monocytes macrophages in hepatocellular carcinoma

... new insights into the role of pyruvate in tumor cells during hypoxia We show here that tumor HepG2 cells are inclined to maintain extracellular pyruvate at a constant level (0.4 mm) Hypoxia inhibits ... shown) This is in line with our previous work [29] indicating enhancement of glycolysis activity in the presence of pyruvate Increased glycolysis might thus maintain the ATP supply during oxygen ... protective effects against hypoxia and reoxygenation This is mainly ascribed to its ability to maintain redox status [17], intervening in the DNA repair system [18–20] and restoring antioxidant capacities...

Ngày tải lên: 18/02/2014, 16:20

... protein (EGFP) reporter vector (pcDNA3 ⁄ EGFP-PPP6C-3¢UTR), in which the 3¢-UTR fragment of PPP6C, including the region encoding the putative binding site, was inserted downstream of the EGFP coding ... Gonadotropin-releasing hormone agonist-induced differences in granulosa cell cycle kinetics are associated with alterations in follicular fluid mullerian-inhibiting substance and androgen content J Clin ... had any effect on the intensity of EGFP fluorescence from the vector containing mutations in the region encoding the miR-373 binding sites These results show that miR-373 binds directly to the...

Ngày tải lên: 14/03/2014, 23:20

... HIP/PAP expression in hepatocellular carcinoma, we looked for proteins capable of interacting with this protein by screening a human HCC cDNA expression library in kgt11 using [32P]chimeric HIP/PAP ... (1999) Hepatocarcinoma-intestine-pancreas/pancreatic associated protein (HIP/PAP) is expressed and secreted by proliferating ductules as well as by hepatocarcinoma and cholangiocarcinoma cells ... were therefore performed using this cell line HIP/ PAP was recovered in the serum of patients with hepatocellular carcinoma which suggested that, in an in vivo setting, HIP/PAP was secreted by...

Ngày tải lên: 23/03/2014, 13:20

... CD147), matrix metalloproteinase-1 (RA MMP-1), tissue inhibitor of metalloproteinases-1 (RA TIMP-1) and blank contrast (RA blank) in the lining and sublining layers in RA synovium (× 400) The ... CD147 is high in peripheral blood monocytes in RA patients, it is highly possible that this will be maintained and will stimulate MMP production after the monocytes infiltrate into joints and differentiate ... penicillin/streptomycin and 2% L-glutamin at 37°C in a humidified atmosphere of 5% CO2 For the induction of cell differentiation, cells (5 × Invasion assay The cell invasion assay was performed using...

Ngày tải lên: 09/08/2014, 06:23

... hemireceptor; it binds to insulin receptor only at very high concentrations IGF-2 binds to IGF-1R, IGF-2R and binds to insulin receptor only during early fetal development Insulin binds to insulin receptor, ... results in no signal downstream Solid lines represent high affinity binding, dotted lines indicate weak binding and IGF-2 is the only ligand for IGF-2R [6,12,15] IGF-1 only binds to insulin receptor ... available to bind insulin receptor There are ways how insulin receptor activates the IGF axis First, when IGF-1 levels increase with IGF-1R inhibition, its binding to insulin receptor also increases,...

Ngày tải lên: 10/08/2014, 21:23

... blood monocytes rapidly increased; and circulating monocytes displayed an elevated capacity to generate CCL2/MCP-1 Acute infiltration of monocytes into the brain is thus central in early neuroinvasion ... neuroinvasion is an early and continuing process Regarding the underlying molecular mechanisms involved in BBB crossing by HIV, it appears appropriate to consider in particular the following processes: ... CCR5 in HBMECs which in turn may have enhanced the toxic interaction with the viral envelope protein [88] Interestingly, alterations in the BBB occur even in the absence of intact virus in transgenic...

Ngày tải lên: 12/08/2014, 23:23

... phosphatidylinositol 3-kinase PTEN phsophatase and tensin homolog pVHL von Hippel-Lindau protein Rb retinoblastoma RbAp retinoblastoma-associated protein RECK reversion-inducing-cysteine-rich protein ... protein Bcl B-cell lymphoma Bcr break point cluster region BH3 Bcl-2 homology domain Bid BH3 interacting domain death agonist Bmf Bcl-2 modifying factor BSA bovine serum albumin CBHA m-carboxy cinnamic ... expression in human liver cancer 57 4.1.1 HDAC1 and expression was increased in human hepatocellular carcinoma protein extracts 57 4.1.2 HDAC1 and expression was increased in human hepatocellular...

