... typical positive ion spectrum of a single protein consists of a series of peaks, each of which represents a multiply charged ion of the intact Injection of the total mixture of phycobilisomes from Synechocystis ... numbers The left inset of (B) shows the spectra of the four main peaks obtained by the diode array detector The right inset of (B) reports the SDS/PAGE of phycobilisomes recorded by scanning the first ... phycoerythrin in rods of certain phycobilisomes to improve light harvesting [8] Furthermore, modulation of the energy levels of the four chemically different bilins by a variety of influences produces...
Ngày tải lên: 08/03/2014, 16:20
... 9th leaf of different palm trees (last leaf fully opened is numbered ) Light was the only limiting factor Relative error of measurements in low light was too high to allow a comparison of apparent ... Field C & Vasquez-Yanes C (1984) Photosynthetic characteristics of wet tropical forest plants In: Physiological Ecology of Plants of the Wet Tropics (Medina E., et al., eds.), Dr W Junk Pubi., ... Caemmerer S & Farquhar G.D (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves Planta 153, 376-387 von ...
Ngày tải lên: 09/08/2014, 04:20
Báo cáo y học: "Medical resource utilization among patients with ventilator-associated pneumonia: pooled analysis of randomized studies of doripenem versus comparators"
... resource utilization associated with P aeruginosa at baseline reported in other studies [4-6] because of activity against key pathogens Consideration of the influence of appropriateness of empiric ... Discussion The results of this pooled analysis of two phase III studies indicated that initial use of doripenem in patients with VAP was associated with shorter durations of mechanical ventilation ... resource utilization endpoints were analyzed by comparison of KaplanMeier curves with Gehan's generalization of the Wilcoxon test [22], whose weighting scheme reduces the relative contribution of patients...
Ngày tải lên: 25/10/2012, 10:02
Inactivation of microorganisms in untreated water by a continuous flow system with supercritical CO2 bubbling
... the SC -CO2 treatment had disappeared Hong and Pyun (1999, 2001) found that a possible leakage of cytoplasm was caused by the modification of the cell membrane of L plantarum by pressurized CO2 In ... substrate of 0-40 nmoles and are the means with standard errors of the results of triplicate experiments RESULTS AND DISCUSSION Inactivation of microorganisms in untreated water by SC -CO2 treatment ... efficiency of contact between CO2 and the microorganisms (Zhang et al., 2006) Therefore, it was considered that our present result was obtained by the difference of the penetration of CO2 into...
Ngày tải lên: 05/09/2013, 09:38
Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
... (STP) is one of the major sources of EDTA (Kari and Giger, 1996) This sampling site is located at about 150 m downstream from the effluent output of an STP and the contribution of effluent to the ... hand, a standard stock solution of CyDTA was prepared by dissolving 0.01 g of trans-1, 2-cyclohexanediaminetetraacetic in 100 mL of M sodium hydroxide Extraction of EDTA from water samples was ... solutions were of analytical grade Milli-Q water was used in all experiments Gas chromatography/mass spectrometry analysis Gas chromatography was carried out by using HP6890 series gas chromatography...
