ns3 4 protease and ns5b polymerase

... (–)IRES (–)IRES (–)IRES (–)IRES (–)IRES Kd (nM) 341 DSLA1 D91-97 Dhp2 hp2b LDH2 239 219 1 04 44 64 85 53 97 67 178 344 670 ± ± ± ± ± ± ± ± ± 10 23 11 34 34 47RNA A similar study [20] analyzed the template ... (–)IRES 341 (–)IRES DSL-A1 (–)IRESD91-97 (–)IRESDhp2 (–)IRES hp2b (–)IRES LDH2 (–)IRES 239 (–)IRES 219 (–)IRES 1 04 (–)IRES 100 61 1 64 159 186 131 49 19 24 100 59 ± 1 54 ± 165 ± 173 ± 101 ± 40 ± 13 ... primers used were NS5Bj4s2: 5¢-GATATCATGTCAATGTCCTATACGTGGAC-3¢ and NS5Bj4r 5¢-AAACTCGAGGCGGGGTCGGGCACGAGA CAGG-3¢ The PCR fragment was cleaved with restriction enzymes EcoRV and Xho1 and inserted...

Ngày tải lên: 20/02/2014, 01:20

... et al R16.12 G58 Q41 A59 ● H57 ● S139 T 54 F43 V55 H57 T 54 A/S SCH 44 6211 V55 ligand hydroxyl group S139 L 44 T42 (c) T54A (d) L 44 T54S L 44 S139 F43 F43 S139 ? D81 H57 H57 Q41 Q41 hydrophobic cavity ... entry 2FM2) and the T54A and T54S mutants (all with ligand SCH 44 6211) The wild-type protease structure is shown in green and its ligand in yellow The mutants T54A and T54S are colored red and blue, ... interactions at Q41, F43, H57 and G58 (Figure 4) , but the ligand SCH 44 6211 interacts only with Q41 and H57, but not F43 and G58 No interaction can be observed with the mutational sites V36 and T 54 in the...

Ngày tải lên: 14/08/2014, 08:20

... First she asked me what my name was ,and where I came from Paola : They were easy for you , weren’t they ? Lan: …Then she asked me why I was learning English , and if I spoke any other languages ... 1 Written examination: kì thi viết (an) examiner : Giám khảo (a) candidate : Thí sinh (an) aspect : Lĩnh vực, kĩ ≠ Oral examination : Kì thi nói Was the oral examination ... any other languages Paola : Go on Lan : …Then she asked me how I learned English in my country … And she asked how I would use English in the future Paola : what else did she ask you ? Lan : Oh,...

Ngày tải lên: 11/06/2013, 01:25

... their free time? Thuy Minh and Nam Hung Lan and Dung b) a) d) e) c) f) Unit 12: Lesson 4: B4-5/ p.128- 129 III Dialogue: Listen to the dialogue: Mai Lien Unit 12: Lesson 4: B4-5/ p.128- 129 III Dialogue: ... 12: Lesson 4: B4-5/ p.128- 129 I Vocabulary: - How often: bao lÇn, cã th­êng xuyªn kh«ng - once (adv): lÇn - twice (adv): lÇn - three times (adv): lÇn - a week: tuÇn Unit 12: Lesson 4: B4-5/ p.128- ... Lesson 4: B4-5/ p.128- 129 IV Practice: 1) Substitution drill: one -> once a week two twice a week three three times a week four times a week four five five times a week Unit 12: Lesson 4: B4-5/...

Ngày tải lên: 24/06/2013, 01:26

... asked me what my hoobies were 13- She asked me to look at that picture and describe it 14 She also asked me to read a passage 14- - She asked me what my name was - She asked me where I came from ... asked me what my name was and where I came from She asked me why I was learning English and If I spoke any other languages She asked me how I learned English in my country And she asked me how I ... you are Lan.Complete the dialogue between the examiner and You The examiner You - Goodmorning How are you today? I’m fine, Thanks you, And how are you? - Very well What’s your name? - My name...

Ngày tải lên: 20/09/2013, 09:10

... children b) Don’t be foolish and greedy c) Be happy with what you have d) It’s difficult to find gold HOMEWORK : Do Ex 1, 2, ( page 27–28 workbook ) Prepare : read ( page 41 42 )  ... story and write the letter of the most suitable moral lesson Once a farmer lived a comfortable life with his family His chickens laid many eggs which the farmer used to sell to buy food and clothing ... day, he went to collect the eggs and discovered one of the chickens laid a gold egg He shouted excitedly to his wife, “We’re rich! We’re rich!” His wife ran to him and they both looked at the egg...

