new technology in muscle gene therapy

... shown in Fig The packaging system contains the self-inactivating transducing constructs (sin) obtained Fig Diagram of lentiviral vector carrying the human IDUA cDNA Gene transfer vector includes ... subcloned into plasmid pRRLsinPPT.CMV.WPRE after double digestion of this vector with MscI and MluI to obtain the self inactivating (sin) gene transfer construct PRRLsinPPT.CMV IDUA.WPRE The packaging ... co-incubated, untransduced cells Lentiviral vectors have been shown to effectively transduce genes into a range of both dividing and nondividing cell types, including neurons, retinal cells, muscle...

Ngày tải lên: 24/03/2014, 04:21

... With this in mind, we compiled this collection of muscle gene therapy methods and protocols with the intention of bridging the translational gap in muscle gene therapy The book is divided into three ... enhancing activity of the cassette and staying within the vector packaging size constraints (e.g., introducing a large insertion into a cassette may not be justified by only a slight increase in ... wasting X-linked genetic disease caused by dystrophin gene mutations Gene replacement therapy aims to transfer a functional full-length dystrophin cDNA or a quasi micro/mini -gene into the muscle...

Ngày tải lên: 29/06/2014, 09:20

... Mata M, Fink DJ In vivo gene therapy for pyridoxine-induced neuropathy by herpes simplex virus-mediated Presumed dog β-NGF gene therapy in vitro and in vivo 373 gene transfer of neurotrophin-3 Ann ... determine the effect of the cytomegalovirus (CMV) vector-mediated gene transfer of the pdβ-NGF in vitro and gene therapy using recombinant pdβ-NGF plasmid in the dog model, having pyridoxine-induced ... of pyridoxine intoxication in dogs In high doses, pyridoxine causes a selective degeneration of large and small myelinated sensory axons in the central and peripheral nerves, resulting in numbness...

Ngày tải lên: 07/08/2014, 23:22

... opportunistic infections There is a great deal of progress in the area of stem cell gene therapy for AIDS [12] A primary goal of many ongoing studies is to introduce an effective anti-HIV gene into hematopoietic ... Decoys in Modeling Stem Cell-based Gene Therapy for HIV/AIDS Anticancer Res 2003, 23:1997-2005 Michienzi A, Castanotto D, Lee N, Li S, Zaia JA, Rossi JJ: RNA-mediated inhibition of HIV in a gene therapy ... hematopoietic cells HIV continues to be a major global public health problem with infections increasing at an alarming rate [10,11] Given the present lack of effective vaccines and the ineffectiveness of...

Ngày tải lên: 13/08/2014, 09:21

... integrating into host cell DNA or remaining episomal inside the cell Wild-type adeno-associated virus has been shown to integrate into chromosome 19 in the host cell by non-homologous recombination ... this method of in utero gene transfer with rAAV can be useful in treating metabolic diseases by providing long-term gene expression A number of experiments involving in utero gene transfer with ... utero rAAVcntf-gfp gene therapy Expression of GFP in lungs at one year of age following in Expression of GFP in lungs at one year of age following in utero rAAVcntf-gfp gene therapy Rats were...

Ngày tải lên: 14/08/2014, 19:22

... promising new cancer therapy approach, gene therapy is a technique which involves introduction or removal of genes within cells to treat diseases Since the first clinical trial involving human gene ... Gene/ Prodrug System Used in Gene Therapy Gene therapy is one of the most promising new frontiers in medical therapeutic intervention, especially in tumor therapy Currently used applications in ... HSVtk/GCV system, thus being more effective in eliminating glioma cells Intratumoral injection of NSCs expressing the CD gene into BALB/c nude mice harboring U87 glioma xenografts induced significant...

Ngày tải lên: 12/10/2015, 17:34

... avoid epigenetic problems Incrementa l improvements in efficiency Ideal improvements Gene targeting via recombinase use Gene targeting by engineerin g RVV genome Gene targeting by engineerin g RVV ... [12, 13], then in of the resulting individuals would contain no transgene sequences, another in would contain transgene sequences in 100% of their cells, and the remaining from individuals would ... germline gene therapy A major problem is the relatively low rate of transgene integration: in mice, the overall efficiency of transgenesis (taking into account embryo loss in vitro and in vivo)...

Ngày tải lên: 03/11/2012, 10:01

... participation in making stories He foresees a new digitized world of playing in which we can be active agents in producing the stories that make the world go round, thus generating new narrative ... as checking e-mail, uploading a movie to YouTube, online role-playing, listening to mp3 music, or using an e-learning environment When it comes to digital material, the lines separating objects, ... soldier Americas Armys main design principle is to create a virtual replica of certain aspects of life in the US Army, mainly those involving combat As an important institution in the American society,...

Ngày tải lên: 16/02/2014, 23:20

Ngày tải lên: 17/03/2014, 17:20

... analogue scale INTRODUCTION The population in Finland and in the developed countries in general is aging rapidly Compared to the rest of Europe, the aging of the population is fastest in Finland (Statistics ... McGill Pain Questionnaire PAINReportIT Impak Health Journal for Pain PIPER DiaryPRO Pain None specified Pain Pain Pain Pain Mood None specified None specified Chronic pain patients in home-care ... the new technology Designing and implementing new technology to make patient monitoring easier involves several challenges Perhaps the most important challenges are the system interface, user interface,...

