molecular structure and biological activity

... of hCD2 and rat CD2 3D structures indicated that Tyr86 and Glu36 side chains overlap in hCD2 and rat CD2 and oriented in the same direction in both the structures To stabilize the b-turn structure ... Human-CD2 and rat-CD2 have high 3D structure similarity Comparison of 3D structures of hCD2 from CD2–CD58 complex [30] and the structure of rat CD2 [36] using DS modeling (Accelrys Inc., Sandiego, ... N- and C-termini A comparison of the b-turn structure of cER with the similar region in the crystal structure of rat and human CD2 was carried out In the rat CD2 crystal structure, the b-turn structure...

Ngày tải lên: 19/02/2014, 13:20

... virus–cell interactions, and to find permissive cell lines for further molecular and cell biological studies of this peculiar human virus For this purpose, we have cloned and sequenced the full-length ... emerged in 293T, Huh7 and UT7 ⁄ Epo-S1 cells, and less pronounced also in 293 and Chang liver cells (Fig 3A,C, marked with an arrow) However, no such bands appeared in KU812Ep6 and Cos-1 cells That ... information on the molecular biology and host–cell interactions of this virus is needed To this end, we have cloned and sequenced the full-length genome of TTV genotype 6, and studied the key biological...

Ngày tải lên: 16/03/2014, 05:20

... MOLECULAR MECHANISMS AND BIOLOGICAL EFFECTS Edited by Volodymyr Lushchak and Halyna M Semchyshyn Oxidative Stress Molecular Mechanisms and Biological Effects Edited by Volodymyr Lushchak and ... Semchyshyn and Volodymyr I Lushchak Chapter Oxidative and Nitrosative Stresses: Their Role in Health and Disease in Man and Birds 47 Hillar Klandorf and Knox Van Dyke Chapter Nitric Oxide Synthase and ... mutagenic and teratogenic actions of RCS and ROS, and products of these interations have powerful Interplay Between Oxidative and Carbonyl Stresses: Molecular Mechanisms, Biological Effects and Therapeutic...

Ngày tải lên: 12/02/2014, 16:20

... monosaccharides present in the products and were identified, and the ratio of structures and was close to : All chemical shifts remained mostly unchanged with the exception of H/C-2 and H/C-3 of the glycerol ... together, allow us to propose the structures of oligosaccharides and 2, as presented in Scheme The oligosaccharides were further analyzed by CE-MS and by CE-MS/MS (Figs and 6) The mass spectra obtained ... Ac2O in pyridine (0.5 + 0.5 mL, at 85 °C for 30 min), dried and analyzed by GC-MS with the standards prepared from D-GalN and (R)- and (S)-2-BuOH The absolute configuration of L-aspartic acid...

Ngày tải lên: 19/02/2014, 16:20

... sera to a 9- and a 15-kDa band We could show that the 9-kDa band in the tomato extracts reacts with a specific antibody against the LTP from cherry, Pru av and the 15-kDa band shows reactivity using ... difference in IgE reactivity to glycoproteins between both groups Besides binding to protein bands larger than 20 kDa we also observed reactivity to proteins with a molecular mass of 15 and kDa IgE binding ... tomato-allergic patients (A, B) Patient 1, showing IgE reactivity to nLyc e and CCD (C,D) Patient 2, showing no IgE reactivity to nLyc e and CCD Standard deviations are shown for each measurement horseradish...

Ngày tải lên: 21/02/2014, 00:20

... structures and molecular shape For the determination of the three-dimensional supramolecular structure of the lipid aggregates, X-ray diffraction measurements were performed at the European Molecular ... cm)1 and ms (PO2–) at 1120 cm)1, respectively [23,30], and the bands at Fig Infrared spectrum in the spectral range 1800–900 cm)1 (A) and – in the range of the negatively charged phosphate band ... cross-sections of the hydrophilic and hydrophobic moieties are identical, and conical or wedgeshaped in the case of nonlamellar cubic and direct HI or inverted HII structures; the cross-sections...

Ngày tải lên: 21/02/2014, 00:20

... comparative analysis of Ngb and Mb may be used to better define the relationships between these two globins and to obtain a better understanding of the molecular features and biological function of ... neutral and acidic pH conditions by a variety of spectroscopic (i.e CD, fluorescence and second-derivative UV absorption) and biochemical (i.e limited proteolysis and ligand binding) techniques, and ... crystallographic structure of Ngb (Protein Data Bank 1OJ6: chains B and C) [3] In fact, only Tyr137 is located in a buried and hydrophobic site; Tyr88 and Tyr115 are highly exposed on the protein surface, and...

