nmr characterization of the macromolecular constituents of cereals
Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
Ngày tải lên :
23/03/2014, 13:20
... with the calculated monoisotopic [M-2CO2-H]– mass of 1840.42 Da) The mass shift of Da (MR ) M) confirms the formation of the two disulfide bridges However, comparison of the retention times of the ... that even in the synthesis of nondisulfide bridge-containing glycopeptides the yield is dramatically affected by the scale of the reaction, the excess of amino acids used, and the level of purity ... did not further determine the configuration of this carbohydrate However, the different elution profiles of the native tx5a and the synthetic peptides constructed with the carbohydrate in the T-antigen...
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Báo cáo khoa học: Quenched hydrogen ⁄deuterium exchange NMR characterization of amyloid-b peptide aggregates formed in the presence of Cu2+ or Zn2+ ppt
Ngày tải lên :
30/03/2014, 01:20
... between the fibrillar and the metal-bound aggregate forms is therefore of interest with respect to understanding the mode of assembly and pinpointing the mechanism by which metals guide the path of ... formed in the presence of a molar excess of divalent metal ions failed to attain the classic morphology of amyloid fibrils [37,46] The aggregates of Ab(1–40) and Ab(1–42) formed in the presence of a ... monomers through the addition of a chelating agent, suggesting that their mode of assembly is different from that of the fibrillar forms Quenched H ⁄ D exchange NMR on Ab assemblies The use of quenched...
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Báo cáo khoa học " NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters " pdf
Ngày tải lên :
28/06/2014, 11:20
... branch points on the b-(1 fi 6)-D -glucopyranosyl residues (Scheme 1) The integral of the anomeric H resonances of the a and a0 residues of the backbone compared with the integral of the anomeric ... and NMR spectroscopy, and the results were the same; therefore, only the analysis of the fraction extracted from the mycelium grown on OMWW is reported here The GC analysis, performed on the ... experiment The H and 13 C chemical shift values of these three spin systems suggested the presence of glucose residues (Fig 3) The H and 13 C assignments of these residues are reported in Table The...
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Characterization of Cereals and Flours doc
Ngày tải lên :
11/08/2014, 19:20
... to be detected the experimental time scale should match the time scale of the relaxations in the molecular structure Magnitude of the observable: The magnitude of the change in the physical observable ... (2) The free energy change is obtained from the relation ∆G ϭ ∆H Ϫ T ∆S (3) Taken together these data provide a complete thermodynamic characterization of the material The advantages of the calorimetric ... moisture The two endotherms coalesce above 67% moisture, and the transition temperature of the resultant endotherm is independent of further increase in moisture content The presence of two endotherms...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
Ngày tải lên :
18/02/2014, 06:20
... implications of the observed VpUcyt structure One of the most studied activities of VpU is the induction of CD4 degradation in the endoplasmatic reticulum of infected CD4+ cells Only the cytoplasmic ... regions of the Ramachandran plot An additional 6% of residues were found in the generously allowed region None of the residues of helices and was found in the disallowed regions Instead, Procheck -NMR ... respectively retained in the centre of the interhelical linker and at the C-terminus of micelle-associated VpUcyt Position of VpUcyt relative to the micelle NMR signal intensities of individual VpUcyt...
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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc
Ngày tải lên :
16/03/2014, 14:20
... sources, from tissues of differing ages and also within a single chain Enhanced availability of data to facilitate the characterization of these structures is therefore of value GAGs are not ... p.p.m.), but other signal positions are strongly perturbed compared with their locations in the related tetrasaccharides There is one further connected set of protons present for both of these species ... is indeed found to be the case; only for H1 of ring E in CS060606 is there a relatively strong change, of )0.07 p.p.m., relative to H1 of ring C in CST0606 For all of the other ring E signals in...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
Ngày tải lên :
02/11/2012, 11:08
... interspersed by a stimulus which deviated from the standard in the intensity of the first component and the glide direction of the second constituent The number of distinct MMN components elicited by ... 56]; these findings further support the concept of the P3 as a proxy for some element of stimulus evaluation time Evidence has accumulated describing the P300 as a cognitive routine supporting the ... and the N2b Data indicate that the P300 is involved not only in processes of working memory, but may interact with the N2b in the control of motor response to external cues Through the use of...
