monoglycerides and emulsions based on crystalline monoglycerides

... 12 on beverage emulsions (Tan), by Chapter on food emulsifiers (Krog and Sparsø); by Chapter on proteins and polar lipids (Nylander); by Chapter on food emulsions in general (Dalgleish), and ... Proteins and Their Applications, edited by Srinivasan Damodaran and Alain Paraf 81 Food Emulsions: Third Edition, Revised and Expanded, edited by Stig E Friberg and K5re Larsson 82 Nonthermal ... Constantina Tzia and George Liadakis 129 Physical Principles of Food Preservation: Second Edition, Revised and Expanded, Marcus Karel and Daryl B Lund 130 Handbook of Vegetable Preservation and...

Ngày tải lên: 23/05/2014, 11:36

... dilution, anti-flag polyclonal (Sigma) at 1:5000 dilution, anti-GST polyclonal (Santa Cruz Biochemicals) at 1:10 000 dilution, anti-NS3 monoclonal (Devaron) at 1:5000 or anti-LMP7 monoclonal (Affiniti) ... pelleted and re-suspended in ml buffer A (phosphate buffered saline [PBS] containing 1mM DTT, and 1% Triton X-100) supplemented with M NaCl and mM PMSF before sonication in a Microson ultrasonic ... region of interaction between NS3 and LMP7 3.2.3 Expression and purification of recombinant NS3 and LMP7 for in vitro assays 3.2.4 Effect of LMP7 on NS3 protease activity 3.2.5 Effect of NS3 on...

Ngày tải lên: 16/09/2015, 17:12

... 849 Consensus Allowing one substitution Consensus Consensus Allowing one substitution Allowing one substitution Allowing two substitutions Allowing one substitution Allowing two substitutions ... incorporated on the basis of this Detection of nuclear fragmentation, and caspase-1, caspase-3 and caspase-8 activation Nuclear fragmentation, and caspase-1, caspase-3 and caspase-8 activation, were ... carried out using the above-mentioned sequence and its mutants (mutations at the fourth, fifth and sixth positions) are shown in Fig 1A A mobility shift of the band corresponding to [32P]ATP[cP]-labeled...

Ngày tải lên: 07/03/2014, 10:20

... membrane mimetic systems, and synthetic peptide fragments representing functional and nonfunctional fusion peptide sequences, which demonstrate that, after insertion, only functional sequences generate ... above the phase transition temperature On the one hand, SUVs were then obtained by sonication of the multilamellar vesicles at °C using a Sonics Material Vibra-CellTM sonicator Titanium debris ... centrifugation SUVs were separated from multilamellar vesicles by gel filtration on a Sepharose CL 4B column The top fractions of the SUVs peak were pooled, concentrated and stored at °C On the other hand,...

Ngày tải lên: 07/03/2014, 17:20

... binding to b1 integrin [15] Consistently, on ICAP-1-null osteoblasts and fibroblasts, fibronectin receptors are in an active conformation and b1-dependent cell adhesion is enhanced compared to that ... correlates well with, for one part, its stronger binding to Krit1 at the micromolar concentrations used in these experiments and, for the second part, with the higher contribution to the binding to ... addition to integrin activation, Rap1 induces cell polarization and facilitates cell migration Expression of activated Rap1 polarizes lymphocytes, generating a leading edge at the front and a...

Ngày tải lên: 16/03/2014, 05:20

... elution patterns of Cdc37 and Hsp90 (C) Immunoprecipitation reactions were carried out using antirat (control), anti-S pombe Cdc37 and anti-HA IgGs on fractions from the Superose 12 column containing ... pREP82-cdc37+ and are 5FOA resistant Strains with a nonfunctional cdc37 construct, cannot lose the pREP82-cdc37+ and are unable to grow on 5FOA medium Recombinant protein purification GST fusion proteins ... insoluble and therefore unable to contribute to essential Cdc37 function(s) We prepared native extracts of strains expressing various Cdc37 constructs and fractionated them by centrifugation into...

