modulation of caspase activity by cellular inhibitors

... Expression of either STE20 or CLA4 from multicopy plasmids did not alter the levels of SE (Fig 6B) A higher concentration of SE in cla4D cells could be explained by a negative regulation of Are2p activity ... solution consisting of 0.63 g of MnCl2Æ4H2O, 60 mL of water, 60 mL of methanol and mL of concentrated sulfuric acid, briefly dried and heated at 100 °C for 20 SE were then quantified by densitometric ... for 30 at 30 °C and terminated by the addition of 300 lL of chloroform ⁄ methanol (2 : 1, v ⁄ v) Lipids were extracted twice for 10 with shaking using 300 lL of chloroform ⁄ methanol (2 : 1; v ⁄...

Ngày tải lên: 18/02/2014, 13:20

... of the mechanism for inactivation of the enzyme may come from measurement of the effect of the concentration of the aromatic cofactor responsible for the onset of oscillations Here we use the fact ... addition, the rate of inactivation is slowed down by increasing the concentration ể FEBS 2003 Protection of peroxidase activity (Eur J Biochem 270) 2799 Fig Eect of pH on the rate of enzyme decay ... increasing the concentration of the aromatic cofactor Note that the steady state concentration of superoxide is essentially independent of the concentration of the cofactor The difference in steady...

Ngày tải lên: 20/02/2014, 11:20

... plasma cofactor required for plactin activity (A) Structures of plactin D and its analogs DNS, dansyl (B) The activities of plactin D, plactin-14 and plactin-14–DNS to enhance cellular u-PA activity ... proteolytic cleavage of scu-PA When prothrombin was used in place of plasma to determine plactin cofactor activity, it did not support plactin D-dependent enhancement of u-PA activity in U937 cells ... D Xa // PL Xa PL 250 Xa activity 60 C Xa // PL // Ca2+ // Va Xa PL Ca2+ Va 1200 Xa activity Xa activity 80 Generation of thrombin activity (Δ A405·h–2 × 103) B Xa Xa Xa activity A 100 10 20 30...

Ngày tải lên: 07/03/2014, 00:20

... Down-regulation of telomerase activity by induction of differentiation of HL-60 cells has been reported [24,25] To understand the role of each telomerase subunit in enzyme activity, we used downregulation of ... experiment, because of the shortage of cellular salvage pathways and the complete inhibition of TEP1 function by high concentrations of antisense oligonucleotides, telomerase activity was dramatically ... reconstituted complex of hTERT and hTR was detected by EMSA, and its activity was stimulated more than 30-fold by the addition of cell extract, indicating the presence of a cellular factor contributing...

Ngày tải lên: 17/03/2014, 23:20

... mechanism of SSIII activity modulation by SBD cannot be deduced until the enzyme conformation is elucidated, the data presented here contribute to a better understanding of how SBDs modulate enzyme activity, ... (including several SS isoforms) are capable of associating in a multisubunit complex, and that these interactions may be of physiological importance [13,14] One of the soluble SS isoforms, SSIII, has ... N-terminal region of SSIII can interact with SSI [13] The possible regulatory role of this protein makes this isoform a potential target for the manipulation of the level and quality of plant starch...

Ngày tải lên: 22/03/2014, 21:20

... constant of the enzymeinhibitor complex in the absence of substrates, but estimates the apparent Ki in the absence of H2O2 and in the presence of 10 mm ABTS The effect of Ni2+ on the oxidation of ABTS ... produced by addition of an equimolar amount of H2O2 Compound II with characteristic absorption maxima at 278, 413, 541 and 576 nm was generated from compound I by the addition of one equivalent of ... peroxidase in the presence of 30 mM Ni2+ before and after addition of an equimolar amount of H2O2 Arrows indicate the disappearance of compound I after addition of one equivalent of ascorbic acid Buffer...

Ngày tải lên: 30/03/2014, 04:20

... accompanied by a persistent state of oxidative siege, and the survival of a given cell is determined by its balance of reactive oxygen intermediates and antioxidants Disturbance of this balance ... amounts of SPPV induced enhancement of IL-12 due to ability of IL12 to induce early phase of NK and T cell activation [34] Using CRBC as model of TD Ag, it has been shown that SPPV was capable of ... instructions SOD activity was also assessed in peritoneal macrophages' culture supernatants by means of the inhibition of pyrogallol autoxidation as described [22] One unit of Page of (page number...

