mouse the bird and the sausage

... to the sausage to bring in the wood, to the mouse to cook, and to the bird to fetch the water And now what happened? The sausage started in search of wood, the bird made the fire, and the mouse ... arranging the work Beg and pray as the mouse and the sausage might, it was of no use; the bird remained master of the situation, and the venture had to be made They therefore drew lots, and it fell ... the best of things and to remain with one another So now the bird set the table, and the mouse looked after the food and, wishing to prepare it in the same way as the sausage, by rolling in and...

Ngày tải lên: 20/10/2013, 07:15

... hobbyists in Asia and Europe, and animals from these fancy mouse colonies were used to generate the common strains of the laboratory mouse [69,70] It is also these house mouse species, the mice in ... characterization of these viruses should further elucidate their evolutionary past and describe their pathogenic potential and the adaptations that favor co-existence of these infectious agents and their new ... and the yellow block marks the range of the species carrying M/Pmvs The blue line is the Yangtze River which roughly coincides with the transition between M castaneus and musculus [66], and the...

Ngày tải lên: 13/08/2014, 01:20

... table and the test statistics of these were used to form an estimate of their distribution under the null hypothesis, to which the observed test statistic can be compared Additional data files The ... used in the analysis Additional data file contains the sequences of the proteins used in the analysis of the assignments The sequences assignments file in the used in Phyletic assignments the proteins ... eukaryote-specific proteins and universal proteins - present in prokaryote species Then we compared the expression pattern of the different categories within various mouse tissues and studied the tendency of...

Ngày tải lên: 14/08/2014, 14:21

... at the bottom EDTA extracts of mouse heart (d) and kidney (j) were used at the dilutions shown at the top used this tumor and also mouse kidney and muscle for successive extraction with EDTA and ... slope to that of the reference inhibitors b1IV and b2IV, respectively Together the data showed that these assays were highly speci®c and suitable for the quantitation of b1 and b2 chain epitopes ... eluted in front of the b1IV dimer from a molecular sieve and showed a broad electrophoretic band mainly in the range 70±110 kDa, which could be converted into the monomer and dimer bands after treatment...

Ngày tải lên: 21/02/2014, 03:20

... has the same angle as the base of the ramp Knowing the angle of the base of the ramp and the weight of the car on the ramp, we can solve for any other force including the force acting down the ... angles The difference between the two forces causes the force down the ramp to increase The greater the angle required to move the car, the more friction there will be acting against the car’s ... be called the rolling friction The smaller the coefficient of friction, the more efficient your mousetrap car and the greater the travel distance will be Rolling Friction Finding the theoretical...

Ngày tải lên: 16/03/2014, 12:20

... is also present in the TH lanes The other is likely too close to the more prominent TH band to be visible likely to represent the long and short isoforms of the enzyme Many of the tissues included ... over the other For instance in the spleen, the short isoform is the predominant AK species, whereas in the testis and kidney, the long isoform is more abundant Most brain regions, with the exception ... at these and other sites using a standard kit (Stratagene) and following the manufacturer’s recommendations for mutagenic primer design Mutations were confirmed by DNA sequencing along both strands,...

Ngày tải lên: 16/03/2014, 18:20

... (–) (–) mBSE Chandler (+) (+) (–) (+) (–) 20 15 Fig The effects of EDTA and digitonin on PMCA reactions (A) The effect of the indicated concentrations of EDTA and imidazole on the PMCA reactions ... ultrasensitive detection, and also minimize the risk of contamination The approximately 10-fold difference in the sensitivity between mBSE and Chandler may be caused by differences between the minimum size ... clear PrPSc bands (Fig 3A, lanes and 2) and a further sample exhibited less distinct bands (Fig 4A, lane 3) After the secondround reactions, three samples produced strong PrPSc bands (Fig 4B,...

