models gene expression and acgh

Báo cáo y học: ": Network analysis of skin tumor progression identifies a rewired genetic architecture affecting inflammation and tumor susceptibility" docx

Ngày tải lên : 09/08/2014, 22:23

... to elucidate the genetic architecture of cancer susceptibility A combination of genetic and gene expression analysis of human tumors will complement genetic association methods and may identify ... package [32] Materials and methods eQTL analysis Mouse models, gene expression, and aCGH Pairs of papillomas from the same animal were combined for eQTL analysis using the mean expression for each ... that gene copy number alterations on distal chromosome have disrupted the normal genetic control of expression of these target genes Genomic networks are rewired during tumorigenesis Changes in gene...

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Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

Ngày tải lên : 19/02/2014, 07:20

... discussions and Edith Trejo and Carlos Zadikian for technical assistance We are indebted to Drs J J Cazzulo and C Labriola for their generous gifts of a cruzipaincontaining plasmid and primers A and ... [10] In the present Foreign ADC gene expression in T cruzi work we used ADC-transgenic protozoa to follow the time-course modulation of foreign gene expression and to investigate whether this ... the parasite genome organization Results and Discussion Expression of the oat ADC gene in T cruzi In order to study the expression of the foreign ADC gene in T cruzi epimastigotes, we transformed...

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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt

Ngày tải lên : 21/02/2014, 01:21

... activation and NAMI-A- induced down regulation of c-myc gene expression NAMI-A inhibits c-myc gene transcription and protein expression To establish whether the decrease in c-myc mRNA expression ... regulates c-myc gene expression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in ... FEBS 2002 NAMI-A aﬀects c-myc gene expression and MEK/ERK phosphorylation (Eur J Biochem 269) 5867 Fig NAMI-A and PD98059 completely inhibits PMA-induced c-myc gene expression (A) Serum-starved...

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Plant physiology - Chapter 14 Gene Expression and Signal Transduction potx

Ngày tải lên : 07/03/2014, 21:20

... pathways in which one or Gene Expression and Signal Transduction (A) Tryptophan operon 5′ DNA Regulatory gene i Transcription Promoter p Operator o 3′ Gene E Gene D Gene C Gene B Gene A Transcription ... on other genes Other important protooncogenes that encode nuclear transcription factors include MYC and MYB Both phytochrome (see Chap- Gene Expression and Signal Transduction ter 17) and gibberellin ... discussion of eukaryotic gene expression with a brief overview of transcriptional regulation in prokaryotes However, it is now clear that Gene Expression and Signal Transduction gene regulation in...

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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

Ngày tải lên : 16/03/2014, 02:20

... regulating the gene expression of rate-limiting enzymes of gluconeogenesis and glycogenolysis, including phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), and by regulating ... cells, and the ensuing effect on gluconeogenesis and glucose output Our results show that, in the presence of TNFa, insulin-induced inhibition of gluconeogenesis and glucose output is attenuated and ... with TBP and b-actin antibodies and taken as nuclear and cytosolic loading controls, respectively, and also used to ascertain the purity of the nuclear and cytosolic preparations (A) Bands were...

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Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot

Ngày tải lên : 16/03/2014, 22:20

... breakdown of ECM and facilitate cell invasion and metastasis Increased expression of uPAR is associated with invasive cancer cell phenotypes and a poor prognosis The ability to decrease uPAR expression ... decreased [9] We have previously cloned the human uPAR gene and characterized its promoter and transcription factors, including two AP-1 and one NF-jB motifs [10–12] We have also found that colon ... uPAR expression in colon cancer invasion and metastasis [13] To develop a potential tool for uPAR gene therapy, we used a catalytic DNA enzyme (DNAzyme) approach The DNAzymes were originally generated...

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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx

Ngày tải lên : 18/03/2014, 01:20

... sensitive, and that the pA1lacO-1 promoter and the lacI q1 gene are surrounded by a DNA fragment originating from upstream the gyrA gene and a fragment containing the N-terminal part of the gyrA gene ... digested with the enzymes DraI and EcoRV and a 842-bp fragment containing the DNA region upstream of topA and the topA promoter was isolated and inserted into pUC19 (New England Biolabs) digested with ... were obtained from and used as recommended by New England Biolabs and Boehringer Mannheim Plasmid and ATP, ADP extraction Aliquots (0.8 mL) were removed from cell cultures and placed into an...

