molecular functions and its role in virus life cycle and pathogenesis

... proteins, Vpr, has been shown to dysregulate several host cellular functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins ... may play a role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream ... containing multiple ankyrin repeat motif domains and a single KH domain similar to the MASK gene found in Drosophila Drosophila MASK (dMASK) has been implicated in cell survival and may play a role...

Ngày tải lên: 07/03/2014, 21:20

... very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... the structural and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 domain (B) KIND2 domain anchors v-KIND to dendrites Flag-tagged v-KIND, DKIND1, DKIND2, DKIND1 + 2, DRasN, DGEF, KIND1 or KIND2 together with...

Ngày tải lên: 14/02/2014, 19:20

... wild-type occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, we revealed that exon played a major role in the induction of mitochondria-mediated apoptosis and the ... occludin in apoptosis and invasion A C B D E F Fig Identification of a human occludin splice variant in liver cell lines (A) Occludin protein expression was examined with an antibody to the N-terminus ... cells In Chang cells, stained occludin was observed in the cytoplasmic region Additionally, in OccWT-overexpressing Hep3B and Huh7 cells, regions stained with antibody to hemagglutinin (HA), indicating...

Ngày tải lên: 18/02/2014, 18:20

... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms ... C-terminus (box in Fig 1) at sites: 92, 75, 66 and 58 As histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although ... binding on the secondary structure was determined using CD in the far UV To prepare proteins without Cu2+, part of each protein 4252 Fig Comparison of copper binding by stefin A and stefin B using...

Ngày tải lên: 19/02/2014, 06:20

... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay ... Characterisation and mitochondrial localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase Gene 228, 85–91 Nakagawa J & Moroni C (1997) A 20-amino-acid autonomous RNA-binding domain contained in...

Ngày tải lên: 19/02/2014, 07:20

... (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations ... were removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation ... were incubated at 37 °C in a humidified 5% CO2 incubator in the absence (control) and presence of increasing concentrations of inhibitors for 10 days, and the number of molting larvae was determined...

Ngày tải lên: 20/02/2014, 11:20

... Printing Office, Washington, D.C., 2004 SOLAR AND SPACE PHYSICS AND ITS ROLE IN SPACE EXPLORATION the fundamental role of solar and space physics research both in scientific exploration and in ... principal operating agency of both the National Academy of Sciences and the National Academy of Engineering in providing services to the government, the public, and the scientific and engineering ... Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers It is autonomous in its administration and in the...

Ngày tải lên: 14/03/2014, 10:20

... discounted A Interest Rate and Business Investment The first step in examining how higher interestrates may influence business investmentdecisions and performances is the understandingof firms' ... Government plays a dominantrole in the financialmarket and its interventiinstake many forms, not only in terms of deficit financing,but also equity participationand management control in virtually all ... and domestic demand including public coneumption,the poor performance in export earnings brought about by a combinationof fallingoil prices, rising interestpaymentson external debt, and declining...

Ngày tải lên: 15/03/2014, 14:20

... no membrane-spanning domains in PEX14 have been unambiguously identified and it was observed in mammalian cells and S cerevisiae that both the N-terminal and the C-terminal domain are at the cytosolic ... synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The miscompartmentation of PTS-1 and PTS-2 proteins (GAPDH and ALD, ... approximately 0.5 to induce the expression of the protein and growth was continued for h Cells were collected by centrifugation (3000 g, 10 min, °C) and resuspended in cell lysis buffer containing 50 mM...

Ngày tải lên: 23/03/2014, 17:21

... staining The four bands in molecular weights of 20, 15, 13, and 11 kDa were cut out and sent for N-terminal sequencing The sequence data we obtained from N-terminal sequencing is represented in ... (FCS, Invitrogen), mM glutamine (Invitrogen), and 10 mM gentamicin sulfate (Invitrogen) at 37°C in a 95% humidified atmosphere containing 5% CO2 For infection of WR, BSC-40 cells were maintained in ... plays a role in the correct assembly of nucleoprotein complex and organization of IV content with the membranes while F17R (a DNA-binding phosphoprotein), and L4R (a DNA-binding protein) are...

Ngày tải lên: 18/06/2014, 18:20

... staining The four bands in molecular weights of 20, 15, 13, and 11 kDa were cut out and sent for N-terminal sequencing The sequence data we obtained from N-terminal sequencing is represented in ... (FCS, Invitrogen), mM glutamine (Invitrogen), and 10 mM gentamicin sulfate (Invitrogen) at 37°C in a 95% humidified atmosphere containing 5% CO2 For infection of WR, BSC-40 cells were maintained in ... plays a role in the correct assembly of nucleoprotein complex and organization of IV content with the membranes while F17R (a DNA-binding phosphoprotein), and L4R (a DNA-binding protein) are...

