... dysfunction. ACKNOWLEDGEMENTS We thank Drs Carlos E. Argaran ˜ a and Mario Guido for critical reading of the manuscript, Mrs S. N. Deza and Mrs M. G. Schachner for technical assistance and Dr Stephen Anderson ... Secretarı ´ adeCienciayTe ´ cnicadelaUniver- sidad Nacional de Co ´ rdoba y Agencia Co ´ rdoba Ciencia del Gobierno de la Provincia de Co ´ rdoba, Argentina. REFERENCES 1. Barra, H.S., Arce, C .A. , ... into alpha-tubulin by recombinant mammalian tubulin-tyrosine ligase. Biochim. Biophys. Acta 1481, 131–138. 13. Fukuyama, N., Takebayashi, Y., Hida, M., Ishida, H., Ichimori, K. & Nakazawa,...
Ngày tải lên: 17/03/2014, 10:20
Ngày tải lên: 18/06/2014, 17:20
ON WEAK SOLUTIONS OF THE EQUATIONS OF MOTION OF A VISCOELASTIC MEDIUM WITH VARIABLE BOUNDARY V. G. pptx
Ngày tải lên: 23/06/2014, 00:20
Tài liệu Báo cáo khoa học: Influence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx
... mechanochemical nature of a- CT catalysis A more detailed analysis of the influence of chemical glycosylation on the dynamics of a- CT from the theor- etical simulations was additionally performed to gain a deeper ... modeled. Table S4. Average rmsd (A ˚ ) for the conserved catalytic residues calculated from MD simulations of a- CT and the various Lac -a- CT conjugates modeled. This material is available as part of ... were calculated with YASARA’s analysis module. Gaussian network model analysis GNM analysis represents a simplified version of normal mode analysis for describing the fluctuations in dynamics of polymer...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspecific alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf
... kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (c, 250 kDa) were loaded on to a separate gradient as molecular mass markers. Novel aggregate formation of an alkaline phosphatase frame-shift ... fraction). BSA (b, 68 kDa), alcohol dehydrogenase (a, 141 kDa) and catalase (250 kDa) were loaded on to a separate gradient as mole- cular mass markers. K. Komaru et al. Novel aggregate formation of an alkaline ... Lafferty MA, Slaughter C, Raducha M & Harris H (1986) Isolation and characteri- zation of a cDNA encoding a human liver ⁄ bone ⁄ kid- ney-type alkaline phosphatase. Proc Natl Acad Sci USA 83,...
Ngày tải lên: 19/02/2014, 17:20
Tài liệu Báo cáo khoa học: The single tryptophan of the PsbQ protein of photosystem II is at the end of a 4-a-helical bundle domain docx
... r a ị where r n and r u are the experimentally determined numerical values of the ratio a/ b, and r a is the theoretical numerical value of ratio a/ b for a mixture of aromatic amino acids (Tyr and ... not mark any as poor or inappropriate. Another structural analysis, obtained by the VERIFY 3 D program [61], gave an average value of 0.21, which is greater than zero, the quality value indicated ... removing NaCl by prolonged dialysis, this protease is activated and cleaves PsbQ at low salt concentrations. We circumvented the drawbacks of the 1- M NaCl wash by taking advantage of the Cu 2+ effect....
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... mutagenesis reactions together with oligonucleo- tides ECF-Q69G d(5Â-AACAACGCAGCT GGGCTCTG GAACCAT), ECF -A1 41Q d(5Â-TCAACCTCTAAC CAG GCTACTCCGCTG) ECM-G77Q d(5Â-AACAACGCTGG C CAGCACGCTAACCAC) and ... purchased from Pharmacia Biotech (Vienna, Austria). Visualization and analysis of molecular structures Molecular structures whose coordinates were obtained from the RCSB database were visualized ... in parentheses. b Activity of SOD expressed on a per-iron metal basis (iron superoxide dismutase mutants) and per-manganese metal basis (manganese superoxide dismutase mutants). Table 2. Metal...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... 5Â GGA CAC CAC AGA TAT CCA ATA G 3Â C1011U 5Â CAT AAA ACT AAC AAT ACA TGT 3Â C1011L 5Â TAG CAG ATA TTG AAA AGG AG 3Â C12U 5Â CCA GTG AAT ATT CAG GAC TGA T 3Â C12L 5Â AGG CTC TAG CAT GAT TTG ATG ... GCA TTT TTA TTT TGA ATG TAA A 3Â C13L 5Â TAG TTC AAA TGC ACA TCT TAT 3Â C14U 5Â GGC AGA GAA TAC GTT TAT ACT AA 3Â C14L 5Â TCT CAG ATT GAC TAC TAC AAC TT 3Â C15U 5Â CAT GAA AAG CAT TTC AAA AA ... 3Â C1L 5Â CAA CTG CGT ATT AGT GAA GAA TG 3Â C2U 5Â AAT CAA ATT TTA TTA TAA GAC C 3Â C2L 5Â AAT AGC TAA TTA GAG ACC AT 3Â C3U 5Â GGT GTC AAA TTT AGC TGT TAA A 3Â C3L 5Â GCC CTC TTC TCT AAA AAG GTT...