Ngày tải lên: 18/06/2014, 22:20

... manipulates OF parameters to enhance walking ability after stroke The specific aims of this study were: first, to compare the non-volitional modulation of walking speed in response to an OF of variable ... http://www.jneuroengrehab.com/content/4/1/22 modulation of walking speed in response to OFs of changing speed, would still present sufficient modulation such that a paradigm based on OF speed manipulation could ... carried out in Statistica 7.0 ® and the level of significance was set p < 0.05 Results Experiment Examples of modulation of walking speed in response to an OF of continually changing speed are illustrated...

Ngày tải lên: 19/06/2014, 10:20

... accompanied by a persistent state of oxidative siege, and the survival of a given cell is determined by its balance of reactive oxygen intermediates and antioxidants Disturbance of this balance ... amounts of SPPV induced enhancement of IL-12 due to ability of IL12 to induce early phase of NK and T cell activation [34] Using CRBC as model of TD Ag, it has been shown that SPPV was capable of ... instructions SOD activity was also assessed in peritoneal macrophages' culture supernatants by means of the inhibition of pyrogallol autoxidation as described [22] One unit of Page of (page number...

Ngày tải lên: 20/06/2014, 04:20

... stated by Genty et al (1989), and confirmed by the remarkable stability of the Φ II /Φ erelationship under a wide range of conditions (review by Edwards and Baker, 1993) The computation of ... al, 1992) The contributions of stomata (+ boundary layer) and of mesophyll transport to the overall limitation of CO influx have been shown to be of the same order of magnitude in many cases (Lloyd ... all of them are heterobaric We therefore used combined measurements of gas exchange and chlorophyll fluorescence to estimate the availability of CO in the chloroplasts of different species of...

Ngày tải lên: 08/08/2014, 18:21

... dominance of extracellular collagen matrix over cellular structures, together with disruption of the tissues at pretreatment, did not allow for quantitative analysis Characterization of periarticular ... regulated at a number of different levels, and there is evidence that secondary modulation of signalling pathways downstream of Fas may alter significantly the susceptibility of cells to Fas induced ... al.: Development of criteria for the classification and reporting of osteoarthritis Classification of osteoarthritis of the knee Diagnostic and Therapeutic Criteria Committee of the American Rheumatism...

Ngày tải lên: 09/08/2014, 01:23

... reduction of Sox9 activity by atRA is independent of NF-κB activation Binding of protein complexes to the Col2a1 48-bp minimal enhancer and nuclear Sox9 levels Since Sox9 activity was decreased by TNF-α ... were analyzed by densitometry using Kodak Digital Science software (Eastman Kodak, Rochester, NY, USA) Data were analyzed by paired t-tests, or by analysis of variance followed by Tukey's multiple ... activity [21] Upon administration of retinoic acid, NF-κB activity is reduced, which suggests an effect of RARs on function rather than expression of NF-κB Recombinant NF-κB p65 and members of...

Ngày tải lên: 09/08/2014, 10:22

... synthesis of melanin by hydroxylation of a monophenol and the subsequent oxidation of o-diphenols into o-quinones The biosynthesis of this rate-limiting enzyme in melanogenesis is modulated by cell-signaling ... the phosphorylation of p38 (Figure 4B, at 10 min) leading to the decline of p-CREB Cellular localization of Cpc was investigated by immunoblot analysis and confocal immunofluorescence localization ... effect Figure 2A shows the changes of cellular tyrosinase activity and melanin content with the stimulation of a-MSH (20 nM) It was observed that the tyrosinase activity and melanin formation were...

Ngày tải lên: 10/08/2014, 10:20

... expression plasmid of HDV ribozyme Methods The bel7402, HCT116 cells were given by Department of molecular Biology, Shandong University, DNA of HDV ribozyme was synthesized by Shanghai Biosun ... to meet the need of cleavage of telomerase We called the new Figure Structure of antigenomic ribozyme of HDV (g.RZ 1/84) Telomerase RNA plasmid construction We cloned a portion of hTR component ... of control RZ, no obvious catalytic activity was detected One cleavage process was shown at molar ratio 5:1 and at the temperature 45°C in Figure The telomerase activity Cellular telomerase activity...

