nanotechnology in stem cell biology and technology

... off in MAO A and B (Fig 3A) The SWIRM domain is a motif commonly found in chromatin enzymes and is responsible for protein–protein and DNA–protein interactions [36] The name of this domain derives ... histone H3 tail polyamines polyamines aromatic amines aromatic amines aromatic amines L-amino acids glycine, sarcosine sarcosine gene expression regulation polyamines metabolism vitamin B5 biosynthesis ... during cell division, and LSD1 was shown to be essential in resetting this memory in the germline in order to ensure pluripotency [21] In parallel, a role for LSD1 in gene activation was originally...

Ngày tải lên: 16/03/2014, 02:20

... the cell, uncoats, and the viral RNA is replicated and translated The SARS-CoV U122 protein induces apoptosis in cells SARS-CoV S and N proteins each can activate the cellular AP-1 protein, which ... originating from one SARS-CoV strain infection in one animal species or cell line in the development of a human vaccine The rapid development of an effective SARS-CoV vaccine depends upon continuing ... canine coronavirus (FCoV and CCoV), and human coronavirus 229E (HCoV-229E) Group II includes porcine hemagglutinating encephalomyelitis virus (HEV), murine hepatitis virus (MHV), bovine, equine,...

Ngày tải lên: 19/06/2014, 08:20

... the cell, uncoats, and the viral RNA is replicated and translated The SARS-CoV U122 protein induces apoptosis in cells SARS-CoV S and N proteins each can activate the cellular AP-1 protein, which ... originating from one SARS-CoV strain infection in one animal species or cell line in the development of a human vaccine The rapid development of an effective SARS-CoV vaccine depends upon continuing ... canine coronavirus (FCoV and CCoV), and human coronavirus 229E (HCoV-229E) Group II includes porcine hemagglutinating encephalomyelitis virus (HEV), murine hepatitis virus (MHV), bovine, equine,...

Ngày tải lên: 20/06/2014, 04:20

... Sources of Stem Cells for Tissue Repair Different types of stem cells include embryonic stem (ES) cells, umbilical cord blood stem cells, organ-specific somatic stem cells (e.g., neural stem cells ... pluripotent stem cells from spermatogonia in the adult human testis, providing another strategy for obtaining genetically identical stem cells Umbilical cord blood stem/ progenitor cells are associated ... multipotent stem cells are already somewhat specialized and may be easier to induce into desired cell types These cells could potentially be obtained from the patient and amplified in culture,...

Ngày tải lên: 07/07/2014, 01:20

... types, including ES cells, bone marrow stem cells, endothelial stem cells, and adipose stem cells Bone marrow stem cells in particular have been examined in clinical trials of human ischemic heart ... likely accomplished by cardiac stem cells resident in the heart, and possibly by cells originating in the bone marrow If such cells could be characterized, isolated, and amplified ex vivo, they ... intravenous, and intracoronary injections In experimental myocardial infarction, functional improvements have been achieved after transplantation of a variety of different cell types, including ES cells,...

Ngày tải lên: 07/07/2014, 01:20

... dysfunction after spinal cord injury reflects demyelination, and both ES cells and marrow-derived stem cells are able to facilitate remyelination after experimental spinal cord injury Clinical trials ... bone marrow and adipose stem cells into neural stem cells, and vice versa, has been reported, and clinical trials of such cells have begun for a number of neurologic disorders Clinical trials ... endoderm, and transdifferentiation of the pancreas to liver and vice versa has been observed in certain pathologic conditions Multipotential stem cells also reside within gastric glands and intestinal...

Ngày tải lên: 07/07/2014, 01:20

... research, in vitro fertilization, and prenatal genetic testing From these and other precedents, we learn the importance of understanding and testing fundamental biology in the laboratory setting and in ... Stem Cell Biology San Diego, Elsevier Academic Press, 2006 Mimeault M et al: Stem cells: A revolution in therapeutics-recent advances in stem cell biology and their therapeutic applications in ... Bodine, J Larry Jameson, and Ron McKay to this chapter in the 16th edition Further Readings Committee on the Biological and Biomedical Applications of Stem Cell Research et al: Stem Cells and...

Ngày tải lên: 07/07/2014, 01:20

... to induce pluripotency in adult somatic cells (Takahashi and Yamanaka, 2006), further demonstrating the pivotal role of transcription factors in the maintaining and inducing the pluripotent cell ... brain, forming the central nervous system Following neural tube closure, patterning is important for determining regional cell fate, and this is mediated by signaling between adjacent cells by cellsurface ... morphogenetic protein (BMP) antagonists such as chordin, noggin and cereberus BMP4 is pivotal in neural induction, as it inhibits cells from forming neural tissue (Finley et al., 1999) Therefore, inhibition...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... target 226 lincRNAs expressed in mESCs, and the authors identified 26 lincRNAs that resulted in loss of Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent ... governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation   104  Chapter VII – Long Non-coding RNAs are Indispensable in ... lncRNAs using anti-SUZ12, anti-SOX2 and anti-OCT4, respectively lncRNA_ES1 and lncRNA_ES2 were enriched in the SUZ12 RIP and SOX2 RIP, indicating interaction * and ** indicate p-values of < 0.05 and...

