molecular organization in lipids and emulsions
Food emulsions, fourth edition
Ngày tải lên :
23/05/2014, 11:36
... Chemical and Physical Properties Niels J Krog and Flemming Vang Sparsø Molecular Organization in Lipids and Emulsions Ka˚re Larsson Interactions Between Proteins and Polar Lipids Tommy Nylander ... including photocopying, microfilming, and recording, or by any information storage and retrieval system, without permission in writing from the publisher Current printing (last digit): 10 PRINTED ... respective influence of interprotein and protein–solvent interactions Because in practice the protein is displaced from the interface in an aggregated form, the changes in the protein structures and...
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HCV functional genomic protein interactions with NS3 and their role in viral replication
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16/09/2015, 17:12
... protein containing the RNA-binding motif reduces the strength of the interaction The NS3-NS3 interaction was also confirmed by co-expressing myc-tagged and flag-tagged NS3 in COS-7 cells and showing ... implicating its involvement in oncogenesis Taken together, most of the viral proteins interact and may affect the activities of host proteins in the long-term The understanding of the interplay ... player in HCV replication, in the hope of providing new insights into understanding function and effect of these interactions Yeast-two hybrid screens were set up to identify HCV proteins as...
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Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
Ngày tải lên :
07/03/2014, 10:20
... decrease in the intrinsic fluorescence of GST–HIPPI protein, indicating binding of the protein with this mutated motif (Fig 1B, panel II) The apparent dissociation constant (Kd) of this binding was ... remains to be determined HIPPI does not have any similarity with known proteins having DNA-binding motifs However, it contains the pDED at the C-terminus (amino acid residues 335–426) and a myosin-like ... hydrophobic ones in the later [8] DED-containing proteins are known to participate in diverse cellular functions, including apoptosis through receptor signaling [20,21] Other DED-containing proteins, such...
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
Ngày tải lên :
07/03/2014, 17:20
... obtained in 10 mm Tris ⁄ HCl buffer containing 0.15 m NaCl These results suggest that electrostatic interactions play a principal role in the binding of E2(7–26) to negatively charged residues In ... from Fluka (Bucks, Switzerland) Rink amide MBHA and Novasyn TGR resins, amino-acid derivatives and coupling reagents were obtained from Fluka and Novabiochem (Nottingham, UK) Dimethylformamide ... element containing amino acids 98–110 of the flavivirus E protein) may serve as an internal fusion peptide, as it is directly involved in interactions with target membranes during the initial stages...
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Báo cáo khoa học: Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1 doc
Ngày tải lên :
16/03/2014, 05:20
... of integrins requires talin binding to the cytoplasmic tail of b integrin [13] Talin is an essential integrin-activating protein that connects the cytoplasmic tail of b integrins to the actin ... domain fold masked by an extended actin binding tail domain Cell 101, 259–270 29 Balla T (2005) Inositol-lipid binding motifs: signal integrators through protein–lipid and protein–protein interactions ... with FERM (band four-point-one ⁄ ezrin ⁄ radixin ⁄ moesin) domains FERM domains localize proteins to the plasma membrane, where they can interact with phosphoinositides and membrane proteins [21]...
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Báo cáo khoa học: Cdc37 maintains cellular viability in Schizosaccharomyces pombe independently of interactions with heat-shock protein 90 doc
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16/03/2014, 22:20
... the middle and 4132 all of the C-terminal domains, including the postulated Hsp90-binding and dimerization regions [16] Mutants with truncations extending into the N-terminal domain, Cdc37(1–155), ... in- frame insertions resulting in the insertion of five amino acids into the Cdc37 protein Stop codons were inserted by the five codon insertion in four mutants, each adding only 1–3 extra amino acids ... protein may act inefficiently in S pombe blot analysis on GST, S pombe protein extracts and GST-Cdc37 using depleted serum results in a loss of signal against S pombe protein extracts and recombinant...
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Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
Ngày tải lên :
17/03/2014, 10:20
... Kruijff, B (1998) An investigation into the lipid interactions of peptides corresponding to the C-terminal anchoring domains of Escherichia coli penicillinbinding proteins 4, and Biochim Biophys ... representing these sequences lie proximal in the area delineating candidate oblique-orientated a-helix-forming segments, indicating that m-calpain domain V may contain an oblique-orientated a-helix comparable ... of band components was obtained from deconvolution of the spectra; curve fitting was then applied within the original spectra after the subtraction of baselines resulting from neighbouring bands...
