molecular dynamics simulation studies of coupled protein and water dynamics

... II (PSII) of C reinhardtii [16] We now report the expression of His- and Strep-tagged plant PSI Results A series of cassettes containing tagged variants of the full-length precursor of Arabidopsis ... prepared from pools of plants expressing similar levels of PSI-G, and analysed by immunoblotting, using antibodies to PSI-G and PSI-F, as an indicator of the relative content of PSI in the samples ... Immunodetection of His and Strep tags in PSI-G constructs and transformed plants were treated separately with thermolysin and trypsin as described for the in vitro import analyses PSI-G, and a control protein, ...

Ngày tải lên: 07/03/2014, 21:20

... piles of cabbage and cauli-flowers, the rows of tin ware and copper saucepans, the heaps of maccaroni and pastes, of spices and drugs; the garlands of onions and red peppers and piles of apples; ... of the spectator, than the harmony of villainies, of various combinations of black and hog-like bestiality, and fox and wolf-like cunning and ferocity with wicked human thought and self-command, ... also, by the hand of two of her greatest poets, has given us a picture And first, of Roland Of him, of Count Orlando, we are told by Messer Lodovico Ariosto, that in consequence of his having...

Ngày tải lên: 21/02/2014, 21:20

... the content of the sentence compared to a human gold-standard Yet, the assessments of the two aspects were often the same—readability/fluency of the sentence is important for understanding the ... presence of uncommon difficult words are known to pose problems to readers and to render text less readable (CollinsThompson and Callan, 2004; Schwarm and Ostendorf, 2005) But these discourse- and ... phrase (where embedded phrases of the same type were counted only once) and for each phrase regardless of embedding Length of NPs/PPs contained in a VP The average number of words that constitute an...

Ngày tải lên: 22/02/2014, 02:20

... blot analysis of the hemolymph and ovary total protein of dsRNA-injected females (A) Hemolymph sample of NaCl ⁄ Piinjected and dsRNA-injected females Lanes 1–3: NaCl ⁄ Pi-injected and 4–6 dsRNA-injected ... monodon As there is a high degree of sequence similarity and structural conservation of these genes and gene products, confirmation of the true identity and function of these neuropeptides remains ... injection (Figs 4A and 5A) In conclusion, the use of recombinant protein or RNAi alone may not be sufficient to confirm the function of a neuropeptide The combined use of recombinant protein and RNAi described...

Ngày tải lên: 16/03/2014, 05:20

... structure of the last 32 amino acids sufficiently to be visible in electron density maps Substrate specificity of dNK Earlier crystallographic studies of substrates dT and dC and on the structure of the ... consisted of lL of crystallization solution containing 0.1 m Tris, pH 7.5, 0.2 m lithium citrate and 19% poly(ethylene glycol) 3350 added to lL of enzyme solution containing 10 mgÆmL)1 of protein and ... consisted of lL of crystallization solution containing 0.1 m MES, pH 6.5, 0.2 m lithium citrate and 18% poly(ethylene glycol) 3350 added to lL of enzyme solution containing 10 mgÆmL)1 of protein and...

Ngày tải lên: 16/03/2014, 06:20

... region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(b1– 3)GalNAc(b1-]n, which may be O-sulfated on the C4 and ⁄ or C6 of GalNAc and C2 of ... AC and ABC lyases generate disaccharides and tetrasaccharides [4] and have been widely used for the analysis of CS ⁄ DS composition These studies have yielded crucial data allowing an understanding ... allowing an understanding of species, tissue, age and pathology related differences and the estimation of changes in CS ⁄ DS abundance and composition However, the reduction of the polymer to its...

Ngày tải lên: 16/03/2014, 14:20

... In IB-367 and BM-1, the presence of strong daN(i,i + 1) and weak (or absence of) dMN(i,i + 1) and dbN(i,i + 1) NOE connectivities in the Gly3–Arg8 and Cys14– Gly16 regions of IB-367 and in the ... Arg1 and Arg17, and in the turn in the presence of Arg8 and Arg10 (see Figs and 4) Our conformational studies showed that BM-1 formed a twisted b-sheet structure, similar to that of native PG-1 and ... activity of the peptides A better understanding of the mode of action of these peptides is crucial for the development of a new generation of antibiotics It is known that, in the absence of both...

