modulation of androgen receptor expression

... Journal of Biomedical Science 2011, 18:6 http://www.jbiomedsci.com/content/18/1/6 Page of 11 Figure Differentiation status and AR expression profile of PCSCs under the influence of Hedgehog overexpression ... active form of Gli2; Gli2-rep: repressed form of Gli2; Gli3-act: active form of Gli3 Chang et al Journal of Biomedical Science 2011, 18:6 http://www.jbiomedsci.com/content/18/1/6 Page of 11 Figure ... biology of the prostate: stem cell biology Urology 2003, 62:11-20 Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development of prostate...

Ngày tải lên: 10/08/2014, 05:21

... 0.676 AR = androgen receptor, ER= estrogen receptor, PR= progesterone receptor < 10% positive nuclei used as cutoff for AR, ER and PR positivity ± Mann Whitney u-test for comparison of medians ... immunohistochemical evaluation of androgen and progesterone receptors in ovarian tumors Hum Pathol 1993, 24:90-95 Lee P, Rosen DG, Zhu C, Silva EG, Liu J: Expression of progesterone receptor is a favorable ... Gynecol Oncol 2005, 96:671-677 Lau KM, Mok SC, Ho SM: Expression of human estrogen receptor- alpha and -beta, progesterone receptor, and androgen receptor mRNA in normal and malignant ovarian epithelial...

Ngày tải lên: 20/06/2014, 07:20

... the functional activities of GRIP1 They extend our understanding of the importance of the structural status of GRIP1 in modulating NR functions Results Autoregulation of GRIP1 AD activities by ... 10 WB anti-HuR Fig Modulation of glucocorticoid receptor- interacting protein (GRIP1) transactivation activity (A, B) Expression vectors (0.5 lg) for the indicated fragments of GRIP1 fused to the ... co-activation of GRIP1, using a series of C-truncations to monitor its co-activator functions in the androgen receptor (AR), estrogen receptor (ER) and thyroid receptor (TR) systems (Fig 6) Our previous...

Ngày tải lên: 07/03/2014, 12:20

... in an increase in the number of binding sites and a decrease of the affinity of the uPAR [38,39] The quantification of uPAR expression by measurement of the amount of uPA bound is thus not ideal ... and inducible gene expression including Ó FEBS 2002 uPA and uPAR possibly through suppression of upstream targets of ERK Thus, one of the functions of bikunin is the regulation of uPA synthesis ... PMA-induced uPAR expression shares the characteristics of that of uPA expression, since the increased uPAR expression is affected by PKC inhibitors (calphostin C and staurosporin) and the uPA expression...

Ngày tải lên: 17/03/2014, 11:20

... http://arthritis-research.com/content/11/4/R108 Figure Evaluation of FCM analysis of GR expression by western blot Analysis of GR repression in (left) K562 and (right) U937 with and without 10-6 M Dex of GR expression by western blot ... the expression and binding at the same time Moreover, the administration of GR agonists results in a significant down-regulation of the expression of GR in vitro and in vivo, so we detected GR of ... protein expression of cell surface receptors and has also been shown to be useful in identifying expression of intracellular proteins in permeabilised cells [36] Especially when complex mixtures of...

Ngày tải lên: 09/08/2014, 14:22

... inhibition of proliferation of VSMC, which is mechanistically linked to a KLF4-induced enhancement of the expression of the tumour suppressor gene p53 [5] Because of the important roles of KLF4 ... important role in the activation of endothelial cells and macrophages, as well as the differentiation and proliferation of VSMCs Overexpression of KLF4 induced expression of multiple anti-inflammatory ... that the promoter of SR-BI contained multiple putative KLF4 binding sites However, the direct effect of KLF4 on the expression of SR-BI remains unknown Here, the expression of KLF4 in response...

Ngày tải lên: 18/02/2014, 04:20

... present Foreign ADC gene expression in T cruzi work we used ADC-transgenic protozoa to follow the time-course modulation of foreign gene expression and to investigate whether this modulation can be ... partial digestion of the DNA samples On the other hand, the absence of a second hybridization band in lane might be the result of insufficient sensitivity of our assay Rehybridization of the membrane ... presence of different concentrations of a-amanitin Figure shows that transcription of the ADC gene did not decrease, even at very high levels of a-amanitin (500 lgÆmL)1), while the synthesis of cruzipain...