Ngày tải lên: 10/08/2014, 10:20

... inflammation in a mouse model of allergic asthma The effects of VD on airway inflammation may result from its inactivation of ERK1/2 and downregulation of NPRA expression Analysis of ERK1/2 expression ... day 28 (mice were wks of age)   E D C B A @ RT-PCR detection of NPRA expression in the lung For analysis of the effect of VD on NPRA expression in vivo, three groups of mice (n = 4) were intranasally ... done as control Expression of ERK1/2 and phosphorylation of ERK1/2 were analyzed by western blot There was no significant change in expression of the total amount of ERK1/2 (Fig 2A); however,...

Ngày tải lên: 12/08/2014, 14:20

... role of zinc in the molecular mechanism of Tat-induced apoptosis, we analyzed the conformations of the apo-form and zinc-bound form of Tat, and studied the interaction of the two forms of Tat ... the apoptosis of macrophages and cytotoxic T-lymphocytes by several mechanisms [9] These different pathways include the up-regulation of Fas ligand [10], the down-regulation of cellular genes ... Since apoptosis mediated by Tat partly relies on the interaction of Tat with tubulin [14-17], we hypothesized that zinc binding might play a role in the modulation by Tat of the microtubule dynamics...

Ngày tải lên: 13/08/2014, 05:21

... reduction of P-TEFb activity, thus the inhibition of Tat activity is unlikely due to a global reduction of cellular P-TEFb activity To further investigate the mechanism of inhibition of Tatmediated ... determinants of HEXIM1 protein in repression, the activity of Gal4-CycT1 on G5HIV- Luc was monitored in the presence of co-transfected Flagtagged deletion mutants of HEXIM1 We found that removal of the ... inhibition of Tat activity is unlikely due to a global inhibition of P-TEFb activity Moreover, we found that basal transcription from the LTR sequences was largely unaffected by over-expression of HEXIM1...

Ngày tải lên: 13/08/2014, 09:21

... 90 Curriculum Vitae x LIST OF TABLES Table Changes in apoptotic genes as a result of loss of CHD8 72 xi LIST OF FIGURES Figure Schematic of functional domains of serum response factor (SRF) ... process of cellular differentiation can be divided into three main components: (a) activation of specific genes that are required for differentiation of a cell, (b) control of expression of these ... in the phenotypic modulation of smooth muscle cells However, it is ultimately the balance between the expression of MMPs, the expression of their inhibitors (tissue inhibitor of metalloproteinase,...

Ngày tải lên: 24/08/2014, 11:38

... Inactivation of EAAT2 has been reported to be facilitated by several possible mechanisms; such as the cleavage of EAAT2 by caspase- 3, oxidation of EAAT2 by mutant SOD1 and/or inactivation of EAAT2 by splicing ... 34 1.3.2 Neurofilament function…………… .36 1.3.2.1 Biological function of neurofilaments 36 1.3.2.2 Function of neurofilaments is regulated by phosphorylation… 36 1.3.3 Neurofilaments ... ALS 30 NEUROFILAMENTS……………… .33 1.3.1 Molecular Biology of Neurofilaments… 33 1.3.1.1 Structure and assembly of neurofilaments… .33 1.3.1.2 Transport of Neurofilaments…………………...

Ngày tải lên: 10/09/2015, 09:29

... MODULATION OF DRUG TRANSPORT BY CITRUS FRUIT JUICES LIM SIOK LAM (B.Sc (Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF PHARMACY NATIONAL UNIVERSITY OF ... (Papp) of R-123 across Caco-2 cell monolayers and (b) cellular accumulation of R-123 by basal membrane of Caco-2 cells exposed to: transport medium (TM), 100 µM of verapamil (V), and 10% and 50% of ... and anti-proliferative studies 93 3.3.3 Modulation of P-gp expression in L-MDR1 cells 97 3.3.4 Modulation of P-gp expression in vivo 99 3.3.5 Modulation of mdr1a mRNA in vivo 101 3.4 Discussion...

Ngày tải lên: 14/09/2015, 18:34