naïve b cells in bm

... dimethylmethylene blue (DMMB) assay The beads were solubilised by incubation for 20 minutes at 4°C in dissolving buffer containing 55 mM sodium citrate, 30 mM Na2EDTA and 0.15 M NaCl, pH 6.8 The resulting ... would be of benefit for regenerating the degenerate disc Previous studies investigating the effect of CDMP1 on rabbit disc cells in monolayer [9] and mouse [22] and bovine disc cells in alginate ... 37°C) Following washing, non-specific binding sites were blocked at room temperature for 45 minutes in 25% w/v donkey serum in 1% w/v BSA (Sigma, Gillingham, Dorset, UK) Sections were incubated overnight...

Ngày tải lên: 09/08/2014, 14:22

... heterodimer, but the larger b subunit, in addition to possessing both the polymerase and RNase H domains found in the a subunit, also contains a C-terminal region corresponding to the viral integrase ... JW & Le Grice SF (2004) ‘Binding, bending and bonding’: polypurine tract-primed initiation of plus-strand DNA synthesis in human immunodeficiency virus Int J Biochem Cell Biol 36, 1752–1766 Schultz ... activity EMBO J 9, 1171– 1176 Wohrl BM & Moelling K (1990) Interaction of HIV-1 ribonuclease H with polypurine tract containing RNA– DNA hybrids Biochemistry 29, 10141–10147 DeStefano JJ, Mallaber LM,...

Ngày tải lên: 30/03/2014, 02:20

... dependent increase in CT for RNA amplification was also noted, with incubation temperatures of 25°C and 30°C resulting in smaller increases in CT values for RNA detection than incubation at 35°C Incubation ... theoretically eliminated as a source of template that would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact ... prepared in MARC-145 cells maintained in Dulbecco's Modified Eagle medium supplemented with 10% heat-inactivated fetal bovine serum and mM Lglutamine, 0.25 µg/ml fungizone, and 0.5 mg/ml gentamycin...

Ngày tải lên: 19/06/2014, 08:20

... dependent increase in CT for RNA amplification was also noted, with incubation temperatures of 25°C and 30°C resulting in smaller increases in CT values for RNA detection than incubation at 35°C Incubation ... theoretically eliminated as a source of template that would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact ... prepared in MARC-145 cells maintained in Dulbecco's Modified Eagle medium supplemented with 10% heat-inactivated fetal bovine serum and mM Lglutamine, 0.25 µg/ml fungizone, and 0.5 mg/ml gentamycin...

Ngày tải lên: 20/06/2014, 04:20

... )pb 621( 5214 0004 )pb 57( 5951 1251 )pb 821( 279 548 )nocilpma fo ezis( noitisoP ARMAT htiw delebal saw dne '3 eht saerehw AMF-6 htiw delebal saw seborp fo dne '5 ehT atad knaBneG elbaliava ... no seiduts dehsilbup ylsuoiverp fo rebmun A noissucsiD IPD dna IPD neewteb RCP-TR lanoitnevnoc yb detceted eb dluoc ANR lariv dna suriv evil htob ,revewoH IPD.3 dna IPD neewteb ANR lariv rof evitisop ... 1002/53/GKU 141935JA 99/AHC/tebiT 831935JA VOB/99/2/WAT 731935JA srebmun niarts suriV noissecca knabeneG epytoreS sniarts VDMF fo noitpircseD la te elba T meO uK eaJ 802 .RCP-TR T/R yb tcaer ton did detset...

Ngày tải lên: 07/08/2014, 18:21

... cited in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp Page of (page number ... type PCR product was inserted into the TA cloning vector before digesting with BbsI, an enzyme with no recognition sites inside the vector and only one site in the insert The linearized plasmid ... F, Hiscott J and Wainberg MA Diminution of CD4 surface protein but not CD4 messenger RNA levels in monocytic cells infected by HIV-1 AIDS 1991, 5(1):2933 Gaumer HR Optimizing the comparison of...

