nodes spleen cells preparation of supernatants and serum collection
Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx
Ngày tải lên :
09/08/2014, 06:23
... in situ effect of licofelone on the gene expression and protein synthesis of the major collagenolytic enzymes (MMP-13 and cathepsin K) and aggrecandegrading proteases (ADAMTS-4 and ADAMTS-5) in ... score of 5.1 (3–11) and a safraninO score of (0–4) Specimens from licofelone-treated groups had a Mankin score of 3.5 (0–10) and a safranin-O score of 0.4 (0–3) with the 2.5 mg dosage and a Mankin ... section The total number of cells and the number of cells that stained positive for the specific antigen were determined The results were expressed as the percentage of cells that stained positive...
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Senescence and Cell Cycle Control
Ngày tải lên :
25/10/2013, 21:20
... different types of cells and in different species Activation of p53 could result in one of the two cell fates, senescence or apoptosis Loss of p53 function could contribute to immortalization and enhanced ... Studies using acute disruption of p21Cip1 by conditional gene targeting will be informative for better understanding of the role of p21Cip1 in senescence of mouse cells Role of the Rb Pathway in Senescence ... complex with cyclins E and A (Sherr and Roberts 1999) In addition to Cdk inhibition, p21Cip1 appears to affect expression and functions of various proteins, which may lead cells to senescence in...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
Ngày tải lên :
19/02/2014, 05:20
... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM ... CD spectrum of C-LytA at 20 °C and pH 7.0, showing two maxima at 265 nm and 290 nm Upon addition of 20 mm choline (a saturating concentration of ligand), two minima at A B 284 nm and 294 nm appear, ... Inhibition of choline-binding proteins Fig Titration of the CD signal of C-LytA at 295 nm with ligands Symbols represent choline (d), atropine (n) and ipratropium (j) (A) Full range of ligand concentration...
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Báo cáo khoa học: The dipeptidyl peptidase IV family in cancer and cell biology pot
Ngày tải lên :
06/03/2014, 09:22
... lymph node, spleen, liver and lung, as well as in pancreatic acinar cells, adrenal gland, spermatogonia and spermatids of testis, and in Purkinje cells and in the granular layer of cerebellum ... kidney; by endothelial capillaries; by acinar cells of mucous and salivary glands and pancreas; by the uterus; and by immune organs such as thymus, spleen and lymph node [22–25] (Fig 2) Our recent ... glutamate-rich region (EE-helix) and stabilized by salt bridges (R-loop) [162,164] Analysis of the crystal structures of DPIV, FAP and DP6, and of the models of DP8 and DP9, indicate that the R-loop...
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Understanding the participatory news consumer - How internet and cell phone users have turned news into a social experience pdf
Ngày tải lên :
06/03/2014, 21:20
... news entirely offline, the same is true of just 38% of non-Hispanic whites and 32% of Hispanics Because they represent such a large segment of internet users, the demographic profile of online news ... online? The act of sharing of news and conversation about news is an integral part of email exchanges and social media activity Of the 71% of the adult population who get news online, 75% of them say ... in terms of their high income and education levels, their young age, their racial/ethnic identity, and their use of broadband and wireless (see table below) Throughout this section and the report,...
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Báo cáo khoa học: Linking pseudouridine synthases to growth, development and cell competition potx
Ngày tải lên :
06/03/2014, 22:21
... by affecting the size and the number of cells To determine whether the reduced wing size of mfl knockdown flies resulted from a decrease in the size and ⁄ or in the number of cells, we performed ... patterning of the wing margin In wild-type discs, signalling between V and D cells resulted in the formation of a band four or five cells wide at the D–V border, which was marked by a central stripe of ... silencing of an independent gene The two lines, named 34597 and 34598, exhibited a silencing A Fig Structure and expression of mfl-silencing constructs (A) Schematic structure of the four mfl mRNA isoforms...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... l-glutamine and 5% fetal bovine serum) and maintained at 37 C and 5% CO2 For translocation experiments, cells were removed with 0.05% trypsin EDTA, and plated with cell solution of ã 106 cells mL)1 ... Intensity of cytoplasm Ratio of Translocation = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software ... Immunocytochemical staining of A549 cells and analysis of the dynamics of NF-jB nuclear translocation (A,B) Doseresponse data from A549 stimulated with IL-1a and xed with formaldehyde (A) and MeOH EtOH ...
