nad p h quinone oxidoreductase 1 expression hydrogen peroxide levels and growth phase in hela cells
Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
Ngày tải lên :
30/03/2014, 03:20
... (c) pHPI -15 07 pHPI -15 07 kDa 24 17 pHPI -14 94 kDa Control MG132 Lactacystin DMSO MG132 pHPI -15 07 pHPI -14 94 Control pHPI -14 95 MG132 pHPI -14 95 (a) B 20 14 pHPI -15 79 +MG132 pHPI -15 79 pHPI -14 96 +MG132 ... protein 17 kDa 45 36 anti-core +1 29 24 6 (pHPI -12 03) pNS4B GFP (pA-EUA2) Detergent phase pGFP (pHPI -14 95) queous phase Control pcore +1/ S–myc Control (pA-EUA2) pGFP (pHPI -14 95) kDa (pHPI -14 95) ... of adenine (A) at core codons 8 11 myc myc myc – 342–825 pHPI -14 94 (pcDNA3 .1( –) ⁄ Myc-His) pHPI -15 07 (pcDNA3 .1( –) ⁄ Myc-His) pHPI -14 95 (pcDNA3 .1( –) ⁄ Myc-His) pHPI -17 05 (pcDNA3 .1( –) ⁄ Myc-His)...
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Sphingosine kinase 1 regulates the expression of proinflammatory cytokines and nitric oxide in activated microglia
Ngày tải lên :
16/10/2015, 15:38
... synthases or, sphingosine can be phosphorylated to sphingosine -1- phosphate (S 1P) by sphingosine kinases (Hannun and Obeid, 2008, Olivera, et al., 19 98) S 1P is dephosphorylated by sphingosine-1phosphate ... of SphK2 is cell type specific (Okada, et al., 2005, Sankala, et al., 2007) Both of the kinases phosphorylate erythro-sphingosine (Sphingosine), dihydrosphingosine and phytosphingosine, which are ... sphingosine-1phosphate phosphatase to form sphingosine The final step in the biosynthesis is the irreversible cleavage of S 1P into ethanolamine phosphate and hexadecenal by S 1P lyase (Snider, et al., 2 010 )...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
Ngày tải lên :
03/11/2012, 11:44
... (PKN1 and DIAPH1) and CPI -17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor Biol Reprod 2007; 76: 9 71- 82 44 Madigan MC, Kingsley ... treatment with pAnt and HIV-Tat carrier molecules, in which the pAnt treated probe shows increased gene expression compared to the HIV -1 Tat probe The PKN1 protein kinase N1 is a transcript variant ... intrinsic pharmacological and physiological properties In our lab, gene expression experiments with different transport peptides (TP) in HeLa cells stably expressing EGFP indicated an increase of EGFP...
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Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
Ngày tải lên :
16/02/2014, 15:20
... 10 18. 51 b17 ++ 12 55.5 b 21 ++ 953.5 b16 ++ 877.5 y7 0.5 10 92 .1 b18 ++ 9 61. 9 b16 ++ 418 .1 y3 pY 12 30.6 y20 ++ 290 .1 y2 11 91. 1 y10 13 13 .1 y 21 ++- 97 17 22.7 b14 25 kDa 18 51. 0 b15 400 B 16 06.8 b13 - ... y1 10 49.9 y18++ 11 92.62 y10 13 55.69 y 11 1426.69 y12 678.35 y 11+ + 211 .16 b2 985.52 y17++ 15 39.86 y13 25 kDa 200 400 600 16 99.89 y14 10 77.60 y9 800 10 00 12 00 14 00 16 00 18 14.83 y15 18 00 m/z Fig Detection ... its serine ⁄ threonine kinase activity [13 ,15 ,37] and binding to actin [12 ], YpkA has been shown to interact with small GTPases and inhibit nucleotide exchange in Rac1 and RhoA, mimicking the guanidine...
