nanotechnology for tissue engineering jean s stephens altus and jennifer l west
Automatic microassembly system for tissue engineering assisted with top view and force control
Ngày tải lên :
30/09/2015, 14:24
... concentrates on the serial microassembly relevant studies The assembly systems can be generally categorized into two methodologies, including master-slave systems and automatic assembly systems (Brussel ... macro world In such cases, teleoperation with scaling function is a solution to such problems, and master-slave system is one example of such systems Master-slave systems make the target s scale to ... detailed description of the above listed forces during the two main phases of the assembly process is shown in Table 3-1 And to realize a successful assembly, the forces listed above should follow...
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Biodegradable Polymers as Scaffolds for Tissue Engineering
Ngày tải lên :
25/10/2013, 08:20
... attachment site for the cells responsible to the tissue regeneration Similar to embryonic development, multiple cells should assemble to a specified form for tissue formation To this end, cells would ... Biodegradable Polymers as Scaffolds for Tissue Engineering medical revolution However, the regeneration of human tissues is currently almost impossible except for a few tissues including blood cells, ... mechanical support will be tempolarily necessary until to the full regeneration of the tissue The tissues requiring such mechanical supports include large tubular tissues such as large-calibered blood...
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CULTURE OF CELLS FOR TISSUE ENGINEERING potx
Ngày tải lên :
22/03/2014, 15:20
... cytokeratins for epithelial cells, vimentin for mesodermal cells such as fibroblasts, endothelium, and myoblasts, desmin for myocytes, neurofilament protein for neuronal and some neuroendocrine cells, and ... Cells for Tissue Engineering is a new volume in the John Wiley series Culture of Specialized Cells, with focus on procedures for obtaining, manipulating, and using cell sources for tissue engineering ... and glial fibrillary acidic protein for astrocytes Some cell surface markers are also lineage specific, for example, EMA in epithelial cells, A2B5 in glial cells, PECAM-1 in endothelial cells, and...
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báo cáo hóa học:" Gelatin-layered and multi-sized porous beta-tricalcium phosphate for tissue engineering scaffold" pptx
Ngày tải lên :
21/06/2014, 17:20
... properties were measured by cell proliferation and differentiation The mouse osteoblast cell, MC3T3-E1 cell, (ATCC, Rockville, MD, U .S. A.) was used for in vitro tests The cells (1 × 105 cells/100 l) ... properties of the β-TCP scaffold Figure 5a shows the cell viability results of the scaffolds following 1, 3, 7, and 14 days of culturing that was measured using the CCK-8 assay The cells on the scaffolds ... biological evaluation and performed the statistical analysis KMK participated in the biological evaluation YKL conducted the design and analysis of all experiments as a corresponding author All...
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Applying side effects of anti fibrotic compounds to promote neovascularization for tissue engineering
Ngày tải lên :
11/09/2015, 14:29
... endothelial cells and myoblasts to engineer vascularized skeletal muscle tissue 12 in vitro Perfusion, vascularization and survival of the muscle tissue constructs after transplantation was improved ... factors or supplements apart from the test substances Trypsin-EDTA (Invitrogen, Carlsbad, CA, USA or Lonza, Walkersville, MD, USA) was used to dissociate cells from the culture flasks for cell seeding ... assembled into elliptical clusters (two such elliptical clusters marked by yellow rectangles) DAPI nuclear staining indicates the presence of an evenly spread, confluent layer of cells, revealing...
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Applying side effects of anti fibrotic compounds to promote neovascularization for tissue engineering 1
Ngày tải lên :
12/09/2015, 08:18
... applications vi A sequential co-culture using fibroblasts and endothelial cells was modified and optimized for assessment of in vitro pro-angiogenesis In this inducible system, elliptical clusters ... and nutrients to cells in the interior of a tissue construct; while fibrosis may occur as a result of a foreign body reaction to encapsulate the tissue construct with fibrous collagenous tissue ... Increasing the HUVEC density in sequential co-cultures causes an increase in the size of elliptical clusters formed Figure 11 Increasing fibroblast cell density caused formation of capillary like structures...
