mitotyping sequencing and analyses of mitochondrial dna and nuclear dna

... three of them (COX1, COX3, and CYTB) also lead to mitochondrial dysfunction Also worth noting is that repairs of UV-induced DNA damage by ERCC2/XPD and ERCC5/XPG requires ATP The ATP and DNA binding ... Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus Lupus 2008, 17:988-995 Fernandez D, Perl A: Metabolic control of T cell ... of new mitochondrial components, while nuclear respiratory factor functions as a transcription factor that activates the expression of certain nuclear genes required for mtDNA transcription and...

Ngày tải lên: 12/08/2014, 15:23

... the suggestion of a different origin of wild boars in Japanese islands and Ryukyu islands [45] In the phylogenetic tree of complete mtDNA sequences, all East Asian domestic pig mtDNAs were clustered ... median network of domestic pig and wild boar mitochondrial DNAs (mtDNAs) belonging to haplogroup D1 based on the sequence variation of control region and coding region fragments The mtDNA control ... on mainland Japan [50] Evidence of the origin of Japanese domestic pigs was mainly estimated from cultural records and ancient DNA studies Recent studies of ancient DNA conducted in pig and wild...

Ngày tải lên: 14/08/2014, 08:20

... the data set of Weiss et al [3] including 58 haplotypes plus three new sequences of the 3’end of the 595 bp fragment of mtDNA CR and 74 bp of flanking tRNA (Phe) from the Serbian part of Danubian ... Fermentas Taq polymerase and μL of template DNA (~100 ng of genomic DNA) ; the cycle parameters were as follows: initial denaturation (95°C, min) followed by 32 cycles of strand denaturation (95°C, ... electrofishing and angling between 2007 and 2008 (Table and Figure 1) Fin clips were sampled and stored in 96% ethanol Total DNA was isolated from this tissue using the Wizard Genomic DNA Purification...

Ngày tải lên: 14/08/2014, 13:21

... Resistance of mitochondrial DNA- depleted cells against cell death: role of mitochondrialsuperoxide dismutase J Biol Chem 279, 7512–7520 17 Chandel, N.S & Schumacker, P.T (1999) Cells depleted of mitochondrial ... exception of catalase and GPx activity, depletion of mtDNA diminished SOD and GST activities in boneand muscle-derived q0 cells but not in lung-derived q0 cells (Figs 3–6), where SOD (Figs 3–5) and ... 15 Jazayeri, M., Andreyev, A., Will, Y., Ward, M., Anderson, C.M & Clevenger, W (2003) Inducible expression of a dominant negative DNA polymerase-c depletes mitochondrial DNA and produces a q0...

Ngày tải lên: 16/03/2014, 18:20

... amplification across the junctions of mitochondrial and unique nuclear DNA, followed by the sequencing of the corresponding fragments [25] This organellar-to -nuclear transfer isochore is indicated ... orange dots in Fig 1, and they are located in 0–230 kb of chromosomes II and 0–350 kb of chromosomes IV, respectively The similar genomic organization of chromosomes I, III and V makes their overall ... arms and often most abundant in percentromeric DNA [16] The uninterrupted repeat arrays may up to more than Mb in the centromere region of each chromosome [23] and the unsequenced regions of centromeres...

Ngày tải lên: 30/03/2014, 20:20

... (plasma-5) and 12 (SF-5) were spiked with 10 ng of purified mtDNA Statistical analyses Figure Differences between the groups in the levels of PCRamplified mitochondrial DNA in plasma and SF, and in ... presence of free mtDNA and 8-oxodG in the SF of patients with RA and the appearance of other laboratory parameters, such as RF, erosivity, and extra-articular manifestations The presence of both mtDNA ... to mitochondrial and nuclear DNA is extensive Proc Natl Acad Sci USA 1988, 85:6465-6467 Yakes FM, Van Houten B: Mitochondrial DNA damage is more extensive and persists longer than nuclear DNA...