Ngày tải lên: 05/09/2013, 10:15
Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
... mechanism of the reductive carboxylation of acetyl-CoA (Fig 5) (1) The TPP carbanion is generated by proton extraction from C2 carbon atom of the thiazolium ring by the tautomeric 4¢ imino group of ... activity was defined as the reduction of lmolÆmin)1 of NADH (corresponding to the carboxylation of lmolÆmin)1 of acetyl-CoA) Quantification of CoA The concentration of CoA was quantified using DTNB, ... the addition of CoA solution) The Km value for CoA of the OGOR enzyme is reported to be 80 lm [30] Thus, decarboxylation of 2-oxoglutarate can proceed as a result of CoA contamination of the acetyl-CoA...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... effects of one of the substrates, i.e pantoate [8,9] One of the interesting features of the plant PSs is the presence of a conserved 24 residue insertion in the sequence [9] This sequence of amino ... we report the results of the structural studies of the pantoate-bound form of the N-terminal domain of E coli PS (nPS) The structure and substrate-binding interactions of E coli nPS have been ... the alleviation of this inhibition by another substrate of the enzyme, ATP, are presented [8,9] Results Solution properties of the N-terminal domain of PS, the C-terminal domain of PS, and full-length...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... transition of CRPW85A on binding of cAMP The effect of different concentrations of cAMP on the uorescence intensity of Trp13 is shown in Fig 2B When 200 lM cAMP was added to the solution of CRPW85A, ... the mobility of helix A of the protein As Trp13 is located in the vicinity of the activation region AR2 of the protein [1], which is responsible for the activation of the second class of E coli ... decreases by about A to 18.7 A on binding of cAMP to the anti-cAMP-binding sites of the protein The distance between the sulfur atom of Cys178 and the C9C10 bond of the indole ring of Trp85,...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... [15N]-labelling of selected residues was suppressed by the addition of amino acids at natural isotopic abundance [17] In the case of cell-free protein synthesis, however, the costs of the [15N]labelled ... used undiluted By recording [15N]-HSQC and 2D HNCO spectra of each sample, [15N]-HSQC cross-peaks could be assigned not only by amino acid type, but also by the amino acid type of the residue ... studies of proteins in solution Biochemistry 21, 6273–6279 Le Master DM & Richards FM (1985) 1H-15N heteronuclear NMR studies of Escherichia coli thioredoxin in samples isotopically labeled by...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... matrix of globular protein by diffusion, which is facilitated by small-amplitude uctuations in the protein structure [25,26] The process of quenching the uorescence of Trp residues in protein by ... contributions of the uorescence of the red and blue components from the solute quenching experiments by using the fractional contributions of the short and long lifetimes of the Trp13 residue obtained by ... values of the average Trp13 lifetime instead of the values of lifetimes of the resolved components Binding of cAMP to anticAMP-binding sites leads to signicant changes in the uorescing properties of...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... Intensity of cytoplasm Ratio of Translocation = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software ... with 100 lm of the SC-514 inhibitor We examined the effect of SC-514 treatment on NF-jB activation by stimulating A549 cells with IL-1a for 400 In the presence of SC-514, the kinetics of NF-jB activation ... cell-based measurements of time-varying phenomena, in this case, oscillatory variations of nuclear levels of NF-jB In order to attain this degree of precision, optimization of experimental conditions...
Ngày tải lên: 16/03/2014, 11:20
gas sensing properties of nanocrystalline tungsten oxide synthesized by acid
... ppm NH3 gas Fig 10 The response of DPt 800 (sensor prepared from Pt doped powder D sintered at 800 ◦ C) to 800 ppm of different gases (NH3 , LPG, CNG, CO ethanol and NO2 ) Fig The response of DPt ... measurement of XRD One of the authors (VS) thanks Council of scientific and industrial research for the award of Research Associateship References [1] W Göpel, Ultimate limits in the miniaturization of ... similar to increase in response of tungsten oxide sensor with decrease in grain size as reported by Tamaki et al [13] The sensing properties of WO3 film are also controlled by the surface defects [18]...
Ngày tải lên: 19/03/2014, 16:48
xps studies of active elements surface of gas sensors based on wo3nanoparticles
... measured by means of system, which consists of measuring camera, multimeter Keithley-2010 and PC Results and discussion The XPS-spectra of W4f- and O1s-levels of the samples and the results of their ... spectra of O1s-level were decomposed into separate peaks, FWHM = 1.4 eV The decomposition was carried out by Gauss–Newton method The area of peaks was determined after subtraction of background by ... the results of decomposition of W4f-line in the annealed and non-annealed samples The maxima of peak couples correspond to W4f7/2 - and W4f5/2 -levels of tungsten atoms for W5+ -states of oxide...
Ngày tải lên: 20/03/2014, 13:12
Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc
... number of the diverse superfamily of GPCRs, not least among the prostanoid receptor subfamily of GPCRs [53] The EP3 subtype of the PGE2 receptors is one of the most heavily spliced among all of the ... E1 and E1b RESULTS Analysis of 5¢ UTR of TPa and TPb Identification of the major TP mRNA transcripts and determination of their transcription initiation sites Previous studies have demonstrated ... consistent with splicing of either E1 ()84) or E1b ()84) to E2 ()83) Fig Analysis of differential 5¢ UTR utilization by TP mRNA transcripts (Panel A) Relative positioning of the oligonucleotides...
