscopy RMSD Root-mean-square ... studies Nuclear magnetic resonance phenomenon was first detected by Bloch and Purcell independently in 1946 (Bloch, 1946; Purcell, 1946) They found that when certain nuclei were placed in a magnetic ... metabolites of different types of brain tumors b) Quantitative studies of major brain metabolites of both human and mouse c) Make it possible to clinical diagnosis of brain tumors by NMR spectra of metabolites...

Ngày tải lên: 10/11/2015, 11:35

... NMR data, Journal of Magnetic Resonance 183(1): 145 151 24 22 Magnetic Resonance Spectroscopy Magnetic Resonance Spectroscopy Cobas, J C & Sardina, F J (2003) Nuclear Magnetic Resonance data processing ... removal of in vivo H MRS signals of 10 Magnetic Resonance Spectroscopy Magnetic Resonance Spectroscopy human brain using HLSVD with a model order of 25 (K = 25) Nevertheless, suppression of unwanted ... quantication of series of biomedical magnetic resonance spectroscopy signals, Journal of Magnetic Resonance 140(1): 120 130 Vanhamme, L., Sundin, T., Van Hecke, P & Van Huffel, S (2001) MR spectroscopy...

Ngày tải lên: 17/03/2014, 02:20

... measurement in vivo of oxygen tension is performed by the use of imaging techniques [18,22,23], most of which are based on magnetic resonance Blood oxygen level-dependent magnetic resonance imaging ... targets of these treatments comprises the endothelial cells One endothelial cell can control the survival of approximately 50–100 tumour cells [93] Therefore, the destruction of a few endothelial cells ... hypoxia on cancer cells and endothelial cells within a tumour Fig Schematic representation of the effects of HIF-1 activation under intermittent hypoxia on cancer cells and endothelial cells within...

Ngày tải lên: 30/03/2014, 04:20

... TFA Salt Stoichiometry Solid-state Nuclear Magnetic Resonance  NMR in solids, like solution-state, relies on the behavior of nuclear spin energy levels in a magnetic field However, the interactions ... HMBC analysis of carvedilol: Structural Analysis: 1H –15N Correlation The 1H-15N longrange HMQC analysis of telithromycin: Determination of Relative Stereochemistry NOE difference spectroscopy ... correlated spectroscopy HMQC heteronuclear multiple quantum coherence 1H-13C covalent bonding, bond HMBC heteronuclear multiple bond correlation 1H-13C NOE difference, NOESY, ROESY nuclear Overhauser...

Ngày tải lên: 27/06/2014, 01:20

... Electromagnetic Spectrum  NMR, MRI  EPR/ESR What is NMR?  NMR is an experiment in which the resonance frequencies of nuclear magnetic systems are investigated  NMR always employs some form of magnetic ... “Principles of Nuclear Magnetism”, Oxford,   1961 R R Ernst, G Bodenhausen, and A Wokaun, “Principles of Nuclear Magnetic Resonance in One and Two Dimensions”, Oxford, 1987 C P Slichter, “Principles of ... saturation of the conduction electron spin resonance in a metal, the nuclear spins would be polarized 1000 times more than normal!!! 16 The Nuclear Overhauser Effect  Dipolar coupling is a direct magnetic...

Ngày tải lên: 27/06/2014, 01:20

... from neuropsychological studies of those who are at genetic risk for schizophrenia have been inconsistent [8,9], partly because these studies often rely on the assumption of elevated genetic risk ... related to people with schizophrenia Neuroimaging studies of high-risk populations have recently begun to investigate a subgroup of relatives of patients with schizophrenia, sometimes referred ... smoothing using a Gaussian kernel of full width at half maximum (FWHM) mm; mean-based intensity normalisation of all volumes by the same factor; high-pass filter cut-off of 100 s; high-pass temporal...

Ngày tải lên: 08/08/2014, 23:21

... duration of more than eight years between the start of symptoms and the diagnosis of AS is reported [3,4] Such a delay is increasingly unwarranted because of the availability of effective treatment Magnetic ... History of inflammatory bowel disease present (%) 15 History of uveitis present (%) 15 History of psoriasis present (%) 24 Family history of ankylosing spondylitis present (%) 37 aForty-five of the ... Cohen's kappa) Results Patients Of the 70 patients that were selected for the study, two patients were excluded (one because of claustrophobia and one because of withdrawal of consent); therefore, complete...

