ms instrumentation triple quadrupole and ion trap

Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc

Báo cáo khoa học: Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry doc

... concentration of 52 lm The reaction mixture was incubated at 30 °C for min, and a portion was then analyzed by LC -MS To the rest of the reaction mixture propionyl-CoA was added to a final concentration ... in combination with on-line liquid chromatography ion trap mass spectrometry studies on the multifunctional proteins, DEBS1-TE, DEBS3 and DKS as model systems, introduces an additional powerful ... addition, the observation of acyl-intermediates on ATL-ACPL and KS1 demonstrates that the formed acyl-enzyme intermediates are stable under the digestion and analytical conditions used here, and...

Ngày tải lên: 16/03/2014, 18:20

15 210 0
Practical instrumentation for automation and process control 2

Practical instrumentation for automation and process control 2

... selecting and implementing industrial instrumentation systems and control valves It can be argued that a clear understanding and application of the instrumentation and control valves systems is the ... industrial use Understand the problems with installing measurement equipment Troubleshoot instrumentation systems and control valves Isolate and rectify instrumentation faults Understand most of the ... Introduction 1.5 Instrumentation for Automation and Process Control P&ID (Process and Instrumentation Diagram) Symbols Graphical symbols and identifying letters for Process measurement and control...

Ngày tải lên: 01/01/2014, 21:15

374 452 3
Báo cáo khoa học: Implication for buried polar contacts and ion pairs in hyperthermostable enzymes pot

Báo cáo khoa học: Implication for buried polar contacts and ion pairs in hyperthermostable enzymes pot

... homodimers Their Z score and rmsd values range ˚ ˚ between 14.8 and 7.0 A and between 1.07 and 2.38 A, FEBS Journal 274 (2007) 4012–4022 ª 2007 The Authors Journal compilation ª 2007 FEBS 4015 4016 ... score and Strucural elememts providing hyperthermostability ˚ rmsd values range between 15.3 and 12.4 A and ˚ , respectively The sequence between 1.47 and 1.66 A similarity varied between 35% and ... aminomaltase from T aquaticus, the Z score and rmsd values of the other ˚ AMs range between 13.3 and 10.0 A and between ˚ , respectively, representing fairly good 1.41 and 2.52 A structural similarity...

Ngày tải lên: 07/03/2014, 05:20

11 534 0
Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx

Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx

... 50 to 200; and MSMS mode with m ⁄ z 147.1 as the precursor ion and collision energy at 20.5% Fractions containing the hydrazide reaction product were also collected, lyophilized and analyzed ... products, both in vivo [12,42] and in vitro [43] 7064 Evidence for coordination of condensation and ketoreduction In previous work [44] the operation of a single extension module from DEBS was studied ... evidence for the proposed quaternary effects, suppression of condensation and tandemization, is more circumstantial but deserves further study MS also has the potential to play a major supporting...

Ngày tải lên: 16/03/2014, 03:20

13 426 0
Silicon as a model ion trap: Time domain measurements of donor Rydberg states

Silicon as a model ion trap: Time domain measurements of donor Rydberg states

... these equations represents the feeding of the ground state by decay from the excited state at rate 1/T1 and recombination of the electrons and ions with rate Pg This recombination is proportional ... of the electron and ion densities, and therefore scales like ni2 The other equations are similarly interpreted, and Ptot ϭ Pg ϩ Px Px describes relaxation between the continuum and the excited ... SCIENCES Multiphoton Ionization If we take the absorption cross-section for 1s(A1) 2p0 to be ␴abs ϳ ϫ 10Ϫ18 m2 from the small-signal absorption spectrum, and note that the Si transmission coefficient...

Ngày tải lên: 16/03/2014, 15:30

5 296 0
Báo cáo khoa học: Roles of adenine anchoring and ion pairing at the coenzyme B12-binding site in diol dehydratase catalysis pptx

Báo cáo khoa học: Roles of adenine anchoring and ion pairing at the coenzyme B12-binding site in diol dehydratase catalysis pptx

... 13480195 and 17370038 and Priority Areas 753 to T Toraya, and (C) 14580627 to T Tobimatsu] from the Japan Society for Promotion of Science and the Ministry of Education, Culture, Sports, Science and ... enzyme upon the Ala substitution, and slightly lowered upon the Gln substitution at the b135 residue This might be due to hydrogen bonding and interactions other than the ion pairing being strong ... sequencing of the DNA region encompassing the entire diol dehydratase genes and tac promoter that no unintended mutations had been incorporated during mutagenesis Expression and purification of mutant diol...