Ngày tải lên: 22/03/2014, 21:20

... series studies of air pollution and mortality This class of models includes a variety of predictors such as running means of temperature, non-linear functions of running means, multiple lags of temperature ... Zanobetti, A and Bateson, T (2003) Morbidity and mortality among elderly residents of cities with daily PM measurements In Revised Analyses of Time-series Studies of Air Pollution and Health, ... Peng and colleagues have explored this question, and the degree of bias that we might expect, using simulation analyses, and then applied these lessons to a large multicity database of PM10 and...

Ngày tải lên: 29/03/2014, 18:20

... glucose and two aldehydes (MG and GA) in inducing lipid and protein oxidation and antioxidant depletion of LDL particles as well as glycation of the apoB protein by measuring specific parameters of ... accumulation of AGEs, a number of studies have examined the role of low molecular mass aldehydes such as glyoxal, MG, and GA These materials are formed both as a consequence of oxidative processes and ... oxidation of low-density lipoprotein and whole serum Free Radic Res 36, 521–529 Thornalley, P.J (1996) Pharmacology of methylglyoxal: formation, modification of proteins and nucleic acids, and enzymatic...

Ngày tải lên: 31/03/2014, 01:20

... the design of the study and performed the electrical studies SK and MA carried out the structural studies SAA and MSA performed the thermal studies MAMK and MA also involved in writing of the manuscript ... Page of Figure DTA-TGA themogram of silver nanoparticles and above 300°C It can be generally attributed to the evaporation of water and organic components Overall, TGA results show a loss of 14.58% ... observed The copper and the carbon peaks correspond to the carbon coated copper grid of TEM No peaks of other impurity have been detected Therefore, the EDX profile of sample (inset of Figure 2) indicates...

Ngày tải lên: 21/06/2014, 02:20

... exemplary missions, SOFIA as the successor of the airborne Kuiper Observatory of the 1980s and early 1990s, JWST as the successor of Hubble, and the space probes Herschel and GAIA SOFIA and Herschel ... 7.3 × 109 M/M yr L/L (2.2) 2.2 The Quest to Understand the Formation of Stars 21 where M and and L are the mass and luminosity of the Sun and M and L the same for the star [858] Clearly, this development ... From Dust to Stars Studies of the Formation and Early Evolution of Stars Norbert S Schulz From Dust to Stars Studies of the Formation and Early Evolution of Stars Published in association...

Ngày tải lên: 03/07/2014, 21:09

... risk of approximately 1.4 [10] A series of “cradle to grave” cohort studies conducted in a succession of birth cohorts could unambiguously determine the origin of the observed LTP pattern Such studies ... feasibility of “real world” studies provides a justification for the use of simulation techniques to examine these issues The problem was first addressed using simulation in the 1990s by Giuffra and ... explore the possible impact of mortality in their simulations A more recent simulation study based on 12-month prevalence data from Australia and the Netherlands found evidence of recall bias because...

Ngày tải lên: 11/08/2014, 16:22

... HIV-2SBL and HIV-2ROD Env-mediated fusion, on the other hand, showed no appreciable lag time and 50% of maximum fusion was reached in 23 ± and 28 ± minutes, respectively Binding of HIV-1 and HIV-2 ... its CD4 and CXCR4 binding profile than HIV-2ROD gp120 (Figure 2) Specificity of binding was demonstrated by sCD4 inhibition of CD4 binding and a lack of CXCR4 binding in the absence of sCD4 (data ... in the design of the study and helped to draft the manuscript and RB conceived of the study, participated in its design and coordination and wrote the manuscript All authors read and approved...