Ngày tải lên: 16/03/2014, 22:20

... Madison, WI, USA), and, for each sample, absorbance spectra were produced (Fig 5) These spectra were analysed, and, for those with strong absorption bands, the band parameters (peak position, band ... Similar to the deconvolution technique, the bandshapes of the single components are superpositions of Gaussian and Lorentzian bandshapes Best fits were obtained by assuming a Gauss fraction of 0.55–0.6 ... a-helices and lipid interactions In Protein Targeting and Translocation (Phoenix, D.A., ed.), pp 19–36 Portland Press, London 52 Zhou, Z., Macosko, J.C., Hughes, D.W., Sayer, B.G., Hawes, J & Epand,...

Ngày tải lên: 17/03/2014, 10:20

... equilibrium between two conformers Highly mobile portions of PfACP include the loop regions and helix II FabG is highly conserved across species, and is the only known isoform that functions as a ketoacyl ... PfFabG was measured by fluorescence titration [17] at 20 °C using a Jobin-Yvon Horiba spectrofluorimeter (bandpass of and nm for the excitation and emission monochromator, respectively) The fluorescence ... mm, and the corresponding decrease in the fluorescence intensity of its lone tryptophan was monitored at 334 nm A double reciprocal plot of the fluorescence intensities and ligand concentrations,...

Ngày tải lên: 23/03/2014, 10:20

... follows: bands in the regions 16961680 cm)1 and 1670 1660 cm)1, b-turn; band at 1672 cm)1, antiparallel b-sheet; band at 1651 cm)1, a-helix; band at 1640 cm)1, random coil; bands in the region 16381616 ... spectrum, and the relative areas were taken as the proportion of the related structure present Additional bands around 1610 cm)1 and 1600 cm)1, derive from side chain contributions, and were excluded ... One occurs on mammalian cell membranes, or on model membranes containing total erythrocyte lipids, and consists of tight and stable binding, followed by permeabilization via the formation of nm...

Ngày tải lên: 23/03/2014, 21:20

... localization of MFG-E8 depending on the C-domains COS-7 cells were transfected with plasmids containing MFG-E8-L (A and B), MFG-E8-S (C and D), DC1 (E and F) and DC2 (G and H) or empty plasmid (I and ... precipitated under the ultracentrifugation condition used The precipitation by ultracentrifugation at 100 000 g and solubilization by Triton X-100 strongly suggested that the secreted MFG-E8 ... cell surface distribution of MFG-E8 and contribution of each domain to the cellular localization, several domain-deletion mutant genes for MFG-E8 was constructed (Fig 1) and transiently expressed...

Ngày tải lên: 24/03/2014, 03:21

... excitation was set at 467 nm and emission at 539 nm, using nm bandpass slits in excitation and emission and a 490-nm cut off filter in the emission path Polarizers were set at 90° for the excitation and ... Y (2001) The effect of cyclization of magainin and melittin analogues on structure, function, and model membrane interactions: implication to their mode of action Biochemistry 40, 6388–6397 Matsuzaki, ... buffer and added at a concentration of 0.4 lM to the cuvette, before addition of LUVs After every addition of vesicles, the system was allowed to incubate for a few minutes Controls with addition...

Ngày tải lên: 31/03/2014, 07:20

... resulting in a biological response Antagonist is also a ligand that blocks the action of a receptor An agonist can be a full agonist, partial agonist or inverse agonist Full agonists are the compounds ... importance and were given in detail in Section 2.4 A detailed study was conducted on the mutations connected with the structure of target proteins and was explained in Section 2.4.2 Section 2.5 is ... computational chemistry and protein structure determination have concentrated on virtual screening and rapid mechanical docking methods With the advent of two-dimensional and three-dimensional structures...

