naïve b cells in bm
Báo cáo y học: "Expression of cartilage-derived morphogenetic protein in human intervertebral discs and its effect on matrix synthesis in degenerate human nucleus pulposus cells" potx
... dimethylmethylene blue (DMMB) assay The beads were solubilised by incubation for 20 minutes at 4°C in dissolving buffer containing 55 mM sodium citrate, 30 mM Na2EDTA and 0.15 M NaCl, pH 6.8 The resulting ... would be of benefit for regenerating the degenerate disc Previous studies investigating the effect of CDMP1 on rabbit disc cells in monolayer [9] and mouse [22] and bovine disc cells in alginate ... 37°C) Following washing, non-specific binding sites were blocked at room temperature for 45 minutes in 25% w/v donkey serum in 1% w/v BSA (Sigma, Gillingham, Dorset, UK) Sections were incubated overnight...
- 10
- 437
- 0
Báo cáo khoa học: Ribonuclease H: properties, substrate specificity and roles in retroviral reverse transcription ppt
... heterodimer, but the larger b subunit, in addition to possessing both the polymerase and RNase H domains found in the a subunit, also contains a C-terminal region corresponding to the viral integrase ... JW & Le Grice SF (2004) ‘Binding, bending and bonding’: polypurine tract-primed initiation of plus-strand DNA synthesis in human immunodeficiency virus Int J Biochem Cell Biol 36, 1752–1766 Schultz ... activity EMBO J 9, 1171– 1176 Wohrl BM & Moelling K (1990) Interaction of HIV-1 ribonuclease H with polypurine tract containing RNA– DNA hybrids Biochemistry 29, 10141–10147 DeStefano JJ, Mallaber LM,...
- 11
- 296
- 0
Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
... dependent increase in CT for RNA amplification was also noted, with incubation temperatures of 25°C and 30°C resulting in smaller increases in CT values for RNA detection than incubation at 35°C Incubation ... theoretically eliminated as a source of template that would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact ... prepared in MARC-145 cells maintained in Dulbecco's Modified Eagle medium supplemented with 10% heat-inactivated fetal bovine serum and mM Lglutamine, 0.25 µg/ml fungizone, and 0.5 mg/ml gentamycin...
- 8
- 678
- 0
báo cáo hóa học:" Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" ppt
... dependent increase in CT for RNA amplification was also noted, with incubation temperatures of 25°C and 30°C resulting in smaller increases in CT values for RNA detection than incubation at 35°C Incubation ... theoretically eliminated as a source of template that would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact ... prepared in MARC-145 cells maintained in Dulbecco's Modified Eagle medium supplemented with 10% heat-inactivated fetal bovine serum and mM Lglutamine, 0.25 µg/ml fungizone, and 0.5 mg/ml gentamycin...
- 8
- 475
- 0
Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
... )pb 621( 5214 0004 )pb 57( 5951 1251 )pb 821( 279 548 )nocilpma fo ezis( noitisoP ARMAT htiw delebal saw dne '3 eht saerehw AMF-6 htiw delebal saw seborp fo dne '5 ehT atad knaBneG elbaliava ... no seiduts dehsilbup ylsuoiverp fo rebmun A noissucsiD IPD dna IPD neewteb RCP-TR lanoitnevnoc yb detceted eb dluoc ANR lariv dna suriv evil htob ,revewoH IPD.3 dna IPD neewteb ANR lariv rof evitisop ... 1002/53/GKU 141935JA 99/AHC/tebiT 831935JA VOB/99/2/WAT 731935JA srebmun niarts suriV noissecca knabeneG epytoreS sniarts VDMF fo noitpircseD la te elba T meO uK eaJ 802 .RCP-TR T/R yb tcaer ton did detset...
- 6
- 347
- 0
Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... cited in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp Page of (page number ... type PCR product was inserted into the TA cloning vector before digesting with BbsI, an enzyme with no recognition sites inside the vector and only one site in the insert The linearized plasmid ... F, Hiscott J and Wainberg MA Diminution of CD4 surface protein but not CD4 messenger RNA levels in monocytic cells infected by HIV-1 AIDS 1991, 5(1):2933 Gaumer HR Optimizing the comparison of...
- 4
- 319
- 0
Báo cáo y học: " Variability in a dominant block to SIV early reverse transcription in rhesus monkey cells predicts in vivo viral replication and time to death" pot
... was defined by TCID50, while susceptibility to the single cycle SIVsm543-GFP construct was defined as the % GFP+ PBMC 48 hrs post-infection Rhesus monkey PBMC exhibited varied susceptibility ... SIVsm543-GFP Following infection of rhesus monkey B- LCL with this construct, we observed a broad range in B- LCL susceptibility to SIV infection, varying from 1% to 30% of target cells infected (Fig ... SIVsmE543-GFP infection was defined as % GFP+ PBMC following infection with VSV-G pseudotyped SIVsmE660-GFP determined using flow cytometric analysis (B) Positive correlation between rhesus monkey PBMC...