Ngày tải lên: 23/03/2014, 04:21

... the figures The times of exposure were the same for Fig 2A and B The reproducibility of the Western blot quantification was analyzed using mouse gastrocnemius and tibialis muscle mitochondria The ... validation of the antibodies used The Lilly antibody to mouse and rat UCP3 [6] and the Chemicon antibody to human UCP3 (AB3046) [7] were found to react specifically with mouse UCP3, as the signal ... Fig 3C, the UCP3/COX ratio in SS mitochondria is higher in the gastrocnemius than in the soleus (1.5-fold) and in IMF mitochondria in the tibialis anterior and in the gastrocnemius than in the soleus...

Ngày tải lên: 24/03/2014, 04:21

... of bands for each immunoblot processed, but for a given blot the intensity of the egg band can be compared to the intensity of the oocyte band To summarize the three experiments for each of the ... isoforms, the band intensities for oocyte and egg lanes in a given blot were analyzed using NIH image and compared to each other The intensity of the oocyte band was normalized to 100% for each blot and ... same blot For every blot, the density of the band for oocyte extracts was normalized to 100% and the density of the band for eggs is given as a percent with regards to the normalized oocyte value...

Ngày tải lên: 21/06/2014, 19:20

... physiological functions and intracellular signaling Thus, our system provides an accessible method to examine the endothelial cell biology of the mouse, and will accelerate the molecular and cellular analysis ... Osaka, Japan) The RNA was reverse-transcribed using SuperScript II (Invitrogen) and used for cloning the SV40T Ag cDNA The cDNA encoding the wild-type SV40T Ag and the 3¢ portion of the tsA58T Ag ... lipoproteins (LDLs) The cell populations derived from the brain, lung, heart, liver and uterus stained positive for CD31 (Fig 3B for the brain, liver and uterus; data not shown for the lung and heart),...

Ngày tải lên: 18/02/2014, 17:20

... disease, the primary pathology is in the kidney [1], the major site of Gb3 synthesis in man [11], and in the heart, possibly due to the association of Gb3 synthesis with the microvasculature GSL synthesis ... and glycogen breakdown [23] Unlike all other GSLs, GlcCer is made on the outer leaflet of the Golgi bilayer [24] and must be ‘flipped’ into the lumen to access the glycosyltransferases for further ... synthesis in the Fabry mouse model Our studies have further delineated the abnormal Gb3 synthesis in this model and have shown that the inhibition of MDR1 is a viable potential approach to the reduction...

Ngày tải lên: 19/02/2014, 07:20

... provided by the Harvard Brain Tissue Resource Center (Belmont, MA, USA) and the Netherlands Brain Bank (Amsterdam, The Netherlands) Blocks of cortex, anterior amygdala, striatum and midbrain ... accordance with the literature [16–18] The difference in the expression levels in the liver of the ADH4–/– and wild-type mice (Fig 1C) might indicate that the transgenic manipulation at the ADH4 locus ... positive and all cerebellar Purkinje cells were strongly positive Expression in the granular layer of rat and mouse cerebellum was moderate In hippocampus, neurons in the hilus of the dentate gyrus and...

Ngày tải lên: 21/02/2014, 00:20

... motifs: the Walker A and B motifs, the signature or LSGGQ motif and the A-, D-, H- and Q-loops These motifs are positioned around the bound nucleotide and help to position and maintain it in the ... contacts the c-phosphate of the bound nucleotide across the dimer [15] and the aromatic residue of the A-loop Abcb1a catalytic mechanism stacks against the adenine moiety of bound nucleotide and provides ... with the putative catalytic water and a helix extending from the TMD, may be involved in signal transduction between the TMD and NBD, by sensing either hydrolysis of the terminal phosphate or the...

Ngày tải lên: 07/03/2014, 06:20

... by the lower arrow to the left of the autoradiograph) All bands were quantified using the Scion Image program and the ratio between the upper and lower bands for each assay is presented below the ... All bands were quantified using the Scion Image program The upper numbers represent the intensity of each band normalized to the band in lane The lower numbers represent the intensity of the bands ... therefore analyzed the sequence of the R2 promoter and the 5¢-UTR, and compared it to the corresponding region in the human R2 gene We could identify a potentially interesting palindrome in the...