Ngày tải lên: 28/11/2015, 14:01

... this interaction is dependent on tyrosine phosphorylation of tau A small proportion of expressed Y18F, Y29F, Y197F, Y310F and Y394F tau each bound to Fyn-SH2 under control conditions, and this binding ... in which exogenous tau and Fyn were expressed in non-neuronal cells, we found that only a small proportion of tau is associated with FynSH2, both under control conditions and following pervanadate ... (4G10, mouse monoclonal; Millipore, Billerica, MA, USA), Fyn (mouse monoclonal; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and GST (mouse monoclonal; GE Healthcare, Little Chalfont, UK) After...

Ngày tải lên: 14/02/2014, 14:20

... and sequence The annealing activity of the peptide was strongly inhibited at MgCl2 concentrations above mm and at NaCl concentrations above 60 mm Supporting the assumption of ionic interactions ... with the demonstrated inhibitory effect of monovalent and divalent cations on RNA binding and RNA chaperone activity [63] Interestingly, the interaction between the CTD and RNA can be monitored ... cofactor Conversely, the structure capture model assumes that, without the ligand, the RNA adopts many different transient interconverting conformations in dynamic equilibrium [22] One conformation...

Ngày tải lên: 14/02/2014, 19:20

... promiscuous protein and an essential component of the replication machinery [3,4] Replication and transcription regulation – proteins in search of their sites on the nucleic acids PCNA belongs to the ... plectoneme (interwound, as in bacteria) This distinction is relevant not only for DNA packing, but also for transcription, replication, and repair The reason for this distinction is not only ... intrinsic and protein-induced conformation effects Interactions lost in translation The 2009 Nobel prize in chemistry for studies of the structure and function of the ribosome rewarded a long-term...

Ngày tải lên: 14/02/2014, 19:20

... spans all functions— from research and development through manufacturing and production, acquisition and purchasing, and medical use—involved in the acquisition process and for all personnel, from ... and risks of the product in question FDA may also require, as a condition of approval of an NDA or BLA application, that postmarketing studies be conducted to monitor and confirm the safety and ... characterization, improved quality control and assurance, and integration of the vaccine development and production processes The contrast between vaccine production in prior years and today is...

Ngày tải lên: 17/02/2014, 11:20

... incubation at 37 °C, the reaction was quenched by the addition of 0.2 mL of a solution containing 11 m formic acid and m HCl, and heated to 90 °C for 10 The acidification of the reaction mixture ... development of an expression and purification system for hMS based on the expression host Pichia pastoris This has enabled us to investigate: (a) the potential for cobalamin incorporation mediated by other ... proposed [14] Results and Discussion Purification of hMS The ability to express hMS in a recombinant and functional form has been a major limitation in studies of the hMS and MSR redox system However,...

Ngày tải lên: 18/02/2014, 08:20

... the constructs was confirmed by restriction digestions and nucleotide sequencing pHybLex ⁄ Zeo-ER was obtained by subcloning the ER cDNA (without the first ATG codon and with the stop codon) into ... tissues and cell lines examined and the level of expression shows a minimal variation between the samples (Fig 5B) Among adult tissues, the expression of POLDIP3 was the strongest in the testis and ... stop codon) and the ER cDNA (without the first ATG codon and with the stop codon), respectively, into BamHI and NotI sites of pGEX-4T1 (Amersham, Little Chalfont, UK) in-frame with GST and pEGFP-N1...

Ngày tải lên: 19/02/2014, 05:20

... centrifugation and washed with a solution containing 500 mm KCl, 10 mm Tris ⁄ HCl, pH 7.5, 0.2 mm phenylmethylsulfonyl fluoride and stored at )80 °C Protein purification Membrane isolation and nickel-affinity ... buffer C and stored on ice, in the dark Chlorophyll extraction was performed in 80% acetone ⁄ H2O v ⁄ v, and chlorophyll concentration was calculated from absorption measurements at 663 and 646 ... mannitol medium and spotted on M9 ⁄ mannitol ⁄ agar plates containing ampicillin and different IPTG concentrations Plates were incubated at 37 °C for two days As a control, cells were plated on a Luria–Bertani...

Ngày tải lên: 19/02/2014, 06:20