microbiology national university of singapore 5 science drive 2 singapore 117597
Engineering of an efficient and enantioselevtive biocatalyst for the preparation of chiral pharmaceutical intermediates
... the BRD Exp media 155 was used, we managed to obtain an active protein ladder 25 0 150 100 75 50 Purified BRD ladder Purified RDR 25 0 98 75 64 36 37 25 22 Figure 2. 1 SDS-PAGE of purified N-histag ... 117 5. 2. 2 In vitro Kinetic Analysis 118 5. 2. 3 Biohydroxylation of Mutant P 450 s with Different Substrates 1 25 5. 3 Discussion 129 5. 4 Conclusion and ... Chapter 5 : Further Characterization of P 450 pyr and Related Mutants 1 15 5. 1 Introduction 1 15 5. 2 Results 117 5. 2. 1 Cloning, Expression, and Purification of WT P 450 pyr...
Ngày tải lên: 12/09/2015, 10:22
Tài liệu Báo cáo khoa học: Medium-chain dehydrogenases/reductases (MDR) Family characterizations including genome comparisons and active site modelling pdf
... 7.0 8.0 7 .5 6.7 8 .5 5.9 21 2 173 144 25 7 21 1 22 6 24 4 21 0 21 0 22 6 22 6 176 20 7 20 7 21 2 2 05 20 2 1 45 188 77 )130 CSIHN-HEVVEIFRLN 0. 05 16.0 6.8 161 )133 CSRAH-HEFSEVTWMN )0.71 12. 4 8.6 22 0 Cinnamyl-alcohol ... 0.14 0 .26 0 .26 0.03 )0.11 )0.11 )0 .24 )0.68 )1 .28 0 .59 0.77 )0.34 13 .2 16.7 16 .2 15. 8 16.4 16.8 15. 8 16.9 15. 9 14.6 14.6 12. 9 12. 9 13.3 12 .5 12 .5 13 .2 9.7 12. 1 10 .2 7.0 7 .2 5. 9 9.1 7 .2 7.8 7.6 ... 7.6 6.9 6.3 21 1 160 22 2 188 168 25 2 161 23 4 Width ˚ (A) Volume ˚ (A3) Protein Annotation CE_F39B2–3 HS_QOR_HUMAN AT_T5P19–110 HS_QORL_HUMAN AT_K15M2 24 AT_F5O8 27 AT_F25G13–100 DM_ 72 958 51 Quinone...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
... of NADH independently of pH The Km of LDHB for NADH increased substantially FEBS Journal 27 4 (20 07) 59 24 59 36 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 59 25 Lactacte dehydrogenases of ... synthesis: phosphofructokinase, pyruvate kinase, and lactate dehydrogenase J Bacteriol 1 75, 25 4 1– 25 5 1 FEBS Journal 27 4 (20 07) 59 24 59 36 ª 20 07 The Authors Journal compilation ª 20 07 FEBS P Gaspar ... H (20 04) Effect of pyruvate kinase overpro- FEBS Journal 27 4 (20 07) 59 24 59 36 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 59 35 Lactacte dehydrogenases of Lactococcus lactis 29 30 31 32...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx
... the rodent GI tract 17 18 19 20 21 22 23 24 25 26 27 28 29 30 M Westerlund et al mechanism, functional roles and medical relevance Chem Biol Interact 143–144, 21 9 22 7 Vasiliou V, Bairoch A, Tipton ... CL, Lee SL & Han CL (20 03) Human class IV alcohol dehydrogenase: kinetic FEBS Journal 27 4 (20 07) 121 2– 122 3 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 122 1 Expression of ADH and ALDH in ... proliferation [26 ] Expression of Adh1 in deeper layers of the FEBS Journal 27 4 (20 07) 121 2– 122 3 ª 20 07 The Authors Journal compilation ª 20 07 FEBS M Westerlund et al Fig Schematic drawings of the stomach...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Prediction of coenzyme specificity in dehydrogenases ⁄ reductases A hidden Markov model-based method and its application on complete genomes doc
