neurochemical and molecular basis of peripheral sensitization

... Bio-2050, Fall 2012 Molecular and Cell Basis of Inheritance NQH, DDL, HTMN @HUS Announcements Recitation takes place sometime (for ca hour) this week, Pls ask Dr Huy where and when exactly? Exam ... 2nd theme Cell Cycle, Mitosis and Checkpoints A wrap-up of chromatin Experiments that established the idea of the cell cycle Mitosis and chromosome movement Control of the cell cycle 3rd theme ... @HUS Overview of today’s class: Reading: Chapter 4, Chapter 11, 14.1, 14.2, 14.3, 18.2 1st theme Nucleic Acids and DNA Packaging DNA as hereditary material Structure and chemistry of nucleic acids

Ngày tải lên: 20/06/2017, 08:26

... Semester Molecular and Cell Basis of Inheritance NQH, DDL, HTMN @HUS Announcements The 2nd practical section on genetics starts this week, in R 235, T1 (Dept Genetics) NQH, DDL, HTMN @HUS Overview of ... 4th theme DNA replication and repair The end replication problem and telomerase How cells repair replication mistakes Other types of DNA repair 5th theme Applications of DNA synthesis in Mol Bio ... 6th theme Meiosis and Genetic Variability Vocabulary of meiosis The process of meiosis Nondisjunction – when good spindles go bad NQH, DDL, HTMN @HUS 4th theme DNA replication and repair NQH, DDL,

Ngày tải lên: 20/06/2017, 08:27

... some of the key findings that have led to our current under- standing of the molecular mode of action of glypho- sate and the molecular basis for glyphosate resistance. Structure and function of ... (Arg21, Arg111, and His138) and with the carboxylate end (Arg21 and Arg73) of GriP. Of partic- ular note is a short, 2.46-A ˚ hydrogen bond between N- e of His138 and a phosphate oxygen of GriP. Alanine ... deamination of primary and secondary amines (sarcosine, N-ethylgly- cine, and glycine) and d-amino acids (d-alanine and d- proline), yielding the corresponding a-keto acid, ammonia or primary amine and

Ngày tải lên: 14/02/2014, 14:20

... tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors Kenji Takeuchi and Fumiaki Ito Department of Biochemistry, Faculty of Pharmaceutical ... causing activation of Bax and Bak [23] Regulation of Bcl-2 family members can occur by a number of mechanisms, including up-regulation of synthesis, enhancement of degradation and phosphorylation ... level of Bcl-2 family genes and the phosphorylation state of their proteins, thereby changing the amount and localization of Bcl-2 family members However, each type of cancer has its own way of

Ngày tải lên: 16/02/2014, 09:20

... mutational rates of the exon of the 3FTx genes are much faster than those of the PLA genes, leading to high variation of KBf-3FTx. Cloning and cDNA sequencing Venom glands of only one of the KBf specimens ... pre- sent in B. candidus venom, while 3FTx-RK is 84% identical to bucain [25] of B. candidus. Calcium binding and kinetic parameter of the P31-PLAs Four PLAs (Va, Vb-2, VI and II) of KBf and Bf con- tain ... P. australis: PA11 and PA13, P04056 and P04057; P. porphy- riacus: pseudexin A and B, P20258 and P20259; and O. hannah: acidic I and II, P80966 and Q9DF33. Venom proteins of Bungarus fasciatus

Ngày tải lên: 16/03/2014, 12:20

... dialyzed solution of AS60 (300 mL) obtained from 100 g of defatted ink powder contained 2.9 g of protein, 0.29 g of hexose, 0.25 g of uronic acid, 0.12 g of methylpentose and 5400 units of tyrosinase ... The molecular masses of the subunits of ST94 and ST94t were similar to those of the proenzymes ( 70–80 kDa) and the activated enzymes by proteolysis ( 60–70 kDa) of a bivalve tyrosinase [34] and ... bond These estimates of the molecular mass of ST94 were supported by the result of gel permeation HPLC (Fig 3) There was a minor band between 140 and 232 kDa in the native-PAGE of Fig 2A (lane 1),