Ngày tải lên: 09/09/2015, 17:54

... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, ... silencing factor, is a transcription factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the ... expressed in the midbrain and prospective dopamine neurons Rmst transcript was detected by in situ hybridization in the mouse embryonic brain (A, B) Rmst as detected in both thr roof plate (RP) and...

Ngày tải lên: 09/09/2015, 17:54

... is a RNAbinding protein and probably binds directly to RMST SOX2 and hnRNPA2B1 may interact via protein-protein interactions The transcription factor SOX2 may then bind to target chromatin regions ... protein-coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS One 6, e28318 Chen, M., Zhang, J., and Manley, J.L (2010) Turning on a fuel switch of cancer: hnRNP proteins ... elucidated in this thesis Data indicated that RMST is abundant in neurons, and it interacts with the SOX2 via the RNA-binding protein hnRNPA2B1 Expression analyzes following perturbations of cellular...

Ngày tải lên: 09/09/2015, 17:55

... signalling pathways maintaining ES cell pluripotency Embryonic stem cells are maintained by a network of extrinsic and intrinsic signals that collectively regulate the properties of pluripotency and ... pathways involving Zic3 The Zic and Gli family proteins physically interact through their zinc finger domains to regulate neural and skeletal patterning117,118, and the Zic proteins are known to bind ... Zic3 and Sox2 co-occupy physical binding sites in mouse ES cells 4.3 Discussion 4.3.1 Zic3 and Sox2 regulate a common set of pathways in ES cells 4.3.2 Zic3 and Sox2 are interacting partners in...

Ngày tải lên: 11/09/2015, 09:02

... L-glutamine, 10mg/mL streptomycin and 10U/mL penicillin The cells were cultured in an incubator at 37°C, 5% CO2 in a humidified environment 2.1.2.1.2 Over-expression of SPHK1 and SPHK2 in CHO Cells ... inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF and SOV) were freshly added each time before using Substrate preparation: 1mM D-erythro-sphingosine was prepared by mixing 20μl sphingosine (50mM) ... lymphoma cell line The cells were cultured in RPMI 1640 supplemented with 10% FBS (GIBCO, Invitrogen Singapore), 2mM glutamine, 10U/ml penicillin and 10mg/ml streptomycin at 37C, 5% carbon dioxide in...

Ngày tải lên: 11/09/2015, 16:06

... streptomycin and 10U/ml penicillin Cells were cultured in an incubator at 37°C, 5% CO2 in a water-saturated environment 3.1.1.3 Stem Cells Seeding Density Unless otherwise stated, stem cell seeding ... Penicillin and Streptomycin DMEM+10% FBS +1% Penicillin and Streptomycin Supplements N/A 0.5mM isobutyl-methylxanthine, 1μM dexamethasone, 10μM insulin, 200μM indomethacin, 1% Penicillin and Streptomycin ... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization...

Ngày tải lên: 11/09/2015, 16:06

... would be convincing that the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally ... cultured in 6-well plates were washed twice with 1x PBS and starved in serum-free DMEM for half an hour 1μM S1P was then added into the wells and incubated at 37°C for 5min, 10min, 30min, and 60min ... S1P in human BM- and AD- MSCs proliferation proliferation, as well as maintaining their stemness However, human adult stem cells, such as MSCs, are very different with embryonic stem cells in...

Ngày tải lên: 11/09/2015, 16:06

... the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo work, ... expanded to study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem ... for SPHK inhibitors in adult -stem cell differentiation In the final part of my research project (Chapter 4), the bioactive lipid S1P was studied for its role in promoting human BM- and AD- MSCs...

Ngày tải lên: 11/09/2015, 16:06

... “Charaterization of N,N-dimethyl-Derythro-sphingosine-induced apoptosis and signaling in U937 cells: Independece of sphingosine kinase inhibition.” Prostaglandins & other lipid Mediators 86: 18-25 Kim, ... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25 ... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells." Clin...

Ngày tải lên: 11/09/2015, 16:06

... Therefore, mediating stem cells differentiation, in particular, promoting stem cells differentiation, in a controlled way, would be highly desirable in stem cells research and in their future clinical ... particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human adult stem cell proliferation and differentiation ... S1P in stem cells research, derived from the current understanding on human stem cells and the strategies used for stem cell expansion and differentiation, are discussed below 1.4 HUMAN STEM CELLS...

Ngày tải lên: 11/09/2015, 16:06

... receptor Multiple cloning site Methyl-CpG-binding proteins Mouse embryonic stem cell Midbrain-hindbrain isthmus/boundary MicroRNA MicroRNA MicroRNA-containing RNA-induced silencing complexes MicroRNA ... Seng, Huibin, Yin Loon, Minh, Sandy, Phil and all other past and present members of Bing’s lab, thank you for your friendship, and for making the lab such a stimulating and fun place to work in I ... synthase kinase-3 High content screening Histone deacetylases Human embryonic stem cell Inner cell mass Inhibitor of differentiation In situ hybridization Janus-associated tyrosine kinase Leukemia inhibitory...

Ngày tải lên: 12/09/2015, 08:16