nature and properties of microorganisms

... reproduction  eg Candida, Cryptococcus RNA viruses: Influenza Nature and Properties of Micro-organisms Laura JV Piddock Viruses  Obligate intracellular parasites  Use host cell for nucleic acid and ... invasiveness of pathogenic bacteria by reducing opsonisation and protecting from phagocytosis.  E.g. Streptococcus pneumoniae in RTI  vaccination: pneumococcal; meningococcal A and C  GLYCOCALYX ... conjugation (transfer of plasmid DNA) Prions  Infectious protein  No DNA or RNA  Normal cellular form of protein vs. pathological form Pathological form found in neural tissue and tonsils PrP-C...

Ngày tải lên: 12/12/2013, 17:15

... shun friends and family and apprehended going to the grocery store where she dreaded the supermarket owner’s greetings and offers of help. Her husband’s business activities included a certain amount of ... similarities and unique responses to culturally defined social demands. Chapter 2 traces the historic evolution of the notion of social phobia and its equivalents (mostly from the end of nineteenth ... minimize strife and the possibility of loss of face in a skirmish they are bound to lose, social phobic individuals prefer to pro- pitiate and appease. They are soft spoken, docile, and mild; not...

Ngày tải lên: 21/09/2012, 10:54

Ngày tải lên: 27/12/2013, 16:54

... permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. The Nature and Scope of Marketing Hunt, Shelby D. Journal of Marketing (pre-1986); Jul ... without permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction ... Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction...

Ngày tải lên: 18/02/2014, 02:20

Ngày tải lên: 19/02/2014, 14:20

... Identi®cation of the sipV(ba) and sipW(ba) gene regions of B. amyloliquefaciens. (A1/B1) PCR reactions with genomic DNA of B. amy- loliquefaciens and the degenerative primers V1/V3 and V2/V3 or W1 and ... PstIandEcoRI in case of sipV or EcoRI-SacIincaseofsipW and ligated into corresponding sites of pUC18 DNA. The ligation mixes were used for PCR with oligo nucleotides Uni1 or Uni2 and pairs of ... 2002 Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens Hoang Ha Chu, Viet Hoang*, Peter Kreutzmann², Brigitte Hofemeister, Michael Melzer and JuÈ rgen Hofemeister Institute...

Ngày tải lên: 21/02/2014, 03:20

... the K m and k cat values of E. coli Fre for riboflavin are reported as 2.5 lm and 52.4 s )1 with NADPH as the electron donor and 1.3 lm and 30.6 s )1 with NADH [13]. For FMN, K m and k cat values of ... twofold axis associated with the dimer passes through the vicinity of the side chains of Pro13, Ile126, Gln133 and Ile163. The corresponding Table 1. Summary of x-ray data collection, phasing and ... the case of A. vinelandii ferric reductase, the enzyme was purified as a mixture of two proteins or a hetero- dimer with molecular masses of 44 600 and 69 000 Da [25]. Although none of the amino...

Ngày tải lên: 07/03/2014, 02:20

... percentage of the maximum activity. Table 4. Properties of tetrathionate hydrolases from some bioleaching microorganisms. ND, not detected. Species Molecular mass (kDa) (number and mass of subunits) Optimal ... detection and enumeration of themophilic biomining microorganisms. Appl. Environ. Microbiol. 60, 3470–3473. 9. Goebel, B.M. & Stackebrandt, E. (1994) Cultural and phylo- genetical analysis of mixed ... decomposition of tetra- thionate was proposed by Steudel et al.[16],Hazeuet al. [17] and Meulenberg et al. [18] in experiments with intact cells of A. ferrooxidans and A. acidophilum.Propertiesof purified...

Ngày tải lên: 07/03/2014, 14:20

... lombricine (30% of that of taurocyam- ine) and glycocyamine (7% of that of taurocyamine). Neither TK catalyzed the phosphorylation of creatine. Comparison of the deduced amino acid sequences of mitochondrial ... 3381–3385. Cytoplasmic and mitochondrial taurocyamine kinases K. Uda et al. 3530 FEBS Journal 272 (2005) 3521–3530 ª 2005 FEBS Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine ... correlations of this relaxed sub- strate specificity and the possibility of cytoplasmic and mitochondrial isoforms of TK, we have amplified two cDNAs coding for Arenicola brasiliensis TKs and cloned...

