modulation of hormone receptor signalling

... the functional activities of GRIP1 They extend our understanding of the importance of the structural status of GRIP1 in modulating NR functions Results Autoregulation of GRIP1 AD activities by ... conserved AF-2 domain of NRs [22] The C-terminal region of the p160 co-activator can interact with the AF-1 domain of some NRs and enhance their AF-1 activities in the absence of ligands [23–25] ... 10 WB anti-HuR Fig Modulation of glucocorticoid receptor- interacting protein (GRIP1) transactivation activity (A, B) Expression vectors (0.5 lg) for the indicated fragments of GRIP1 fused to the...

Ngày tải lên: 07/03/2014, 12:20

... Identification of the critical sequence elements in the cytoplasmic domain of leptin receptor isoforms required for Janus kinase ⁄ signal transducer and activator of transcription activation by receptor ... LEPRb is capable of activating a broader range of STAT factors than other cytokines such as interleukin-6 (IL-6) and growth hormone (GH) Analysis of point mutants revealed that each of the individual ... replaced either one of the three tyrosines or combinations of them were expressed in HIT-T15 cells The specific signalling capacities of each tyrosine residue in the intracellular domain of LEPRb can...

Ngày tải lên: 07/03/2014, 16:20

... [6] P2Y receptors, like P1 receptors, are members of the superfamily of G protein-coupled receptors (GPCR) and P2X receptors are ligand-gated ion channel receptors The overall structure of the ... is also shared with certain signalling pathways coupled to GPCRs Many members of this family of receptors (b-adrenergic receptors, muscarinic receptors, endothelin receptors, rhodopsin) are located ... this subfamily of receptors in cellular signalling make them attractive therapeutic targets The coupling between stimulation of the P2X receptor and the generation of intracellular signalling cascades...

Ngày tải lên: 30/03/2014, 02:20

... Pituitary adenomas of patients with acromegaly express thyrotropin-releasing hormone receptor messenger RNA: cloning and functional expression of the human thyrotropin-releasing hormone receptor gene ... subtypes of brain Xenopus thyrotropin-releasing hormone receptor were obtained and designated xTRHR1, xTRHR2 and xTRHR3 Amplification of cDNA ends The information on the nucleotide sequence of the ... desensitization of the responses (Lower) Responses of the three xTRHR subtypes to different concentrations of TRH The maximum current amplitude of each recording was measured and reported as a function of...

Ngày tải lên: 21/02/2014, 03:20

... a cofactor of Rev-erbb A B C Fig Colocalization of ZNHIT-1 and Rev-erbb in Hela cells (A) Nuclear localization of GFP-Rev-erbb (B) Colocalization of RFPZNHIT-1 and GFP-Rev-erbb (C) Effect of ... V & Danan JL (2000) Modulation of the far upstream enhancer of the rat a-fetoprotein gene by members of the RORa, Reverba and Rev-erbb groups of monomeric orphan nuclear receptors DNA Cell Biol ... atherosclerosis [21,22] Therefore, a better understanding of the regulation of the expression of the apoCIII gene is of major importance for the treatment of dyslipidaemia In this study, a zinc finger HIT...

Ngày tải lên: 07/03/2014, 05:20

... which binding of the hormone to the N-terminal domain of the receptor translates into activation of its serpentine domain is unknown With the aim of improving our understanding of the mechanisms ... form of the receptor; the active conformation capable of promoting GDP/GTP exchange on the G-protein is achieved by a fraction of the unliganded receptors only, but is stabilized by binding of ... when combined with an activated form of the receptor Materials and methods Construction of mutant TSH receptors The constructs harboring the single mutated TSH receptors T477I, I486M and P639S have...

Ngày tải lên: 08/03/2014, 08:20

... characterization of a novel trans-factor for luteinizing hormone receptor mRNA from ovary J Biol Chem 279, 14937–14944 Nair AK & Menon KM (2005) Regulation of luteinizing hormone receptor expression: ... of LHR mRNA to MVK The inhibitory effect of mevalonate was found to be less pronounced when compared with that of ATP There was a significant decrease at a concentration of ATP or mevalonate of ... studies have shown that binding of MVK to LHR mRNA suppresses the translation of mRNA in vitro [14] As the mutation of any of the four amino acids at the active site of MVK decreases its binding...

