Design of Runner System
... the molded part If these requirements are met, the sprue in single-cavity molds is pulled from the orifice and thus demolded by the molded part, which remains on the ejector-side of the mold ... Systems Just as hot runners are used in molds for thermoplastics, cold runners are used in molds for reactive materials such as thermosets and rubber Unlike the hot mold, which is kept at 160-180 C, ... temperature Sensitivity to heat Shrinkage Molding machine Injection mold Type of clamping Injection pressure Injection rate Automatic demolding Manual demolding Temperature of runner system Functions...
Ngày tải lên: 13/10/2016, 22:32
... for two molds and two different gates, one can pick up the changes of processing conditions and obtain help in designing molds and, above all, gates The complete processing window for molds C ... index of 10%, mold D allows a maximum mold temperature of only 164 C This results in a significantly increased minimum processing time for mold D compared to C The latter allows a mold temperature ... of mold B The position of the injection limit remains about the same in both molds Figure 5.87 compares the effect of different cavity depths h c during the injection phase (mold A: h c = mm; mold...
Ngày tải lên: 13/10/2016, 22:33
... made up 61.69%-71.39% of the total saponins in the XST injection (total saponins nominal: 50 mg/ml) The ten saponins with available standard substances were quantitatively determined and made up ... high-performance liquid chromatography coupled with diode array detector and mass spectrometry J Chromatogr A 2009, 1216:2071-2078 World Health Organization: Guidelines for the Assessment of Herbal ... rate of 20 arbitrary units; capillary temperature, 350°C; capillary voltage, -15 V; tube lens offset voltage, -30 V Full scan data acquisition was performed from m/z 80 to 1800 in MS scan mode...
Ngày tải lên: 13/08/2014, 14:20
Báo cáo " Pattern and determinant agents of the debris and mud flash flood in Lay Nua Commune area, the Former Muong Lay District, Dien Bien Province " docx
... the Lay Nua Commune, where its width has doubled (1200 m, Fig. 1). From Lay Nua upstream the river adopts the direction of N‐E 400 and its width reduces quickly. ... (T2-3 lc) at the bed. According to geophysical data, the described faults plunge abruptly eastwards with a dip of 850 and the destruction zone of 1‐2km in width [3]. ... forming we have concentrated on investigating the two most important aspects, namely source of detritus material supply for flash flood and slope stability propitious for triggering the landslide and ...
Ngày tải lên: 22/03/2014, 12:20
báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps
... http://www.magneticmicrosphere.com/ suppliers/magnetic_microspheres.php Meanwhile, designing a purification procedure employing magnetisable solid phase support has become one of the interesting ... overexpression systems [4,5,15] Given their wide application in protein purification, setting the optimal conditions up to achieve the best recovery, yield and purity covering the wide range of recombinant ... exhibited superior capacity when % recovery for both proteins was concerned Three matrices also showed variable yields with similar pattern as recovery As a whole, SiMAG/N-NTA/Nickel bead was superior...
Ngày tải lên: 11/08/2014, 00:23
Báo cáo y học: "Qualitative and quantitative research into the development and feasibility of a video-tailored physical activity intervention" pptx
... monitoring recording equipment Prior to conducting the focus groups the researchers had developed a focus group discussion guide This guide was developed in a way that would promote open discussion, ... within the Focus Groups Outcomes section reflect the topics discussed during the focus groups) All the focus groups were audio-recorded Following completion of five focus groups (FG1 to FG5) the ... applied focus groups (qualitative) and a state-wide survey (quantitative) to inform the development of a video-tailored physical activity intervention Focus groups Focus groups were conducted...
Ngày tải lên: 14/08/2014, 08:20
Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... fluoresces upon interaction with nucleic acids and penetrates only cells with leaky plasma membranes [40] As can be seen in Fig 5(A), SYTOX green uptake in N crassa rose significantly upon treatment ... lgÆmL)1) was added and superoxide production was measured for 10–15 by a Biolumat 9505 Peak luminescence values were compared and inhibition was calculated as a percentage of superoxide production ... absorbance at 595 nm was recorded after 20 h of incubation SYTOX green uptake Fungal membrane permeabilization was measured by SYTOX green uptake as described previously [35] Absolute values of fluorescence...
Ngày tải lên: 21/02/2014, 15:20
NATIONAL REPORT OF MALAYSIA ON THE FORMULATION OF A TRANSBOUNDARY DIAGNOSTIC ANALYSIS AND PRELIMINARY FRAMEWORK OF A STRATEGIC ACTION PROGRAMME FOR THE BAY OF BENGAL potx
... 33 2.2 : Composition of Marine Fish Species Group Landings, West Coast Peninsular Malaysia (2000, 1980, 1990) 41 2.3 : Contribution by Gear Group to Total Landing, West Coast Peninsular Malaysia ... Putrajaya, Malaysia Dr K Kuperan Viswanathan ICLARM The World Fish Centre Penang, Malaysia Prof Dr Mohd Ibrahim Hj Mohamed Professor Faculty Science and Environmental Studies UPM, Serdang, Malaysia ... Asia and occupies a total land area of 330,434 square kilometres The land mass comprises three main components: Peninsular Malaysia and the two states of Sabah and Sarawak, which occupy the coastal...