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Development of chitin based materials for tissue engineering applications
Ngày tải lên :
17/09/2015, 17:20
... of cellulose It swells slightly in basic solvents and does not swell at all in common organic solvents It is found to be soluble only in special solvents such as 5% lithium chloride (LiCl) in N, ... plants use to build cell walls Likewise to these compounds, chitin also contributes strength and protection to the organism Chitin is widely found in animals particularly in the shells of crustaceans, ... assembly of cellulose, chitin and chitosan, JMS Rev Macromol Chem Phys., C37 (2), 199-276, 1997 17 R.H Jose, Chitin and chitosan, Fungal Cell Wall: Structure, synthesis and assembly, CRC Press,...
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Application of rapid prototyping technology to the fabrication of 3d chitosan scaffold for tissue engineering
Ngày tải lên :
30/09/2015, 13:49
... applications in bone cell cultures while soft chitosan scaffolds are more suitable for soft tissues 26 Chapter Materials and Methods 3.1 Materials Used for Tissue Engineering Scaffolds In literature ... numerous developmental and 27 Chapter Materials and Methods physiological functions The pioneers of skin tissue engineering focused on natural bioresorbable collagen lattices as cell matrices Bell ... Selective laser sintering (SLS) SLS employs a CO2 laser beam to selectively sinter polymer or composite (polymer/ceramic, multiphase metal) powders to form material layers The laser beam is directed...
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Engineering aggregates with chemical linkers for tissue engineering application
Ngày tải lên :
05/10/2015, 21:23
... individual cells as building blocks for tissue engineering Traditional way to get cell aggregates was to use cell adhesion molecule, which was uncontrollable and usually takes several days An alternative ... the most widely used method for culturing large numbers of multicellular spheroids was spinner flask culture [114], roller tubes and gyratory shakers were also used somehow successfully People found ... this system, in which the vessel is completely filled with media and there is a semi-permeable membrane to eliminate bubbles This system successfully integrates co-localization of cells, 3D cell-cell...
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Nanofibrous mat for tissue engineering, wound dressing and dermal reconstitution
Ngày tải lên :
26/11/2015, 22:39
... incubated for days, with PCL/gelatin nanofiber scaffolds as control For a separate set of study, HDF cells were also seeded separately onto the PCL/gelatin nanofiber scaffolds as well as tissue culture ... cells per well onto the PCL/gelatin scaffolds and TG-NF constructs Such low densities were used for proliferation studies because there would be more available areas for cells to proliferate, and ... cell adhesion, growth and infiltration This is a successful first step for the nanofiber construct in establishing itself as a suitable three-dimensional scaffold for autogenous fibroblasts population,...
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báo cáo hóa học: " An overview of tissue engineering approaches for management of spinal cord injuries" docx
Ngày tải lên :
19/06/2014, 10:20
... hematopoietic stem cells (HSCs) and marrow stromal cells (also known as mesenchymal stem cells) (MSCs) are more accessible than other cells mentioned in this review, they have attracted much attention as ... Apart from easier handling, this construct possessed a good flexibility and was able to support formation of blood vessels and migration of astrocytes, Schwann cells, and axons BDNF led to the ... Kao LS, Ma HL: In vitro differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20:522-529 131 Bossolasco P, Cova L, Calzarossa C, Rimoldi SG, Borsotti...
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báo cáo khoa học:" Tissue engineering: a challenge of today''''s medicine" potx
Ngày tải lên :
11/08/2014, 23:22
... themselves in other fields, thereby loosing the ability to open their minds towards other scientific branches To overcome this problem, the demanding, complex and interdisciplinary aspects of tissue ... will be the most significant development for disseminating the results of biomedical researc h in our lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of ... engineering is a vision of Head & Face Medicine We hope this journal will attract basic researchers and clinicians who are involved in investigating and applying tissue engineering in the head and face...
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báo cáo khoa học:" Gene-enhanced tissue engineering for dental hard tissue regeneration: (2) dentin-pulp and periodontal regeneration" pptx
Ngày tải lên :
11/08/2014, 23:22
... osteoblasts, cementoblasts, fibroblasts, and endothelial cells) These cells must subsequently synthesize and release their specific cellular products in a coordinated and sequential manner to ultimately ... odontogenesis and regenerating dentin [35] Human adult dental pulp contains a population of cells ("dentin pulp stem cells"; DPSCs) with stem cell-like properties such as self-renewal and the ability ... improves their survival under hypoxic conditions Mol Cells 2005, 19:402-407 Goldberg M, Smith AJ: Cells and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering...
