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OReilly hadoop the definitive guide 4th edition 2015 3
... happy to share access in return for shorter analysis times, and statistically, that their analysis jobs would be likely to be spread over time, so they wouldn’t interfere with each other too ... by O’Reilly Media, Inc., 1005 Gravenstein Highway North, Sebastopol, CA 95472 O’Reilly books may be purchased for educational, business, or sales promotional use Online editions are also available ... Counters User-Defined Java Counters User-Defined Streaming Counters Sorting Preparation Partial Sort Total Sort Secondary Sort Joins Map-Side Joins Reduce-Side Joins Side Data Distribution Using...
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Tài liệu Tips for turning everyday activities into learning activities pptx
... another way to show your love share social and personal skills Help your child learn social and personal skills: A child must have social and personal skills to get along with other people, to have ... use a computer 10 11 interact social studies Help your child learn social studies: A child needs social studies skills to learn about her community and to get along with different types of people ... Maryland Family Network is to ensure that young children and their families have the resources to learn and succeed Our Programs promote high quality child care, school readiness, and family support...
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DNA, RNA and proteins
... Carries the genetic code from the nucleus to the ribosome in the cytoplasm DNA and Protein Synthesis - Translation Translation: 1) rRNA (ribosomal RNA) attaches to mRNA and starts reading the codons ... letter (E): THE DOE GBI TTH ECA T Cells have “proof reading” proteins that constantly check the DNA and fix errors, so most are corrected Protein Synthesis- you try it! DNA transcription and translation- ... Synthesis DNA and Protein Synthesis • Codon: three bases code for a specific protein: ex: AAA = Lysine • The codons code for 20 amino acids • Just like the alphabet has 26 letters to make all...
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Turning Many Projects into Few Priorities with TOC pot
... importantly, on repealed (Patrick) Buffers are used to absorb that organizational performance would be minimized variation without distraction to the resource Applying the management philosophy ... the effectively utilized to manage and maximize the priority for attention by resources should be adjusted throughput of the system Indeed, in order to so, it to address the tasks associated with ... possible candidate for this synchronizer might be a resource that is commonly used across projects and more heavily used relative to most other resources (Jacob; Newbold) project to another, and natural...
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Báo cáo khoa học: Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins docx
... positively (Arg, His, Lys) and negatively (Asp, Glu) charged residues, blue and red, respectively; hydrophilic residues (Asn, Gln, Ser, Thr), grey The figure was prepared using MOLMOL [31] probably ... buried completely, whereas the others are closer to the protein surface and are partially exposed to solvent Whereas DOPA–PpiB remained soluble, DOPA– hCypA was found in the insoluble fraction ... Remarkably, DOPA was also incorporated into HMP without apparent effects on its solubility The ortho positions of of the 12 tyrosine rings in the apo form of HMP are solvent-exposed and can readily...
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báo cáo khoa học: "Detection of DNA mismatch repair proteins in fresh human blood lymphocytes - towards a novel method for hereditary non-polyposis colorectal cancer (Lynch syndrome) screening" doc
... de-identified and processed within 24 hours to isolate lymphocytes Lymphocytes were separated by density gradient centrifugation using Lymphoprep Briefly, blood samples were diluted 2-fold with PBS, ... Page of Table Commercially available monoclonal and polyclonal antibodies used for detection of MLH1 and MSH2 proteins on western blots No Names Catalog Number Company Monoclonal Antibodies Anti-MSH2(Ab-2) ... screened a number of commercially available monoclonal and polyclonal antibodies (Table 1) using western blotting to detect full-length MLH1 and MSH2 proteins in cell lysates from established colorectal...
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Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc
... closely related to a novel family of fungal proteins than the Nnf1 family The human kinetochore protein CENP-H is more closely related to a novel family of fungal proteins than the Nnf1 family ... complex associate to form an extended stalk that is linked to two globular heads [32,33] Whether the stalk is simply a spacer or some sort of mechanical element remains unexplored Only detailed ... Albertson DG, Thomson JN: The kinetochores of Caenorhabditis elegans Chromosoma 1982, 86:409-428 Wiens GR, Sorger PK: Centromeric chromatin and epigenetic effects in kinetochore assembly Cell...
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Turning Data into Information
... managersOnly = new DataView(employees); managersOnly.RowFilter = "IsManager = true"; Visual Basic Dim managersOnly As New DataView(employees) managersOnly.RowFilter = "IsManager = True" To sort ... DataView.Sort property, using the same sort-expression syntax from the DataTable.Select method C# managersOnly.Sort = "HireDate DESC"; Visual Basic managersOnly.Sort = "HireDate DESC" You can also ... DataView managersOnly = new DataView(employees); managersOnly.RowFilter = "IsManager = true"; MessageBox.Show("Total Managers: " + managersOnly.Count); Visual Basic Dim managersOnly As New DataView(employees)...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... of genes with stronger promoters was strongly inhibited, whereas some genes possessing weaker promoters were induced It was proposed that the strong promoters were associated with accessible chromatin ... contains GG, GGG and AG motifs in one strand and GG and GTG motifs in the other, thus offering not only the highest total number of reactive motifs among the p53DBSs tested, but also the highest number ... is known to be constitutively active for sequence-specific DNA binding [16] Models of p53 latency considering the (post-translationally unmodified) p53 C-terminus solely as a negative regulator...
