nadh oxidation by peroxynitrite

... of the NADH- oxidase by ADP-ribose The initial rates of NADH oxidation (V0) were measured as described in Materials and methods in the presence of: (curve 1), (curve 2), (curve 3), and lM NADH (curve ... reason for that is the dinucleotide oxidation in the NADH- oxidase reaction At a certain NADH concentration the rate of NAD+ reduction becomes equal to that of NADH oxidation and the steady state is ... for the mitochondrial Ó FEBS 2002 NADH oxidation and NAD reduction catalysed by SPP (Eur J Biochem 269) 4023 Fig The effect of rotenone on the time-course of NADH oxidation (A) and succinate-supported...

Ngày tải lên: 23/03/2014, 21:21

... induction of lipid accumulation in the cells by the particles is enhanced when they are protected from oxidation, we investigated the mechanism of this effect by comparing the influence of CRLPs and ... Biochem 271) CRLPs and cell samples was determined by enzymatic analysis using kits supplied by Sigma Cholesteryl ester levels were calculated by subtracting the values for cholesterol from those ... accumulation without prior oxidation of the particles (Table 3) When probucol was incorporated into the CRLPs, however, this effect was enhanced by more than twofold, and this was caused by an increase in...

Ngày tải lên: 16/03/2014, 16:20

... Oxidation of agmatine by P sativum copper amine oxidase was investigated spectrophotometrically by following the formation of a pink adduct (e515nm ¼ 2.6 · 104 M)1Æcm)1), as a result of the oxidation ... suppressed by a 2-s presaturation before the observation pulse The duration of the pulse corresponding to a flip angle of 90° was 7.4 ls The spin system of the agmatine oxidation product was assigned by ... (B) oxidation catalyzed by P sativum copper amine oxidase, at pD 7.4 and 25.0 °C Acquisition parameters: scans, flip angle 45°, relaxation delay s The residual water signal was suppressed by presaturation...

Ngày tải lên: 17/03/2014, 23:20

... were no significant differences in the amounts of NADH when using bare and nPt-adsorbed GC electrodes Figure Temporal change of NADH concentration (CNADH) in the absence or presence of nPt NAD+ (1 ... generation of NADH, various flat and bulky disk electrodes including platinum were tested as the working electrode for NADH generation in the presence of M A very small amount of NADH was generated ... of NADH gener- Figure a) Concentration of NADH generated on various working electrodes in a solution of M and NAD+, stirred at 340 rpm, at À0.8 V for h b) Temporal change of concentration of NADH...

Ngày tải lên: 03/05/2014, 17:44

... methane oxidation In contrast, understanding of the process of anaerobic methane oxidation is in its infancy Geochemical evidence points strongly towards a coupling of anaerobic methane oxidation ... in anaerobic methane oxidation The protein contains a variant of F430, a cofactor used by the classical MCR, but the two cofactors differ in molecular weight as determined by mass spectrometry ... pathways by which formaldehyde can be oxidized to CO2 (Figure 2b); all the enzymes in this pathway were identified [2], pointing to the importance of this pathway, as suggested previously by enzyme-activity...

Ngày tải lên: 14/08/2014, 14:21

... at À104 mV This result suggests that the oxidation of NADH is catalyzed by PPy on the surface of the electrode Conclusions 3.4 Electrocatalysis of NADH oxidation This investigation is to reveal ... behavior indicates the electrocatalytic oxidation of NADH by the PANI film (Bartlett et al., 1997) These results can be understood by noting that NADH is oxidized to NAD+ at the working electrode ... presence of NADH (CV 2) indicates a quasi-reversible electrode process when compared to CV in the absence of NADH (CV 1) which clearly shows that the P4VP film is active for NADH oxidation Fig...

Ngày tải lên: 02/09/2015, 13:45

... by Novo Nordisk Biotech and purified by ion-exchange chromatography on Q-Sepharose by elution with phosphate buffer [5,20], and an activity of 10 000 UÆmL)1 determined spectrophotometrically by ... mediation by TEMPO Tetrahedron Lett 42, 7551– 7553 18 Fritz-Langhals, E & Kunath, B (1998) Synthesis of aromatic aldehydes by laccase-mediator assisted oxidation Tetrahedron Lett 39, 5955–5956 Oxidation ... dioxygen on the phenoxy radical [12] The spectra in Fig refer to oxidations carried out in the mixed Fig Oxidation of substrates 1–5 by O2-laccase and O2-laccase-HPI Phenol coupling with loss of...