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Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc
Ngày tải lên :
18/02/2014, 12:20
... weight HSPs such as HSP10, HSP20, HSP27, HSP32, and HSP40 [18 ] The predominant HSPs, HSP70 and HSP27, have been well studied HSP70 and HSP27 have been found both in the cytoplasm and in the nucleus ... of input HSP 27 30 0.8 HSP70 20 10 eIF4G 1. 0 HSP27 B HSP70 E 50 HSP 27 (2.2) 1. 0 1. 3 HSP70 HS Re12 h Re24 h 40 (2.5) % of input (0.6) 30 20 10 PABP 1. 0 0.7 1. 9 1. 8 HSP27 C HSP70 HSP 27 HSP70 ... translation [8 ,10 ] Both PAIP1 and PAIP2 have similar PABP1-binding motifs, and compete for binding with PABP1 [10 ] As such, PAIP2 works as an inhibitor of mRNA translation by competing with PAIP1 Eukaryotic...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... 80 10 0 10 1 10 0 10 1 10 2 10 0 10 3 10 0 10 4 +Fe 3.2% 10 1 10 2 10 3 10 0 10 1 10 2 10 3 Counts 20 40 60 80 10 0 10 4 10 0 10 1 10 2 10 4 CD1d42 10 0 10 3 10 4 10 0 –Fe 12 .2% 10 1 10 2 10 3 10 4 MHC-II –Fe 10 1 10 2 10 3 10 4 ... +saponin None B Counts 20 40 60 80 10 0 TfR 10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 – saponin +saponin +saponin 20 40 60 80 10 0 Counts – saponin C Counts 20 40 60 80 10 0 Negative 10 0 10 1 10 2 10 3 ... expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins of HepG2...
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Báo cáo khoa học: Mammalian 105 kDa heat shock family proteins suppress hydrogen peroxide-induced apoptosis through a p38 MAPK-dependent mitochondrial pathway in HeLa cells potx
Ngày tải lên :
16/03/2014, 06:20
... Hsp105a and HeLa- tet ⁄ Hsp105b) in which overexpression of mouse Hsp105a or Hsp105b was induced by removing DOX from the culture medium In HeLa- tet ⁄ Hsp105a cells, the level of Hsp105a was increased ... experiments, and typical blots are shown in HeLa cells treated with H2 O2 On the other hand, we observed previously that Hsp105a and Hsp105b suppress staurosporine-induced apoptosis by inhibiting ... MAPK) pathway but not the JNK pathway Results Hsp105a and Hsp105b suppress apoptosis induced by H2 O2 but not by heat shock in HeLa cells Previously, we have established human HeLa cell lines (HeLa- tet...
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Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx
Ngày tải lên :
16/03/2014, 11:20
... Oligonucleotide probe sequences complementary to mouse and rat Adh1, Adh3, Adh4 and Aldh1 Probe Gene Species Sequence 5’- to 3’ Adh1 Adh1 -1 Adh1-2 Adh3 Adh3 Adh4 Adh4 -1 Adh4-2 Adh4-3 Aldh1 Random Adh1 (class ... identified in humans ADH1 is abundant in liver where it participates in the oxidation of ingested ethanol [11 ] Human ADH1 is the only class consisting of isoenzymes, namely ADH1A, ADH1B and ADH1C, although ... evolution and chromosomal mapping Pharmacogenetics 9, 4 21 434 Perozich J, Nicholas H, Wang BC, Lindahl R & Hempel J (19 99) Relationships within the aldehyde dehydrogenase extended family Protein Sci...
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Báo cáo khoa học: Gene expression waves Cell cycle independent collective dynamics in cultured cells doc
Ngày tải lên :
30/03/2014, 08:20
... cycle as the driving force of the observed pattern, showing that the pc1small–pc1whole correlation is driven by their mutual correlation with pc1rand, thus indicating an aspecific (from the point of ... mode of the whole set of 275 ribogenesis genes (pc1whole) and of the subset of 14 genes (pc1small) are compared in Fig 1( A), which shows the scores of the first principal components for both data ... pcribo3 and pcrand1–pcrand3), respectively The first component explained 54.5% and 55.4% of the total variance in RIBO and RAND sets, respectively, confirming the alpha-factor results with respect...
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Báo cáo y học: "κ Increased AP-1 and NF-κB activation and recruitment with the β combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocyte" ppt
Ngày tải lên :
09/08/2014, 01:23
... with rheumatoid arthritis Clin Exp Immunol 2002, 12 7:539-546 Chabaud M, Page G, Miossec P: Enhancing effect of IL -1, IL -17 , and TNF-alpha on macrophage inflammatory protein-3alpha production in ... through mechanisms involving AP -1, NF-κB and Egr -1 pathways R196 Limited information is available on the effect of IL -17 on AP -1 Here, IL -17 alone had a weak effect compared with that of IL -1 ... 4% paraformadehyde and washed intensively (3 × min) with × PBS Cells were then permeabilized for in 0 .1% Triton X -10 0 /1 × PBS, followed by three washings Nonspecific binding was abolished by incubation...