Ngày tải lên: 09/08/2014, 01:23

... procedures, and performed clinical and rheumatological data analyses AZ, CB and EC conducted assessment of Chlamydia trachomatis serology and DNA extraction BJ and JS participated in the design and coordination ... the role that this variety of intraarticular bacterial DNA plays in the pathogenesis of ReA and other forms of arthritides However, detection of bacterial DNAs in the ST of patients with arthritis ... DNA in patients with ReA and UA using broad-range PCR, cloning and sequencing of almost the entire 16S rRNA gene The use of this approach revealed the identity of potential bacterial causes and...

Ngày tải lên: 09/08/2014, 10:23

... broad-range PCR, cloning and sequencing the entire 16S rDNA and demonstrated the presence of a large number of bacterial DNA in the synovial tissue (ST) of patients with ReA, UA, and other arthropathies ... until analysis Automated DNA extraction from synovial fluid and broad-range PCR amplification of 16S rRNA genes, cloning and sequencing of bacterial DNA For 10 minutes, 500 μl of SF were centrifuged ... PCR as well as cloning and sequencing of the entire 16S rDNA to identify any bacterial DNA potentially present in the SF samples of patients with ReA, UA, and other forms of arthritis who were...

Ngày tải lên: 09/08/2014, 14:22

... Genotype A and D strains of HBV On the other hand, despite being costly or technically demanding, LiPA and direct DNA sequencing yielded accurate identification of all genotype A and genotype D and ... primers specific for both, genotype A and genotype D, and were thus identified as mixed genotype A and D strains The DNA sequencing data of genotype-specific region of HBV polymerase gene correlated ... Alignment of nucleotide sequences of the S-gene product Representative sequence of genotypes A (X75669) and D (X65259) are shown at the bottom of the raw Inherent recognition sites of Nla IV...

Ngày tải lên: 12/08/2014, 04:20

Ngày tải lên: 14/11/2014, 06:05

... and acts as a promoter for both the heavy and light strands of the mtDNA, and contains essential transcription and replication elements The D-loop region is a hot spot for mtDNA alterations and ... amplification, sequencing and comparison of various mtDNA segments The primers for mtDNA D-Loop region were synthesized by Shanghai Sangong Co., Ltd (Table 1) and a total of 50 μl of mixture used ... Mutation of 303PolyC in the mtDNA D-loop region a: Sequence of 303PolyC in the mtDNA D-loop region of AGS cells, the section of black line is the normal sequence; b: Sequence of 303PolyC in the mtDNA...

Ngày tải lên: 25/10/2012, 11:18

... are suggestive of DNA damage as a cause of NS1-induced apoptosis ATM principally binds to free DNA ends or DNA strand breaks (43), while ATR recognizes single-stranded regions of DNA common to ... cellular DNA, and that this damage leads to apoptosis Upon detection of DNA damage, DNA damage response proteins inhibit the cell cycle and are capable of inducing apoptosis if the DNA lesion ... in a covalent manner, and that both DNA- helix distorting and single strand nick forms of DNA damage are important pathways to apoptosis upon expression of NS1 Materials and Methods Transfection...

Ngày tải lên: 25/10/2012, 11:18

... allow linear accumulation of one strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro expression of PCR products 99 Template DNA PCR with one biotinylated primer ... quantity of molecular size markers Estimate the quantity of sample DNA by comparing the relative intensity of the ethidium bromidestained band with the marker bands A total of 30–90 ng of DNA is ... purification of the DNA Treatment with alkali leads to dissociation of the nonbiotinylated single strand Both the strands can be used as templates for DNA sequencing reactions The immobilized strand can...