Ngày tải lên: 09/08/2014, 07:20

... Page of Author details Department of Clinical Radiology, University Hospital of Ioannina, Leoforos S Niarchou, 45500, Ioannina, Greece 2Department of Urology, University Hospital of Ioannina, Leoforos ... component of an adenomatoid tumor of the tunica albuginea The presence of abundant collagenproducing fibroblastic cells and a dense network of collagen fibres cause restriction in the diffusion of the ... localization of the mass and helping in characterizing the benign nature of fibrous paratesticular tumor, by using both the conventional and diffusion MRI Confirmation of the diagnostic efficacy of MRI...

Ngày tải lên: 11/08/2014, 00:23

... of a cancer cell clone, aid in overcoming an anti -cancer defence mechanism, or aid in the competitive advantage of a given cell clone Mutations favourable to the growth of a cancer cell may of ... of cancer stem cells? Results Accumulation of mutations favourable to the growth of a cancer What mutations are “favourable” to a cancer cell? Favourable mutations to a cancer cell could include ... size of tumour, because of the much smaller number of stem cells, clonal evolution is predicted to be faster in stem cells compared to non-stem cells What are the mechanisms by which one cancer cell...

Ngày tải lên: 13/08/2014, 16:20

... scatter, the fine detail of the single cell linear patterns is lost There is also a second RCP at the end of the cycle A much more detailed analysis of populations of single cells followed on films ... measuring cell length and diameter This has been done for fifty or more single cells of fission yeast – a long way from the early days of using a ruler to measure the length of yeast cells on ... could profitably be applied to the growth of Escherichia coli The limitations of synchronous cultures in hiding the fine detail of increases in volume or area could well mean that single cell studies...

Ngày tải lên: 13/08/2014, 22:22

... of the role of PrP in breast cancer metabolism in the study model Picture showing different lactate production in normal and cancer situation Schematic overview of the role of PrP in cancer metabolism ... cancer cell lines (MCF7, SK-BR-3, and MDA-MB-231) Proliferation rate in breast cancer cell lines Up-regulation of p53 in breast cancer cell lines but not MCF7 Low PrP expression in breast cancer cell ... 41 2.6 Cell transfection 42 2.6.1 Dose response curve of MCF7 cells 42 2.6.2 Stable transfection of cell lines using nucleofection 43 2.6.3 Selection of transfected cell clones...

Ngày tải lên: 12/10/2015, 17:36

... adaptations found in cancer cells and offering promising hints for the development of therapies that target metabolic features of cancer cells By making use of the powerful combination of metabolomics ... investigation of the molecular basis of aerobic glycolysis in cancer began, and with it, a new era of research on cancer metabolism The vast majority of metabolic pathways in which cancer cells rely ... cancer cells there is a proportional increase in the amount of both isoforms PKM2 seems to be also predominant in normal adult tissues but the concomitant increase of both isoforms found in cancer...

Ngày tải lên: 22/12/2014, 20:36

... viable cells [19] The percentage of viable cells in the treated samples was estimated, taking, as 100% cellular viability, that of the internal-control nontreated cells Preparation of total nuclear ... [12] Characterization of eEF1A in normal and cancer cells In order to compare the binding properties of the nuclear eEF1A in normal cells compared with those in cancer CCRF-CEM cells, human lymphocytes ... basic nature of the fastermigrating isoform of eEF1A in cancer cells, the estimation of its pI remains merely indicative A magnified image of this analysis, performed with a different nuclear extract,...

Ngày tải lên: 21/02/2014, 00:20

... Intensity of cytoplasm Ratio of Translocation = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software ... Interestingly, the pretreated cells displayed clearly discernible oscillations that closely followed those of the untreated cells in terms of their frequency The effects of exposing the cells to the inhibitor ... assessed in vitro, in cell cultures, and also in silico in this study Previous work [12] demonstrated oscillatory behavior in the levels of nuclear NF-jB in single cell studies In our cell- based experiments,...

Ngày tải lên: 16/03/2014, 11:20