Ngày tải lên: 13/08/2014, 09:20

... year-season, stage and number of lactation and cows; that of the Brown Alpine herd, year-season, stage and number of lactation, sire of the cow and genotypes at loci (in the case of the a,,-Cn expression, ... Jr Protein, lactose, and fat Sca 58, 817-827 (1975) Composition of the milks of dairy cattle protein fraction J Dairy contents and distribution of Golikova A.P & Panin I.A (1972) Ratios of protein ... expression of the B allele in a heterozygotes could, to -Cn sl -caseins so s1 degree, be a product of a of C-a protein co-migrating with the sl B-a protein For the B protein, the contribution of the...

Ngày tải lên: 14/08/2014, 20:20

... computing power and growing available protein structures from crystallographic and NMR studies, SMD simulation has become a powerful tool for exploring the dynamics of protein molecules SMD simulations ... mechanical stress and has also been observed in interactions involve some motor proteins (myosin and kinetochores) and some adhesive proteins (selectins and integrins) In contrast, the strand-swap dimer ... unbinding of Type I and Type II cadherins in SMD simulations 63 3.2 Dimer unbinding of Type I and Type II cadherins in AFM experiments 66 3.2.1 Control experiments 66 3.2.2 Type I and...

Ngày tải lên: 09/09/2015, 11:16

... SYNTHETIC STUDIES OF AZIDO-INOSITOLS AND INOSITOL-BASED GLYCANS SANDIP PASARI (M.Sc Indian Institute of Technology Madras, India) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... domains of protein- bound GPI anchor of P falciparum Figure 1.6 Molecular overview of glycan and lipid structural diversity of selected GPIs 10 Figure 1.7 Structural domains of PIMs ... cleavage of trans ketal and THP acetal of inositol derivatives 35 Scheme 1.23 Selective hydrolytic cleavage of inositol orthoformate 1-88 36 xiv Synthetic Studies of Azido-Inositols and...

Ngày tải lên: 09/09/2015, 17:55

... Appendix 1: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 1: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 1: NMR Spectra of the Selected Compounds in Chapter ... 2012 Appendix 1: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 1: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 1: NMR Spectra of the Selected Compounds in Chapter ... 2012 Appendix 1: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 1: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 1: NMR Spectra of the Selected Compounds in Chapter...

Ngày tải lên: 09/09/2015, 17:56

... Appendix 2: NMR Spectra of the selected Compounds in Chapter 2012 Appendix 2: NMR Spectra of the selected Compounds in Chapter 2012 Appendix 2: NMR Spectra of the selected Compounds in Chapter ... 2012 Appendix 2: NMR Spectra of the selected Compounds in Chapter 2012 Appendix 2: NMR Spectra of the selected Compounds in Chapter 2012 Appendix 2: NMR Spectra of the selected Compounds in Chapter ... 2012 Appendix 2: NMR Spectra of the selected Compounds in Chapter 2012 Appendix 2: NMR Spectra of the selected Compounds in Chapter 2012 Appendix 2: NMR Spectra of the selected Compounds in Chapter...

Ngày tải lên: 09/09/2015, 17:56

... Appendix 3: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 3: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 3: NMR Spectra of the Selected Compounds in Chapter ... 2012 Appendix 3: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 3: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 3: NMR Spectra of the Selected Compounds in Chapter ... 2012 Appendix 3: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 3: NMR Spectra of the Selected Compounds in Chapter 2012 Appendix 3: NMR Spectra of the Selected Compounds in Chapter...

Ngày tải lên: 09/09/2015, 17:56

... better understanding both multidimensional and global constructs of successful ageing and its measurements Study I and II would evaluate the validity of multidimensional and global models of successful ... two studies would shed light on how the two constructs of successful ageing could 17 be used and the advantages and disadvantages of using multidimensional and global models In studies III and ... Dimension of successful ageing model: Assessment 66 of spirituality and religiosity component  3.2.3.8 Dimension of successful ageing model: Assessment 67 of quality of life  3.2.3.9 Dimension of...

Ngày tải lên: 10/09/2015, 09:02