na uptake and accumulation in roots

... (2007) investigated the effects of pH on short-term uptake of manganese and cadmium into Chlamydomonas reinhardtii, and they concluded that the increase in proton concentration inhibits the metal internalization ... metal internalization flux non-competitively by binding to a binding site other than BL In addition, changes in the conformation of BL caused by this binding of proton result in decreases in the ... cupric ion, and Min+ is the competing cation that inhibits copper toxicity and accumulation In this study, calcium ion and proton were assumed to be the only competing cations WHAM VI (The Windermere...

Ngày tải lên: 05/09/2013, 10:15

... shown in Fig In the anaerobic phase, PO4-P concentration increased to a certain value and remained constant in each run In Run1-1, PO4-P concentration in the anaerobic phase was higher than that in ... in activated sludge process with coagulant addition, Journal of Environmental Systems and Engineering, 748(VII-29), 43-55 Mishima I and Nakajima J (2003c) Coagulants and phosphorus behavior in ... supposed to be influenced by Fe addition which had indirect effects on the biological phosphorus uptake and release during the anaerobic and aerobic phases Therefore, three key points of phosphorus...

Ngày tải lên: 05/09/2013, 10:15

... Daily intake of cadmium and its concentration in the renal cortex Over 100 µg/g of cadmium in the renal cortex was also reported in the 1970s and 1980s in Japan 268 in 1970 and 182 g/day in 1994 ... residents in an area contaminated by irrigation water in China Bull World Health Organ 1995; 73: 359–367 25) Rivai IF, Koyama H, Suzuki S Cadmium content in rice and rice field soil in China, Indonesia, ... trends in cadmium exposure in ordinally inhabitants, especially net cadmium intake by ingestion This will be useful as baseline data for cadmium intake except via smoking or occupational exposure...

Ngày tải lên: 15/03/2014, 23:11

... and Bock, 2002) Cholesterol in the brain may play an important role in synaptic development and plasticity, brain growth, neuron repair and remodeling, and even maintaining the normal function of ... docking, and fusion (Edinger et al., 2003), and inactivation by GAPs (Grosshans et al., 2006; Pfeffer and Aivazian, 2004; Zerial and McBride, 2001) Individual rab proteins are found on distinct ... cleave a variety of intracellular polypeptides, including major structural elements of the cytoplasm and nucleus, components of the DNA repair machinery, and a number of protein kinases, which then...

Ngày tải lên: 14/09/2015, 14:06

... determined by liquid-scintillation counting using Hydrofluor as the scintillant Analytical methods TC (cholesterol + cholesteryl ester) and TG in CRLPs and in cells was determined by enzymatic analysis ... cells and experimental protocol THP-1 monocytes were maintained in suspension in RPMI 1640 culture medium containing 10% fetal bovine serum, 100 UÆmL)1 penicillin, 100 mgÆmL)1 streptomycin and ... receptorbinding region is in an amphipathic a-helix which is part of a four-helix bundle structure in the N-terminal domain, and interaction with lipid is necessary to induce a conformational change...

Ngày tải lên: 16/03/2014, 12:20

... containing 70% trilinolein, 2% cholesterol, 5% cholesteryl ester and 25% phospholipids, in Tricine buffer (20 mM, pH 7.4) containing 0.9% NaCl, followed by density-gradient centrifugation and binding ... cells THP-1 monocytes were maintained in suspension in RPMI1640 containing 10% fetal bovine serum, mM glutamine, 100 UÆmL)1 penicillin, 100 mgÆmL)1 streptomycin and 50 mM 2-mercaptoethanol (culture ... these compounds against cardiovascular disease [11] Dietary lipids, including fats, cholesterol and lipid-soluble antioxidants, are absorbed in the intestine and secreted into lymph in chylomicrons,...