Ngày tải lên: 16/03/2014, 06:20

... Rh1 where R represents the concentration of hormone- free receptor molecules, h is the hormone concentration, Rh is the concentration of the hormone- loaded receptor molecule, and Kd is the dissociation ... carbaryl Interaction of a receptor with a single ligand The simplest model to describe dose response curves assumes an equilibrium between hormone- free and hormone- loaded hormone receptor molecules ... agonist molecule binding to each receptor molecule, and an hormone concentration much larger than the receptor concentration The fraction of receptor bound to the hormone Fr can be described by...

Ngày tải lên: 16/03/2014, 10:20

... caveolae [27] Insulin receptor signalling in caveolae We next examined the functional status of caveolaelocalized receptors After incubating cells with a submaximal concentration of insulin the insulin-induced ... 6C) of the insulin receptor was not affected by this level of prior cholesterol depletion, nor was the total tyrosine phosphorylation of IRS1 affected (Fig 6D) A more detailed examination of the ... properties of insulin receptor signalling in rat and human tissues This again stresses the importance of examining human cells and tissues b-Cyclodextrin is widely used to control cellular levels of...

Ngày tải lên: 23/03/2014, 12:20

... levels of the expression of each cDNA and of the reliability (Present/Absent call) were calculated with software (AFFYMETRIX MICROARRAY SUITE ver.4.0) For comparisons of the expression level of each ... treatment of the HeLaTR cells with T3, overexpression of 4-1BB in the HeLaTR cells led to the activation of caspases Activation of caspases by the overexpression of 4-1BB was not an artefact of overexpressing ... involvement of 4-1BB-L in the activation of caspases by T3 The biological significance of the modulation of PSG7, fmfc and BMP-6 expression by T3 also awaits additional studies Up-regulation of 4-1BB...

Ngày tải lên: 23/03/2014, 18:20

... activity of the GnRHR promoter was determined by comparing the luciferase activity of the promoter in the presence of pFoxl2 (over-expression) to the activity of the promoter in the absence of Foxl2 ... Mutations in Page of 12 the soluble form of the EDA protein and the EDA receptor are the cause of anhidrotic ectodermal dysplasia, a syndrome that results from impaired development of skin appendages ... localization of long form prolactin receptor messenger RNA in lactating rats by biotin-labeled riboprobe Endocr J 1995, 42:69-76 47 Navickis RJ, Jones PB, Hsueh AJ: Modulation of prolactin receptors...

Ngày tải lên: 20/06/2014, 07:20

... soluble receptors, but the identification of oral, small molecule inhibitors of IL-13 would have obvious advantages We therefore sought to examine whether the previously reported modulation of IL-13, ... proliferation This effect on Page 10 of 12 Th2 cytokines, via a modulation of Th2 activation, may result from a role of H4R in the Th2 priming capability of dendritic cells [6] The exact mechanism ... treatment and in support of a direct relationship of these remodeling parameters to IL-13 levels, the extent of their amelioration by JNJ 7777120 was proportional to its reduction of IL-13 levels in...

Ngày tải lên: 12/08/2014, 11:22

... and altered receptor usage Receptor usage of Envs The GL8 strain of FIV requires determinants in both CRDs and of its primary receptor CD134 for infection; chimaeric receptors based on human CD134 ... evolution of the virus -receptor interaction may not simply be a modulation of binding affinity, rather it could represent a shift in the way the Env and receptor interact The two modes of interaction ... neutralising activity of the PM plasma from A613 targeting V5 Following exchange of the V4-V5 region of ABSN with that of the B14, B19 and B28, we assessed the sensitivity of the chimaeric Env-bearing...

Ngày tải lên: 12/08/2014, 23:23

... induction of SOCS and both the presence of sepsis and the administration of PN [14] Administration of 16 hours of PN was associated with induction of the expression of hepatic mRNA of the SOCS ... serum concentrations of a pleiotropic cytokine (IL-6) and an anti-inflammatory (IL-10) cytokine and the expression of mRNA of SOCS proteins, of IGF-I and of the growth hormone receptor (GHR) in ... sepsis independent of nutrition • Provision of nutrition increased mRNA of the GHR and of IGF-I • These findings suggest that provision of nutrition and the route of delivery of nutrition in sepsis...