Ngày tải lên: 11/08/2014, 08:20

... was defined by TCID50, while susceptibility to the single cycle SIVsm543-GFP construct was defined as the % GFP+ PBMC 48 hrs post-infection Rhesus monkey PBMC exhibited varied susceptibility ... SIVsm543-GFP Following infection of rhesus monkey B- LCL with this construct, we observed a broad range in B- LCL susceptibility to SIV infection, varying from 1% to 30% of target cells infected (Fig ... SIVsmE543-GFP infection was defined as % GFP+ PBMC following infection with VSV-G pseudotyped SIVsmE660-GFP determined using flow cytometric analysis (B) Positive correlation between rhesus monkey PBMC...

Ngày tải lên: 12/08/2014, 04:20

... Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China and 2Qingdao Agricultural University, Qingdao 266109, China ... are maintained in the Harbin Veterinary Research Institute A total of 30 field samples (lung, spleen, liver, or bladder) were collected from different farms in Heilongjiang and Jilin provinces ... vaccine strains Amplification was not possible for nonCDV viruses, such as CPV, CAV, CCV, RV, NDV-infected cells, and uninfected cells by RT-nPCR (Fig 1) Sensitivity, applicability, and repeatability...

Ngày tải lên: 12/08/2014, 04:20

... RRM domains are depicted by boxes and the numbers refer to amino acid positions in the full length proteins The putative binding site for HIV-1 RT is indicated by the arrow at the C-terminus of ... of mRNA stabilizing proteins that interact with AU-rich elements (ARE), sharing Page of significant sequence similarity with the Drosophila RNAbinding protein ELAV (embryonic lethal abnormal vision) ... protein constructs and investigated their RT interaction by biophysical methods We did not find any evidence for a direct interaction between HIV-1 RT and HuR by NMR chemical shift mapping in the...

Ngày tải lên: 12/08/2014, 23:23

... for beta-globin was generated using DNA extracted from BLV negative sheep blood cells Detection and quantification of the clonal distribution of circulating BLV positive cells in vivo BLV integration ... U5 PBS PPT U3 R 5’ 3’ R U5 PBS PPT U3 R PBS PPT U3 R R U5 R U5 R U5 U3 R U5 U3 R U5 PBS PPT PBS PPT + PBS U3 R U5 PBS PBS U3 PBS R U5 NlaIII NlaIII U3 R U5 PBS U3 R U5 U3 R U5 PBS U3 R U5 10 B ... estimated by trypan blue dye exclusion [39] PBMCs were labeled with monoclonal antibodies (Mabs) directed against surface immunoglobulin M (anti-sIgMs, clone 1H4, mouse IgG1; Pig45A2, mouse IgG 2b) ,...

Ngày tải lên: 13/08/2014, 06:20

... viral infection in dividing and nondividing C8166 T cells Effect of IN mutants on viral infection in dividing and nondividing C8166 T cells To test the effect of different IN mutants on HIV-1 infection ... next investigated the intracellular localization of truncated IN- YFP fusion proteins in HeLa cells by using indirect immunofluorescence assay, as described above Results showed that the IN5 0–288-YFP ... N-terminus of YFP protein The cDNA encoding for each IN- YFP fusion protein was inserted in a SVCMV expression plasmid B) Expression of different IN- YFP fusion proteins in 293T cells 293T cells were transfected...

Ngày tải lên: 13/08/2014, 09:21

... glycerol backbone remains Depending on the number of hydrocarbon tails attached to the glycerol group, between one and three FAMEs can be obtained These FAMEs may have hydrocarbon chains of the ... any biobased content A B5 algal fuel was prepared by splash blending All runs were conducted at full loading and 2500 rpm with a 13.4-kW, inline, three-cylinder, direct-injection diesel engine ... showed no variation, within experimental uncertainty, while CO was negligible for both fuels Elevated unburned hydrocarbons indicate inefficiencies in the combustion process The B5 yielded significantly...

Ngày tải lên: 05/09/2013, 15:28

... hemagglutination inhibition tests in the diagnosis of influenza A and B virus infections”, Purified hemagglutinin in subtype-specific diagnosis, J Vir.Methods 10, 75-84 34 Kandun IN, Wibisono H, ... T, Shinya K, Sawada T, Kiso M, Usui T, Murata T, Lin Y, Hay A, Haire LF, Stevens DJ, Russell RJ, Gamblin SJ, Skehel JJ, Kawaoka Y (2006), “Haemagglutinin mutations responsible for the binding ... Emerg Infect Dis, 10, pp 1321- Baigent SJ, McCauley JW (2001), “Glycosylation of haemagglutinin and stalk-length of neuraminidase combine to regulate the growth of avian influenza viruses in tissue...