Ngày tải lên: 08/03/2014, 02:20

... 3) Further, the two wash fractions (lanes and 5) and also the fraction eluted with 0.2 M KCl (lane 6) did not form complexes with the DNA probe The 0.5 M and M KCl eluates, on the other hand, ... boxes I-III (CSB), tRNA genes, L and H strand promoters (LSP and HSP), L and H strand transcription start sites (LSS and HSS) The L-strand sequence and position of the putative promoter distal transcription ... suggested that the distal gene transcription proceeds until the end of the D-loop, overlapping with the HSP [19,20] In the mouse system, the 3¢ end of the D-loop H-strand RNA maps to the CAA sequence...

Ngày tải lên: 08/03/2014, 08:20

... duplicate and were repeated at least three times RESULTS Northern blot analysis reveals the difference in the expression pattern between Mer and the other Tyro family members (Axl and Rse) in mouse ... interactions within the )321/)26 region of the mouse Mer promoter The )321/)181 (A) and) 181/)26 (B) fragments were labelled on the antisense strand and subjected to DNase I cleavage in the presence ... PCR reaction was performed using the original pGL3/()321/+126) as the template and the product from the first PCR reaction as the primer In step three, MP-D and either GLprimer2 (first PCR reaction...

Ngày tải lên: 08/03/2014, 23:20

... in cells of the gland ducts and mucous glands [9] In the adult lung, the expression of MUC5B follows a restricted pattern, with a positive gradient from the surface to the glands and a decrease ... TTF-1 and GATA factors the development of studies aimed at the identification of the biological role of Muc5b in the lung employing mouse models Results Characterization of the sequence of the promoter ... throughout the sequence GATA putative binding sites are present in both the proximal and distal parts of the promoter Alignment of the human and mouse promoter sequences showed that there is a...

Ngày tải lên: 15/03/2014, 00:20

... Glu, Ser and Thr residues, the absence of Lys, His and Arg except at the termini, and the fact that it is completely unstructured based on the absence of medium- and long-range NOEs and the observation ... further structural elements at their C termini, including the ‘BG loop’ and the ‘G’-strand (Fig 2) [24] Hortner et al [25] have modelled the structure of the SOCS3 SH2 domain and suggest that the ... whilst residues 22–28 are unstructured The helix encompasses the entire ESS and the four residues at the C terminus of the KIR The remaining residues that comprise the KIR appear to be unstructured...

Ngày tải lên: 16/03/2014, 14:20

... (Table 1) Mouse EST sequences for p65 are found in the oocyte, pre-implantation embryos and in the testis On the other hand, p50 ⁄ Nf-jb1 EST sequences are found in the oocyte and in the testis ... was confirmed in the testis and established in the TM3 and TM4 testicular cell lines by RT-PCR and western blot analyses (Fig 5) It is noted in the western blots that while the p50 and p65 levels ... of the p65 and p50 NF-jB subunit proteins in testicular cells (A) RT-PCR detection of p50 and p65 transcripts in the testis (Te) and in TM3 and TM4 cells (B) Western blot analysis of the p50 and...

Ngày tải lên: 29/03/2014, 00:20

... remaining in the fibroblast was plotted vs time, and the rate constant for the taurine efflux (min)1) at each time point was estimated as the negative slope of the graph between the time point and the proceeding ... M) and used for estimation of the protein content using the Lowry procedure [19] and BSA as protein standard The residual 1000 lL of the suspension was centrifuged (20 000 g, 10 min) and the ... lM) plus theophylline (0.5 mM), and H2O2 (2 mM) were included in the experimental medium from the time of the initiation of the influx experiment The influx was estimated from the slope of the uptake...

Ngày tải lên: 30/03/2014, 15:20