... Total 26 38 31 95 10 13 25 4793 4769 4774 4694 0. 929 0.7 92 0.7 05 0.7 92 1.000 0.999 0.999 0.999 4 821 4 821 4 821 4 821 FEBS Journal 27 3 (20 06) 1177–1184 ª 20 06 The Authors Journal compilation ª 20 06 ... 4 92 1 05 5 3 2 18 15 12 10 17 14 12 12 12 8 10 17 10 11 13 10 11 5 8 11 82 74 72 49 45 45 40 30 FEBS Journal 27 3 (20 06) 1177–1184 ª 20 06 The Authors Journal compilation ª 20 06 FEBS Y Kallberg ... of the coenzyme binding site location The numbers include all 68 investigated genomes Coenzyme 1N TM 2N TM 1C TM 2C TM >2 TM All FAD NAD NADP Dual Total 25 71 100 17 21 3 27 17 57 87 1 15 1 42 56 ...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khóa học: Acyl-CoA dehydrogenases and acyl-CoA oxidases Structural basis for mechanistic similarities and differences pot
... Molecular cloning and expression of cDNA 16 17 18 19 20 21 22 23 24 25 26 27 28 29 encoding 3a,7a,12a-trihydroxy-5b-cholestanoyl-CoA oxidase from rabbit liver J Biol Chem 27 2, 18481–18489 Zhang, Y.X., ... carried out with i3VD [24 ], SCAD [27 ], and i2VD [29 ], demonstrating that the catalytic residues, Glu 25 4 in i3VD, Glu368 in SCAD, and Glu381 in i2VD are homologs of Glu 25 4 in LCAD and Glu376 in ... structures of the wild type and the Glu376Gly/ Thr 25 5 Glu mutant of human medium-chain acyl-CoA dehydrogenase: influence of the location of the catalytic base on substrate specificity Biochemistry 35, 124 12 124 20...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khóa học: Genetic defects in fatty acid b-oxidation and acyl-CoA dehydrogenases Molecular pathogenesis and genotype–phenotype relationships ppt
... – 13 [55 ] 12 – 34 – – – – – – – – – – – – – – 20 1 – – – – – – – – – 1.7 0.9 2. 3 2. 0 2. 0 2. 3 1.4 1.3 0.9 1 .2 10 18 20 20 16 12 10 25 . 8 16 .5 [81] 60.03 17.8 5. 3 54 .0 45. 5 38.0 45. 5 13 15q23 19q13 ... 17.8 5. 3 54 .0 45. 5 38.0 45. 5 13 15q23 19q13 4q 32 5q33 3p21 11q13 22 q13 17p11 2p23 2p23 1p31 4q 22 12q 22 CAT SLC22A5 CACT SLC25A2 CPTI CPT1A CPTII CPT2 VLCAD ACADVL LCHAD HADHA LCKAT HADHB MCAD ACADM ... Dewar, K., Doyle, M., FitzHugh, W., 17 18 19 20 21 22 23 24 25 et al (20 01) Initial sequencing and analysis of the human genome Nature 409, 860– 921 Venter, J.C., Adams, M.D., Myers, E.W., Li,...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Inhibition kinetics of catabolic dehydrogenases by elevated moieties of ATP and ADP – implication for a new regulation mechanism in Lactococcus lactis potx
... AMP 2 .55 1.90 1 .56 1.64 4.61 1. 45 3.43 2. 03 0.96 0 .27 KIU 0 .54 0 .20 1.48 0 .51 0.34 0 .20 0.78 0.36 0 .26 0 .26 n 4.16 0. 92 5. 38 2. 76 5. 21 5. 69 1. 15 1. 75 0.94 0.94 4. 02 1. 42 1 .55 ... inhibition term 150 Vi/Vo 0. 75 0 .5 0. 25 0 ATP + ADP pool (mM) 12 100 50 15 2 Inhibitor (mM) 10 B B 25 0 Multiple inhibition term Vi/Vo 0. 75 0 .5 0. 25 20 0 150 100 50 0 ATP + ADP pool (mM) 12 15 Fig Multiple ... 0.9 750 0.9 920 0.9808 0.9888 0.3743 0 .54 49 0.0 020 0. 653 3 0 .22 27 0.6 720 0.88 92 0.00 15 0.0091 0.00 15 Set as a xed value as determined in one of the other assays FEBS Journal 27 7 (20 10) 18431 8 52 ê 20 10...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo hóa học: " Genetic variation and recombination of RdRp and HSP 70h genes of Citrus tristeza virus isolates from orange trees showing symptoms of citrus sudden death disease" potx