Ngày tải lên: 17/03/2014, 10:20

... of convergent toxin evolution. In this review, we focus on PMT and our current understand- ing of the structure–function, mechanism of action and cellular consequences of this newest member of ... structural and biochemical basis of the effects of these toxins on vari- ous host signaling pathways have provided interesting and sometimes surprising insights into the molecular mechanisms of the ... ARTICLE Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida Brenda A. Wilson and Mengfei Ho Department of Microbiology and Host–Microbe Systems Theme of the

Ngày tải lên: 22/03/2014, 15:21

... Higgins2 and Maria G Tuohy1 Molecular Glycobiotechnology Group, Department of Biochemistry, 2Department of Chemistry, and 3Department of Biochemistry, National University of Ireland, Galway, Ireland; ... was 1.8°, the standard deviation of the hydrogen bond energies was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant ... in position With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops extending from the

Ngày tải lên: 23/03/2014, 13:20

... non- glycosylated monomeric protein with a molecular mass of 14 kDa and a specific activity of 400 UÆmg )1 in the presence of 1 mm sodium taur- odeoxycholate and 4 mm CaCl 2 with phosphatidylcholine ... rate of 1 mLÆmin )1 . Molecular mass markers were used to esti- mate the molecular masses of eluted pro- teins. Right panel: SDS ⁄ PAGE (15%). Lane 1: molecular mass markers. Lane 2: 10 lg of fractions ... investigate the effect of bile salts on ChPLA 2 activity, the rate of hydrolysis of PC by ChPLA 2 in the presence of vari- ous concentrations of bile salts, at pH 9.5 and at 37 °C, was studied. As

Ngày tải lên: 30/03/2014, 01:20

... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viral- derived ... detection of TYLCV and make discoveries to improve recovery of geminiviruses from FTA cards... cDNA to amplify CP and MP sequences for TMV and the conserved region of CP and NiB ... sampling of the viruses infecting plants is required to facilitate their molecular study and is essential for the development of crop protection and improvement programs. Retaining integrity of viral

Ngày tải lên: 19/06/2014, 08:20

... about the storage and realization of genetic information As a sounding illustration of this topic, one of the Brandenburg Concertos was played and in parallel the single pages of the partitur, ... complex Molecular basis of cytokine signalling between the aspartic acid ⁄ lysine mutant of BMP-2 and ActR-IIB The structure does not indicate any new bonds in trans between the ligand and the ... receptor of the ligand, whereas the main core structure binds into the epitope for the type II receptor of the BMP ligand Therefore, the binding of the receptors of both subtypes is blocked and BMP...

Ngày tải lên: 15/03/2014, 09:20

... progressive accumulation of mutations, loss of apoptotic control and regulation of cell proliferation, and the appearance of aneusomy are associated with worsening dysplasia phenotypes and may reflect ... subclasses of adenocarcinomas Selected genes allow the discrimination between primary lung cancer and metastasis of extrapulmonary sites [90] Studies of expression profiles of adenocarcinomas of the ... Tissue microarrays (TMAs) of lung cancer Tissue microarrays (TMAs) of lung cancer TMAs are comprised of core biopsies of 0.6 mm in diameter of different tumors and of uninvolved lung from the...

Ngày tải lên: 12/08/2014, 18:21

... analysis and ligand docking CH performed rotamer analysis and ligand docking GM and AS performed analysis of residue interaction networks CS conceived experiments MA, SZ and TL conceived and designed ... protein-ligand docking Structural changes in the binding pocket and the catalytic triad of the protease were characterized by molecular dynamics simulations of T54A/S mutants and rotamer analysis of ... mutated positions V36 and T54 are buried in the protease domain of NS3-4A in the two β-strands β1 and β3 of an antiparallel β-sheet (Figure 2) T54 is at the very end of the strand β3, next to a loop...