Ngày tải lên: 25/10/2013, 22:20

... (1969) Nuclear magnetic resonance studies of the structure and binding sites of enzymes Inhibitor binding to ribonucleases J Mol Biol 45, 491511 33 Ui N (1971) Isoelectric points and conformation of ... Fc| RhFo, where Fo and Fc are the observed and calculated structure factor amplitudes of reection h, respectively Rfree is equal to Rcryst for a randomly selected 5.0% subset of reections not used ... Widlanski TS (1994) Synthesis of sulfonate-linked DNA J Org Chem 59, 3520 3521 McElroy EB, Bandaru R, Huang J & Widlanski TS (1994) Synthesis and physical properties of sulfonamidecontaining oligonucleotides...

Ngày tải lên: 14/02/2014, 22:20

... researchers and analysts engaged in analyses of military enlistment behavior This research was sponsored by the Assistant Secretary of the Army (Manpower and Reserve Affairs) and was conducted in RAND ... relative costs of producing the two types of contracts Equity in missioning is important in and of itself and because if recruiters perceive unfairness, it could undermine morale and effort and thereby ... because of pure chance The analogous proportions for stations failing and 12 months in a row are 82 and 23 percent The analyses reported in Chapters Four and Five develop and implement new models and...

Ngày tải lên: 17/02/2014, 22:20

... component command and a number of transportation management officers at bases and stations Many of the business rules documented here first came from these interviews At RAND, we thank John Ausink and Nancy ... programs (and socioeconomic goals) 14 Estimating DoD Transportation Spending: Analyses of Contract and Payment Transactions This work is a direct outgrowth of RAND research on the implementation of ... different types of actions and data entry rules in making transportation purchases This lack of standardization in data systems and procurement processes leads to gaps and overlaps among and between...

Ngày tải lên: 17/02/2014, 23:20

... branchial plume cDNA with an open reading frame of 726 nucleotides and 5¢- and 3¢ UTR sequences of 171 and 442 nucleotides, respectively Positions of the primers on the complete cDNA are given in ... Sciences EMS), covered with coverslips and sealed with nail varnish Homologies search, alignment, and phylogenetic analyses blast analyses (blastx and blastn) of the cDNA libraries sequences were conducted ... previously sequenced RpCAtr (BPNJ ¼ 100 and BPMP ¼ 99) Fungia scutaria (FCA-a and FCA-b) and Caenorhabditis elegans (CA1 and CA2) sequences fall outside of clade I and are more closely related to each...

Ngày tải lên: 18/02/2014, 16:20

... gene sequencing, N-terminal sequencing and crystallization of mitochondrial complex II from porcine heart Characterization of respiratory complex II be the case that some indispensable cofactors ... Characterization of respiratory complex II Fig Sequencing of porcine heart mitochondrial complex II Total RNA was extracted and purified from the fresh porcine heart, and genes of four complex ... band is not very clear in the agarose gel, and the longest band was used to run another PCR reaction for amplification Experimental procedures Extraction, purification and crystallization of mitochondrial...

Ngày tải lên: 19/02/2014, 02:20

... in intramitochondrial and extramitochondrial ATP concentrations and Dw [16] The relative contribution of a particular enzyme to the control of metabolic pathway flux and concentrations of reaction ... because of inadequate expression of the latter [7] LCACs modulate the activity of the mitochondrial adenine nucleotide translocator (ANT) from both the outer and matrix sides of the inner mitochondrial ... Palmitoyl-CoA and control of mitochondrial function J Ciapaite et al Control of the QH2/Q ratio and Dw Coenzyme Q reduction level and Dw are among the factors that determine ROS production by the mitochondrial...

Ngày tải lên: 19/02/2014, 05:20

... mtDNA in mitochondria or cells The reasons for the lack of alkylation of mtDNA within mitochondria by mitoDC-81 are unclear The local concentrations of mitoDC-81 and DNA, and the duration of ... to the mitochondrial membrane potential [11] From the known plasma and mitochondrial membrane potentials and the cell and mitochondrial volumes of 143B cells [48] we estimate an intramitochondrial ... reagent might also be useful in investigating the rate of turnover of mtDNA and in the derivation of mtDNA-free (q°) clones of the large number of cell lines where the classical treatment with ethidium...

Ngày tải lên: 20/02/2014, 11:20