Ngày tải lên: 07/03/2014, 02:20

... ‘open’ (standard conformation; PDB 1YC0) and ‘closed’ (nonstandard conformation; PDB 1YBW) HGFA forms are superimposed, with the two different 220-loop conformations shown in cyan and magenta, ... (standard) active site conformation The determined HGFA structures reveal three different conformational states of the active site region: a catalytically competent (standard) conformation, and ... of the catalytically competent structure are the ‘oxyanion hole’ formed by the amide nitrogens of Ser195 and Gly193, and substrate-binding subsites (S1, S2, S3, and S4), which interact with the...

Ngày tải lên: 15/03/2014, 11:20

... 0.5 p.s.i and the separations were performed at 650 VÆcm)1 and 25 °C The peaks of the references appeared after 1.8 (I), 2.1 (e-fluoresceinyl lysine) and 4.2 (II) Apart from free I and II, the ... FACS and CLSM by providing absolute quantitation of internalized PNA CLSM revealed extensive fluorescence in the cytosol and nucleus of CM and CHO cells after exposure to both the naked PNA I and ... (trypan blue) and emission was measured at 515 nm and 570 nm, respectively Three regions of interest (16 · 16 pixel; 30 scans with a scan time of s with double averaging) in the cytosol and one in...

Ngày tải lên: 23/03/2014, 13:20

... Hemoglobin and Myoglobin and Their Reactions with Ligands Elsevier, North-Holland Publishing, Amsterdam, the Netherlands Dickerson, R.E & Geis, I (1983) Hemoglobin: Structure, Function, Evolution and ... in the crystal structure of Pe metHbH2O [16] and as conrmed herein by solution 1H NMR of Pe metMbCN Proximal and distal residues are shown as rectangles and circles, respectively, and arrows connecting ... ligand to participate in H-bonding to the ligated cyanide The van der Waals surfaces for the Tyr32(B10) Og and the 10 tilted cyanide ligand are shown in Fig and establish that the Tyr Og and...

Ngày tải lên: 23/03/2014, 17:22

... NMR structure determination (file parallhdg.pro and topallhdg.pro) was used, and an initial structure was built by randomly generating F and C angles In the calculations starting from random structure, ... agonists and antagonists of ODN We have determined the secondary structure of the cyclic OP analogs by two-dimensional 1H-NMR and molecular dynamic simulation, and we have investigated the biological ... Ha-Leu5 and NH-Leu7, Ha-Leu6 and NH-Lys8, in combination with the coupling constants and slow H/D exchange of NH-Leu4, NH-Lys8, suggested a first turn centered on the Gly3 and Leu4 residues, and a...

Ngày tải lên: 24/03/2014, 04:21

... previously [15], the 49 accepted structures were refined, and finally 20 structures with the lowest energies were chosen to represent the solution structure RESULTS AND DISCUSSION Biological activities of ... antimicrobial activity against prokaryotic cells, but very little hemolytic Ó FEBS 2002 Structure activity relationships of GGN4 analogues (Eur J Biochem 269) 4369 Table Antimicrobial activity (a) and ... including the native GGN4, showed a strong negative band near 200 nm and a weak and broad band around 222 nm, indicating a predominantly random-coil conformation with a slight helical propensity...

Ngày tải lên: 31/03/2014, 09:20

... ml of saline (for virus control and cell control) and given ml 2x MEM The cells were incubated for 72 hours and observed for cytopathic effects from the virus and cytotoxic effects from the samples ... heptaphylla in high yield (0.7 ÷ 0.8%) The structure of the compound was determined by interpretation of its spectral data (IR, MS, 1H and 13C-NMR) and by comparison with spectral data of an ... Dr Tran Ngoc Ninh, Vietnamese Academy of Science and Technology Extraction and isolation Leaves of Clausena heptaphylla were dried at 40 ÷ 500C and powdered The powder (500 g) was extracted with...

