mutations and polymorphisms in colorectal cancer

... apoptosis in colorectal cancer cell lines [13,14] In this study we investigated whether doxycycline works in synergy with cisplatin and oxaliplatin or not in HT 29 colorectal cancer cell line Methods ... activity levels in cells treated with cisplatin and oxaliplatin in combination with doxycycline (p = 0.001 and p = 0.039 respectively) These findings corresponded with the findings obtained in caspase ... Discussion Colorectal cancer constitutes the third most common cancer and the second leading cause of cancer deaths in UK About one in 20 men develops colorectal cancer in their lifetime (Cancer...

Ngày tải lên: 09/08/2014, 04:20

... These surprising findings suggest that TCF4 might be the main binding partner for b-catenin during development and progression of colorectal cancer whereas an enhancement of Wnt/bcatenin transcriptional ... The DNA binding proteins and transcription factors TCF4 and LEF-1 are partners of nuclear b-catenin and effectors of the Wnt/b-catenin signalling pathway, which is decisively involved in tumorigenesis ... exclusive in colorectal cancer In accordance with published literature we found nuclear b-catenin positivity in 75% of cases In contrast LEF-1 expression was found only in 26% and TCF4 in 46% of colorectal...

Ngày tải lên: 18/06/2014, 16:20

... The human colorectal cancer cell lines LoVo, SW480, HT29, HCT116 and LS174T were maintained in RPMI 1640 (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum at 37°C in a 5% ... in colorectal cancer CK2a protein expression was analyzed in 144 patients (104 with CRC and 40 with colorectal adenoma) Staining for CK2a was nearly negative in all of the normal Page of 11 colorectal ... expression in colorectal cancers, adenomas and adjacent normal colorectal mucosa Staining was (A) negative in normal colorectal epithelium cells, (B, C) weak to moderate in the nuclei of colorectal...

Ngày tải lên: 18/06/2014, 19:20

... secretagogin, a protein expressed in neuroendocrine cells of the colonic crypts in carcinomatous mucosal cells involved in calcium-binding The study concluded that expression of secretagogin in non-neuronal ... resistance in CRC highlighted in this review included Hsp27 (described previously), Anexin IV (like secretagogin, another calcium-binding protein) as well as 14-3-3sigma (a protein involved in regulation ... chemoradiotherapy in rectal cancer [24] In their study, 46 patients (31 for training and 15 for validation testing) with rectal carcinoma underwent preoperative RCT and surgical excision weeks later Baseline...

Ngày tải lên: 09/08/2014, 04:21

... of deviating skipping -3 -2 -1 Relative amounts of deviating inclusion skewed TIN (sTIN): Preferential exon skipping or inclusion overall TIN (oTIN): High or low total amounts of deviating exon ... scanned image files containing pixel intensity values CEL data files were used as input for preprocessing and alternative splicing detection with the Finding Isoforms using Robust Multichip Analysis ... deviating exon usage as tumors with transcriptome instability (TIN) Tumors showing preferential exon skipping or inclusion - that is, having a skewed distribution in the amounts of deviating skipping...

Ngày tải lên: 11/08/2014, 12:21

... 10% of all cancer incidence and mortality in 20132 Consistent with trends in developed countries, CRC is the most frequent cancer in Singapore, accounting for 14% to 18% of all cancer incidence ... 2005-20093 Colorectal cancers usually begin as non-cancerous polyps in the inner lining of the colon or rectum4 Over many years, adenomatous polyps in the dysplasic colon may develop into pre-neoplastic ... to initiate metastatic processes in vitro, through promoting metastatic protein expression, as well as modulating oncogenic and mesenchymal transcription STMN1 silencing on the other hand reinstates...

Ngày tải lên: 09/09/2015, 11:25

... activity, and showed that CDO alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating ... reduction in cyclin D and E expression Disappointingly, the specific interactions between CDKs and cyclins were not assessed, and the effects of CDO on p21waf1 were not investigated, making direct ... those seen in cells cultured with SO and drug (all P < 0.001), indicating a synergistic effect of CDO and cytotoxic drug in inducing senescence (Fig 5) Cyclin-associated CDK protein levels are...

Ngày tải lên: 07/03/2014, 15:20

... trained in, and maintain a special interest in, techniques relevant to colorectal cancer, and who can demonstrate a high level of skill in this area Each surgeon in the MDT should carry out a minimum ... three main tasks The first was to breathe new life into dialogue about services for managing bowel cancer by highlighting key issues and enhancing and clarifying the original text In refocusing attention ... cancer, in terms of both incidence and mortality, in England and Wales Although prostate cancer is more common in men and breast cancer more common in women, colorectal cancer affects both sexes.2...

Ngày tải lên: 28/03/2014, 23:20

... effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells in G2/M At the same time, reduced levels of cyclins ... determined the expression of the uncharacterized gene, KIAA0247, in human gastrointestinal tissues and colonic cell lines Results indicated that KIAA0247 ubiquitously expresses in gastrointestinal ... Progress and challenges in colorectal cancer screening and surveillance Gastroenterology 2010, 138:2115-2126 Kim MS, Lee J, Sidransky D: DNA methylation markers in colorectal cancer Cancer Metastasis...

Ngày tải lên: 18/06/2014, 19:20

... effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells in G2/M At the same time, reduced levels of cyclins ... determined the expression of the uncharacterized gene, KIAA0247, in human gastrointestinal tissues and colonic cell lines Results indicated that KIAA0247 ubiquitously expresses in gastrointestinal ... Progress and challenges in colorectal cancer screening and surveillance Gastroenterology 2010, 138:2115-2126 Kim MS, Lee J, Sidransky D: DNA methylation markers in colorectal cancer Cancer Metastasis...