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Báo cáo khoa học: Deciphering the key residues in Plasmodium falciparum b-ketoacyl acyl carrier protein reductase responsible for interactions with Plasmodium falciparum acyl carrier protein pptx
Ngày tải lên :
23/03/2014, 10:20
... mutating the corresponding residues in E coli FabG involved in the binding of E coli ACP have been investigated earlier, the number of binding sites and ACP-induced cooperativity for the interactions ... study ligand-induced conformational changes by monitoring the change in its intrinsic fluorescence In these studies, we have investigated the interactions between PfFabG and PfACP using a combination ... concentrations The apparent binding and association constants are shown in Table There was a three-fold enhancement in PfACP binding to wild-type PfFabG in the presence of NADPH PfACP binding to the mutant...
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Báo cáo khoa học: Interaction of ostreolysin, a cytolytic protein from the edible mushroom Pleurotus ostreatus, with lipid membranes and modulation by lysophospholipids pptx
Ngày tải lên :
23/03/2014, 21:20
... structural changes in ostreolysin on binding to SEL SUVs, but little or no binding with LUVs containing 10% lyso-PtdIns The collective results (Figs and 8, and Table 2) suggest that binding to the lipid ... lyso-PtdIns for 20 before adding the LUVs or preincubating LUVs and then adding ostreolysin Fluorescence measurements Steady-state intrinsic uorescence of ostreolysin, either alone or in combination ... to the protein and inactivate it The observation that lysophospholipids, either alone or in combination with other lipids (Fig 5), can inhibit ostreolysin hemolytic and permeabilizing activity,...
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Báo cáo Y học: Secretion of a peripheral membrane protein, MFG-E8, as a complex with membrane vesicles A possible role in membrane secretion pptx
Ngày tải lên :
24/03/2014, 03:21
... for intracellular staining After blocking with NaCl/Pi containing 2% BSA (blocking solution) for 30 min, the specimens were incubated for 60 with the rabbit antiserum raised against the recombinant ... vesicles showed the PtdSer binding ability (Fig 5), indicating that only C2 domain was required and enough for binding to PtdSer coated on the plate This binding by C2 domain alone might be due to ... In fact, the DC1 protein had the PtdSer-binding ability probably through the remaining C2 domain regarded as a phospholipid-binding domain Consequently, the membrane association of MFG-E8-L and...
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Báo cáo khoa học: Interactions of the antimicrobial b-peptide b-17 with phospholipid vesicles differ from membrane interactions of magainins potx
Ngày tải lên :
31/03/2014, 07:20
... b-17 and magainin II or [Ala8,13,18]-magainin II amide in the way these antimicrobial peptides interact with vesicle systems Ó FEBS 2003 While both magainin II [29] and [Ala8,13,18]-magainin II ... magainin analog, [Ala8,13,18]-magainin II amide [30] We find that the peptide-induced changes in the transition of DPam2PtdEtn are similar for both magainin II and for [Ala8,13,18]-magainin II ... with liposomes containing PtdGro than with liposomes containing PtdSer [29] We found that b-17 (Fig 6) did not discriminate, in terms of inducing leakage, between vesicles containing Ole2PtdGro or...
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A validated molecular docking study of lipid–protein interactions
Ngày tải lên :
28/11/2015, 14:01
... protein and ligand, the ligand binding pocket of the proteins, the active site amino acids of the protein which interact with the ligand, the intermolecular interactions between the ligand and ... the binding affinity, the ligand binding site of protein, the amino acids of the protein interacting with ligand, PDB files1 of protein bound to ligand etc) of all the 80 lipid-protein interactions ... binding affinity between them In other words binding affinity or binding energy is the direct measure of the strength of binding between ligand and receptor Furthermore, the binding affinity between...