Ngày tải lên: 19/02/2014, 07:20

... the production of PGE2 [18] The inhibition of PGE2 release was the consequence of a reduction of COX-2 and secretory PLA2 mRNA expression Moreover, the IL-1b-induced expression of secretory PLA2 ... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... down-regulation of Egr-1 mRNA levels, which is observed in n-3 treated cells, also indicates that other binding elements may contribute to the modulation of egr-1 gene expression (Fig 4B) Influence of n-3...

Ngày tải lên: 19/02/2014, 16:20

... molting hormone receptor Here, we report (a) the determination of primary amino acid structures of EcR and USP from L decemlineata (b) the analysis of mRNA expression profile of L decemlineata, ... staining of rRNA is shown as a control for equal loading (B) The expression of LdEcR-A and LdEcR-B1 mRNA in integument of last instar larvae Temporal expression profiles were studied using isoform-specific ... decemlineata also possesses two isoforms, and the expression of LdEcR-A was much stronger than LdEcR-B1 (Fig 2A) Expression of EcR-A was also predominant in larval tissue of coleopteran T molitor [22]...

Ngày tải lên: 07/03/2014, 21:20

... vertebrates [812] The use of yeast systems to monitor the interaction of different chemicals with vertebrate receptors has become a common tool to detect the presence of receptor- binding activity ... slopes of the regression lines (- - -) Fig Doseresponse of BNF in the presence of increasing concentrations of carbaryl in pLMAX-RYA (A) Showing data relative to the maximal activity of BNF in ... characteristics of the receptor, but to the sensitivity of the particular cell strain used in the assay This effect can be monitored by measuring the effect of the compound to the activation of galactose-responsive...

Ngày tải lên: 16/03/2014, 10:20

... Inhibition of uPAR by DNAzymes all of which contribute to focal proteolysis and regulation of wound healing, angiogenesis, and metastasis [5] Of the components of the uPA system, uPAR is of particular ... absence of any energy source [15] This report outlines the design of novel DNAzymes that target uPAR mRNA as a method of inhibiting its expression and thereby decreasing the invasive potential of ... substrate, and a maximum reduction of 84% of uPAR substrate concentration at a molar ratio of : 200 (Fig 2D) The specificity of the DNAzymes was confirmed by the lack of cleavage by the untreated and...

Ngày tải lên: 16/03/2014, 22:20

... domain of the androgen receptor Biochemistry 36, 1052–1064 19 Ikonen, T., Palvimo, J.J & Janne, O.A (1997) Interaction between the amino- and carboxyl-terminal regions of the rat androgen receptor ... Identification of two transcription activation units in the N-terminal domain of the human androgen receptor J Biol Chem 270, 7341–7346 Ó FEBS 2002 Interaction between androgen receptor subdomains ... function of AR NTD and thus does not allow discrimination between loss of AR.NTD-AR.LBD binding and loss of AR.NTD transactivating function In the yeast assay, loss of transactivation function of AR...

Ngày tải lên: 17/03/2014, 10:20

... phase of adipogenesis [7] The regulation of adipogenesis by PGs is thus not yet fully understood In this study, we demonstrate the effect of PGF2a– FP receptor signaling on the expression of COX-2 ... suppression of the early phase of adipogenesis in mouse adipocytic 3T3-L1 cells PGF2a promoted the production of anti-adipogenic PGF2a and PGE2 by enhancing the expression of COX-2 through FP -receptor- activated ... positive feedback FP receptor- MEK ⁄ ERK-CREBCOX-2 loop Results Activation of FP receptor increases PGF2a production with enhanced expression of COX-2 During the adipogenesis of mouse 3T3-L1 cells,...

