nature and properties of microorganisms
Tài liệu Nature and Properties of Micro-organisms doc
... reproductioneg Candida, Cryptococcus RNA viruses: Influenza Nature and Properties of Micro-organismsLaura JV Piddock VirusesObligate intracellular parasitesUse host cell for nucleic acid and ... invasiveness of pathogenic bacteria by reducing opsonisation and protecting from phagocytosis. E.g. Streptococcus pneumoniae in RTI vaccination: pneumococcal; meningococcal A and C GLYCOCALYX ... conjugation (transfer of plasmid DNA) Prions Infectious protein No DNA or RNA Normal cellular form of protein vs. pathological form Pathological form found in neural tissue and tonsilsPrP-C...
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Cambridge.University.Press.Fearing.Others.The.Nature.and.Treatment.of.Social.Phobia.Mar.2007.pdf
... shunfriends and family and apprehended going to the grocery store whereshe dreaded the supermarket owner’s greetings and offers of help.Her husband’s business activities included a certain amount of ... similarities and unique responses to culturally defined socialdemands. Chapter 2 traces the historic evolution of the notion of socialphobia and its equivalents (mostly from the end of nineteenth ... minimize strife and the possibility of loss of face in askirmish they are bound to lose, social phobic individuals prefer to pro-pitiate and appease. They are soft spoken, docile, and mild; not...
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Tài liệu The Nature and Scope of Marketing ppt
... permission.Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.The Nature and Scope of MarketingHunt, Shelby D.Journal of Marketing (pre-1986); Jul ... without permission.Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.Reproduced with permission of the copyright owner. Further reproduction ... Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.Reproduced with permission of the copyright owner. Further reproduction...
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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... Identi®cation of the sipV(ba) and sipW(ba) gene regions of B. amyloliquefaciens. (A1/B1) PCR reactions with genomic DNA of B. amy-loliquefaciens and the degenerative primers V1/V3 and V2/V3 or W1 and ... PstIandEcoRI in case of sipV or EcoRI-SacIincaseofsipW and ligated into corresponding sites of pUC18 DNA. The ligation mixes were used for PCR with oligo nucleotides Uni1 or Uni2 and pairs of ... 2002Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciensHoang Ha Chu, Viet Hoang*, Peter Kreutzmann², Brigitte Hofemeister, Michael Melzer and JuÈ rgen HofemeisterInstitute...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... theKm and kcatvalues of E. coli Fre for riboflavin arereported as 2.5 lm and 52.4 s)1with NADPH as theelectron donor and 1.3 lm and 30.6 s)1with NADH[13]. For FMN, Km and kcatvalues of ... twofold axis associated with thedimer passes through the vicinity of the side chains of Pro13, Ile126, Gln133 and Ile163. The correspondingTable 1. Summary of x-ray data collection, phasing and ... the case of A. vinelandii ferric reductase, the enzymewas purified as a mixture of two proteins or a hetero-dimer with molecular masses of 44 600 and 69 000 Da[25]. Although none of the amino...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... percentage of the maximum activity.Table 4. Properties of tetrathionate hydrolases from some bioleaching microorganisms. ND, not detected.SpeciesMolecular mass (kDa)(number and mass of subunits)Optimal ... detection and enumeration of themophilic biomining microorganisms. Appl. Environ. Microbiol.60, 3470–3473.9. Goebel, B.M. & Stackebrandt, E. (1994) Cultural and phylo-genetical analysis of mixed ... decomposition of tetra-thionate was proposed by Steudel et al.[16],Hazeuet al.[17] and Meulenberg et al. [18] in experiments with intactcells of A. ferrooxidans and A. acidophilum.Propertiesofpurified...
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Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase pptx
... lombricine (30% of that of taurocyam-ine) and glycocyamine (7% of that of taurocyamine). Neither TK catalyzedthe phosphorylation of creatine. Comparison of the deduced amino acidsequences of mitochondrial ... 3381–3385.Cytoplasmic and mitochondrial taurocyamine kinases K. Uda et al.3530 FEBS Journal 272 (2005) 3521–3530 ª 2005 FEBSOrigin and properties of cytoplasmic and mitochondrialisoforms of taurocyamine ... correlations of this relaxed sub-strate specificity and the possibility of cytoplasmic and mitochondrial isoforms of TK, we have amplified twocDNAs coding for Arenicola brasiliensis TKs and cloned...
