... the BRD Exp media 155 was used, we managed to obtain an active protein ladder 25 0 150 100 75 50 Purified BRD ladder Purified RDR 25 0 98 75 64 36 37 25 22 Figure 2. 1 SDS-PAGE of purified N-histag ... 117 5.2. 2 In vitro Kinetic Analysis 118 5.2. 3 Biohydroxylation of Mutant P 450 s with Different Substrates 1 25 5. 3 Discussion 129 5. 4 Conclusion and ... Chapter 5 : Further Characterization of P 450 pyr and Related Mutants 1 15 5. 1 Introduction 1 15 5.2 Results 117 5.2. 1 Cloning, Expression, and Purification of WT P 450 pyr...
... the rodent GI tract 17 18 19 20 21 22 23 24 25 26 27 28 29 30 M Westerlund et al mechanism, functional roles and medical relevance Chem Biol Interact 143–144, 21 9 22 7 Vasiliou V, Bairoch A, Tipton ... CL, Lee SL & Han CL (20 03) Human class IV alcohol dehydrogenase: kinetic FEBS Journal 27 4 (20 07) 121 2– 122 3 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 122 1 Expression of ADH and ALDH in ... proliferation [26 ] Expression of Adh1 in deeper layers of the FEBS Journal 27 4 (20 07) 121 2– 122 3 ª 20 07 The Authors Journal compilation ª 20 07 FEBS M Westerlund et al Fig Schematic drawings of the stomach...
... Molecular cloning and expression of cDNA 16 17 18 19 20 21 22 23 24 25 26 27 28 29 encoding 3a,7a,12a-trihydroxy-5b-cholestanoyl-CoA oxidase from rabbit liver J Biol Chem 27 2, 18481–18489 Zhang, Y.X., ... carried out with i3VD [24 ], SCAD [27 ], and i2VD [29 ], demonstrating that the catalytic residues, Glu 25 4 in i3VD, Glu368 in SCAD, and Glu381 in i2VD are homologs of Glu 25 4 in LCAD and Glu376 in ... structures of the wild type and the Glu376Gly/ Thr 25 5 Glu mutant of human medium-chain acyl-CoA dehydrogenase: influence of the location of the catalytic base on substrate specificity Biochemistry 35, 124 12 124 20...
... 15 +/- 12 +/- 9.8 +/- 0 .2 18 +/- 9.7 +/- 1.9 12 +/- 12 +/- 12 +/- 12 +/- 10 +/- 18 +/- 9.8 +/- 0.8 23 +/- 22 +/- 29 +/- 16 +/- 17 +/- 35 +/- 10 12 +/- 22 +/- 15 +/- +/- +/- +/- 28 +/- 20 +/- SUBSTRATES ... concentrations of 50 .0 mM, 100.0 mM, 150 .0 mM, 20 0.0 mM, and 2 25 . 0 A 50 mM pentanol stock solution was used to create pentanol experiments at concentrations of2 .5 mM, 10.0 mM, 25 . 0 mM, 35. 0 mM, and 45. 0 ... concentrations of 0 .50 mM, 1 .5 mM, 3.0 mM, 5. 0 mM, and 5.5 mM A mM trans-2hexen-1-ol solution was used to create trans -2- hexen-1-ol experiments at concentrations 28 of 0.0 15 mM, 0. 02 mM, 0.0 25 mM, and...
... 54 Characterization of the enhancer element 4E 58 2. a Effect of 4E on heterologous promoters 58 2. b Function of 4E in non-hepatoma cells 58 2. c Localization of sequences required ... Validation of differential gene expression by qRT-PCR 51 11 Validation of alternative splicing by qRT-PCR 52 III.RESULTS 54 Identification of an enhancer in the ADH cluster 54 ... 20 Alcohol and the liver 21 10 Alternative transcript isoforms and diseases 24 11 Global transcriptional profiling 27 12 Research objectives 32 viii...
... 12 13 – 14 15 – 16 17 – 18 19 – 20 21 – 22 23 – 24 25 26 – 27 28 29 – 30 31 – 32 33 – 34 35 – 36 37 – 38 39 40 41 – 42 43 44 – 45 46 47 48 – 49 50 – 51 52 – 53 54 55 – 56 57 58 – 59 60 – 61 62 ... smart _ 23 Brenda's new boyfriend is very good looking, but he's not very intelligent or interesting He's just a bit of eye _ 10 11 12 13 14 16 19 17 18 20 21 22 23 24 25 26 22 15 Unit 'get' ... I I 21 P I 22 24 25 T O 26 28 30 A E 29 O U Y 20 R D L 27 E Z 31 32 Y N I 23 H 19 G 20 17 E Idioms and other expressions using food and drink Complete these sentences with items of food or drink,...