Ngày tải lên: 07/03/2014, 21:20

... mixture of 0.05 M NaP i buffer, pH 3, and acetonitrile (6 : 1, v/v); separation was performed isocratically, at a flow rate of 1mLÆmin )1 , and the volume of injected samples was 10 lL. The amounts of ... consists actually of a unique enzymatic protein. Molecular mass and pI determination of F 4¢-OMT. The molecular mass of the pure enzyme was calculated both through gel-filtration and PAGE (Fig. 7) ... the method of Baayen and Elgersma [13], with a 500-lLdropofFod conidial suspension at a concentration of 9 · 10 6 conidiaÆmL )1 ;20 additional plants, inoculated with a 500-lL drop of double distilled...

Ngày tải lên: 08/03/2014, 08:20

... lg of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an [a- 32 P]GTP overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0.5 lgofGSTand0.5lgofGST±Rab proteins, ... GST±Rab31 and Fig. 7. Puri®cation and properties o f wild-type and mutant Rab31 and Rab32 expressed a s GST±fusion proteins. Samples of platelet particu- late fraction protein and of puri®ed GST and ... expression and properties of Rab31 and Rab32 New blood platelet Rab proteins Xiankun Bao 1 , Andrea E. Faris 2 , Elliott K. Jang 1 and Richard J. Haslam 1,2 Departments of 1 Pathology and Molecular...

Ngày tải lên: 08/03/2014, 16:20

... The effect of geometrical parameters and material properties of face sheets and core are studied on the face wrinkling of sandwich plates. Comparison of the present results with those of plate ... anisotropic properties of strength and stiffness of masonry in horizontal and vertical direction, tensile and shear stiffness of cracked masonry, • Concrete yielding in compression, opening of cracks ... tension, mechanism of opening and closing of cracks in concrete under dynamic load, tensile and shear stiffness of cracked concrete, • Strain rate effect of the material properties of masonry, reinforced...

Ngày tải lên: 16/03/2014, 22:20

... expression and properties of the recombinant enzyme Y. T. Pan 1 , J. D. Carroll 2 and A. D. Elbein 1 1 Department of Biochemistry and Molecular Biology and 2 Department of Microbiology and Immunology, ... the cloning and expression of the M. tuberculosis tps gene in E.coli,andthe production of active TPS in these cells. The properties of the recombinant enzyme have been determined, and are com- pared ... starting at position 397 of the M. tuber- culosis TPS, and another sequence of about 16 amino acids starting at position 421 of the M. tuberculosis TPS. Isolation and purification of recombinant TPS To...

Ngày tải lên: 17/03/2014, 10:20

... diameters of the rings, respectively) and t = 160–200 nm (t is the thickness of the rings or length of the tubes) nanorings due to the absence of the vortex states and the presence of out-plane and ... spectra of samples S2 (a) and S3 (b). can be exclusively indexed to ␣-Fe 2 O 3 , according to standard data (JCPDS 33-0664). In Fig. 1(b) and (c), the reflection peaks of XRD patterns of S2 and S3, ... think that sample S2 is Fe 3 O 4 and sample S3 is ␥-Fe 2 O 3 . Fig. 3 presents the SEM and TEM images of samples S1, S2 and S3. Fig. 3(a) and (b) show the morphologies of ini- tial synthesized ␣-Fe 2 O 3 nanotubes...

Ngày tải lên: 20/03/2014, 13:06

... the effect of a mutation in this part of the molecule on the struc- ture and properties of human sHsp. Oligomeric structure of HSP22 and its mutants All samples of recombinant HSP22 and its mutants purified ... understand the role of this part of molecule in the structure and func- tion of small heat shock proteins, we mutated two highly conservative resi- dues (K137 and K141) of human HSP22 and investigated ... trypsinolysis of the wild-type HSP22 and its K137E and K137,141E mutants. Kinetics of trypsinolysis of the wild-type HSP22 (A) and its K137E (B) and K137,141E (C) mutants. The time of incubation...

Ngày tải lên: 23/03/2014, 07:20

... Carroll 4 , Irena Pastuszak 1 and Alan D. Elbein 1 1 Department of Biochemistry and Molecular Biology and 4 Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, ... a number of individual papers can be s treaked with the sugar solution and all run at the same time in the same solvent. Standards of trehalose and maltose are applied to the sides of the paper ... presence of 10 or 50 m M glucose. Fig. 7 shows that 10 m M glucose inhibited both the conversion of maltose t o trehalose and trehalose to maltose b y 3 0–50% at 1 and 3 h of incubation, and t his...

Ngày tải lên: 23/03/2014, 13:20

Ngày tải lên: 23/03/2014, 15:20

Ngày tải lên: 23/03/2014, 21:20

Ngày tải lên: 29/03/2014, 20:20

Ngày tải lên: 31/03/2014, 21:21