Ngày tải lên: 20/06/2014, 03:20

... been fully investigated in ovarian cancer In the present study, we examined the relationship between the expression of Ets-1 and its targets Ang-2 and maspin in ovarian cancer and their clinical ... suppressor by inhibiting tumor cell motility, invasion, metastasis and angiogenesis [12] Maspin expression is aberrantly silenced in many human cancers including breast, prostate, and thyroid cancer ... roles in angiogenesis: Ang-1 and Ang-4 are agonist ligands for Tie2 and induce tyrosin phosphorylation of Tie2, while Ang-2 and Ang-3 are antagonist ligands They bind to Tie2 without inducing tyrosin...

Ngày tải lên: 10/08/2014, 10:21

... procured locally Cell line and culture conditions The human lung cancer cell line A549 was obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in RPMI-1640 supplemented ... anticancer effect of Osthole has been reported in a few papers Both in vitro and in vivo studies showed that Osthole possessed an anticancer effect by inhibiting human cancer cells growth and inducing ... Cdc2/Cyclin B1, Bcl-2 protein and up-regulated Bax in our study In summary, these results indicated that Osthole induced G2/M arrest and apoptosis possibly by down-regulating Akt signaling in human...

Ngày tải lên: 10/08/2014, 10:21

... response in regional lymph nodes can inform prognosis in colorectal cancer 10 11 12 Acknowledgements and funding We thank Mandy Fisher and Spencer Walker for technical assistance and Adam Girardin ... difficulties of using immune tissue from patients with an immune mediated inflammatory disease However, an interesting finding was the difference between colorectal cancer patients and inflammatory ... or CD8+Foxp3+ cells in cancer patients and control IBD patients This last finding was interesting considering previous work Association between T cell populations and other clinico-pathological...

Ngày tải lên: 10/08/2014, 10:21

... transmembrane glycoproteins: the extracellular domain has the ligand binding site and also contains specific sequences that are involved in dimerization, while the intracellular domain is a catalytic ... predictive value of KRAS mutations and polymorphisms of EGFR and IgG-Fc-receptor in 40 patients with metastatic CRC receiving cetuximab-containing chemotherapy (in combination with irinotecan, FOLFIRI, ... [15] determined the KRAS mutation status and mRNA expression levels of the EGFR ligands amphiregulin and epiregulin in 95 patients with primary CRC treated with cetuximab and irinotecan and correlated...

Ngày tải lên: 10/08/2014, 22:20

... cervical and ovarian cancer EZN-2208 undergoing evaluation in phase trials for treatment of metastatic breast and colorectal cancer (www.clinicaltrials.gov) Doxorubicin and Anthracyclines Inhibits binding ... that can influence cell survival and growth through changes in several signaling pathways that are involved in protein synthesis, autophagy, apoptosis and metabolism [29] Intriguingly, mTOR and HIF1 ... E1B 19 kDa protein-interacting protein 3; CAD, carboxy-terminal transactivation domain; CAIX, Carbonic anhydrase 9; CDKN1A, Cyclin-dependent kinase inhibitor 1A; FIH, factor inhibiting HIF; GLUT1,...

Ngày tải lên: 11/08/2014, 12:21

... of Interventions for colorectal Cancer in EURC sub-region Note: Interventions falling above the broken line are not cost-effective, Interventions falling between the broken and continuous line ... of Interventions for colorectal Cancer in AFRE sub-region Note: Interventions falling above the broken line are not cost-effective, Interventions falling between the broken and continuous line ... 10% of colorectal cancer causing polyps are within reach of the examining finger There is therefore no solid basis for recommending this method as a stand alone screening intervention for colorectal...

Ngày tải lên: 13/08/2014, 11:22

... and the role of this pathway in rapamycin sensitivity in cancer therapy; To examine the combination therapy by using PDK1 inhibitor and mTOR inhibitor in colorectal cancer The present study identifies ... strategy in treating rapamycin-resistant colorectal cancer 32 Chapter II: Materials and Methods 33 2.1 Cell lines and cell culture 2.1.1 Colorectal cancer cell lines The human colorectal cancer ... signaling and oncogenic transformation, making PIK3CA a druggable target in cancer therapy (Liu et al., 2009) Many PI3K inhibitors have been developed in clinical setting, including pan-PI3K inhibitors,...

Ngày tải lên: 09/09/2015, 18:52

... 2005), colorectal- cancer mortality has doubled in both men and women over the past three decades in Singapore The rising trend in incidence and mortality highlights the importance of understanding ... factors in colorectal cancer, molecular markers may also be useful prognostic and predictive indicators in colorectal cancer as they have been shown to be in other cancers In breast cancer, for ... caspases/direct IAP-binding protein with low pI 10 Both the intrinsic and extrinsic are the two main interlinked pathways that can induce apoptosis by activation of caspases (Figure 1.2) The extrinsic pathway...

Ngày tải lên: 14/09/2015, 08:50

... designed and synthesized by Charles Heidelberger in 1957 (Heidelberger et al 1957) It has been widely used for treating a range of cancers, including colorectal and breast cancers, and cancers ... developed to increase the anti -cancer activity of 5-FU and prevent clinical resistance The combination of 5-FU with other drugs including leucovorin, methotrexate (MTX), oxaliplatin and irinotecan ... buffer and stained with antibodies against surface antigens (CD3 and CD4 or CD8) for 30min in the dark at 4°C Cells were subsequently washed in staining buffer twice prior to fixation For FOXP3 and...

Ngày tải lên: 03/10/2015, 20:57