Ngày tải lên: 22/02/2014, 04:20
Is Lactobacillus GG Helpful in Children With Crohn’s Disease? Results of a Preliminary, Open-Label Study docx
... described as medians with ranges in parentheses. The significance of changes was evaluated us- ing analysis of variance (ANOVA) for parametric variables and the Mann–Whitney test for nonparametric variables. RESULTS Four ... None had a fistula. Median duration of Crohn’s disease was 3 years (range, 1–5). All patients were taking prednisone at entry, with a median dose of 22.5 mg (range, 15–50 mg). All patients had also ... features and disease activity were assessed at baseline and at 1, 4, 12, and 24 weeks of LGG therapy. The PCDAI was calculated at each visit. Stools were cultured at each follow-up visit to assess...
Ngày tải lên: 05/03/2014, 12:20
Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
... here and is present in the S. agalactiae PPase, kinase and adenylosuccinate syn- thase, but not in S. agalactiae CovR ⁄ CsrR. The motif is located at the surface of the SaPPase (Rantanen, unpublished), ... with a pK a of 7.5 [11] to achieve catalysis, a common feature in hydro- lytic metalloenzymes [12]. One residue that appears to take part in catalysis in HsSTP is His62, which may act as a general ... fused to an EF-hand motif, and human STP (HsSTP), which has an additional 8 kDa a- helical domain at the C-terminus [3,4]. The sizes of the catalytic domains of both PPP and PPM families are well...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors potx
... [e.g. cerato-platanin of Ceratocystis fimbriata f. sp. platani, Snodprot1 of Phaeosphaeria nodorum and Sp1 of Leptosphaeria maculans) or human allergens and path- ogenesis-related proteins (As-CG of ... Stability of clades was evaluated by 1000 bootstrap rearrangements. Bootstrap values lower than 20% are not displayed in the cladogram. RNA isolation and hybridization Fungal mycelia were harvested ... helices, separated by a 14 amino acid strand. interproscan analysis [22] of Epl1 showed the affi- liation of this protein to the cerato-platanin family (IPR010829). This is a group of low molecular weight, 4-cysteine-containing...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starfish embryogenesis ppt
... ACATGTACATGTATATCACTTTGAACTGGTTTTCATTAAAAAAAAAAAACCATCAATTTG 2459 AGAAGAAACAATTACTTCTTAAGTCAATTAATTTTTCTAGAAATGCAAAAGATATTCCCC 2519 TTAACAGCTGTTTGAAATGAGGCCTCGGTCTCAAGTTTAAGAGTGCCCCCATATGTAAGC ... TAAAAAGCTCCAGGAAGTTGACCCAGAAGAAATTTGTTAAGAGTTCACGGATAAGCAAGG 2639 TATTTGGATAAGGTGCATTTGTACATTTTGTGTGTACTGGTTTAGTGTAGAATTTAATTT 2699 TTTTTGGTTAATTCTGTCACAAGAACATAATTCTATGGTTACTACACAATGTTGCATCCC ... CCGCAGCTCAGTGGTGTCAAGGCCCATGTCACACTCAATGTCAAGAGTGCTTAATTTGCT 2279 P Q L S G V K A H V T L N V K S A * ATGCGAGGTCAGCATTTATCCAACCAGAAGCTTCACGGAGCTAGCTGGGCAAGGAAATTT 2339 GATAATCGCAAGAAATAATTTCCCCCCAAAAACAAAAGGTTGTTGGCTGAAAATACTTCT 2399 ACATGTACATGTATATCACTTTGAACTGGTTTTCATTAAAAAAAAAAAACCATCAATTTG...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo Y học: Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line pptx
... from a malignant murine mast cell tumor and rat mesencephalic tegmentum. Arch. Biochem. Biophys. 199, 355–361. 13. Hasegawa, H., Yanagisawa, M., Inoue, F., Yanaihara, N. & Ichiyama, A. (1987) ... University of Science and Technology, Uenohara, Yamanashi 409–0193, Japan. Fax: + 81 554 63 4431, E-mail: hasegawa@ntu.ac.jp Abbreviations: TPH, tryptophan hydroxylase; CaM kinase II, calcium/calmodulin-dependent ... Kojima, M., Oguro, K., Sawabe, K., Iida, Y., Ikeda, R., Yamashita, A. , Nakanishi, N. & Hasegawa, H. (2000) Rapid turnover of tryptophan hydroxylase is driven by proteasomes in RBL2H3 cells, a...