... Journal 20 08, 5: 9 http://www.virologyj.com/content /5/ 1/9 A B RM1 RM2 RM3 C3 RM4 Japan (AB046398) Spain (DQ 151 548) Israel (AY206 4 52 ) R1a C5 RM5 RM6 C6 RM7 US (AF001 623 ) Spain (Y18 420 ) US (AF260 651 ) ... (AF260 651 ) R1b C R2 R3 US (U16304) US (NC 001661) Egypt (AY340974) Mexico (DQ27 25 7 9) Spain (AY170468) R4 % of population 100 80 60 40 20 26 5 20 29 26 90 15 44 31 C3 C5 C6 0. 05 Figure Phylogenetic ... number not for citation purposes) Virology Journal 20 08, 5: 9 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Román MP, Cambra M, Juarez J, Moreno P, Vila ND, Tanaka FAO, Alves E,...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo lâm nghiệp: "Changing electrophoretic patterns of glutamate dehydrogenases and aspartate aminotransferases in a few tree species under the influence of ectomycorrhization" potx
... Biochem 26 , 17 -28 Kusnan M.B., Berger M.G & Fock H.P (1987) The involvement of glutamine synthetase/glutamate synthase in ammonia assimilation by Aspergillus nidulans J Gen Microbiol 123 , 12 35- 124 2 ... (1986) Assimilation of 15 by beech + NH (Fagus sylvatica L.) ectomycorrhizas New Phytol 1 02, 85- 94 Pateman J.A & Kinghorn J.R (19 75) Nitrogen metabolism In: The Filamentous Fungi Vol 2, (Smith J.E ... plants Nature 25 1 , 614-616 References Blumenthal K.H & Smith E.L (1973) Nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase of Neurospora J Biol Chem 24 8, 60 02- 6008 Martin...
Ngày tải lên: 09/08/2014, 04:20
KINETIC ANALYSIS OF PRIMATE AND ANCESTRAL ALCOHOL DEHYDROGENASES
... 15 +/- 12 +/- 9.8 +/- 0 .2 18 +/- 9.7 +/- 1.9 12 +/- 12 +/- 12 +/- 12 +/- 10 +/- 18 +/- 9.8 +/- 0.8 23 +/- 22 +/- 29 +/- 16 +/- 17 +/- 35 +/- 10 12 +/- 22 +/- 15 +/- +/- +/- +/- 28 +/- 20 +/- SUBSTRATES ... concentrations of 50 .0 mM, 100.0 mM, 150 .0 mM, 20 0.0 mM, and 2 25 . 0 A 50 mM pentanol stock solution was used to create pentanol experiments at concentrations of 2 .5 mM, 10.0 mM, 25 . 0 mM, 35. 0 mM, and 45. 0 ... concentrations of 0 .50 mM, 1 .5 mM, 3.0 mM, 5. 0 mM, and 5. 5 mM A mM trans-2hexen-1-ol solution was used to create trans -2- hexen-1-ol experiments at concentrations 28 of 0.0 15 mM, 0. 02 mM, 0.0 25 mM, and...
Ngày tải lên: 24/08/2014, 12:25
TRANSCRIPTIONAL REGULATION OF THE HUMAN ALCOHOL DEHYDROGENASES AND ALCOHOLISM
... 54 Characterization of the enhancer element 4E 58 2. a Effect of 4E on heterologous promoters 58 2. b Function of 4E in non-hepatoma cells 58 2. c Localization of sequences required ... Validation of differential gene expression by qRT-PCR 51 11 Validation of alternative splicing by qRT-PCR 52 III.RESULTS 54 Identification of an enhancer in the ADH cluster 54 ... 20 Alcohol and the liver 21 10 Alternative transcript isoforms and diseases 24 11 Global transcriptional profiling 27 12 Research objectives 32 viii...