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Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot
Ngày tải lên :
16/03/2014, 22:20
... Inhibition of uPAR by DNAzymes all of which contribute to focal proteolysis and regulation of wound healing, angiogenesis, and metastasis [5] Of the components of the uPA system, uPAR is of particular ... After h of serum starvation in the presence of the DNAzyme–Lipofectamine 3580 Total RNA was isolated from Saos-2 cells with TRIzol Reagents using standard protocols of Invitrogen Aliquots of 20 ... untreated and scrambled mutant controls (P < 0.01) (Fig 6) Saos-2 cells treated with the active Dz720 had 42% and 17% fewer cells on the lower side of the filter compared with untreated control cells and...
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Anatomy, Histology, and Cell Biology PreTestTM Self-Assessment and Review pptx
Ngày tải lên :
22/03/2014, 21:20
... proximal and distal tubule cells of the kidney and striated duct cells of the salivary glands Those cells are involved in extensive ion transport CONNECTIVE TISSUE Connective tissue consists of cells ... University of Kansas, School of Medicine Class of 2009 Michael Curley University of Wisconsin School of Medicine and Public Health Class of 2007 Nathan Heckerson University of Kansas, School of Medicine ... phagocytosis), mast cells (the source of heparin and histamine), and a variety of transient blood cells: lymphocytes (B and T), eosinophils, basophils, and neutrophils (PMNs) B cells are involved...
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Targeting New Pathways and Cell Death in Breast Cancer Edited by Rebecca L. Aft docx
Ngày tải lên :
23/03/2014, 17:20
... and the isolation and identification of the ER protein by Toft and Gorski occurred in 1966 (Toft and Gorski 1966) The mediating role of the ER in the estrogen responsiveness of breast Estrogen-Induced ... growth of mammary glands and tumorigenesis, and that castration of immature female mice has delayed the evolution of mammary tumors (Lathrop 1916) However, the chemical control mechanisms of breast ... MCF-7 cells deprived of estrogen for up to 24 months (MCF-7LTED) in vitro expressed high levels of Fas compared to the parental MCF-7 cells, which not express Fas and treatment of the MCF7/LTED cells...
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Báo cáo khoa học: ERK and cell death: Mechanisms of ERK-induced cell death – apoptosis, autophagy and senescence doc
Ngày tải lên :
29/03/2014, 08:20
... pathway-controlled activation of caspases and nucleases leading to the death of the injured cells without affecting neighboring cells The intrinsic pathway of apoptosis regulates the activity of the Bcl-2 family ... immortalized cells, it is also tightly associated with senescence of primary cells In human and rodent primary fibroblastic and melanocytic cells, senescence is triggered by constitutively active forms of ... of autophagy, such as induction of LC3 and conversion of LC3-I to LC3-II [48,49,118], induction of beclin [48], induction of BNIP-3 [66] and activation of G-interacting protein (GAIP) by phosphorylation...
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Báo cáo khoa học: ERK and cell death: ERK1⁄2 in neuronal death Srinivasa Subramaniam1 and Klaus Unsicker2 pptx
Ngày tải lên :
29/03/2014, 08:20
... respective ligand binding In addition, stimulation of endogenous EGF receptors promotes transient ERK1 ⁄ and proliferation, and EGF receptor overexpression induces sustained ERK1 ⁄ and promotion of differentiation ... is often linked to an activation of the ERK1 ⁄ pathway ROS-induced ERK1 ⁄ activation has been demonstrated in a wide variety of cells, including neurons [22] Oxidants induce neuronal death and ... models of neuronal death The first evidence for a role of ERK1 ⁄ in cell death was demonstrated in the oligodendroglial cell line, CG4 The addition of H2O2 to CG4 cells resulted in activation of all...