Ngày tải lên: 28/03/2014, 17:20

... identified in food engineering This book presents topical research in the study of food engineering, including: ozone technology in the food industry; current trends in drying and dehydration of ... CONGRESS CATALOGING -IN- PUBLICATION DATA New topics in food engineering / editor: Mariann A Comeau p cm (Food science and technology) Includes index ISBN 978-1-61209-896-8 (eBook) Food industry and ... controlling moisture migration is to add an edible barrier between components In this study, in order to try to reduce the water activity of raisins, while maintaining their softness, raisins were infused...

Ngày tải lên: 02/04/2014, 15:05

... distinctions in mind we can raise some additional ethics issues regarding gene therapy from a social point of view Is there anything intrinsically morally objectionable about gene therapy in any ... would support a presumption in favor of continuing the development of germline genetic engineering That is, we have argued that germline genetic engineering is not intrinsically morally objectionable ... engaged in germline genetic engineering Failing that, however, we would still have the alternative of creating multiple eight-cell embryos in vitro, genetically analyzing them, then implanting only...

Ngày tải lên: 10/04/2014, 22:10

... transfer genetic information into nondividing cells in vivo In addition, expression has been maintain for up to one year Further experiments to determine if AAV vectors integrate or are maintained in ... Replication-incompetent retroviral vectors can be easily generated by deleting retroviral genes and adding gene( s) of interest Vectors can be produced in packaging cell lines that express packaging genes ... replication of an EBV origin of replication Plasmids containing these replication origins and relevent appropriate proteins activating origins are maintained longer in cells in vitro and in vivo than plasmids...

Ngày tải lên: 10/04/2014, 22:11

... generating increased collateral flow But documented clinical success in reducing angina scores and improving myocardial perfusion in otherwise untreatable patients has been observed In a porcine ... cardiovascular gene therapy Hum Gene Therapy 10:3031–3044, 1999 Mann MJ, Gibbons GH, Tsao PS Cell cycle inhibition preserves endothelial function in genetically engineered rabbit vein grafts J Clin Invest ... 200 GENE THERAPY IN CARDIOVASCULAR DISEASE Mann MJ, Whittemore AD, Donaldson MC Preliminary clinical experience with genetic engineering of human vein grafts: Evidence for target gene inhibition...

Ngày tải lên: 10/04/2014, 22:11

... molecules in the interleukin family, which have similar effects and have also been studied, including interleukin (IL-4), interleukin (IL-7), and interleukin 12 (IL-12) IL-12 is a heterodimer consisting ... translational initiation site or a splicing site on the gene (Fig 10.4) This binding represents an antigene approach that would inhibit genetic information flow (DNA–RNA–protein) The antigene approach ... Lipofection 250 GENE THERAPY IN THE TREATMENT OF CANCER now in development Such inhibition results not only in growth inhibition in vitro but also results in growth inhibition of tumors in animal models...

Ngày tải lên: 10/04/2014, 22:11

... of expressing foreign genes in mammalian cells [8,9], in the early 1980s, AAV2 vectors have rapidly gained popularity in gene therapy applications, due to some of their distinguishing biological ... and overcome, rAAV-based gene transfer vectors still represent one of the most promising gene therapy systems and gain increasing popularity In a search for "AAV gene therapy" , 1,016 PubMed references ... most promising and distinguishing features of AAV vectors However, there are also a few drawbacks in using AAV vectors for gene therapy applications (1) Their size limits the insertion of gene expression...

Ngày tải lên: 18/06/2014, 18:20

... target suicide gene therapy mediated by hTERT promoter in combination with immuno -gene therapy in a murine model of LLC both in vitro and in vivo Xu et al Journal of Translational Medicine 2011, ... (Shanghai, China) and maintained in 5% CO2 at 37°C in Dulbecco’s minimum essential medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin All culture reagents ... and initiating Th1-type immune responses by activation of CD4+ and CD8+ cells [16] In the present study, we investigated a combined target suicide gene therapy and immunomodulating gene therapy...

Ngày tải lên: 18/06/2014, 19:20

... 2011 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org New Perspectives in Biosensors Technology ... Detection Technology Based on MEMS The Sionex Company was founded in the USA in 2000, and is mainly engaged in the research and development of mico-FAIMS detection parts, and it is leading in the ... A, B and C is different in the high electric field The mobility of ion A will increase with an increase in the electric field intensity; when the electric field intensity increases, the mobility...

Ngày tải lên: 19/06/2014, 21:20

... engineering, industrial micro-nanobiotechnology, biomedical technology, bioinformatics, bioengineering, information and telecommunication technologies and etc The sensing element in the form of inorganiс ... (Si, GaAs and InSb) are of a particular interest in terms of developing SAW-based integrated devices with built -in information processing including sensing, executive and processor units Piezoelectronic ... neural, intelligent and sensory networkers In addition, creative persons interested in understanding, modeling, designing of intelligent sensory systems and in development of smart devices, machines...

Ngày tải lên: 19/06/2014, 21:20

... vapor in any spaces is developed which enables to define instantly drinking using not remote labs, but a distributed intellect in multidimensional space of 58 New Perspectives in Biosensors Technology ... during the injection interval This subsequently drops to the level of the baseline when injection is finished The second component, a binding response, corresponds to an increase in mass resulting ... (4,5 g/l), containing 10% FBS supplemented with HAT (100 μM hypoxanthine, 0.4 μM aminopterin and 16 μM thymidine) and antibiotics, at 37 °C in a humidified atmosphere containing 5% CO2 The cells...

Ngày tải lên: 19/06/2014, 21:20