Ngày tải lên: 20/06/2014, 01:20

... Available online http://arthritis-research.com/content/6/1/26 known roles in tissue inflammation, and damage in innate immunity Besides its capacity to drive the production of IFN-γ and IL-2 by ... Stoll S, Germain RN: Imaging of T-cell interactions with antigen presenting cells in culture and in intact lymphoid tissue Immunol Rev 2002, 189:51-63 O’Sullivan B, Thomas R: CD40 and dendritic ... development of inflammatory arthritis [14-16] This highlights the critical role of IL-1ra in the constitutive maintenance of peripheral tolerance, and in counterbalancing the proinflammatory effects...

Ngày tải lên: 09/08/2014, 01:23

... loss during intestinal tumorigenesis may, in part, act through upregulation of b-catenin, increasing intestinal b-catenin signaling and cell proliferation Also, increase in small-intestinal mucosa ... regulating the circadian clock include acetylation, phosphorylation, ubiquitination and sumoylation In terms of phosphorylation, Casein kinase epsilon (CK1ε) and Casein kinase delta (CK1ε and CK1δ), ... repair is found to display prominent circadian oscillations in mouse brain reaching at its maximum in the afternoon/early evening hours and minimum in the night/early morning hours The circadian oscillation...

Ngày tải lên: 10/08/2014, 09:20

... those involved in insulin signaling, the insulin receptor substrate (IRS)-1, IRS2 and signal transducer and activator of transcription (Stat)-6 [23] IRS1 and IRS2 regulate proliferation and protection ... Acting as a decoy to bind and neutralize circulating cytokine, coupled with the high specificity and high affinity of binding for the cytokine, makes the soluble receptor ideal as a cytokine ... arthritis in adults and children Clinical studies with rhuIL-4R (Nuvance™) The promising results in preclinical studies led to preliminary investigations in which rhuIL-4R proved safe and effective in...

Ngày tải lên: 12/08/2014, 18:20

... also increasingly involved in the development and delivery of a range of education and training programmes designed to help individuals working in HIV advance professionally, from administering ... Providing education and training opportunities for its membership is important for any professional society, and the IAS is looking at ways to strategically expand its role in this area in order ... best practice and advocacy in the fight against HIV/AIDS The IAS is also strengthening its commitment to regional development, expanding our involvement in three main areas: collaboration with...

Ngày tải lên: 13/08/2014, 09:20

... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... molecular chaperones in the folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342 ... cytokines play a major role in sepsis-induced liver injury [19,20], we also investigated the expression of PDI in cells of the macrophage-like cell line, RAW 264.7, after incubation with LPS In...

Ngày tải lên: 13/08/2014, 11:22

... transduced into OV90 cells 103 xiv Figure 33 Wild-type TG2 and N-terminal fibronectin binding domain of TG2 induce EMT in OV90 cells Figure 34 104 Wild-type TG2 and N-terminal fibronectin binding domain ... at its protease sensitive hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase ... Tyr583 are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like growth...

Ngày tải lên: 24/08/2014, 10:58

... expressed predominantly in erythroid cells HRI is regulated by heme through the two heme-binding regions in HRI: an N-terminal domain of HRI and in an insert region in the protein kinase domain of HRI ... the initiation codon in underline and bold and a flanking purine at the -3 and a G at the +4 positions (15) Together the eIF1 and eIF1A facilitate the recognition and selection of initiating ... cytoplasmic splicing of mRNA encoding the Xbox binding protein1 (XBP1) in mammals and homologue of the mammalian activating transcription factor/cAMP-response element (CRE)-binding protein (HAC1) in yeast...

Ngày tải lên: 24/08/2014, 12:32

Ngày tải lên: 14/11/2014, 06:30

... DENV life cycle as host factors remain ill defined We show here that a functional UPP is critical for virus egress in both Aedes aegypti mosquitoes and human monocytic cell lines Using RNA inference ... Bortezomib inhibits infectious virus production for different strains of all dengue serotypes and YF17D in a dose-dependent manner in primary monocytes 105 Figure 3.19: Epoxomicin reduces infectious ... fever virus, Japanese encephalitis virus, West Nile virus and tick-borne encephalitis virus (Gould & Solomon, 2008) Flaviviruses are enveloped RNA viruses 45-50 nm in diameter that contain a singlestrand,...

Ngày tải lên: 09/09/2015, 08:20