- 11
- 340
- 0
Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China and 2Qingdao Agricultural University, Qingdao 266109, China ... are maintained in the Harbin Veterinary Research Institute A total of 30 field samples (lung, spleen, liver, or bladder) were collected from different farms in Heilongjiang and Jilin provinces ... vaccine strains Amplification was not possible for nonCDV viruses, such as CPV, CAV, CCV, RV, NDV-infected cells, and uninfected cells by RT-nPCR (Fig 1) Sensitivity, applicability, and repeatability...
- 6
- 480
- 0
Báo cáo y học: " The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro" doc
... RRM domains are depicted by boxes and the numbers refer to amino acid positions in the full length proteins The putative binding site for HIV-1 RT is indicated by the arrow at the C-terminus of ... of mRNA stabilizing proteins that interact with AU-rich elements (ARE), sharing Page of significant sequence similarity with the Drosophila RNAbinding protein ELAV (embryonic lethal abnormal vision) ... protein constructs and investigated their RT interaction by biophysical methods We did not find any evidence for a direct interaction between HIV-1 RT and HuR by NMR chemical shift mapping in the...
- 7
- 258
- 0
Báo cáo y học: "Early and transient reverse transcription during primary deltaretroviral infection of sheep" ppt
... for beta-globin was generated using DNA extracted from BLV negative sheep blood cells Detection and quantification of the clonal distribution of circulating BLV positive cells in vivo BLV integration ... U5 PBS PPT U3 R 5’ 3’ R U5 PBS PPT U3 R PBS PPT U3 R R U5 R U5 R U5 U3 R U5 U3 R U5 PBS PPT PBS PPT + PBS U3 R U5 PBS PBS U3 PBS R U5 NlaIII NlaIII U3 R U5 PBS U3 R U5 U3 R U5 PBS U3 R U5 10 B ... estimated by trypan blue dye exclusion [39] PBMCs were labeled with monoclonal antibodies (Mabs) directed against surface immunoglobulin M (anti-sIgMs, clone 1H4, mouse IgG1; Pig45A2, mouse IgG 2b) ,...
- 12
- 217
- 0
Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import" pps
... viral infection in dividing and nondividing C8166 T cells Effect of IN mutants on viral infection in dividing and nondividing C8166 T cells To test the effect of different IN mutants on HIV-1 infection ... next investigated the intracellular localization of truncated IN- YFP fusion proteins in HeLa cells by using indirect immunofluorescence assay, as described above Results showed that the IN5 0–288-YFP ... N-terminus of YFP protein The cDNA encoding for each IN- YFP fusion protein was inserted in a SVCMV expression plasmid B) Expression of different IN- YFP fusion proteins in 293T cells 293T cells were transfected...
- 15
- 416
- 0
Harvesting, oil extraction, and conversion of local filamentous algae growing in wastewater into biodiesel
... glycerol backbone remains Depending on the number of hydrocarbon tails attached to the glycerol group, between one and three FAMEs can be obtained These FAMEs may have hydrocarbon chains of the ... any biobased content A B5 algal fuel was prepared by splash blending All runs were conducted at full loading and 2500 rpm with a 13.4-kW, inline, three-cylinder, direct-injection diesel engine ... showed no variation, within experimental uncertainty, while CO was negligible for both fuels Elevated unburned hydrocarbons indicate inefficiencies in the combustion process The B5 yielded significantly...
- 6
- 488
- 0
Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm a h5n1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... hemagglutination inhibition tests in the diagnosis of influenza A and B virus infections”, Purified hemagglutinin in subtype-specific diagnosis, J Vir.Methods 10, 75-84 34 Kandun IN, Wibisono H, ... T, Shinya K, Sawada T, Kiso M, Usui T, Murata T, Lin Y, Hay A, Haire LF, Stevens DJ, Russell RJ, Gamblin SJ, Skehel JJ, Kawaoka Y (2006), “Haemagglutinin mutations responsible for the binding ... Emerg Infect Dis, 10, pp 1321- Baigent SJ, McCauley JW (2001), “Glycosylation of haemagglutinin and stalk-length of neuraminidase combine to regulate the growth of avian influenza viruses in tissue...