Ngày tải lên: 28/03/2014, 22:20

... water-soluble variant of RSL-1, namely RG-102277 First, we determined the effect of dimethylsulfoxide itself on the expression of genes in 293 cells The expressions of a total of 43 genes were modulated ... total of 20 lL reaction volume containing lL of cDNA, lL of each of forward and reverse sequence specific primers (from 10 lm primer stock), 10 lL of supermix (Bio-Rad Laboratories) and lL of nuclease ... in the movement of cilia In summary, the use of the THQ gene switch ligand RG-120499 may cause significant changes in the expression of host genes AMK, RG-121150, affects gene expression in 293...

Ngày tải lên: 30/03/2014, 03:20

... importance of Th1 cytokines in the pathogenesis of SLE-like disease in MRL/lpr mice, the specific profiles of IP-10, of ligand for chemokine receptor, and of CXCR3 of Th1 phenotype in various aspects of ... the pathogenesis of murine lupus, we examined the profiles of the expression of TARC (Th2 type chemokine) and its receptor, CCR4, in the lungs As shown in Fig 7, expression of TARC transcripts ... shown) We also examined the expression pattern of CXCR3 transcripts Interestingly, although a weak expression of CXCR3 transcript was seen in the lungs of B6 mice, the expression pattern in the...

Ngày tải lên: 09/08/2014, 01:23

... risk of breast cancer in women bearing a combination of large CAG and GGN repeats in the exon of the androgen receptor gene Eur J Cancer 2007, 43:2373-80 22 Wang W, John EM, Ingles SA: Androgen receptor ... development and growth of tumors in hormone dependent tissues especially breast The action of steroids is mediated by steroid hormone receptors, which are members of nuclear receptor family of ligand-activated ... ligand-activated transcription factors The role of androgens and androgen receptors in breast carcinogenesis is poorly understood, although wide spread expression of AR in breast cancer suggests that it...

Ngày tải lên: 09/08/2014, 03:21

... downregulation of A3AR expression level was noted in cells derived from the synovial tissue, most probably due to receptor internalization and degradation Subsequently, decreased levels of expression of ... We further examined the effect of MTX on expression of A3AR protein compared with A2AAR protein in paw extracts Figure 3d shows that the expression levels of both receptors is increased with MTX ... Effect of MTX, CF101 and MTX+ CF101 treatment on exhibition and expression of A3AR in paw and PBMCs from AIA rats Immunohistochemistry staining of paw sections derived from AIA rats revealed the of...

Ngày tải lên: 09/08/2014, 08:23

... CXCR3, CXCR4 or CXCR5, and the expression of the various markers was analyzed by flow cytometry CD19+ B cells were gated, and the frequency of expression of each chemokine receptor is shown Data represent ... the chemokine receptor expression of RA peripheral blood B cells was similar to that of healthy donors without any statistically significant differences As with healthy donors, CCR5 expression by ... frequent than that of CD27- B cells, and CXCR4 expression by CD27+ B cells was less frequent than that of CD27- B cells In addition, the proportions of CCR6, CCR7 and CXCR5 expression were significantly...

Ngày tải lên: 09/08/2014, 14:22

... determine whether hypoglycemia as a consequence of insulin therapy in diabetes altered the binding parameters of Muscarinic M1, M3 receptors and gene expression of α7nAchR, AchE, ChAT and GLUT3 in the ... regulation of Muscarinic M1, M3, α7nAchR, AchE, GLUT3 mRNA and down regulation of ChAT gene expression in cerebellum of Diabetic, Diabetic + IIH and Control + IIH Rat Real-time PCR analysis of Muscarinic ... al., Hypoglycemia induced changes in cholinergic receptor expression in the cerebellum of diabetic rats Journal of Biomedical Science 2010, 17:7 Page of ...

Ngày tải lên: 10/08/2014, 05:21

... antimelanogenic mechanism of Cpc The expression of tyrosinase and the production of melanin were determined to examine the antimelanogenic effect of Cpc The levels of signaling molecules such ... the level of MITF was thus investigated to characterize the antimelanogenic mechanism Figure 3C displays the expression profile of MITF proteins after Cpc treatment The expression of MITF protein ... detour of the stimuli, consequently leading to suppression of the activation of CREB Consequently, the expression of melanogenic enzymes (tyrosinase, TRP-1, DCT) is hampered due to the limited expression...

Ngày tải lên: 10/08/2014, 10:20