Ngày tải lên: 06/03/2014, 15:21
Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... lac1 transcription were studied by growing the fungus on basal medium without FA for days before supplementing cultures with different concentrations of FA as indicated Total RNA was extracted from ... effect of different aromatic compounds on laccase production was determined after 36 h following supplementation of day-old cultures with mM (final concentration) of the test compound For purification ... relative humidity The bags were removed after 14 days to promote fruiting Samples were taken from duplicate cultures at different stages of the mushroom developmental cycle: early, middle and late...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... and probe sets Based on human genome sequencing, ABI has identified and constructed several primer probe sets at multiple intron–exon junctions to quantitate the variants We used three sets of primers ... and VBARP-S exhibited a distinct cytoplasmic pattern upon expression Similar cytoplasmic distribution was observed in 293 and A172 cells upon transfection with VBARP constructs (data not shown) ... (lanes 7–9) antibodies and resolved on SDS ⁄ PAGE Arrows indicate the respective protein size (Inset) In vitro translated products of VBARP isoforms, Vpr and Nef used in coimmunoprecipitation assay...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
... the h angle The methyl group of b2-HAla occupies a position close to that of CH2b of Pro or the pro S methyl in Aib or Ala Conversely, the methyl group of b3-HAla occupies a position similar to ... compound [36], it is likely that the additional methylene group of b2-HPhe causes steric hindrance within the receptor as does the methyl group in Ca-MePhe Although position of SP can accept larger ... associated with the NK-1 receptor in CHO transfected cells [24,25] have been classified in two groups In one group, peptides are as potent as SP, or even more potent, whatever the binding site considered,...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Functional fine-mapping and molecular modeling of a conserved loop epitope of the measles virus hemagglutinin protein pdf
... mimic the hydrophobicity of the thiol group by a methyl group Monoclonal antibodies and ELISA Monoclonal antibodies [8] were harvested from hybridoma supernatant produced in a Cell-Line system ... Preparation of sensor surfaces Reporter HNE peptide was coupled to the sensor surface as described by the supplier (BIAapplications Handbook, Biacore, Uppsala, Sweden) Briefly, a 100 lM solution of oxidized ... activation of free sulfhydryl groups or by N-hydroxy-succinimide/ N-ethyl-N¢-[(3-dimethyl-amino)propyl] carbodiimide hydrochloride coupling of an e-amino group of an additional N-terminal lysine...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... program of the PHYLIP package version 3.57c [15] Support for the inferred clades was obtained by bootstrap analysis from 1000 replications of the data set using the SEQBOOT and CONSENSE programs Phylogenetic ... an upright arrow (›) (B) The exon/intron boundaries of the split codons for arginine (between exon and exon 5) and valine (between exons and exon 7) are shown Exonic regions are shown in uppercase ... putative polyadenylation (ATTAAA) signals: one is located 18 bp upstream from the poly(A) of GT-cDNA1 and another is located 11 bp upstream from the poly(A) of GT-cDNA2 (data not shown) Of particular...
Ngày tải lên: 17/03/2014, 03:20
Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
... centrifuged at 10 000 g for 10 The supernatant was mixed with vol cold ()20 °C) acetone by gentle stirring and then kept in a freezer ()35 °C) for 15 The supernatant was decanted after centrifugation ... some bacterial cellulases, those belonging to GHF5 subgroup The overall identities and similarities of P hilaris cellulase to GHF subgroup members were: 49% and 67% to Pseudomonas fluorescence ... 6.8) The suspension was centrifuged at 20 000 g for to remove insoluble materials, and then the supernatant was loaded on a gel for native PAGE and activity Ó FEBS 2003 staining After electrophoresis,...
Ngày tải lên: 17/03/2014, 10:20
Retrieval of the source location and mechanical descriptors of a hysteretically-damped solid occupying a half space by full wave inversion of the the response signal on its boundary doc
... parameters of the set P are strictly equal to their counterparts in the set p and the values of all the parameters of the set N are strictly equal to their counterparts in the set n, the response ... except PK of the set P are strictly equal to their counterparts in the set p and the values of all the parameters of the set N are strictly equal to their counterparts in the set n, then the inversion ... appeals The physical/geometrical parameters of the RM form the set P, whereas the numerical parameters of the RM can be grouped into a set which we call N When, as in the present study, the (true)...
Ngày tải lên: 18/03/2014, 01:21
Báo cáo khoa học: N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell on the virion surface docx
... represented by superposition of three bands corresponding to absorption of OH bonds in tightly bound OH groups (the band at 3240 cm)1), in weakly bound OH groups ( 3440 cm)1) and in free OH groups of ... spectra of peak 2* material before and after CPY treatment are shown (upper and lower parts, respectively) Mass values in the upper part correspond to ions of sodium and potassium salts of the glycosylated ... deformational amide group vibrations (amide II, 1550 cm)1, and amide III, 1300 cm)1) can be seen (Fig 6) A broad-band in the 3100–3700 cm)1 region corresponds to superposition of several...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... b-T1 These results support the hypothesis that the CCT-mediated folding evolved early in the eukaryotic lineage 150) Each suspension was centrifuged at 14 100 g and the supernatant used as cytoplasmic ... rabbit reticulocyte lysate The upper and lower open arrowheads in lane indicate the location of b-T1/CCT complex, and the b-T1/Cof A complex, respectively The upper, middle and lower solid arrowheads ... polypeptide P259–T372 with the same region of the Euplotes consensus sequence, obtained from the alignment of the known b-tubulin sequences for Euplotes species (accession numbers P20365 and Q08115,...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo khoa học: Identification and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus influenzae pptx
... of selected precursor ions with nitrogen in the RF-only quadrupole collision cell, were mass analysed by scanning the third quadrupole NMR experiments were performed on Varian INOVA 600 and 500 ... spectrum of the O-deacylated LPS obtained from strain RM118 lpsA grown on BHI plates supplemented with Neu5Ac Inset, complete 1H-NMR spectrum The spectrum was recorded in D2O at pH 7.0 and 295 K ... prepared for structural analysis from cells harvested after growth on batches of 20 BHI plates, supplemented with Neu5Ac LPS was extracted by the hot phenol/water method [24] and O-deacylated as...
Ngày tải lên: 23/03/2014, 21:21