monitoring of subvisible particles in therapeutic proteins

... the lack of consensus on the definition of these proteins In this article, we focus on three classes of complex-forming proteins of related function, also including anchor and docking proteins The ... regions are often directly involved in protein–protein interactions [42], these points help us to interpret the possible role of disorder in Caskin1 and in other scaffold proteins To obtain a balanced ... composed of several hundred proteins, including receptors and channels, cell adhesion proteins, cytoskeletal proteins, G -proteins and their modulators, and signalling molecules including kinases...

Ngày tải lên: 16/03/2014, 02:20

... boiling incipience and is characterized by individual bubbles departing the surface without interference from surrounding bubbles As heat flux increases, the bubble frequency increases inducing ... with the existing techniques for enhancing heat transfer, the nanofluids show a great potential in increasing heat transfer rates in a variety of application cases, with incurring either little ... capable of producing mechanical bridging between particles All inorganic particles assume a charge when dispersed in water In the case of silica, this is due to surface silanol (Si-OH) groups losing...

Ngày tải lên: 07/04/2014, 23:05

... analysers (DTA) Using DTA equipment (TA-Inst 2100), samples were held in a Pt crucible and analysed against a calcined Al2O3 reference at a heating rate of 20 °C min-1.The nano -particles in aqueous ... luminescent signal includes emission from RE ions in glass host and fluoride nanoparticles at luminescent spectra In GCs, most of RE ions are incorporated in the fluoride crystal phase, which mainly ... bio-distribution of the drug The nanoparticles in aqueous solution from the GC-host also apply in non-invasive imaging by detecting the luminescence of nano -particles inside biological systems, like...

Ngày tải lên: 22/06/2014, 01:20

... media Methods of calculating the influence of parafin concentration to the sedimentation of particles are same to the one were shown in [1] The coefficient of the parafin amount in the Richardson ... sedimentation of multi- dispersed particles The application of the proposed method to other sources of crude oil need further investigations Table 1: Sedimentation speed of a particle in a liquid medium of ... thc nghim REFERENCES [1] Phan Dinh Tuan, Ta Dang Khoa, Modeling of the settlement of dispersed particles in the petroleum media with different viscosity, Proceeding of the Asian RSCE, Hanoi (2005)...

Ngày tải lên: 22/07/2014, 02:20

... analysis of the relationship between GP of the b2 receptor and clinical outcomes in a placebo-controlled, crossover trial examining the use of regularly scheduled albuterol or salmeterol in a group of ... biologic actions of corticosteroids.18 This was accomplished by retrospectively examining three independent clinical trials using ICS as the primary therapeutic intervention Variation in one gene, ... evolving science of asthma, we must continue to follow the very exciting literature regarding potential phenotypic and genotypic indicators of response to therapy because one or the combination of...

Ngày tải lên: 08/08/2014, 21:20

... domain gain in animals and there are also indications of the importance of adjacent gene joining [11] and NAHR [43] in the formation of chimeric genes in the Drosophila lineage Terminal gains of ... domain has lost the introns present in the original sequence and the pre-SET domain has an intron containing repeat elements in a position not present in the original domain, suggesting it was inserted ... events according to the position of domain insertion and number of exons gained Gains at amino and carboxyl termini and in the middle of proteins are shown separately The first column in each group...

Ngày tải lên: 09/08/2014, 20:22

... interval = 30 seconds, and length of the experiment = 90 minutes To evaluate the constancy in time of the recordings of each device, we considered the recordings as consisting of three 30- minute ... • locomotion (walking, running, jumping); • climbing (hanging or climbing on the bars of the cage, with two or four paws); Table 1: Means of the three parts of the recording interval and statistical ... the radar device Observer radar7 LOCOMOTION CLIMBING STRETCHING DIGGING RISING/LOWERING TURNING BITING DRINKING/EATING GROOMING SCRATCHING HEAD MOVEMENTS Observer radar7 Observer radar6 Mean 100%...