Ngày tải lên: 27/09/2013, 22:10

... children b) Don’t be foolish and greedy c) Be happy with what you have d) It’s difficult to find gold HOMEWORK : Do Ex 1, 2, ( page 27–28 workbook ) Prepare : read ( page 41 42 )  ... story and write the letter of the most suitable moral lesson Once a farmer lived a comfortable life with his family His chickens laid many eggs which the farmer used to sell to buy food and clothing ... day, he went to collect the eggs and discovered one of the chickens laid a gold egg He shouted excitedly to his wife, “We’re rich! We’re rich!” His wife ran to him and they both looked at the egg...

Ngày tải lên: 28/09/2013, 19:10

... (+) S + used to + v… Past and present Live in cottages / small houses Live in / big/ modern houses Past and present walk ( in bare feet ) Go / travel by motor Past and present Work on the farm ... to get up late Now, I get up early and morning exercise 1.Make sentences with “ USED TO “ Write sentences of SPEAKING / P 40 on your notebooks Exercise / P 45 Minh Last year, I used to get up ... suitable moral lesson a Don’t kill chickens b Don’t be foolish and greedy c Be happy with what you have d It’s difficult to find gold Past and present Play catch Play video games III Speak Example:...

Ngày tải lên: 28/09/2013, 21:10

... Great- grandma often cooked the meal and wash the clothes T F T There was electricity in Nga’s Greatgrandma’s time T F F F Grandma: Nga : Grandma: Nga : Grandma: Nga : Grandma: Nga : Grandma: Nga ... Nga’s great grandmother What did Nga’s great grandmother and great grand father after dinner? and great grand father after dinner? she lit the lamp and he used to tell she lit the lamp and he used ... had to stay home and help her mom 10 11 12 13 14 15 Thời gian What did Nga’s Great –grandma use to She used to cook the meal , clean the house and wash the clothes 10 11 12 13 14 15 Thời gian What...

Ngày tải lên: 30/09/2013, 04:10

... rich! We’re rich!” His wife ran to him and they both looked at the egg in amazement The wife wanted more, so her husband decided to cut open all the chickens and find more gold eggs Unfortunately, ... children b) Don’t be foolish and greedy c) Be happy with what you have d) It’s difficult to find gold HOMEWORK : Do Ex 1, 2, ( page 27–28 workbook ) Prepare : read ( page 41 42 )  ... the pictures Talk about the way things used to be and the way they are now Model: People used to live in small houses Now they live in big houses and buildings Past People used to work hard all...

Ngày tải lên: 11/10/2013, 06:11

... Property Middle Last Modified Tue, 23 May 2000 15 :47 :43 GMT Mon, 22 May 2000 20 :40 :48 GMT Tue, 23 May 2000 15 :47 :44 GMT Tue, 23 May 2000 15 :47 :45 GMT Tue, 23 May 2000 16:21:55 GMT Tue, 23 May ... installed at your site, create and delete digital dashboards and Web Parts, set properties of digital dashboards and Web Parts, and set permissions on digital dashboards and Web Parts For more information ... use of Internet and intranet content and business data Module 4: Creating and Deploying Digital Dashboards Overview Slide Objective To provide an overview of the module topics and objectives...

Ngày tải lên: 27/10/2013, 07:15

Ngày tải lên: 07/11/2013, 18:11

... or Windows Explorer, and note the presence of AReverser and its properties Module 4: Deployment and Versioning 41 ! Compile, build, and execute the application From a command prompt window, navigate ... publickey and version numbers assembly AReverser assembly AReverser { { publickey = (00 24 82 B1 F2 A0 ) publickey = (00 24 82 B1 F2 A0 ) hash algorithm 0x000080 04 hash algorithm 0x000080 04 ver ... NET Framework SDK documentation  34 Module 4: Deployment and Versioning Packaging and Deployment Tools Topic Objective To provide a brief overview of packaging and deployment tools that are included...

Ngày tải lên: 10/12/2013, 16:15

... Publishing and Uploading a Catalog, 242 0A_04d005.avi To prepare for this module: ! Read all of the materials for this module ! Complete the practices and lab iv Module 4: Designing and Publishing ... (EDI) standards American National Standards Institute (ANSI) X.12 and Electronic Data Interchange for Administration, Commerce and Transport (EDIFACT) Organizations use xCBL to preserve and use ... Finally, you will decide on the structure and format of the catalog and how to deliver the catalog electronically to trading partners  34 Module 4: Designing and Publishing Catalogs Exercise Creating...