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Báo cáo y học: "Expression of 5-lipoxygenase and 15-lipoxygenase in rheumatoid arthritis synovium and effects of intraarticular glucocorticoids" docx
Ngày tải lên :
09/08/2014, 14:21
... The 15 LO -1 enzyme has proinflammatory actions, with high levels of 15 -HETE reported in sputum of asthmatic patients along with increased macrophage 15 -LO -1 mRNA expression [11 ] 15 LO -1 expression ... 15 -Lipoxygenase -1 CD163+ macrophages, CD 31+ endothelial cells and prolyl 4-hydrolase-positive fibroblast cells 15 -Lipoxygenase -1 express 15 -lipoxygenase -1 (15 -LO -1) in rheumatoid arthritis synovium Photomicrographs ... manuscript MK participated in acquisition and interpretation of data, and in writing the manuscript AIC provided the patient biopsies and their clinical data and participated in writing the manuscript...
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báo cáo khoa học: " Regulation of hypoxia inducible factor-1a expression by the alteration of redox status in HepG2 cells" pdf
Ngày tải lên :
10/08/2014, 10:21
... All authors read an approved the final draft Competing interests The authors declare that they have no competing interests Received: March 2 011 Accepted: 19 May 2 011 Published: 19 May 2 011 References ... Research 2 011 , 30: 61 http://www.jeccr.com/content/30 /1/ 61 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 conditions Its stabilization by hypoxia depends on redox-induced changes J Biol Chem 19 97, ... Jaakkola P, Chang GW, Clifford SC, Maher ER, Pugh CW, Ratcliffe PJ, Maxwell PH: Hypoxia inducible factoralpha binding and ubiquitylation by the von Hippel-Lindau tumor suppressor protein J Biol Chem...
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Báo cáo y học: "Suppressing miRNA-15a/-16 expression by interleukin-6 enhances drug-resistance in myeloma cells" ppt
Ngày tải lên :
10/08/2014, 21:23
... expression in U266 and NCI -H9 29 cells in a time- and dose- dependent pattern enhanced the survival of the MM cells and protected them from drug-induced apoptosis by suppressing miRNA -15 a/ -16 expression ... manuscript and gave final approval of the version to be submitted All authors have read and approved the final manuscript Conflicts of Interests The authors declare that they have no competing interests ... apoptosis and miRNA -15 a/ -16 expression in MM cells (A) MM-BMSCs inhibited apoptosis of MM cells induced by cytotoxic agent (B) Stem-loop RT-PCR assay showed that miRNA -15 a /16 expression in MM cells...
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báo cáo khoa học: " Identification of drought-response genes and a study of their expression during sucrose accumulation and water deficit in sugarcane culms" potx
Ngày tải lên :
11/08/2014, 11:21
... genes including those involved in the synthesis of osmoprotectants, may have a role in protecting the cells, and maintain pressure potential by providing compatible solutes in the cytoplasmic compartment ... co-regulates the expression of asparagine synthetase and proline dehydrogenase 2, linking sugar content with asparagine and proline metabolism [26] However, AtbZIP 11 is in the sequence group S of bZIP transcription ... Biology 2 011 , 11 :12 http://www.biomedcentral.com /14 71- 2229 /11 /12 April 2005 Conditions inside the glasshouse, which had ambient lighting, were set at 31 C/22°C for day and night temperatures, respectively...
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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
Ngày tải lên :
12/08/2014, 04:21
... animals expressing the human receptors for HIV -1, including mice [10 ,11 ], rats [12 ], and rabbits [13 ,14 ] The outbred transgenic rat model, expressing hCD4 and CCR5 on lymphocytes, macrophages, and microglia, ... the chimera sequence between the LTR of HIV -1 and the LINE -1 repetitive sequence of the cotton rat References 10 11 12 13 14 15 Nath BM, Schumann KE, Boyer JD: The chimpanzee and other non-human-primate ... additional 16 h The next day cells were infected with HIV -1 MN virus (1. 6 × 10 4 TCID50) or with a mock viral preparation Cells were incubated with the inocula for h, washed with PBS, and incubated with...