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Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx
... Uptake of apically and basolaterally administered glutamine was significantly different at every time point, for each concentration used Basolateral exposure of the monolayers to glutamine-containing ... high for basolaterally administered glutamine compared to apically administered glutamine Discussion Essential in this study is that the gut epithelial lining utilizes glutamine from two sources, ... mitochondrial [precursor] 60 kDa heat shock protein, mitochondrial [precursor] Protein disulfide-isomerase A3 [precursor] Protein disulfide-isomerase [precursor] Keratin, type II cytoskeletal Glutamate dehydrogenase...
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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot
... similar to those of disordered poly(Lglutamic acid) at high pH and poly(L-lysine) at low pH [18,19] Accordingly, the ROA spectra of disordered poly(Llysine) and poly(L-glutamic acid) are reproduced ... and ROA spectra of disordered poly(L-glutamic acid) (top pair) and poly(L-lysine) (middle pair) in aqueous solution at pH 3.0 and 12.6, respectively, and of native human lysozyme at pH 5.4 (bottom ... well-de®ned native fold, namely human lysozyme, in order to emphasize that different structural types of proteins usually give quite distinct ROA spectra The ROA spectrum of human lysozyme contains many...
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Báo cáo khoa học: Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria doc
... evolutionarily conserved In vitro import of Cox10p has previously been used to analyze complex IV assembly in yeast, but digitonin-solubilized mitochondria were employed and hence only supercomplexes ... blot analysis (Fig 2B, right panels) Radiolabeled Cox6c was incorporated into a complex of approximately 200 kDa that resolved at a slightly lower position than the holo-complex IV, and also into ... Cox6a was imported into isolated mitochondria for various times before solubilization in DDM and BN-PAGE analysis (Fig 3A) At early time points, Cox6a was predominantly incorporated into the...
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Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx
... sequence, respectively, competed slightly differently (Fig 2A, lanes 8–11) from Mut1 with both complexes These results show that the putative polyadenylation signal AATAAA, and also the sequences ... autoradiography South-western blotting The South-western protocol was modified from Mangalam et al [30] The proteins resolved on 12% SDS/PAGE were electroblotted onto nitrocellulose membrane using 40 mM glycine, ... the polyadenylation signal sequence (A at 16 282) the remaining two residues lie in the flanking sequences Additionally, two A residues (nucleotides 16 294 and 16 295, respectively), immediately...
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Báo cáo khoa học: Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals pdf
... negatively charged conserved residues may also act as functional monopartite NLS, with the negatively charged aspartate ⁄ glutamate (D ⁄ E) also contributing to NLS function [8] Interestingly, the ... and 63 kDa for the native homotrimers (termed long isoform, LD-DUT, and the N-terminally truncated short isoform, NTT-DUT, respectively) [27] Only LD-DUT contains the complete putative NLS sequence ... its molecular cargo either directly or indirectly through adaptor proteins such as importin a Importin b is unable to bind directly to classical nuclear targeting motifs such as the NLS of SV40...
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Báo cáo khoa học: 3¢- to 5¢ DNA unwinding by TIP49b proteins docx
... elucidated, particularly in view of such diverse functions Although ATP hydrolysis is most likely essential for the functions of these proteins [7,8], most biochemical analyses of TIP49 proteins ... Stimulation of ATP hydrolysis by TIP49b was found to be strongly dependent on oligonucleotide length, with a plateau of approximately 75% and 45% for ssM13 and ss115, respectively, indicating a correlation ... a recent review [1] Paradoxically, in principle, the presence in TIP49b of presumptive essential motifs, such as Walker A and B boxes, R-finger, and Sensor and motifs, should allow for rational...
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The future of internal audit is now Increasing relevance by turning risk into results pot
... of: • Advisory and assurance reviews • Thematic audits • Issue-based audits 6% 5% Annually 40% Semiannually Quarterly No longer an annual process, the audit plan must be refreshed regularly (e.g., ... activities but has also established itself as a strategic advisor to the business Leading trend 50%+ advisory 25%–50% advisory The key is to find the right balance between assurance and advisory when developing ... assurance and advisory Q: What percentage of the current audit plan is comprised of advisory/consulting reviews? In our survey, 90% of respondents say that advisory comprises some portion of their...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... the absence of ssDNA, hRad52 protein appeared to be so highly aggregated that it hardly entered into the gel (lane 5, Fig 2A) and was mostly in the pellet fraction following a centrifugation ... as a function of Mg2+ In the same assay, Mg2+ titration rendered hRad52 highly soluble, whereas hRad51 was highly insoluble (Fig 3B) A significant fraction of pelletable hRad52 was recovered in ... indicating that the protein was subject to solublization not only by ssDNA (Fig 2A and B), but also by Mg2+ (Fig 3B), a common effect facilitated by oppositely charged ionic species On the other hand,...
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