Ngày tải lên: 16/03/2014, 16:20

... was examined by MAR-FISH, and the results are shown in Figs and Lower initial pH conditions increased anaerobic acetate uptake by Candidatus ‘Accumulibacter phosphatis’ in the second and third ... determine the effect of pH on anaerobic acetate uptake by Candidatus ‘Accumulibacter phosphatis’ and Candidatus ‘Competibacter phosphatis’ Within the tested pH range (initial pH from to and final ... around and after the incubation, which originally ranged between and or The effect of pH on the anaerobic uptake of radio-labeled acetate by Candidatus ‘Accumulibacter phosphatis’ and Candidatus...

Ngày tải lên: 05/09/2013, 09:38

... NADPH and MSR AqCbl Without MSR or NADPH With MSR With FMN domain With NADPH and MSR With NADPH and CPR With NADPH and nNOSred MeCbl Without MSR or NADPH With MSR With FMN domain With NADPH and ... of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark ... (light grey) and the C-terminal activation of hMS (grid-barrel) interact with the cobalamin-binding domain For more information on hMS conformational substates, see [5] and [33] cob(II)alamin], an...

Ngày tải lên: 18/02/2014, 08:20

... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins ... without and with iron Thin lines represent background staining with mouse Igs (B) Histograms show background staining with no antibody (thin lines) or with rabbit Igs (thick lines) as first step in ... were counted in each time point (B) For ROS determination growing cells were first incubated with 10 lM of DCFH-DA, harvested and acquired immediately in a FACSCalibur and analyzed using the CELLQUEST...

Ngày tải lên: 19/02/2014, 16:20

... Glutamine incorporation in Caco-2 proteins determining the relative uptake of glutamine; (b) by searching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into ... Desmulin Clathrin heavy chain Cadherin-17 [precursor] Alpha-actinin Endoplasmin [precursor] Heat shock protein HSP 90-beta Villin Stress-70 protein, mitochondrial [precursor] Succinate dehydrogenase ... proteins that play a role in protein folding and processing (protein disulfide-isomerase, protein disulfide-isomerase A3, collagen-binding protein precursor, mitochondrial stress-70 protein and...

Ngày tải lên: 20/02/2014, 01:20

... N-acetylornithine deacetylase; OCT, ornithine carbamoyltransferase; ASS, argininosuccinate synthase and ASL, argininosuccinate lyase by arginine [16,17] As a result, the concentration of citrulline and ... mitochondria [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32] In plants, information on the localization of citrulline and arginine biosynthetic ... owing to its high thermostability and insensitivity to the inhibition induced by citrulline and arginine However, many different enzymes are involved in the metabolism of citrulline (Fig 1), and...

Ngày tải lên: 20/02/2014, 03:20

... oxidatively during aging Proc Natl Acad Sci USA 95, 12896–12901 19 Martinez, M., Hernandez, A.I., Martinez, N & Ferrandiz, M.L (1996) Age-related increase in oxidized proteins in mouse synaptic mitochondria ... with aging (Eur J Biochem 270) 2299 Western blotting of modified proteins Fig Determination of CML-protein content in liver mitochondrial matrix with aging The CML content in the matrix proteins ... washed three times with NaCl/Pi containing 0.05% (v/v) Tween 20 (buffer A) and free binding sites were blocked by incubation for h at room temperature with 100 lL NaCl/ Pi containing 6% (w/v) skimmed...

Ngày tải lên: 20/02/2014, 11:20

... According to Buchbinder and Reed [21], Na+ and K+ bind to the divalent cation binding site I Thus, the inhibition shown in Fig would result from the Selectivity of pyruvate kinase for Na+ and K+ ... Solvation and energetic of binding of Na+ and K+ to pyruvate kinase It has been hypothesized [18] that the difference between Na+ and K+ in the activation and inhibition of pyruvate kinase could ... active and inactive conformations of pyruvate kinase [17,29,43] The spectral center of mass of pyruvate kinase and pyruvate kinase in complex with Mg2+ and phosphoenolpyruvate in the presence of Na+ ,...