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Báo cáo khoa học: ERK and cell death: ERK location and T cell selection pdf
Ngày tải lên :
29/03/2014, 08:20
... of its regulation is still unclear Negative selecting ligands induce a rapid and robust induction of phopho-ERK1 ⁄ 2, whereas positive selecting ligands induce slow and sustained activation of ... activity and subcellular location are the same regardless of the ligand strength, ERK location and T cell selection whereas ERK activity and location change depending of the nature of the selecting ... Phosphorylation of these tyrosines facilitates the recruitment of phospholipase C (PLC)c1, Gads, SLP-76 and Grb-2 PLCc1 is essential for the mobilization of calcium The role of calcium, upstream of calcineurin,...
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Báo cáo khoa học: ERK and cell death: cadmium toxicity, sustained ERK activation and cell death pot
Ngày tải lên :
29/03/2014, 08:20
... macrophages, primary calvaria cells, HeLa cells, HEK293 cells, C2C12 cells) , a strong ERK activation was observed 16–24 h after the addition of micromolar concentrations of cadmium This activation ... Alternatively, cells were cotreated with 2.5 lM of the PKC inhibitor GF 109203X and 100 ngÆmL–1 PMA and cadmium (GFX ⁄ PMA ⁄ Cd 24h) or pretreated with GF 109203X and PMA for h and then treated ... sustained activation of ERK could be a response of the cells to survive following cadmium poisoning However, treatment of intoxicated cells with U0126, a potent and specific mitogenactivated protein kinase...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
Ngày tải lên :
30/03/2014, 13:20
... phosphorylation of p130 Cas and Fak and increases attachment and spreading of preosteoblastic MC3T3–E1 cells Bone 30, 99–108 37 Imamura, F., Mukai, M., Ayaki, M & Akedo, H (2000) Y-27632, an inhibitor of ... [30] In order to test the effects of LPA on PAK1 activity in A2058 human melanoma cells, serum starved cells were stimulated by lM LPA for various periods of time, and PAK1 activity was accessed ... activation in B103 neuroblastoma cells [34] Therefore, the possible involvement of Fig LPA stimulates PAK1 in A2058 cells The cells were serumstarved for 12 h and then stimulated with lM LPA for...
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Báo cáo khoa học: PKA independent and cell type specific activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx
Ngày tải lên :
30/03/2014, 16:20
... activities in GLUTag and InR1G9 cells, and PKAc transfected InR1-G9 cells were assayed Approximately 3.0 mg total cell lysates were utilized for immunoprecipitation, and one third of the precipitate ... endocrine cells FEBS Journal 272 (2005) 2746–2759 ª 2005 FEBS L Chen et al Activation of Cdx-2 expression by cAMP Fig Comparison of the effect of H-89 on expression of the proglucagon and Cdx-2 ... The effect of forskolin ⁄ IBMX and Epac pathway specific cAMP analogue on the phosphorylation status of ERK1 ⁄ and Cdx2 protein expression A InR1-G9 cells were grown in the presence of ethanol...
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schaum's easy outline molecular and cell biology - william stansfield, raul j cano, jaime s. colome
Ngày tải lên :
08/04/2014, 12:52
... adhesiveness of bacteria and the facilitation of conjugation (see Chapter 7) One of the distinguishing features between plants and animals is that plants and fungi have cell walls made of cellulose and ... microtubules of the spindle bundle and function to distribute chromosomes as cells proliferate Propagation and maintenance of any piece of DNA requires the presence of one or more origin of replication ... of a substrate, two major electron carriers, NAD+ and FAD, become reduced to NADH and FADH2 One complete turn of the TCA produces three molecules of NADH, two molecules of CO2, one molecule of...
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