Ngày tải lên: 10/08/2014, 09:20

... with continuous EEG and BIS monitoring to determine the safety and efficacy of barbiturate-induced comas in children Competing interests The authors declare that they have no competing interests ... with continuous EEG and BIS monitoring Key messages • The BIS monitor provides continuous data on EEG suppression and potentially assists in the monitoring of barbiturate-induced coma in children ... may not be reliable and continuous full-channel EEG monitoring is not feasible in many centres, as in ours Hence, monitoring of a barbiturate coma using the Bispectral™ index (BIS™; Aspect Medical...

Ngày tải lên: 13/08/2014, 08:20

... Brownian particles in uence the performance of a fluctuating ratchet For this purpose, we perform Brownian dynamics simulations of particles that move in a toroidal trap under the in uence of a sawtooth ... φ(t) of N = 20 particles in the toroidal trap The boxes indicate close-ups of the trajectories in panels (b) and (c) 145 Transient cluster formation of a pair of particles ... implication of ratchet theory on the description of molecular motors has been recognized [4, 60, 101] Molecular motors, e.g., kinesin proteins carrying cargo along tubulin filaments within cells,...

Ngày tải lên: 10/09/2015, 15:51

... account of discourse particles in Singapore English requires an account of the cognitive processes involved in interpreting them in discourse In the next section, I place the interpretation of discourse ... one I was thinking of doing like end of next week ah B: Uhm Okay end of next week A: Okay lah So we talk again tonight lah B: Okay (ICE-SIN-S1A-091)13 There is a generous sprinkling of particles ... presents an in- depth account of the discourse particles of a contact variety of English in Singapore The discourse particles, the elements (in italics) in the following [3], have been the focus of studies...

Ngày tải lên: 15/09/2015, 21:58

... Strathclyde, Scotland nd A THESIS SUBMITTED FOR THE DEGREE OF MASTERS OF ENGINEERING DIVISION OF BIOENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2008 ii DEDICATION I would like to dedicate this ... LOADING AND UNLOADING OF MZ CHANNEL 71 FIGURE 3-41 : INCLINOMETERS CALIBRATION AT ZERO 72 FIGURE 3-42: SAGGITAL PLANE INCLINOMETER CALIBRATION 73 FIGURE 3-43: INCLINOMETER ... slide is in the plane of interest An alignment reading was then performed by sliding the forks of the frame between the lower pair of wedges on the leg The pointer was then pushed onto the pointer...

Ngày tải lên: 05/10/2015, 19:01

... Continuous internal integrity monitoring and annual external integrity testing Monitoring Analyze injectant; continuously monitor annular pressure; monitor for fluid movement into USDWs within ... Addressing the Objectives and Goals of Monitoring 5.0 Addressing the Objectives and Goals of Monitoring The principal goal of DOE’s Carbon Sequestration Program is to gain scientific understanding of ... Developing and assessing the effectiveness of existing and novel monitoring tools • Assessing how these monitoring tools can be used efficiently, effectively, and hierarchically in a mature monitoring...

Ngày tải lên: 18/02/2014, 01:20

... were combined and yielded nucleosolic proteins The remaining pellet contains all DNA and structure bound proteins and is further referred to as chromatin-fraction Electrophoresis of proteins and ... BrdU staining and this again signifies that PCNA is only bound to chromatin portions where actively replicating DNA is present Recruitment of proteins involved in replication to chromatin during the ... by cells entering S-phase in succession Separating a cell fraction containing DNA bound proteins Entire replicative SV40 minichromosomes bearing functionally bound replication proteins can be eluted...

Ngày tải lên: 21/02/2014, 00:20

... containing sulfated sialyl-Lewis X like molecules Inhibiting leukocyte rolling by blocking selectins affects the accumulation of leukocytes in many experimental settings [14,15] Blocking selectin ... S1PR5 Integrins Integrins constitute a family of 24 heterodimers with aand b-subunits whose ligand-binding activity is regulated 4420 Blocking antibody Blocking antibody Preclinical Preclinical ... effects in a phase II clinical trial in stroke patients [24] These results dampened the enthusiasm for targeting integrin function in ischemic settings However, in inflammatory diseases, the inhibition...