Ngày tải lên: 23/03/2014, 06:20

13 380 0
Báo cáo khoa học: Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol–gel matrix doc

Báo cáo khoa học: Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol–gel matrix doc

... intermediates in the P450 reaction Results and Discussion Formation and decay of oxyferrous PGIS complex in solution Upon reduction of the ferric PGIS heme by dithionite, the Soret peak was blue-shifted ... Fig Absorption spectra of PGIS and its ligand complexes in solution and in sol–gel Binding with (A) U46619, (B) NaCN and (C) clotrimazole Spectra were recorded before (solid lines) and after (dashed ... solution containing re-oxidized PGIS and sealed the cuvette with parafilm Again, the Soret peak was gradually red-shifted and after h of incubation it reached 422 nm, whereas the a bands and b bands...

Ngày tải lên: 23/03/2014, 07:20

10 422 0
Báo cáo khoa học: Azotobacter vinelandii rhodanese Selenium loading and ion interaction studies potx

Báo cáo khoa học: Azotobacter vinelandii rhodanese Selenium loading and ion interaction studies potx

... maintained at 23 °C, and the protein concentration was kept constant at lM The excitation and emission bandwidths were and nm, respectively The excitation wavelength was set at 286 nm, and the spectra ... whether there is transition between the forms of the enzyme after addition of these ions Figure 3C,D shows the spectrum of the ES form after addition of phosphate and hypophosphite ions No changes in ... Azotobacter vinelandii highlights the evolutionary relationship between the rhodanese and phosphatase enzyme families J Mol Biol 298, 691–704 Ó FEBS 2003 Rhodanese interaction with ions and selenium...

Ngày tải lên: 31/03/2014, 07:20

8 327 0
basic instrumentation measuring devices and basic pid control

basic instrumentation measuring devices and basic pid control

... Science and Reactor Fundamentals – Instrumentation & Control CNSC Technical Training Group i Table of Contents Section - OBJECTIVES Section - INSTRUMENTATION EQUIPMENT 2.0 2.1 INTRODUCTION ... Neutron Detection 78 REVIEW QUESTIONS - EQUIPMENT 81 Revision – January 2003 Science and Reactor Fundamentals – Instrumentation & Control CNSC Technical Training Group ii Section - CONTROL ... Note Science and Reactor Fundamentals – Instrumentation & Control CNSC Technical Training Group INSTRUMENTATION EQUIPMENT 2.0 INTRODUCTION Instrumentation is the art of measuring the value of...

Ngày tải lên: 28/05/2014, 14:12

125 415 1
Báo cáo hóa học: " Lateral homogeneity of the electronic properties in pristine and ion-irradiated graphene probed by scanning capacitance spectroscopy" pot

Báo cáo hóa học: " Lateral homogeneity of the electronic properties in pristine and ion-irradiated graphene probed by scanning capacitance spectroscopy" pot

... data and wrote the article SS carried out the sample preparation, the measurements and participated to the analysis of the data ER worked on the evaluation of ion- graphene interaction cross sections ... morphological and spectroscopic characterization of graphene on SiO2 Phys Status Solidi C 2010, 7:1251 14 Compagnini G, Giannazzo F, Sonde S, Raineri V, Rimini E: Ion irradiation and defect formation in ... modulating bias between the substrate and the tip locally induces a shift of the graphene quasi-Fermi energy EF in the conduction band, and, hence, an accumulation of electrons at the SCM Electronic...

Ngày tải lên: 21/06/2014, 05:20

8 292 0
Báo cáo hóa học: " Lateral homogeneity of the electronic properties in pristine and ion-irradiated graphene probed by scanning capacitance spectroscopy" potx

Báo cáo hóa học: " Lateral homogeneity of the electronic properties in pristine and ion-irradiated graphene probed by scanning capacitance spectroscopy" potx

... data and wrote the article SS carried out the sample preparation, the measurements and participated to the analysis of the data ER worked on the evaluation of ion- graphene interaction cross sections ... morphological and spectroscopic characterization of graphene on SiO2 Phys Status Solidi C 2010, 7:1251 14 Compagnini G, Giannazzo F, Sonde S, Raineri V, Rimini E: Ion irradiation and defect formation in ... modulating bias between the substrate and the tip locally induces a shift of the graphene quasi-Fermi energy EF in the conduction band, and, hence, an accumulation of electrons at the SCM Electronic...

Ngày tải lên: 21/06/2014, 06:20

8 400 0
Báo cáo toán học: "Steiner triple systems and existentially closed graphs" ppsx

Báo cáo toán học: "Steiner triple systems and existentially closed graphs" ppsx

... intersection graph of every Steiner triple system, with the exception of the unique systems on and points, is 2-e.c Furthermore, we obtain a characterization of those Steiner triple systems whose ... Steiner triple systems and configurations Steiner triple systems A Steiner triple system of order v, STS(v), is a pair (V, B) where V is a set of cardinality v of elements, or points, and B is ... The 4-block Pasch configuration and the 5-block mitre configuration play key roles in the theory of configurations in Steiner triple systems The construction of systems which avoid either or both...