Ngày tải lên: 13/08/2014, 08:20

... Fujii-Kuriyama Y, Kato S: Modulation of oestrogen receptor signalling by association with the activated dioxin receptor Nature 2003, 423:545-550 Shipley JM, Waxman DJ: Aryl hydrocarbon receptor- independent ... effect showed a parallel pattern of expression with ERα gene expression [20] Modulation of AhR responsive genes We investigated the effects of NP on PCB-77-induced AhR signalling It should be noted ... (% of control) Control 2500 c 40 1.0 800 200 ab 60 [PCB-77, μM] 1000 b 80 Control AhRR (% of control) a 100 300 CYP1A1 (% of control) PCB-77 alone + μM NP (B) AhR a AhR (% of control) AhR (% of...

Ngày tải lên: 13/08/2014, 13:20

... dendritic cells One class of receptors present on the surface of these cells, which are capable of dictating a cells function, are Fcγ receptors and modulation of Fcγ receptors has been shown ... surface of osteoclast progenitors but is unable to inhibit bone loss in the model of osteoporosis In addition to studying the role of Fcγ receptor modulation of osteoclastogenesis, the role of Bcl-3 ... Fcγ receptor mediated modulation of osteoclastogenesis James Joseph Doonan A thesis submitted to the College of Medicine, Veterinary and Life Sciences, University of Glasgow in fulfilment of the...

Ngày tải lên: 22/12/2014, 20:59

... Validation of E1 in g/L of PBS/BSA Table 9b Intraday Validation of E1 in g/L of PBS/BSA Table 10a Interday Validation of E2 in g/L of PBS/BSA Table 10b Intraday of Validation of E2 in g/L of PBS/BSA ... Validation of A4 in g/L of PBS/BSA Table 5a Interday Validation of T in g/L of PBS/BSA Table 5b Intraday Validation of T in g/L of PBS/BSA Table 6a Interday Validation of DHT in g/L of PBS/BSA Table ... Preparation of Different Doses of Drugs 116 4.2.3.2 Culture of CWR22Rv1 for Dose Dependent Curve 116-117 4.2.3.3 Culture of CWR22Rv1 under Saturating Doses of Drugs 4.2.3.4 Preparation and Extraction of...

Ngày tải lên: 09/09/2015, 08:14

... diversity of pathways downstream of TLR3 1.2.5 Critical signalling components of TLR pathway Adaptor proteins play a critical role immediately downstream of TLR signalling by coupling the receptor ... an arsenal of receptors known as pattern recognition receptors (PRR) that can recognise PAMPs and evoke an immune response These receptors include toll-like receptors (TLR), NOD-like receptors ... 1.3 Positive and negative regulators of TLR signalling I-15 Other Pathogen Recognition Receptors I-17 1.3.1 RIG-I-like Receptors I-17 1.3.2 NOD-like Receptors I-21 1.4 Transcription factor...

Ngày tải lên: 09/09/2015, 10:16

... during my last two years of study i TABLE OF CONTENTS ACKNOWLEDGEMENTS i TABLE OF CONTENTS ii SUMMARY vi LIST OF TABLES viii LIST OF FIGURES ... N-methyl-D-aspartate receptor (NMDAR): a key player in learning and memory 15 1.3.1 Characteristics and expression of NMDAR 16 1.3.2 Functions of NMDAR 19 1.3.3 Modulation of NMDARs ... Objectives of study 61 3.3 Results 63 3.3.1 Expression of human apoE in brains of huApoE-knockin (apoEKI) mice across three time points 65 3.3.2 Expression of apoE receptor...

Ngày tải lên: 30/09/2015, 06:42

... all their support during the ‘darkest days’ of my life i TABLE OF CONTENTS Page List of Figures iv List of Tables v List of Abbreviations vi Summary of Thesis viii Chapter 1: Introduction Chapter ... in the limbic structures is a result of hyperexcitability of the epileptic brain and is thought to be mediated by activation of glutamate receptors The aim of this study was to focus on the role ... glutamate receptor (mGluR) and the effect of selective blockage of mGluR1α as a post- treatment of SISE In this current work, an initial effort was concentrated on the establishment of an in vivo...

Ngày tải lên: 26/11/2015, 10:50