- 11
- 581
- 0
Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt
... were interested in using reverse-joined hairpin ribozymes as building blocks for the construction of twin ribozymes In order to evaluate the structural properties of reverse-joined hairpin ribozymes ... labeling of RNA using a small engineered ribozyme Chembiochem in press 19 Feldstein A, Buzayan JM & Bruening G (1989) Two sequences participating in the autolytic processing of satellite tobacco ... [34,35] Because of the single-stranded linker, FEBS Journal 272 (2005) 4464–4474 ª 2005 FEBS An engineered ribozyme for RNA sequence exchange joining the two domains in reverse-joined hairpin ribozymes,...
- 11
- 481
- 0
Tài liệu Báo cáo khoa học: "Extraction and Approximation of Numerical Attributes from the Web" pdf
... synset as input However, in Question Answering (QA) scenarios we not have such information To obtain it we employ a strategy which uses WordNet along with pattern-based web mining Our web mining part ... can be obtained for the desired object Also, since the above studies utilize only explicitly available information they were unable to approximate object values in cases where no explicit information ... patterns in order to extract attribute values of these objects and available comparison/boundary information Finally, we analyze the obtained information and select or approximate the attribute value...
- 10
- 465
- 0
Tài liệu Báo cáo khoa học: "A Mobile Touchable Application for Online Topic Graph Extraction and Exploration of Web Content" ppt
... by restricting the number of edges connected to a node Wikipedia’s Infoboxes In order to provide query specific background knowledge we make use of Wikipedia’s infoboxes These infoboxes contain ... due to some intentionally introduced breaks (e.g., denoted by betweens text fragments) On the other hand, we want to benefit from PoS tagging during chunk recognition in order to be able to identify, ... distance between For the remainder of the paper N=1000 We are using Bing (http://www.bing.com/) for Web search 22 Concerning the English PoS tags, “word/PoS” expressions that match the following regular...
- 6
- 458
- 0
Tài liệu Báo cáo khoa học: "Integrating Information Extraction and Automatic Hyperlinking" docx
... following components: (i) a finite-state machine toolkit for building, combining, and optimizing finite-state devices; (ii) a flexible XML-based regular compiler for converting regular patterns into ... applying the unifiability test Such information can in fact be precompiled This and other optimization techniques are described in (Krieger and Piskorski, 2003) When compared to symbol-based finite ... edges in our automata are annotated by TFSs, instead of atomic symbols However, not every outgoing edge in such an automaton must be analyzed, since TFS annotations can be arranged under subsumption,...
- 4
- 356
- 0
Tài liệu Báo cáo khoa học: "A HARDWARE ALGORITHM FOR HIGH SPEED MORPHEME EXTRACTION AND ITS IMPLEMENTATION" pptx
... Japanese text Because of the disadvantage inherent in reading difficulty involved in all symbols being strung together without any logical break between words, the morpheme dictionary, including more ... a set of syllables, when there are S different kinds of syllables ( S = 101 in Japanese) The syllable candidates at the saxne position in input phonetic text are located in one S bit space Therefore, ... I1: I li (e) Figure 4: Movement in Shift Register Block t I (d) N-th Character Memory store the sub-string of input text, which can be shifted, as shown in Fig The index memory receives a character...
- 8
- 504
- 0
Building bone vitality: A Revolutionary Diet Plan to Prevent Bone Loss and Reverse Osteoporosis pdf
... found it in bone mineral density (BMD), the amount of calcium and other minerals contained in bone They reasoned: • More than 99 percent of the body’s calcium is found in teeth and bones • Bone is ... studies discussed in Chapters through are listed in the appendixes References to studies cited in the rest of the book can be viewed by visiting: Build ingBoneVitality.com Abstracts of all studies ... authorities have been scratching their heads about it for more than twenty years They have suggested several possible explanations Vitamin D Deficiency Vitamin D boosts the body’s ability to absorb calcium...
- 257
- 268
- 1
Glycemic Load Diet Cookbook: 150 RECIPES TO HELP YOU LOSE WEIGHT AND REVERSE INSULIN RESISTANCE docx
... would be turned into fat and stored No big deal Because how often did they find a beehive? Soon they’d be back to eating game, their insulin levels would drop, and they’d shift right back to burning ... insulin resistance and the insulin levels in your blood drop like a rock Your body stops socking away everything you eat into fat storage and starts acting like the dual-fuel machine it is, burning ... The kind in a big oval chunk Brown a slice or two in butter to eat with eggs or cut cubes to scramble into them Brown a slice of ham per customer in butter, flip, and while the other side is browning,...
- 286
- 421
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25