Ngày tải lên: 07/08/2014, 13:21

12 339 0
Báo cáo toán hoc:" Spherical f-Tilings by Scalene Triangles and Isosceles Trapezoids III " ppsx

Báo cáo toán hoc:" Spherical f-Tilings by Scalene Triangles and Isosceles Trapezoids III " ppsx

... f-tilings of S whose prototiles are Q and T From now on Q is a spherical isosceles trapezoid of internal angles α1 and α2 (α1 > α2 ) and edge lengths a, b and c (b > c), and T is a spherical scalene ... triangle and sharing the side of length c, and also that we cannot have two trapezoids sharing the sides of length a and c, respectively These situations have already been studied before ([7, 8]) and ... are two trapezoids sharing the side of length c Consequently, a trapezoid must always have an adjacent trapezoid sharing the side of length c; and, on the other hand, we cannot have two trapezoids...

Ngày tải lên: 08/08/2014, 01:20

39 259 0
Ion trap cavity quantum electrodynamics

Ion trap cavity quantum electrodynamics

... cavity enhances the interaction between the ion and a single photon, and enables efficient collection of the ion emissions Proposed applications of trapped ion- cavity systems in QIP include quantum ... with trapped ions Moreover, recent demonstrations of entanglements between trapped ions and photons [15], and between distant trapped ions [16, 17] lay the ground work for quantum networks and ... a + q /2 /2 and ωz = Ω a /2 φx,y,z are the phases determined by the initial conditions of the ion position and velocity The solutions of Equation 2.7 and Equation 2.8 comprise motions in two...

Ngày tải lên: 10/09/2015, 09:05

180 561 0
Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"

Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"

... enough specificity and sensitivity [30-34] Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is one useful tool for integrating separation and analysis of ... construct a classification tree (Figure 5) Figure shows the tree structure and sample distribution The classification tree using the combination of the four peaks identified 98 CRC and 80 healthy controls ... Identification of Biomarker Pattern and Construction of Diagnostic Model The decision tree could differentiate samples of CRC and healthy controls Here, we used the biomarker wizard function of the...

Ngày tải lên: 25/10/2012, 11:18

9 530 1
Instrumentation Symbols and Identification

Instrumentation Symbols and Identification

... system diagrams, loop diagrams, logic diagrams • Functional descriptions • Flow diagrams: Process, Mechanical, Engineering, Systems, Piping (Process) and Instrumentation • Construction drawings ... Application to industries 2.3 Application to work activities 2.4 Application to classes of instrumentation and to instrument functions 10 2.5 Extent of functional identification ... instrumentation and to instrument functions The symbolism and identification methods provided in this standard are applicable to all classes of process measurement and control instrumentation They...

Ngày tải lên: 04/04/2013, 12:40

72 547 0
Ecological Assessment of Water Quality by Three-species Acute Toxicity Test and GC/MS Analysis - A Case Study of Agricultural Drains

Ecological Assessment of Water Quality by Three-species Acute Toxicity Test and GC/MS Analysis - A Case Study of Agricultural Drains

... similar to the preparation of samples for ecotoxicity test, and finally acetone solution was analyzed by GC /MS RESULTS AND DISCUSSION Ecotoxicity Level of River Water Histograms of each ecotoxicity ... 10mL Acetone  Pure water Filtration Solid phase extraction Nitrogen purge Concentration factor adjustment Test solution Fig - Illustration of the solid-phase extraction procedure Three-species Ecotoxicity ... for the inhabitation of aquatic organisms The results of the measurement and the calculation of DVi, DIi and DI are shown in Table Three chemicals, terbucarb (MBPMC), bromobutide and tolclofos-methyl,...

Ngày tải lên: 05/09/2013, 10:15

8 670 0
Configuring SNMP Security and Traps

Configuring SNMP Security and Traps

... Public will appear in the box by default Step Under Trap destinations click Add and type the Host name, the name of your PC, in the box Click Apply, and then OK Close all windows Troubleshooting Before ... If access is gained to the device, intruders can obtain device information and possibly change the configurations Reflection What is the default SNMP community name? ... any host Click Apply, and then OK What is the purpose of a community name? Step From the SNMP Service Properties window, click on the Traps tab Under Community...

Ngày tải lên: 19/10/2013, 03:15

2 281 0
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