Ngày tải lên: 07/03/2014, 12:20

... C2 domains were not directly adjacent but were joined by a linker sequence The intracellular kinase domain conforms to the canonical sequence of serine ⁄ threonine protein kinase domains seen ... Cg-TGFbsfR2 is shown in Fig 2C The serine ⁄ threonine kinase domain in both proteins is encoded by two exons equivalent to kinase subdomains X and XI [22] In addition, the GS box and L45 loop of Cg-BMPR1, ... the binding properties of each of these domains we generated synthetic mRNA encoding only the C1 or C2 domains Expression of only the C1 domain resulted in the dorsalization of embryos in a concentration-dependent...

Ngày tải lên: 07/03/2014, 21:20

... on the Clinical Investigation of the Pharmacokinetics of Therapeutic Proteins (CHMP/EWP/89249/2004) • Clinical Investigation (CPMP/BPWG/198/95) • Clinical Investigation of Recombinant Factor VIII ... addressed after the implementation of the change in a post-marketing setting 4.5.4 Immunogenicity in paediatric indications Therapeutic proteins are increasingly used in children It has to be considered ... sulfatation during all steps of the manufacturing process and during storage Fusion proteins composed of a foreign and self-protein are of particular concern because of the potential of the foreign...

Ngày tải lên: 13/03/2014, 21:55

... diagnosis of TB in the elderly A positive sputum AFB smear was obtained in 57.1% of elderly and in 57.5 % of young TB patients, and a positive AFB culture was obtained in 70.6% of the elderly and in ... data included the following: demographic characteristics, presenting symptoms and body temperature, underlying illnesses, laboratory findings (including microbiologic study at the time of diagnosis), ... challenge of tuberculosis of the elderly in hospital: experience at a university hospital in Saudi Arabia J Infect 1995; 31: 93-7 22 Morris CD Sputum examination in the screening and diagnosis of pulmonary...

Ngày tải lên: 15/03/2014, 03:20

... surface remnants of triglyceride-rich lipoproteins Arterioscler Thromb 14, 622–635 Pal S, Semorine K, Watts GF & Mamo J (2003) Identification of lipoproteins of intestinal origin in human atherosclerotic ... following the manufacturer’s instructions 5638 Culture of THP-1 cells and experimental protocol THP-1 monocytes were maintained in suspension in RPMI 1640 culture medium containing 10% fetal bovine ... bind to the LDL receptor family in its lipid-free state The receptorbinding region is in an amphipathic a-helix which is part of a four-helix bundle structure in the N-terminal domain, and interaction...

Ngày tải lên: 16/03/2014, 12:20

... indicated protein or EZ-Link-sulfo-NHS-LC-biotin labelling by SDS ⁄ PAGE and immunoblotting or avidin-labelled HRP, respectively The amounts of indicated proteins are expressed in arbitrary densitometric ... normalized to percentage of maximum (A) Protein concentration (mgÆmL)1) (B) Caveolin-1 (C) Caveolin-2 (D) Cell surface biotin labelling of proteins (E) TGN38 (F) Insulin receptor (G) GLUT4 (H) ... referring to all of them as caveolae, was demonstrated by their content of both caveolin-1 and caveolin-2 The coexistence of caveolin-1 and caveolin-2 in all three caveolae subclasses is in line...

Ngày tải lên: 16/03/2014, 13:20

... A2 mature proteins each contain a single methionine towards the C-terminus of the peptide (shown underlined and italicized) The methionine at the end of A2 was substituted with leucine in both ... group of thylakoid proteins, it is now very interesting to compare and contrast their insertion mechanism with that of procoat Initial models for the insertion of these thylakoid proteins were ... used for the targeting of thylakoid proteins in chloroplasts Traffic 2, 245–251 Lamppa, G.K (1988) The chlorophyll a/b-binding protein inserts into the thylakoids independent of its cognate transit...

Ngày tải lên: 18/03/2014, 01:20