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Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
Ngày tải lên :
14/02/2014, 14:20
... Proteins harbouring SH2 domains bind to phosphorylated tyrosines on their target proteins, thereby coupling the activity of tyrosine kinases with intracellular signalling pathways [28] In our ... results in significantly increased binding of tau to Fyn-SH2, thus suggesting that the interaction between tau and Fyn-SH2 is enhanced by increased tyrosine phosphorylation in cells To determine whether ... tyrosines present on tau [17–19], and tau also interacts with Fyn via its Src homology (SH)3 domain [20,21] The SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting...
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Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx
Ngày tải lên :
14/02/2014, 19:20
... RNA–protein interactions in RNA folding M Doetsch et al Tat peptide induced a change in the population of coexisting and interchanging RNA conformations The lack of intermolecular NOE connectivities indicated ... helicases The binding sites for doublestranded RNA and ATP overlap, resulting in coupled binding of both molecules Simultaneous binding forces the RNA into a bent conformation that is incompatible ... describing macromolecular complexes is by the molecular interface of the interacting molecules In common stable complexes between RNAs and their specific RNA-binding proteins, such as the RRM domains...
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Tài liệu Báo cáo khoa học: Transient DNA ⁄ RNA-protein interactions docx
Ngày tải lên :
14/02/2014, 19:20
... structure facilitating protein binding (indirect) and specific amino acid–base contacts (direct) A recent examination of proteinÆnucleic acid structures has shown how minor groove narrowing enhances ... kdt,CB B Fig Proteins find their binding sites in an ocean of DNA sequences Scheme of a nucleic acid-binding protein (gray ellipses) involved in four dynamic equilibria (double arrows) and one-dimensional ... of large proteins, and the simplification of the system to facilitate crystallization and ⁄ or analysis by other techniques However, investing time and effort in preparing and analyzing the full-length...
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Tài liệu The Acquisition of Drugs and Biologics for Chemical and Biological Warfare Defense - Department of Defense Interactions with the Food and Drug Administration doc
Ngày tải lên :
17/02/2014, 11:20
... Second, industry makes a deep investment in education and training (E&T) of its personnel in manufacturing At Merck Manufacturing Division, for example, formal training includes those who handle ... development and licensing capability Were DoD more effective in managing the drug development and licensing process, fewer candidate drugs and vaccines would be “parked” in IND status, and more ... drugs and vaccines depends in large measure on DoD’s understanding of the regulatory requirements of FDA and on incorporating this knowledge into its own policies, organization, budgets, and procedures...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
Ngày tải lên :
18/02/2014, 08:20
... of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark ... methylcob(III)alamin to homocysteine, generating cob(I)alamin and methionine A methyl group is then abstracted by cob(I)alamin from CH3-H4-folate, generating H4-folate and the methylcob(III)alamin form ... (light grey) and the C-terminal activation of hMS (grid-barrel) interact with the cobalamin-binding domain For more information on hMS conformational substates, see [5] and [33] cob(II)alamin], an...
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Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx
Ngày tải lên :
19/02/2014, 05:20
... phenotype, whereas the YL7 strains expressing either the LexA DNA binding domain alone or this domain fused to lamin, were not able to grow on a medium lacking histidine and did not show the b-galactosidase ... C-terminus of both isoforms of PDIP46 ⁄ SKAR, including the amino acids of the RNA recognition motif (RRM) also known as the RNA binding domain (Fig 2) [26] Interaction of the human proteins ER and ... this kinase (Fig 4D) [28] B C D Fig Interaction between ER and PDIP46 ⁄ SKAR examined by the GST pull-down assays (A) Proteins used in the assay with recombinant proteins expressed in E coli and...
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Tài liệu Báo cáo khoa học: Agrobacterium tumefaciens type II NADH dehydrogenase Characterization and interactions with bacterial and thylakoid membranes ppt
Ngày tải lên :
19/02/2014, 06:20
... observed in the absence of IPTG, likely indicating significant IPTG-independent expression of the protein Using an antibody raised against the recombinant protein, AtuNDH-2 was detected in protein extracts ... separation and fluorometric analysis of the flavinic cofactor Continuous line, cofactor extracted from purified recombinant AtuNDH-2; dashed black line, FAD standard; dashed grey line, FMN standard ... soluble protein fraction; M, membrane protein fraction AtuNDH-2 accumulation was analysed in response to induction by 0.1 or 0.5 mM IPTG Gel loading was 2.5 and 30 lg proteins in M and S lanes,...
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