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Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... mixture of 0.05MNaPibuffer, pH 3, and acetonitrile (6 : 1, v/v);separation was performed isocratically, at a flow rate of 1mLÆmin)1, and the volume of injected samples was 10 lL.The amounts of ... consistsactually of a unique enzymatic protein.Molecular mass and pI determination of F 4¢-OMT. Themolecular mass of the pure enzyme was calculated boththrough gel-filtration and PAGE (Fig. 7) ... the method of Baayen and Elgersma [13], with a 500-lLdropofFod conidialsuspension at a concentration of 9 · 106conidiaÆmL)1;20additional plants, inoculated with a 500-lL drop of doubledistilled...
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... lg of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an[a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing40 lg of platelet protein, 0.5 lgofGSTand0.5lgofGST±Rabproteins, ... GST±Rab31 and Fig. 7. Puri®cation and properties o f wild-type and mutant Rab31 and Rab32 expressed a s GST±fusion proteins. Samples of platelet particu-late fraction protein and of puri®ed GST and ... expression and properties of Rab31 and Rab32New blood platelet Rab proteinsXiankun Bao1, Andrea E. Faris2, Elliott K. Jang1 and Richard J. Haslam1,2Departments of 1Pathology and Molecular...
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Mechanics and Properties of Composed Materials and Structures doc
... The effect of geometrical parameters and material properties of facesheets and core are studied on the face wrinkling of sandwich plates. Comparison of the present results with those of plate ... anisotropic properties of strength and stiffness of masonry in horizontal and vertical direction, tensile and shear stiffness of cracked masonry,• Concrete yielding in compression, opening of cracks ... tension,mechanism of opening and closing of cracks in concrete under dynamic load,tensile and shear stiffness of cracked concrete,• Strain rate effect of the material properties of masonry, reinforced...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... expression and properties of the recombinant enzymeY. T. Pan1, J. D. Carroll2 and A. D. Elbein11Department of Biochemistry and Molecular Biology and 2Department of Microbiology and Immunology, ... the cloning and expression of the M. tuberculosis tps gene in E.coli,andtheproduction of active TPS in these cells. The properties of therecombinant enzyme have been determined, and are com-pared ... starting at position 397 of the M. tuber-culosis TPS, and another sequence of about 16 amino acidsstarting at position 421 of the M. tuberculosis TPS.Isolation and purification of recombinant TPSTo...
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preparation and properties of magnetic iron oxide nanotubes
... diameters of the rings, respectively) and t = 160–200 nm (t is the thickness of the rings or length of thetubes) nanorings due to the absence of the vortex states and thepresence of out-plane and ... spectra of samples S2 (a) and S3 (b).can be exclusively indexed to ␣-Fe2O3, according to standarddata (JCPDS 33-0664). In Fig. 1(b) and (c), the reflection peaks of XRD patterns of S2 and S3, ... think that sample S2 is Fe3O4 and sample S3is ␥-Fe2O3.Fig. 3 presents the SEM and TEM images of samples S1,S2 and S3. Fig. 3(a) and (b) show the morphologies of ini-tial synthesized ␣-Fe2O3nanotubes...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
... the effect of a mutation in this part of the molecule on the struc-ture and properties of human sHsp.Oligomeric structure of HSP22 and its mutantsAll samples of recombinant HSP22 and its mutantspurified ... understand the role of this part of molecule in the structure and func-tion of small heat shock proteins, we mutated two highly conservative resi-dues (K137 and K141) of human HSP22 and investigated ... trypsinolysis of the wild-type HSP22 and its K137E and K137,141E mutants. Kinetics of trypsinolysis of the wild-typeHSP22 (A) and its K137E (B) and K137,141E (C) mutants. The time of incubation...
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Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme ppt
... Carroll4,Irena Pastuszak1 and Alan D. Elbein11Department of Biochemistry and Molecular Biology and 4Department of Microbiology and Immunology, University of Arkansas forMedical Sciences, ... a number of individualpapers can be s treaked with the sugar solution and all run atthe same time in the same solvent. Standards of trehalose and maltose are applied to the sides of the paper ... presence of 10 or 50 mMglucose. Fig. 7shows that 10 mMglucose inhibited both the conversion of maltose t o trehalose and trehalose to maltose b y 3 0–50% at1 and 3 h of incubation, and t his...
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