Ngày tải lên: 21/02/2014, 01:21

... cadaverine (CAD) and the Substrate oxidation by amine oxidase CAD HEX pH 10 Fig Effect of pH on kinetic parameters of the CAD (d) and HEX ( ) reductive half-reaction catalyzed by PSAO (A) kc, (B) log(1 ... precisely regulated by specific interactions In particular, the binding of HEX to the active site is controlled by hydrophobic contacts, whereas the approach of CAD is facilitated by electrostatic ... catalytic cycle Substrate oxidation by amine oxidase 12 ΔH# 10 –TΔS# 6 pH 10 Fig Effect of pH on heats of activation and activation entropy for oxidative deamination catalyzed by PSAO DH* and TDS*...

Ngày tải lên: 06/03/2014, 00:21

... the NADP+ oxidation reaction is highly regiospecific Kinetics of NADPO formation as catalyzed by FprA and AdR Figure 4A shows the time courses of NADP+ oxidation to NADPO catalyzed by FprA or ... NADP+ oxidation to NADPO catalyzed by FprA, an NADPO assay method was required After testing different analytical chromatographic procedures, we found the ion exchange method described by Orr ... to FprA concentration, showing that the NADP+ oxidation, although very slow, was strictly enzyme-dependent The NADP+ oxidation reaction catalyzed by FprA was much slower (0.14 h)1) than that of...

Ngày tải lên: 16/03/2014, 11:20

... steps in oxidations catalyzed by rabbit cytochrome P450 1A2 Biochemistry 43, 10775–10788 19 Yun C-H, Kim K-H, Calcutt MW & Guengerich FP (2005) Kinetic analysis of oxidation of coumarins by human ... of multiple products by different kinetic mechanisms of cytochrome P450 enzymes Biochemistry 33, 2927–2937 12 Ortiz de Montellano PR & De Voss JJ (2005) Substrate oxidation by cytochrome P450 enzymes ... intrinsic kinetic deuterium isotope effects for Fig Oxidation reactions with coumarins catalyzed by P450s 2224 7-OEt coumarin O-deethylation by some rat P450 enzymes (today known as P450s 2B1...

Ngày tải lên: 16/03/2014, 13:20

... The oxidation of N-hydroxyguanidine and of NOHA by H2O2 catalysed by MP8 clearly involves an active species derived from the haem of MP8 as metal-free MP8 was inactive It also involves high oxidation ... chemical systems known to proceed by mono-electronic transfers and with peroxidase-type reactions catalysed by MP8 It probably involves initial oxidation of MP8FeIII by H2O2 to MP8 compound I (MP8+FeIV ... oxidation of N-hydroxyguanidine by H2O2 in the presence of MP8 Further one-electron oxidations of the intermediate iminoxy-radical (or its mesomeric form, the nitroso-C-centered radical 5), by...

Ngày tải lên: 17/03/2014, 09:20

... nm/s The specimen were glued onto the holder by silver paste, and then placed in a SEIKO’s SPI3700/SPA300 AFM, which provides anodic oxidation functions By scanning the cantilever with the applied ... resistance can be expressed by the formula as Fig Scheme of the fabrication of TiO2 nanowire DR ¼ rs d  L L À rs d  dða0 À ntÞ d Á a0 where a0 is the width before oxidation, L is the length, ... temperature Conclusion Fig Mechanism of AFM anode oxidation Adopting AFM fabrication technique, a 70-nm wide TiO2 nanowire has been fabricated on Ti film By monitoring ARTICLE IN PRESS 1336 Z Li et...

Ngày tải lên: 20/03/2014, 13:06

... oxynitrite-mediated oxidation of HPX (Table 2), reflects oxidation of the heme–Fe atom by CO3– rather than conformational transition(s) depending on the nitration of Tyr and Trp residues by NO2 As observed ... reported for peroxynitrite- mediated oxidation of human Ngb(II)–NO [32] Under the experimental conditions, kinetic and spectroscopic properties of HPX–heme(II)–NO were unaffected by decomposed peroxynitrite ... was kept under helium Peroxynitrite was prepared from KO2 and NO and from HNO2 and H2O2, under anaerobic conditions Peroxynitrite was purified by freeze fractionation The peroxynitrite concentration...

Ngày tải lên: 23/03/2014, 10:20

... from b -oxidation To examine whether adipophilin may increase cellular TGs by inhibition of fatty acid oxidation, experiments were performed with AICAR, which stimulates fatty acid oxidation by activation ... data suggest that stimulation of fatty acid oxidation by AICAR is dominant over the inhibition of fatty acid oxidation by adipophilin The mechanism by which this occurs is unknown, but may be ... with palmitate only This indicates that fatty acid oxidation is an ongoing process in THP-1 macrophages; inhibition of fatty acid oxidation by bromopalmitate results in elevated cellular TG content...