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Composite controlled release system of nitric oxide for cardiovascular tissue engineering application
Ngày tải lên :
10/09/2015, 09:08
... RSNO S- Nitrosothiol SBS Simultaneous Biaxial Stretching sGC Soluble Guanylate Cyclase SmGM-2 Smooth Muscle Cell Growth Medium SNAC S- Nitroso-N-Acetylcysteine SNP Sodium Nitroprusside TCP Tissue ... be eliminated by using autologous cells Different combinations of cells such as vSMCs, ECs and FBs, or ECs and FBs have been used to construct cell self-assembled TEBVs [125, 126] The cells were ... monolayer at all times for the tissue homeostasis The growth of vSMCs are controlled by both the growth factors and inhibitors released from blood cells, ECs and fibroblasts (FBs) of the vessel...
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Improving the mechanical and functional performance of extrusion based additive manufactured scaffolds for bone tissue engineering
Ngày tải lên :
10/09/2015, 15:51
... mechanical stability of the scaffolds and also to decrease the solubility in water a long post processing was necessary Collagen scaffolds with complex internal morphology and macroscopic shape has also ... osteogenesis progression is affected by pore size Small pores induce osteochondral formation before osteogenesis, whilst large pores that are well vascularized induce direct osteogenesis Tissue ... cell culture study 45 3.3.5.1 Cell seeding on scaffolds 45 3.3.5.2 Morphology of the cell-scaffolds constructs 45 3.3.5.3 AlamarBlue 46 3.3.6 Results and discussions 47 3.3.6.1 Porosity and dispensing...
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Synthesis and characterization of collagen terpolymer fibers for applications in tissue engineering
Ngày tải lên :
11/09/2015, 09:10
... alloy systems will be explored in the said aspects as follows: (i) Stainless steel The most commonly used stainless steel is 31 6L (ASTM F138, F139), grade This steel composition has less than ... immobilisation of cells on substrates, cell infiltration into scaffolds and electroactive materials for tissue engineering will be also presented Chapter presents the synthesis of the polyelectrolytes ... the success of many tissue engineering applications Tissue engineering scaffolds act as templates for cell remodelling and tissue organization The design and development of scaffolding materials...
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An advanced biohybrid nano microscaffold for tendon ligament tissue engineering
Ngày tải lên :
11/09/2015, 09:11
... development, tissue repair and metastasis Their absence hinders the collagen assembly and results in an excessive number of very small collagen fibrils in tendons that decreases the tendon stiffness • ... important for cellular adhesion via integrins, is also present in tendons, ligaments and other connective tissues It also mediates posttranslational collagen fibril modifications and assembly (68) ... the species and anatomical site of the tissue as well as the mechanical testing protocol and conditions Tendons and ligaments typically possess an initial non-linear toe region of low stiffness...
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Tissue engineering approach for annulus fibrosus regeneration
Ngày tải lên :
11/09/2015, 09:57
... press onto the BMSC cell-sheets and silk 10 scaffolds wrapped around it, thus stretching the assembly circumferentially This would 11 allow us to study the effects on the BMSC cell-sheet and ... Blue analysis of sample wells shows no significant difference in cell numbers between sonicated and unsonicated samples of both BMSCs and fibroblasts at weeks post confluence *, p>0.05; (b) Sonication ... microscopy results for cell-sheet thickness at weekly time points 4.1.3c Phase contrast (a, b) and SEM (c, d) images of week old cell-sheets showing very dense mesh-like structures (a) scale bars = 1mm;...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
Ngày tải lên :
11/09/2015, 09:57
... Challenges related to the cellular component of tissue engineering include cell sourcing, as well as controlling expansion and differentiation so as to yield a safe, scalable and functional cell source ... source for cartilage repair Chondrocytes, fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue engineering ... represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee et al., 2006) Adult MSCs isolated...
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