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Characterization of the microbial community in the anaerobic/oxic/anoxic process combine
Ngày tải lên :
05/09/2013, 09:38
... from the anoxic tank, and the amount of sludge was adjusted to 9.4 % of total MLSS per day Ozonation was performed twice a week, and then the supernatant of the ozonated sludge was flowed into the ... the sewer line is lower than those in the UCT processes In sequencing batch reactors (SBRs), the PURs are higher than the others (Table 1) These high PURs were due not to the differences in the ... mg/L of SS, 55–80 mg/L of TOC, 45–55 mg/L of T-N, and 4.0–5.5 mg/L of T-P Other details are shown in the related study (Kondo et al., 2009) Sampling was performed once efficient - 156 - Journal of...
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Characterization of the Carbon Stable Isotope Ratio and Fatty Acid Structure of Zostera japonica in Coastal Areas
Ngày tải lên :
05/09/2013, 10:17
... the deciduous effect of the respective parts of Z japonica The δ13C in the roots and rhizomes seems to be larger than the δ13C in the leaves and stems For the same species of seagrass, when the ... (LCFAs) These standards were used to obtain 56 kinds of fatty acids Therefore, the contents of each fatty acid were calculated based on the quantity of lipid, the content ratios of all the fatty ... we think that the characteristics of the fatty acid structure in the leaves and stems differ from those of the roots and rhizomes because the content of the fatty acid 18:3n3 in the leaves and...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
Ngày tải lên :
05/09/2013, 16:11
... the inner part of the membranes As shown in Figure 4e, many big aggregates appear in the middle part of the PVDF-PS membrane The hydrophobic property of PS polymer promoted the formation of these ... Thermal analysis of the PVDF-SPS composite membrane The TG analysis curve is presented in Figure for the investigation of the thermal stability of the PVDF membrane, PVDF-PS membrane, and the PVDF-SPS ... decomposes The swelling is due to the degradation of the cross-linking structure of the membrane in the H2O2 solution [33] The decomposition rate is larger than the swelling effect after 60 h The result...
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Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx
Ngày tải lên :
14/02/2014, 03:20
... wavelength of 1.540600 Å The X-ray data was collected using an MBraun curved PSD with a chamber depth of cm The focal radius of the system was 57 mm, and the cm length of the PSD subtended ∼25° 2θ of the ... however, the assignment of component polymorphs to sample mixtures depended on the XRPD results Conclusions Interconversions of the Polymorphs of Disodium Salt The interconversions of polymorphs of the ... anhydrous thermal event Form I to Form II melt of Form II Form I to Form II melt of Form II loss of water melt of Form I Form I to Form II melt of Form II loss of water melt of Form III melt of Form...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... unclear whether these cytokines are produced by CD4+CD25+Foxp3+Treg or whether they induce the production of these cytokines from another population of cells [20] To date, our knowledge about the different ... important in the generation of the correct class of antibodies by B-cells, and for the elimination of extracellular pathogens, such as helminths and other extracellular parasites [2] Th17 is the most ... providing further evidence of their identity Multiple alignments of the zebrafish t-bet, stat6 and foxp3 amino acids with their vertebrate homologues revealed regions of high conservation These regions...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
Ngày tải lên :
16/02/2014, 09:20
... to study the binding of ATP alone to the protein were unsuccessful, as the protein precipitated upon addition of ATP in the absence of pantoate An important feature of the NMR study is the fact ... regions of the Ramachandran map [32,33] Comparison of the monomer of the substrate-free form of E coli PS [13] with the monomers of nPS in the pantoate-bound form at full occupancy indicates that the ... back to the position of the Fig Superimposition of the A-chain of nPS (green), the B-chain of nPS (cyan), and the substrate-free protein (1IHO.pdb; magenta) The carbon and oxygen atoms of pantoate...