Ngày tải lên: 23/03/2014, 10:21

... supported by the observed regeneration of phenol red, following its oxidation at the electrode, by the presence of 4-methoxybenzyl alcohol (cf Figure 6)] and (c) this phenoxy radical, as suggested by ... methods Laccase Laccase from P pinsitus was kindly donated by Novo Nordisk Biotech; it was purified by ion-exchange chromatography on Q-Sepharose by elution with phosphate buffer; laccase fractions with ... oxidized by laccase in the absence of a mediator, whereas the latter yielded only a trace of di-t-butylbenzoquinone (predominantly the p-benzoquinone isomer See Fig for product identification by MS) Oxidation...

Ngày tải lên: 23/03/2014, 21:20

... FAD The specic activity of NADH oxidase is % 1.9 Uặmg)1 in mM phosphate buffer, pH 7.0 DH ẳ Ea RT   kcat T DS ẳ ỵ C2 T ln T R m ẳ Vmax DG ẳ DH TDS NADH NADH ỵ KNADH m;app 1ị where, Km, ... phosphate buffer containing 0.12 mM FAD and 120 nM NADH oxidase Reactions were started by the addition of NADH to achieve a nal concentration of 0.18 mM NADH Initial velocities were measured in the ... The reaction was started by the addition of 180 lM NADH To determine the Km value the concentration of NADH was varied in the range 5200 lM It is not possible to use NADH at higher concentrations...

Ngày tải lên: 30/03/2014, 13:20

... superoxide or NADH in aerobically prepared crude extracts and membranes resulted in increased Q reductase activities, but the uncoupling of NADH oxidation from Q reduction could not be prevented by these ... described by Tokuda & Unemoto, with NADH oxidation and Q reduction activities of 2.45 and 0.99 lmolÆmin)1Æmg)1, respectively [15] Inhibition and uncoupling of the Na+-NQR activity by dioxygen ... exclusion of air ND, not determined Conditions of cell rupture NADH oxidation (lmolÆmin)1Æmg)1) Q1 reduction (lmolÆmin)1Æmg)1) Ratio of NADH oxidation/ Q1 reduction activity + + + – 1.7 3.2 (4.7)a 1.6...

Ngày tải lên: 31/03/2014, 21:21

... SiO2 layers with embedded metal and metal silicide NCs by the combination of Si:Me mixture by PLD at room temperature and its thermal oxidation By means of this fabrication technique, it is possible ... http://www.nanoscalereslett.com/content/6/1/148 20 Page of SiPt SiAu 0 oxidation h, 640 oxidation h, 640 18 28 26 16 CSiO , pF oxidation h, 640 C oxidation h, 640 C 30 24 22 14 20 18 12 16 10 14 12 10 6 -5 ... for SiO2:NCAu based structures prepared by dry oxidation On the contrary, in the case of SiO2:NC-PtSi, the maximal flatband voltage shift was U = 1.2 V By increasing V g sweep up to V, a gradual...

Ngày tải lên: 21/06/2014, 05:20

... exceptional cases: i) in the case of oxidation of 5, the reaction temperature provided by microwave or conventional heating increased the yield of 5b, ii) in case of the oxidation of 4, the assistance ... Na2SO4) After removal of the solvent by rotational evaporation, the purity of crude product was analysed by GC/MS The pure ketone was isolated from the crude product by flash column chromatography ... hexane:dichloromethane and identified by NMR spectroscopy, and IR spectroscopy 4.5 .Oxidation of alkylarenes into corresponding ketones under solvent-free reaction conditions assisted by ultrasound irradiation...

Ngày tải lên: 22/07/2014, 03:20

... induced by activated chondrocytes is mediated by lipid peroxidation reaction, glucosamine decreases collagen degradation by inhibiting advanced lipoxidation reaction and thus prevents the oxidation ... degradation by glucosamine was not mediated by inhibiting the chondrocyte lipid peroxidation process but by Page of 10 (page number not for citation purposes) inhibiting advanced lipoxidation reactions ... formation by activated chondrocytes and lipoprotein oxidation We monitored conjugated diene formation as an indicator of lipid peroxidation in activated chondrocytes and purified lipoprotein oxidation...

Ngày tải lên: 09/08/2014, 10:20