Ngày tải lên: 10/09/2015, 08:27

... Collection AXIN1 axis inhibition protein CAMs cell adhesion molecules CCRCC clear cell renal cell carcinoma CDKIs CDK inhibitors CDKN2A cyclin-dependent kinase inhibitor 2A CDKs cyclin dependent kinases ... and 5q corresponding to inactivation of TP53, RB (retinoblastoma 1), AXIN1 (axis inhibition protein 1), CDKN2A (cyclin-dependent kinase inhibitor 2A, also named p16), IGF2R (insulin-like growth ... tyrosine kinase receptor, was discovered as a retroviral oncogene, v-RAF or v-MIL, possessing a 23 serine/threonine kinase activity more than 20 years ago This serine/threonine kinase functions in...

Ngày tải lên: 11/09/2015, 16:07

... Transcription factors are modular proteins that are made up of distinct functional domains like DNA binding domains and activation domains The DNA binding domain allows for binding to specific DNA sequences ... DNA binding to take place (Landschulz, Johnson, & McKnight, 1988) The DNA binding domain of C/EBPα naturally determines the DNA binding specificity Together, the DNA binding domain and leucine ... indicating negative staining and 1-3 positive staining of increasing intensity Magnification x400 is shown 40 4.1.2 C/EBPα protein was up-regulated in human hepatocellular carcinoma tissues as compared...

Ngày tải lên: 02/10/2015, 17:14

... Unattached kinetochores will signal the activation of the spindle checkpoint (Cleveland et al., 2003) APC/C is the main inhibitory target in the spindle checkpoint as it is responsible in separating ... fixed in 4% formaldehyde in PBS for 25 minutes at 4°C followed by permeabilization in 0.2% Triton X-100 in PBS for minutes Cells were incubated in equilibration buffer for 10 minutes and labeling ... evidences have implicated PLK1 in regulating the bipolar spindle formations during onset of mitosis Centrosomal protein Ninein-like protein (Nlp) is dissociated from γ-tubulin and centrosomes upon phosphorylation...

Ngày tải lên: 16/10/2015, 15:38

... Interestingly, HMGN3 has been found to be associated with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma derived ... ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44] These factors are involved in ethanol-induced ... NM_014393 ▲ 1.52 regulation of transcription protein binding double-stranded RNA binding DNA binding ▼ 5.02 protein binding NM_001149 NM_004242 ▲- increased ▼- decreased Validation of microarray...

Ngày tải lên: 31/10/2012, 15:28

... human hepatocellular carcinoma cell line, QGY-7703, was kindly provided by D Lu (Dept of Neurology, Stanford University, Palo Alto, CA, USA) The cells were maintained in RPMI-1640 containing 10% ... growth inhibition using untreated cells as the uninhibited control Assessment of apoptosis by Annexin V staining An Annexin V–fluorescein isothiocyanate (FITC) Apoptosis Detection kit (Pharmingen, ... agents J Infect Dis 137, 122–130 27 Samuel, C., Cheng, S., Dan, L & Yong, X (2001) Taxol induced Bcl-2 protein phosphorylation in human hepatocellular carcinoma QGY-7703 cell line Cell Biol Int 25,...

Ngày tải lên: 23/03/2014, 17:22

... assay Methods Cell lines K562 cells (American Type Culture Collection) were maintained in DMEM medium Colorectal carcinoma cell lines (COLP-2 and COLM-6) were maintained in TIL Media I medium ... saline, and underwent up to nine times vaccinations via SC injection in the left inguinal area at 2-week intervals [29,30] The number of DCs used for the generation of fusions was 1–2 × 106 in ... in RPMI 1640 medium containing 1% heat-inactivated autologous serum, 1000 U/ml recombinant human GMCSF (Becton Dickinson, Bedford, MA, USA), and 500 U/ ml recombinant human IL-4 (Becton Dickinson)...

Ngày tải lên: 18/06/2014, 15:20

... reduction in cell death in CAVM1 cells An intriguing mechanism, in this regard, is the presence of scaffolding domain in caveolin1 that binds to and inhibits the activity of several signaling proteins ... trigger cell signaling remains to be determined This study analyzes whether and how CAV1 is involved in the cytotoxic and pro-apoptotic actions of RES in a human hepatocellular carcinoma (HCC) model ... using Double Polymer Staining Detection System (ZSGB-BIO, China) Immunoblotting Immunoblotting of phosphorylated ERK1/2, p38 kinase, and caspase-3 was carried out using phospho-specific MAP kinase...