Ngày tải lên: 08/03/2014, 16:20
What is the price of a mousetrap? The assessment of value from cloud services pptx
... this ebook, I discuss one functional transformation that has taken placed as a result of cloud services, namely; the impact on the assessment of value. This is a timely discourse as the vast ... being available anywhere, at anytime and on any device. If it was only a simple as a mouse-trap. The complication is that for most customers they already have an IT investment. So the assessment ... the traditional performance measure is not relevant because it doesn’t factor in agility/flexibility. Attempts to incorporate this into the traditional performance measure are absurd. It is pure...
Ngày tải lên: 09/03/2014, 02:20
Commons-based Peer-Production of Physical Goods Is there Room for a Hybrid Innovation Ecology? pdf
... couldn't find any list of where all the fab labs in the world were or what a fab lab was or is it an organisation, is it just a name, is it just a casual name, you know, like a I don't ... literature, there are a number of examples of Fab Lab projects. Mikhak et al. (2002) report on projects in India, at Vigyan Ashram Fab Lab just outside the village of Pabal in Maharashtra, and at ... logistics, lack of a central Fab Lab organisation, lacking outreach to society at large, too strong a focus on technology 11 100.00% Table 7: The pain of Fab Lab managers and assistants...
Ngày tải lên: 23/03/2014, 10:20
Báo cáo khoa học: N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell on the virion surface docx
... 3). (B) Analysis of a stan dard mixture containing Glc, Gal, Man, Fuc, GlcNAc, GalNAc, ManNAc. (C) Analysis of peak 1 (Fig. 3A) . (D) A nalysis of peak 2 (Fig. 3A) . 3140 L. A. Baratova et al.(Eur. ... virion surface Lyudmila A. Baratova 1 , Nataliya V. Fedorova 1 , Eugenie N. Dobrov 1 , Elena V. Lukashina 1 , Andrey N. Kharlanov 2 , Vitaly V. Nasonov 3 , Marina V. Serebryakova 4 , Stanislav V. ... Combined MALDI mass spectrum of material from peaks 3*, 1 and 2 after partial CPY hydrolysis. (C) M ALDI mass spectra of p eak 2* mater ial before (upper part) and after (lower part) CPY treatment...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... PKN, and rhophilin in the rho-binding domain. J. Biol. Chem 271, 13556–13560. 15. Ishizaki, T., Maekawa, M., Fujisawa, K., Okawa, K., Iwamatsu, A. ,Fujita ,A. ,Watanabe,N.,Saito,Y.,Kakizuka ,A. ,Morii,N.& Narumiya, ... PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization. MolCellBiol.17, 2247–2256. 13. Watanabe, G., Saito, Y., Madaule, P., Ishizaki, ... Furuyashiki, T., Ishizaki, T., Watanabe, G., Watanabe, N.,Fujisawa,K.,Morii,N.,Madaule,P.&Narumiya,S.(1996) Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase,...
Ngày tải lên: 23/03/2014, 21:20
TAL - A National Database of Questions - Classification is the Key pptx
Ngày tải lên: 30/03/2014, 13:20