Ngày tải lên: 14/08/2014, 08:20

... Molecular Basis of Siah2 Substrate Specificity Given the different roles of Siah1 and Siah2 in cancer and their different cellular functions, it is important to understand the structural basis ... PHD3 Homology modeling and docking of Siah2 SBD and PHD3 Interaction of chimeric forms of Siah1 and Siah2 SBD with PHD3 The three dimensional (3D) models of Siah2 SBD and PHD3 were prepared by ... Interaction of wild type (WT) and chimeric Siah proteins with PHD3 (a) Diagrammatic representation of the WT Siah1 and Siah2 SBD, which comprises of 1–193 residues, and the chimeric forms of Siah1 and...

Ngày tải lên: 23/09/2015, 14:47

... protein of interest of a specific band of the correct molecular weight and second, competition with increasing amount of soluble antigen Serum from two different rabbits, labeled rabbit 5956 and ... N-terminal of εSG, the PCR product containing the Igκ , FLAG and a small portion of ε-SG ECD was mixed together with the PCR products of the deletions and another round of PCR was performed using P14 and ... members of WH laboratory for their invaluable help, co-operation and advice, Rong Li for mass spectrometric services, IMCB DNA sequencing facility and all staff of the Institute of Molecular and...

Ngày tải lên: 05/10/2015, 21:24

... Purification of HYDBp and HYDJs tot, total proteins; ppt, precipitate fraction; sup, supernatant fraction; puri, purified proteins; M, molecular weight standards For the molecular weight standards, the bands ... SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively On the basis of the homology model, the SGLs of HYDJs and HYDBp (PDB accession number 1NFG) were superimposed and compared ... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is...

Ngày tải lên: 18/02/2014, 08:20

... reaction products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE As shown in Fig 4, 9-HODE is the main product of the hazelnut ... in the middle of July and are harvested at the end of August The maturation stages considered in this work were identified on the basis of seed weight Stage I (harvested at the end of May 2002) ... part of the world production adsorbed by the pastry industry Hazelnut seeds contain reasonable levels of linoleic and linolenic acid (about 10 and 0.2%, respectively, of the total fatty acids) and...

Ngày tải lên: 21/02/2014, 00:20

... organisation emerges in embryos Although we often have very good descriptions of how a particular organ forms and of the nature of the participating cells and molecular constituents, it is in relatively ... (e.g Raff& Kaufman, 1983, p.5) It is true that our understanding of both the genomic and the molecular basis of cell behaviour is limited and inadequate, but this truth is, in my view, thoroughly ... (was he so wrong?) and hence within the egg At this point, reasonable scientific study was abandoned by many biologists and wish became the father of thought and the grandfather of observation:...

Ngày tải lên: 05/03/2014, 11:21

... Molecular Basis of Pulmonary Disease R ESPIRATORY M EDICINE Sharon R Rounds, MD, S ERIES E DITOR Molecular Basis of Pulmonary Disease, edited by Francis X McCormack, Ralph J Panos and Bruce ... Department of Internal Medicine, University of Cincinnati School of Medicine and Cincinnati VA Medical Center, Cincinnati, OH, USA Bruce C Trapnell, MD Department of Pediatrics and Department of Internal ... clinician and a basic scientist who are expert in the disease topic and underlying molecular defect, respectively Their charge was to focus on the genetic basis and molecular pathogenesis of disease,...

Ngày tải lên: 05/03/2014, 23:20

... well as in the molecular mechanisms of effector protein secretion This minireview describes the composition of the cagPAI and the properties of both conserved and unique components of the Cag-T4SS, ... phosphorylation of its effector protein CagA into various host cells is a hallmark of CagT4SS activity Despite the molecular characterization of a number of effects in host cells, the exact function of CagA ... potential implications for the current understanding of its mode of action The cagPAI and the Cag type IV secretion apparatus Gene arrangement and variants of the cagPAI The cagPAI, which was originally...

Ngày tải lên: 06/03/2014, 00:21