Ngày tải lên: 03/04/2014, 15:20

... 2 EURASIP Journal on Bioinformatics and Systems Biology Three contributions are concerned about how network structure in general determines the dynamics of molecular profiles they regulate Rosenfeld ... approach for improved reverse engineering Specific biological questions were in focus of three articles: Andorf et al investigated a systems biological hypothesis towards explaining heterosis ... series profiles from heterozygote crossings and make further predictions regarding expected heterosis effects if very different lines are crossed Ebenhoeh and Handorf describe two functional classifications...

Ngày tải lên: 22/06/2014, 00:20

... Semchyshyn and Volodymyr I Lushchak Chapter Oxidative and Nitrosative Stresses: Their Role in Health and Disease in Man and Birds 47 Hillar Klandorf and Knox Van Dyke Chapter Nitric Oxide Synthase and ... mutagenic and teratogenic actions of RCS and ROS, and products of these interations have powerful Interplay Between Oxidative and Carbonyl Stresses: Molecular Mechanisms, Biological Effects and Therapeutic ... other hand, due to high reactivity RCS interact with different cellular constituents that may contribute to aging, age-related diseases, and diverse metabolic disorders 2.1 Structure and reactivity...

Ngày tải lên: 28/06/2014, 11:20

... trees Holuša and Vidlička (2002) observed the peak of its activity in spruce forests of Paskovský les and Loucký les at the end of May in 1998 The delay of its flight activity in highlands, compared ... Albrechtice and Skřipov (18, resp 17 species of Neuroptera) and a slightly lower number was found in the village Podvihov (14 species) The second group includes the localities Kyjovice, Pustá Polom and ... drought and infestation by honey fungus By comparison, other localities are covered with much closer and denser spruce forests as in the surroundings of Skřipov Localities Lesní Albrechtice and Skřipov...

Ngày tải lên: 07/08/2014, 03:22

... lignin structure and monomeric composition have indeed been found and confirmed between plant species (Logan and Thomas, 1985), between plant organs and tissues grown either in vitro or in vivo and ... repetitive and more flexible structures, such as macromolecular systems, can be self- lignin macromolecules, explaining their functional fitness and the biological significance of the ’random process’ ... monocotyledons (grasses and bamboos) and Salicaceae (poplars) Ester bonds of phenolic acids to C and Cy-hydroxyls of a monomeric propane chains (Fig 1C5to Molecular associations and cell wall lignification...

Ngày tải lên: 09/08/2014, 03:24

... aggregation properties of CPP activity and supramolecular structure Table Synthetic CPP primary structures The ‘cluster sequence’ characteristic of all CPPs is underlined and indicated in bold characters ... compilation ª 2007 FEBS 5005 CPP activity and supramolecular structure C Gravaghi et al of the biologically active aggregates, the simultaneous presence of CPP and Ca2+ is needed while complexes ... derived from sedimentation equilibrium and small-angle X-ray and neutron-scattering measurements Eur J Biochem 252, 73–78 CPP activity and supramolecular structure 17 Ferraretto A, Signorile A,...

Ngày tải lên: 16/03/2014, 05:20

... learn and grow but also freedom to try and err He is always a good friend and true mentor of mine I appreciate his trust in me and his care and friendship I am grateful to his insight and advice ... (P-15), and REDV (Qian and Bhatnagar, 1996; Massia and Stark, 2001; Mann and West, 2002; Dhoot et al., 2004; Hanks and Atkinson, 2004; Boateng et al., 2005; Guler et al., 2006) 11 Chapter The molecular ... formation of intermolecular isopeptide ε-(γ-glutamyl)lysine crosslink (Lorand and Graham, 2003; Esposito and Caputo, 2004) 31 Figure 3.1 (a) Type I collagen comprises three left-handed helical polypeptide...

Ngày tải lên: 12/09/2015, 08:20

... nitrifying bacterial populations and the in situ activity of these populations within the biofilms was discussed Microsensors For analysis of microbial structure and function (activity) of such complex ... situ hybridization (FISH) and microsensor techniques have a high spatial (at a single-cell resolution) and temporal resolution and great potential, and provide reliable and direct information about ... and F J de Bruijn (ed.), Molecular microbial ecology manual Kluwer Academic Publishers, Dordrecht, The Netherlands Amann, R I., W Ludwig, and K -H Schleifer 1995 Phylogenetic identification and...

Ngày tải lên: 05/09/2013, 08:40