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Báo cáo khoa học: Bile acids increase hepatitis B virus gene expression and inhibit interferon-a activity pot

Ngày tải lên : 22/03/2014, 21:21

... metabolism and HBV gene expression H Y Kim et al the liver, intestine, kidney and adipose tissue via the regulation of the expression and function of genes involved in bile acid synthesis, uptake and ... metabolism and HBV gene expression Fig The effects of bile acids on HBV gene expression in hepatocyte cell lines (A) Chang liver, HepG2 and Huh7 cells were transfected with the 1.3x HBV-luc construct and ... metabolism and HBV gene expression Fig The effect of AP-1 and C ⁄ EBPs on bile acids-induced HBV gene expression (A) Chang liver cells were cotransfected with AP-1-luc or 3xC ⁄ EBP-luc construct and...

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Báo cáo khoa học: Glutamine stimulates the gene expression and processing of sterol regulatory element binding proteins, thereby increasing the expression of their target genes docx

Ngày tải lên : 28/03/2014, 22:21

... SREBP target gene, and that the transcription of SREBP-1a gene is predominantly regulated by the general transcription factor Sp1 [11,12] We next compared the glutamineinduced gene expression of ... of SREBP gene expression is not the initial trigger for the glutamine effects If induction of the SREBP gene expression serves as the initial trigger for the glutamine effects, the gene expression ... in the induction of SREBP-1a gene expression When endogenous Sp1 expression was reduced to 20% of normal with gene- speciﬁc small interfering RNA (siRNA), the gene expression of SREBP-1a was signiﬁcantly...

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Báo cáo khoa học: Selenium affects biosilica formation in the demosponge Suberites domuncula Effect on gene expression and spicule formation pot

Ngày tải lên : 30/03/2014, 20:20

... (2000) Expression of silicatein and collagen genes in the marine sponge Suberites domuncula is controlled by silicate and myotrophin Eur J Biochem 267, 4878–4887 Muller WEG (2005) Spatial and temporal ... Obrecht-Pfumio S, Krol A, Thisse B & Lescure A (2003) Spatial and temporal expression patterns of selenoprotein genes during embryogenesis in zebraﬁsh Gene Expr Patterns 3, 525–532 22 Pisera A (2003) Some ... PAGE As shown in Fig 3, the predominant band identiﬁed corresponds to a 14-kDa protein (lanes a and b) Weaker bands corresponding to sizes of 35 kDa and 23 kDa were also detected if larger amounts...

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Báo cáo khoa học: A functional polymorphism at the transcriptional initiation site in b2-glycoprotein I (apolipoprotein H) associated with reduced gene expression and lower plasma levels of b2-glycoprotein I docx

Ngày tải lên : 31/03/2014, 07:20

... [30,31] However, our in vitro mutagenesis and expression study did not link these mutations to an altered b2GPI gene expression [32] Although, in vitro mutagenesis and expression study not rule out ... mutation and is associated with significantly lower b2GPI plasma and mRNA levels and the low occurrence of APA Furthermore, this mutation reduces the expression of the luciferase (Luc) reporter gene ... genotype (lanes 1, and 4) Effect of the )1CfiA mutation on reporter gene expression To further confirm that the )1CfiA mutation is associated with low expression of the b2GPI gene, we performed...

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báo cáo hóa học: " Pro-inflammatory gene expression and neurotoxic effects of activated microglia are attenuated by absence of CCAAT/enhancer binding protein b" potx

Ngày tải lên : 19/06/2014, 22:20

... proinflammatory gene expression in C/EBPb-/- mixed glial cultures Expression of pro-inflammatory (NOS2, IL-1b, IL-6 and TNFa) and anti-inflammatory (IL-4 and TGFb1) genes in C/EBPb+/+ (white bars) and C/EBPb-/- ... the anti-inflammatory genes IL-4 and TGFb1 Absence of C/EBPb results in significant decreases in LPS-induced expression of NOS2 and in LPS+IFNg-induced expression of NOS2 and IL-1b Results are ... proinflammatory gene profiles and neurotoxicity in glial cultures from C/EBPb-null mice Our results show for the first time that absence of C/EBPb attenuates proinflammatory gene expression and abrogates...

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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc

Ngày tải lên : 20/06/2014, 01:20

... antiviral, IFN-stimulated genes (ISGs) As specific and sensitive markers we used the ISG56 gene which is induced both by IFNs and by virus infection [38,39] and the MxA gene which is exclusively ... cytokine-inducing viruses activated IP-10 and RANTES expression in HEK 293 cells as expected (Fig 4B, upper and lower panels) Thus, SARS-CoV induces IP-10 gene expression in Caco-2 cells, but not in ... study, and was responsible for drafting and finalizing the manuscript All authors read and approved the final manuscript Acknowledgements We thank Otto Haller for support and helpful comments, and...