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Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
Ngày tải lên :
12/08/2014, 04:21
... animals expressing the human receptors for HIV -1, including mice [10 ,11 ], rats [12 ], and rabbits [13 ,14 ] The outbred transgenic rat model, expressing hCD4 and CCR5 on lymphocytes, macrophages, and microglia, ... the chimera sequence between the LTR of HIV -1 and the LINE -1 repetitive sequence of the cotton rat References 10 11 12 13 14 15 Nath BM, Schumann KE, Boyer JD: The chimpanzee and other non-human-primate ... additional 16 h The next day cells were infected with HIV -1 MN virus (1. 6 × 10 4 TCID50) or with a mock viral preparation Cells were incubated with the inocula for h, washed with PBS, and incubated with...
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Báo cáo sinh học: " Exact p-value calculation for heterotypic clusters of regulatory motifs and its application in computational annotation of cis-regulatory modules" doc
Ngày tải lên :
12/08/2014, 17:20
... confirm the accuracy of our algorithm and show in what cases the Poisson approximation [8 ,11 ] cannot be employed In the subsection 'Optimal cutoffs', we apply AHOPRO to choose an appropriate cutoff ... Salvy and Philippe Flajolet for helpful comments and suggestions Thanks to Alexander Favorov for help with the programming Thanks to Tim Barker for correcting the English in the manuscript This ... 0. 013 0.00026 5.8E-06 0. 010 0.0033 0. 012 3.6E-05 4.34E-07 1. 00 1. 01 0.99 0.99 1. 13 1. 10 1. 34 1. 04 7 .10 7 .13 Comparison of p- values calculated for the Markov(0) model by the AHOPRO program with p- values...
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Báo cáo y học: " Analysis of HIV-1 Vpr determinants responsible for cell growth arrest in Saccharomyces cerevisiae" ppt
Ngày tải lên :
13/08/2014, 13:20
... as in fission yeast Schizosaccharomyces pombe (Sc Pombe) [10 14] Involvement of protein phosphatase 2A (PP2A), Wee1, Cdc25C, and 14 -3-3 proteins has also been implicated [8 -12 ,14 ] but the host ... and apoptosis, in mammalian cells or HIV -1 infected T cells [38] Even though the inhibitory effect of these di-Wcontaining peptides correlated with their ability to interact with Vpr in budding ... this question Materials and methods Yeast strain The S cerevisiae yeast strain used in this study was the protease-deficient HP -16 strain (MAT∝ ura3-52 his3 1 leu2 trp1∆63 prb1 -11 22 pep4-3 prc1-407)...
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Báo cáo sinh học: " Effects of recombinant adenovirus-mediated expression of IL-2 and IL-12 in human B lymphoma cells on co-cultured PBMC" pot
Ngày tải lên :
14/08/2014, 19:22
... DF, and ISW participated in the design of the study and its coordination All authors have read and approved this manuscript http://www.gvt-journal.com/content/2 /1/ 15 13 14 15 16 17 18 19 Acknowledgments ... method accomplishing highly efficient adenoviral-mediated gene transfer in lymphoma cells [6] Using this protocol, expression of the wild-type p5 3 tumor-suppressing gene in lymphoma cell lines ... coxsackie-adenovirus receptor and alpha(nu)beta5 integrin in SUDHL -1 cells derived from anaplastic large-cell lymphoma Clin Cancer Res 2000, 6 :18 5 -19 2 Lu PH, Negrin RS: A novel population of expanded human CD3+CD56+...
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The effects of chloride ions on the hydrogen gas generation and TCE degradation in aqueous solutions containing zero valent iron
Ngày tải lên :
13/05/2016, 10:16
... with the assumption that the produced H2 accumulated only in the liquid and gas phase Total moles of hydrogen gas contain the moles of H2 in the aqueous phase and the gas phase The moles of hydrogen ... rate at pH 4.5 in the presence of 65ppm TCE H2 generation rate (mmol.kg-1d -1) 10 0 80 pH 4.8 pH 6.4 pH 4.8 + 65 ppm TCE pH 6.4 + 65 ppm TCE 60 40 20 0 20 40 60 80 10 0 Time (h) Figure Hydrogen ... 4 .1. 3 Hydrogen gas generation in the presence of TCE and chloride ions H2 generation rate (mmol.kg-1d -1) 60 pH 4.8 + 65 ppm TCE pH 6.4 + 65 ppm TCE pH 4.8 + 65 ppm TCE + 20 mM NaCl pH 6.4 + 65 ppm...
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