Ngày tải lên: 31/03/2014, 09:20
Báo cáo toán học: " Structural, optical and magnetic studies of manganese-doped zinc oxide hierarchical microspheres by self-assembly of nanoparticles" docx
... confirmed by SAED studies (inset of Figure 2d) The XPS spectra were used to investigate the composition and chemical bond configuration of the Zn0.93Mn0.07O HMSs The binding energies are calibrated by ... the surface morphology of the products was investigated by scanning/transmission electron microscopy [SEM/TEM] or by high-resolution TEM [HRTEM] The structure was studied by X-ray diffraction [XRD] ... further treatment In a typical process, mmol of mixed reactants of [Zn(CH3COO)2·2H2O] with different amounts of [Mn(CH3COO)2·2H2O] was dissolved in 30 mL of EG The mixture was stirred vigorously...
Ngày tải lên: 20/06/2014, 20:20
báo cáo hóa học:" Structural, optical and magnetic studies of manganese-doped zinc oxide hierarchical microspheres by self-assembly of nanoparticles" docx
... confirmed by SAED studies (inset of Figure 2d) The XPS spectra were used to investigate the composition and chemical bond configuration of the Zn0.93Mn0.07O HMSs The binding energies are calibrated by ... the surface morphology of the products was investigated by scanning/transmission electron microscopy [SEM/TEM] or by high-resolution TEM [HRTEM] The structure was studied by X-ray diffraction [XRD] ... further treatment In a typical process, mmol of mixed reactants of [Zn(CH3COO)2·2H2O] with different amounts of [Mn(CH3COO)2·2H2O] was dissolved in 30 mL of EG The mixture was stirred vigorously...
Ngày tải lên: 21/06/2014, 17:20
Báo cáo hóa học: " Deposition of Size-Selected Cu Nanoparticles by Inert Gas Condensation" pptx
... materials, the source is capable of producing clusters consisting of a few tens of atoms up to particles with diameters of around 20 nm Due to the nature of the gas aggregation technique, narrow ... nucleation of these small cluster ‘seeds’ is followed by the growth of the seeds into larger clusters The growth of clusters is heavily dependent of interatomic collisions; this states the importance of ... probability can be increased by increasing the time spent by a cluster within the growth region So, an increase in the gas flow rate of the carrier gas and, hence, the drift velocity of the material within...
Ngày tải lên: 22/06/2014, 00:20
Báo cáo hóa học: " Optimization, Yield Studies and Morphology of WO3 Nano-Wires Synthesized by Laser Pyrolysis in C2H2 and O2 Ambients— Validation of a New Growth Mechanism" pdf
... schematic of the solid–vapour–solid mechanism of growth of 1D nano-structure from a spherical layer of atoms in a tip growth 123 This corresponds to the number of atoms in the ith segment of the rod of ... CuKa wavelength of 0.154184 nm The experimental procedure showed good reproducibility of results Lengths and corresponding diameters of the nano-wires were measured by means of a software package ... assembled into the rod of length l1, the next layer in the sphere has a radius of R0-X which forms the next segment of the rod of length l2 The subsequent layers have radii of R0 - 2X, R0 - 3X,...
Ngày tải lên: 22/06/2014, 01:20
Báo cáo hóa học: " Effects of Gas Composition on Highly Efficient Surface Modification of Multi-Walled Carbon Nanotubes by Cation Treatment" pptx
... the surface of the tubes The ratio of H2 to O2 is critical to the process because it directly correlates with the composition of the generated cations In this study, the effect of gas composition ... functionalization process is investigated, by providing various H2/O2 gas mixtures to the treatment through the control of gas flow ratio Furthermore, the manner in which the gas conditions correlate with ... output power of approximately 750 W was inserted into the plasma chamber through a quartz window H2 and/or O2 gas mixtures were fed as etching gases with controlled gas flow ratios of 50/0, 40/10,...
Ngày tải lên: 22/06/2014, 01:20
Báo cáo vật lý: "Electrochemical Studies of Mild Steel Corrosion Inhibition in Aqueous Solution by Uncaria gambir Extract" doc
... sample; all of the samples were immersed for 60 over a frequency range of 100 kHz–1 Hz with a signal amplitude perturbation of mV and a scan rate of mVs–1 Next, it was fitted with sets of circuits ... concentration of ethyl acetate extract of U gambir Inhibition efficiency (%) Inhibition efficiency vs pH pH Figure 2: The relation between percentage of inhibition with pH of ethyl acetate extract of U ... presence of various concentration of ethyl acetate extract of U gambir Table 3: Corrosion parameters for mild steel in pH aqueous solution with absence and presence of various concentration of ethyl...
Ngày tải lên: 07/08/2014, 14:20