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báo cáo hóa học:" Gene Expression and Pathway Analysis of Ovarian Cancer Cells Selected for Resistance to Cisplatin, Paclitaxel, or Doxorubicin" docx

Ngày tải lên : 20/06/2014, 08:20

... and identified gene expression patterns altered during the development of chemoresistance Among the genes that are consistently elevated we identify previously known genes such as ABCB1 and genes ... generate novel drug resistant ovarian cancer cell lines independently selected for resistance to cisplatin, doxorubicin or paclitaxel, and we use gene expression profiling to identify genes and ... of gene expression changes This suggested different mechanisms of resistance for the different drugs Comparison of gene expression between sensitive and resistant lines revealed numerous genes...

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Báo cáo y học: "Abnormalities of B cell phenotype, immunoglobulin gene expression and the emergence of autoimmunity in Sjögren’s syndrome" pdf

Ngày tải lên : 09/08/2014, 01:21

... of chemokines and their receptors [10–12] Cytokines and chemokines in pSS In patients with pSS the salivary and lacrimal glands are the main target organs for the immune system and, at least ... encoded by VκA2, O8/O18, L11, A17 and A27 [62] Moreover, Vλ genes of the Vλ2 and Vλ7 family were found in the Hib-antibody VL gene repertoire [62] In addition, VκA27 and Vλ2C, 2E, 2A2 or 10A were ... normal salivary glands It has therefore been suggested that T helper type (Th1) cytokines, such as IFN-γ and IL-2, as well as IL-10, IL-6 and TGF-β, might be important in the induction and/ or maintenance...

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Báo cáo y học: "Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis" pptx

Ngày tải lên : 09/08/2014, 06:23

... 0.01) and lumican (14.6-fold; P < 0.01) In the same region there were downregulations of decorin (1.6-fold; P < 0.01) and CTGF (2.1-fold; P < 0.05), and unchanged expression of fibromodulin and ... University for her assistance with the animal handling and care 22 Maroudas AI: Balance between swelling pressure and collagen tension in normal and degenerate cartilage Nature 1976, 260:808-809 ... health and osteoarthritis Evidence for molecular heterogeneity and extensive molecular changes in disease J Clin Invest 1992, 90:2268-2277 Matyas JR, Adams ME, Huang D, Sandell LJ: Discoordinate gene...

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Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx

Ngày tải lên : 09/08/2014, 06:23

... of both MMP-13 mRNA expression and the protein to an approximately similar extent Cathepsin K gene expression and protein synthesis The levels of both the gene expression and the protein of cathepsin ... collected and processed with GeneAmp® 5700 SDS software and given as a threshold cycle (Ct) Plasmid DNA containing the target gene sequences was used to generate standard curves A DNA standard curve ... correlation exists between the mRNA and protein levels At the two dosages tested, the R1094 ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis The level of gene expression of ADAMTS-5 in OA...

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Báo cáo y học: "Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblast" potx

Ngày tải lên : 09/08/2014, 14:20

... samples HexA and HexB genes were characterized by the second strongest gene expression in all samples (Figure 1) The expression of HexA gene was approximately the same both in SFs and in SMs In ... subunit (HexB) gene was characterized by significantly higher gene expression in RA and OA SFs as compared with RA and OA SM tissue samples (Mann – Whitney rank sum test) Gex, gene expression; HGPRT, ... 100% As shown in Figures 5a and 6a, TGF-β1 has significantly downregulated the expression of HexA and HexB genes, as well as of GusB and Hc-gp 39 The suppression of gene expression was more pronounced...

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Báo cáo y học: "JNK pathway is involved in the inhibition of inflammatory target gene expression and NF-kappaB activation by melittin" ppsx

Ngày tải lên : 11/08/2014, 08:22

... and COX-2 expression, and on NO and PGE2 generation To investigate whether the suppressed effect of SP600125 on the inhibitory effect of bee venom and melittin on the inflammatory gene expression, ... expression, iNOS and COX-2 expression was determined The inhibitory effect of melittin and bee venom on iNOS and COX-2 expression by LPS and SNP in Raw 264.7 cells (Fig 5A) and in synoviocytes ... melittin and bee venom on the inflammatory gene expression induced by LPS or Figure SNP JNK inhibitor suppressed inhibitory effect of melittin and bee venom on the inflammatory gene expression...

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báo cáo khoa học: " Gene expression and hypoxia in breast cancer" doc

Ngày tải lên : 11/08/2014, 12:21

... modulates mitochondrial function and decreases ISCU and COX10 expression Oncogene 2010, 29:4362-4368 doi:10.1186/gm271 Cite this article as: Favaro E, et al.: Gene expression and hypoxia in breast cancer ... hypoxia gene- expression signature in treatment response [47] This highlights the need for more comprehensive studies to investigate the expression of multiple hypoxia markers and of gene and miRNA ... (that is, by finding) genes whose expression pattern was similar to that of a set of well-known hypoxia-regulated genes, including CAIX, GLUT1 and VEGF The metagene contained 99 genes, several of...

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