Ngày tải lên: 10/02/2014, 20:39

... were interested in using reverse-joined hairpin ribozymes as building blocks for the construction of twin ribozymes In order to evaluate the structural properties of reverse-joined hairpin ribozymes ... labeling of RNA using a small engineered ribozyme Chembiochem in press 19 Feldstein A, Buzayan JM & Bruening G (1989) Two sequences participating in the autolytic processing of satellite tobacco ... [34,35] Because of the single-stranded linker, FEBS Journal 272 (2005) 4464–4474 ª 2005 FEBS An engineered ribozyme for RNA sequence exchange joining the two domains in reverse-joined hairpin ribozymes,...

Ngày tải lên: 20/02/2014, 01:20

... synset as input However, in Question Answering (QA) scenarios we not have such information To obtain it we employ a strategy which uses WordNet along with pattern-based web mining Our web mining part ... can be obtained for the desired object Also, since the above studies utilize only explicitly available information they were unable to approximate object values in cases where no explicit information ... patterns in order to extract attribute values of these objects and available comparison/boundary information Finally, we analyze the obtained information and select or approximate the attribute value...

Ngày tải lên: 20/02/2014, 04:20

... by restricting the number of edges connected to a node Wikipedia’s Infoboxes In order to provide query specific background knowledge we make use of Wikipedia’s infoboxes These infoboxes contain ... due to some intentionally introduced breaks (e.g., denoted by betweens text fragments) On the other hand, we want to benefit from PoS tagging during chunk recognition in order to be able to identify, ... distance between For the remainder of the paper N=1000 We are using Bing (http://www.bing.com/) for Web search 22 Concerning the English PoS tags, “word/PoS” expressions that match the following regular...

Ngày tải lên: 20/02/2014, 05:20

... following components: (i) a finite-state machine toolkit for building, combining, and optimizing finite-state devices; (ii) a flexible XML-based regular compiler for converting regular patterns into ... applying the unifiability test Such information can in fact be precompiled This and other optimization techniques are described in (Krieger and Piskorski, 2003) When compared to symbol-based finite ... edges in our automata are annotated by TFSs, instead of atomic symbols However, not every outgoing edge in such an automaton must be analyzed, since TFS annotations can be arranged under subsumption,...

Ngày tải lên: 20/02/2014, 16:20

... Japanese text Because of the disadvantage inherent in reading difficulty involved in all symbols being strung together without any logical break between words, the morpheme dictionary, including more ... a set of syllables, when there are S different kinds of syllables ( S = 101 in Japanese) The syllable candidates at the saxne position in input phonetic text are located in one S bit space Therefore, ... I1: I li (e) Figure 4: Movement in Shift Register Block t I (d) N-th Character Memory store the sub-string of input text, which can be shifted, as shown in Fig The index memory receives a character...

Ngày tải lên: 21/02/2014, 20:20

... found it in bone mineral density (BMD), the amount of calcium and other minerals contained in bone They reasoned: • More than 99 percent of the body’s calcium is found in teeth and bones • Bone is ... studies discussed in Chapters through are listed in the appendixes References to studies cited in the rest of the book can be viewed by visiting: Build ingBoneVitality.com Abstracts of all studies ... authorities have been scratching their heads about it for more than twenty years They have suggested several possible explanations Vitamin D Deficiency Vitamin D boosts the body’s ability to absorb calcium...

Ngày tải lên: 05/03/2014, 20:20

... would be turned into fat and stored No big deal Because how often did they find a beehive? Soon they’d be back to eating game, their insulin levels would drop, and they’d shift right back to burning ... insulin resistance and the insulin levels in your blood drop like a rock Your body stops socking away everything you eat into fat storage and starts acting like the dual-fuel machine it is, burning ... The kind in a big oval chunk Brown a slice or two in butter to eat with eggs or cut cubes to scramble into them Brown a slice of ham per customer in butter, flip, and while the other side is browning,...

Ngày tải lên: 06/03/2014, 05:20