Ngày tải lên: 18/06/2014, 15:20

... in HCC, including changes in the expression of p53, cyclin-dependent kinase inhibitor 1A (p21/WAF1), and murine double minute (MDM2) The tumor suppressor gene p53 plays a key role in regulating ... reported in the maxillary sinus squamous cell carcinoma [33], in Egyptian esophageal carcinoma [34], in breast carcinoma [35], prostate adenocarcinoma [36], gastric cancer [37,38], and epithelial ... hematoxylin Serum blocking solution (Reagent A) in place of the primary antibody was used as a negative control A brown particle in nuclei was considered as positive labeling Immunostaining labeling intensities...

Ngày tải lên: 18/06/2014, 15:20

... via TRIF (TIRdomain-containing adapter-inducing interferon-b) joining the RIG-I/MDA5 pathway In the other pathway, TLR7 senses single-strand HCV RNA and via the MyD88 adaptor protein activates IRAK4/IRAK1 ... demonstrated to inactivate the cellular protein toll-interleukin-1 receptor domain-containing adaptor inducing IFN (TRIF) TRIF is an adaptor protein that is a critical component of the TLR3 signaling pathway ... cells [76] In animal models Chemopreventive agents in preclinical development stage include S-adenosyl-L-methionine [77], curcumin [78], a 5a-reductase inhibitor [79], vitamin E [80], vitamin D [81],...

Ngày tải lên: 18/06/2014, 16:20

... reductions in VEGF-C In contrast, sunitinib-induced increases in VEGF-A were reduced in patients with high baseline VEGF-C at some time points, suggesting an attenuated hypoxic response in this ... of Atrium Inc., owns stock in Pfizer Inc., and was a paid contractor to Pfizer Inc in the development of this manuscript and the analysis and interpretation of data involving circulating biomarkers ... Dreyer C, Sablin MP, Serrate C, Cheng AL, Lanzalone S, Lin X, Lechuga MJ, Raymond E: Changes in Tumor Density in Patients with Advanced Hepatocellular Carcinoma Treated with Sunitinib Clin Cancer...

Ngày tải lên: 18/06/2014, 22:20

... reductions in VEGF-C In contrast, sunitinib-induced increases in VEGF-A were reduced in patients with high baseline VEGF-C at some time points, suggesting an attenuated hypoxic response in this ... of Atrium Inc., owns stock in Pfizer Inc., and was a paid contractor to Pfizer Inc in the development of this manuscript and the analysis and interpretation of data involving circulating biomarkers ... Dreyer C, Sablin MP, Serrate C, Cheng AL, Lanzalone S, Lin X, Lechuga MJ, Raymond E: Changes in Tumor Density in Patients with Advanced Hepatocellular Carcinoma Treated with Sunitinib Clin Cancer...

Ngày tải lên: 20/06/2014, 04:20

... HCC = hepatocellular carcinoma; B-HCC = hepatitis B-related hepatocellular carcinoma; C-HCC = hepatitis C-related hepatocellular carcinoma; NBNC-HCC = no infection of HBV or HCV related hepatocellular ... = confidence interval; HCC = hepatocellular carcinoma; B-HCC = hepatitis B-related hepatocellular carcinoma; C-HCC = hepatitis C-related hepatocellular carcinoma; NBNC-HCC = no infection of HBV ... or HCV related hepatocellular carcinoma; AFP = alpha fetoprotein; ALT = alanine aminotransferase; AST = aspartate aminotransferase; T-Bil = total bilirubin; ICG R15 = indocyanine green retention...

Ngày tải lên: 09/08/2014, 02:21

... the first time in the literature The finding suggests that there is direct spillage of HCC cells into the systemic circulation via hepatic vein INTRODUCTION Hepatocellular carcinoma (HCC) is ... extrahepatic and intrahepatic recurrence, even in cases within the Milan criteria and without microscopic hepatic venous invasion COMPETING INTERESTS The authors declare that they have no competing interests ... metastasis of human hepatocellular carcinoma Hepatogastroenterology 2004, 51:1121-1123 13 Endo K, Terada T: Protein expression of CD44 (standard and variant isoforms) in hepatocellular carcinoma: relationships...

Ngày tải lên: 09/08/2014, 02:21