Ngày tải lên: 08/03/2014, 02:20

Ngày tải lên: 15/03/2014, 23:22

... hypochlorite from bleach, 60 mm NaCO3, 0.4 m NaOH) for 30 min, and this was followed by a rinse in 1% sodum thiosulfate (5 min) and multiple washings in ice-cold 0.25 m NaCl as previously described ... kit (Invitrogen), following the manufacturer’s protocol Briefly, total RNA was treated with calf intestinal alkaline phosphatase to remove 5¢-phosphates, a step that eliminates truncated mRNA and ... voltage-dependent anion channel, hexokinase, creatine kinase, the mitochondrial peripheral benzodiazepine receptor, adenine nucleotide translocase (ANT), cyclophilin D (CypD) and the phosphate carrier have...

Ngày tải lên: 29/03/2014, 00:20

... data reported in the present study, and obtained in living cells, confirm the finding obtained in reconstituted proteoliposomes [11,12] and isolated membranes [29,30] indicating that Pgp and a bacterial ... anthracyclines, are positively charged and therefore bind to acidic groups in proteins and membranes Thus, their movement into the cell can be envisaged as a series of binding and releasing events ... obtained by sequential exposure of cells to increasing concentrations of doxorubicin and was maintained in the presence of 0.5 lm doxorubicin 2008 parental cells and their MRP1 over-expressing...

Ngày tải lên: 29/03/2014, 08:20

... proteins containing a series of amino-terminus-truncated human L-PGDS The amino-terminal amino acid residue of each mutant is indicated on the left The signal sequence of the amino-terminal 22 amino ... mass and anionic property However, the origin of urinary L-PGDS remains unclear Furthermore, only 10% of L-PGDS administered intravenously in canines is recovered in the urine [21], suggesting ... proteins in the lipocalin gene family L-PGDS is dominantly expressed in the brain, heart and male genital organs [1], is secreted into various body fluids, such as CSF, plasma, seminal plasma and...

Ngày tải lên: 30/03/2014, 01:20

... (2003) Dynamic interaction between the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts: evidence from lightinduced cross-linking of the proteins in the ... assembly and mechanism of binding Plant Cell 9, 441–452 Miyao, M & Murata, N (1989) The mode of binding of three extrinsic proteins of 33 kDa, 23 kDa and 18 kDa in the Photosystem II complex of spinach ... DpsbJ and DpsbL, and particularly DpsbE and DpsbF, showed clear deficiencies in their PSII assemblies The PSII monomer was missing from DpsbE and DpsbF and was present only in minor amounts in DpsbL...

Ngày tải lên: 30/03/2014, 13:20

... abilities to impair NER by inhibiting induction of p53 and XPC and to activate MMR by maintaining high levels of MSH2 and enhancing platinum accumulation in tumors NaAsO2 and hyperthermia might not ... were the kind gift of Dr Eddie Reed Cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum, 100 μg/mL penicillin/streptomycin, mM L-glutamine and 0.2 units/mL insulin Cells ... to cisplatin-DNA damage involves the induction of DNA repair proteins to initiate DNA repair [10] We determined if NaAsO2 and hyperthermia modulated the expression of XPC, a platinum-DNA damage...

Ngày tải lên: 20/06/2014, 07:20

... in tree growth in Finland: results from the 6th National Forest Inventory, Folia For 407 (1979) 1–12 [67] Tomppo E., National forest inventory in Finland and its role in estimating the carbon ... Scots pine (Pinus sylvestris L.) in Finland using stand, site and climate factors, For Ecol Manage 205 (2005) 215–225 Carbon sink of Finland’s forests [62] Ståhl G., Boström B., Lindkvist H., Lindroth ... Resource Information, United Nations, New York and Geneve, 1992 [76] Vanninen P., Mäkelä A., Fine root biomass of Scots pine stands differing in age and soil fertility in southern Finland, Tree...

Ngày tải lên: 07/08/2014, 16:20