Ngày tải lên: 10/12/2013, 16:15

... 10 248 10 248 10 248 10 248 10 249 10 249 10 249 productid 1 1 1 0 0 0 NULL 11 14 42 51 72 NULL 11 42 72 NULL 14 51 (13 row(s) affected) total_quantity 0 0 0 0 76 12 10 40 27 12 10 49 40 22 Module 4: ... productid total_quantity NULL 10 248 10 248 10 248 10 248 10 249 10 249 10 249 NULL NULL 11 42 72 NULL 14 51 76 27 12 10 49 40 (8 row(s) affected) Module 4: Grouping and Summarizing Data 17 Using the ... productid GO Result orderid productid total_quantity 10 248 10 248 10 248 10 249 10 249 11 42 72 14 51 12 10 40 (5 row(s) affected) 16 Module 4: Grouping and Summarizing Data Example This example adds the...

Ngày tải lên: 11/12/2013, 14:15

... 53 25 36 70 17 94 47 82 71 10 E000002.log E000003.log 44 18 13 11 22 12 35 14 40 16 99 19 33 31 20 52 21 E0000 04. log 15 23 42 26 34 46 29 61 48 Circular logging uses transaction log techniques ... understand where files are stored and the various file extensions that ESE uses Make sure students understand how to use log files and circular logging ! Creating and Managing Storage Groups and ... ONLY Module 4: Creating and Managing Storage Groups and Stores 23 # Creating and Managing Storage Groups and Stores Topic Objective To explain how to create a storage group, create and configure...

Ngày tải lên: 21/12/2013, 05:18

... 15 34, Interoperation Between DHCP and BOOTP, and RFC 1 542 , Clarifications and Extensions for the Bootstrap Protocol, under Additional Reading on the Student Materials compact disc Module 4: ... RFC 15 34, Interoperation Between DHCP and BOOTP • RFC 1 542 , Clarifications and Extensions for the Bootstrap Protocol • RFC 2131, Dynamic Host Configuration Protocol • RFC 2132, DHCP Options and ... winipcfg command to release an IP lease 10 Module 4: Implementing and Managing DHCP Requirements for DHCP Servers and Clients Slide Objective To describe the requirements for DHCP servers and clients...

Ngày tải lên: 21/12/2013, 06:15

... "Northwind Traders" OrgName = "Northwind Traders" ProductID = 12 345 - 543 21-12 345 - 543 21-12 345 Next> Cancel ProductID = 12 345 - 543 21-12 345 - 543 21-12 345 You can reduce the amount of configuration information ... Commands, OEM Branding, Additional Files or Folders, OEM Duplicator String, and Answer File Name pages r On the Completing the Setup Manager Wizard page, click Finish  24 Module 4: Creating and ... imaging process to install and configure Windows 2000 Professional Lab Setup To complete this lab, you need two blank, 1 .44 -megabyte (MB) floppy disks 15 16 Module 4: Creating and Deploying an Image...

Ngày tải lên: 17/01/2014, 08:20

... Mod4\Setup.bat, which will create the following virtual directories: a Open Windows Explorer b In Windows Explorer, expand My Computer, expand Local Disk (C:), expand MOC, expand 2295A, expand ... than CGI applications 4 Module 4: Installing and Configuring Web Applications Creating and Removing Web Applications Topic Objective To describe the procedures for creating and removing Web applications ... expand Component Services, expand Computers, expand My Computer, and then double-click COM+ Applications c On the View menu, click Status View d In the IIS snap-in, in the console tree, expand...

Ngày tải lên: 24/01/2014, 10:20

... 45 .95 50–1.50 37 010 6.0 (46 .2) 16.8 (2 .4) 99.7 (99.2) 10–1.50 14. 9 19.7 152 (128.5) 59.51 86.88 45 .44 50–1.85 18 138 9.7 ( 34. 5) 15.8 (5.8) 93.2 (76.6) 10–1.85 19.9 23.6 84 ( 84) 57. 94 85.91 46 .10 ... FEBS Journal 277 (2010) 3699–37 14 ª 2010 The Authors Journal compilation ª 2010 FEBS 3713 HIV protease mutants altering hydrophobic clusters 41 42 43 44 45 46 47 48 49 50 51 52 C-H Shen et al important ... Thr80′ Thr80’ D E I84V Leu23 I54V Val 54 4. 2 3.7 Asp25 3.5 4. 2 Val82 3.6 4. 1 3.7 Ile50 3.7 Val 84 4.0 Ala28 3.5 4. 2 3.3 4. 1 Val32 Thr80′ Fig Interactions of Ile50, Ile 54 and Thr80¢ (A) PRWT–APV compared...

Ngày tải lên: 18/02/2014, 04:20