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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc
Ngày tải lên :
18/02/2014, 13:20
... Fig Example of the deconvolution pattern of the methylene region in the 1D 1H NMR spectra of MCF-7 cells (A); integration regions for selected cross peaks in the 2D 1H NMR spectra of MCF-7 cells ... and Fig 3A) to the spectral tting of samples without ML signals The presence of these peaks was paralleled by the existence of lipid cross peaks in the 2D spectra (Table and Fig 3B) The cross peak ... 1H NMR spectra of cells has been studied subsequent to the rst observations being made in cancer cells, lymphocytes and developing cells On the other hand, a correlation of the intensity of these...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
Ngày tải lên :
18/02/2014, 18:20
... For the fraction of substrates that undergo the reaction, the resultant products dissociate from the ribozyme relatively slowly on the time scale of the reaction Furthermore, dissociation of the ... to (if not the same as) the role of the xG in the second step of self-splicing [4856] This suggests that the guanosine to be excised is likely binding to the GBS of the ribozyme for the exon-ligation ... appears that there is room for improvement in terms of the rate of reaction This would be benecial not so much in terms of the rate of the overall reaction, as the cleavage reaction is not the limiting...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
Ngày tải lên :
18/02/2014, 18:20
... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III proteins The ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the ... [14] The side-chains of the first pair of residues, i.e K135 and R148, matched almost exactly; those of the second pair were in close proximity The R148 residue of the Pfu-primase is part of a...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
Ngày tải lên :
19/02/2014, 00:20
... and the remodelling of larval tissues to produce adult tissues (i.e the development of the gills and the production of an adult shell), which is accompanied by significant growth of the soft body ... based on the conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 147 bp sequence Cloning and sequencing of this fragment ... Multiple sequence alignment of Cg-Clp2 with members of the GH18 family (A) The predicted amino acid sequence of Cg-Clp2 is aligned with the amino acid sequence of three CLPs from the oyster C gigas (Cg-Clp1),...
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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
Ngày tải lên :
19/02/2014, 05:20
... constants of the 15N nucleus of the distal NO and of the 14N nucleus of the proximal His of the GmHO-1 complex are closer to those of the rHO-1 complex than to those of the SynHO-1 complex Thus, ... of the heme pocket of GmHO-1 might be associated with the smaller values of Kazide for GmHO-1 and SynHO-1, and with the ferric character of the heme The Kimidazole values of the three kinds of ... against the concentration of azide of each solution The intersection point corresponding to the mole fraction of 0.5 of the respective forms gives the amount of azide necessary to attain the equilibrium,...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
Ngày tải lên :
19/02/2014, 06:20
... fragments F and I The number at the right of each RT-PCR product represents the length of the product The scheme also shows the location of the reverse primer DN+89 used in another starting exon-specific ... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbers in parentheses below the diagram show the position of the corresponding ... is done from +1 of exon )11871 above the sequence indicates the 5¢-end of exon 1a The numbers in parentheses demonstrate the position of the corresponding nucleotides relative to the transcription...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
Ngày tải lên :
19/02/2014, 06:20
... peptides covered 39.1% of the sequence The molecular masses of the tryptic peptides, either from the mass fingerprint or purified peptides, covered 60.3% of the sequence, and the masses of cyanogen bromide ... which these states are alternated, has been proposed [16] The met state is the resting state of the enzyme, and in the absence of substrate about 85–90% of the enzyme is in this state [16] Both the ... changes the redox potential of the phenolic substrates, making them more easily oxidized Therefore, the pH profile reflects not only the optimal behavior of the enzyme, but also changes in the substrate...
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