Ngày tải lên: 24/08/2014, 12:37
EMBEDDED LINUX SYSTEM DESIGN AND DEVELOPMENT.pdf
... 144 5. 3.1 I2C Bus 1 45 5.3 .2 I2C Software Architecture 147 5. 4 USB Gadgets 1 52 5. 4.1 USB Basics 153 5. 4 .2 Ethernet Gadget Driver 158 5. 5 Watchdog Timer ... Architecture 32 2 .2. 1 Hardware Abstraction Layer (HAL) 33 2. 2 .2 Memory Manager 33 2. 2.3 Scheduler 34 2. 2.4 File System 35 2. 2 .5 IO Subsystem 36 2. 2.6 Networking ... Handler 134 5. 1 .5 Terminos Settings 138 5. 2 Ethernet Driver 138 5. 2. 1 Device Initialization and Clean-Up 140 5. 2. 2 Data Transmission and Reception 1 42 5. 3 I2C Subsystem...
Ngày tải lên: 04/08/2012, 14:23
09 Physical and Chemical Characteristics of DDGS revisions.
... 8b 10 12 13 14 15 16 Average Particle Size Mean 1398 1 322 14 25 1370 1 25 5 6 12 974 1 25 8 11 42 1337 1488 12 35 1198 122 9 21 25 1148 128 2. 25 Standard Deviation 2. 32 2.00 1. 62 1.84 1.68 2. 75 2. 15 1.70 ... 0 .20 0. 15 68% of the particles will fall between: 603 324 3 661 26 44 880 23 09 7 45 25 2 1 747 21 08 22 3 1683 453 20 94 740 21 39 621 21 01 751 23 80 919 24 11 706 21 61 641 22 40 58 8 25 6 9 13 62 33 15 510 25 8 3 ... 10.71 10.76 10.93 11. 02 11 .28 11.16 11.70 11.88 12. 13 12. 26 27 .60 27 .61 27 .59 27 .63 27 . 62 27.73 27 .71 27 . 62 27 .54 27 .61 27 .63 27 .61 27 .50 27 .53 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00...
Ngày tải lên: 08/08/2012, 10:03
CHECK YOUR ENGLISH VOCABULARY FOR BUSINESS AND ADMINISTRATION.pdf
... Title: 1 2 3 5 6–7 – 10 11 – 12 13 – 14 15 16 – 17 18 – 19 20 – 22 23 – 24 25 – 27 28 29 – 30 31 32 – 34 35 36 – 38 39 40 – 41 42 – 43 44 – 45 46 47 – 48 49 – 50 51 – 52 53 – 54 55 – 56 57 – 58 59 ... letter u) 55 JIT production = Just-in- _ production For reference see Dictionary of Business 4th edition (A&C Black 978-0-713-67918 -2) 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ... 29 30 31 32 33 34 35 36 37 38 39 40 42 41 43 45 44 46 47 48 49 50 51 52 53 54 55 56 For reference see Dictionary of Business - 4th edition (A&C Black Publishers Ltd, 978-0-713-67918 -2) Appraisals,...
Ngày tải lên: 08/08/2012, 17:31
Check Your English Vocabulary for Phrasal Verbs and Idioms.pdf
... 12 13 – 14 15 – 16 17 – 18 19 – 20 21 – 22 23 – 24 25 26 – 27 28 29 – 30 31 – 32 33 – 34 35 – 36 37 – 38 39 40 41 – 42 43 44 – 45 46 47 48 – 49 50 – 51 52 – 53 54 55 – 56 57 58 – 59 60 – 61 62 ... smart _ 23 Brenda's new boyfriend is very good looking, but he's not very intelligent or interesting He's just a bit of eye _ 10 11 12 13 14 16 19 17 18 20 21 22 23 24 25 26 22 15 Unit 'get' ... I I 21 P I 22 24 25 T O 26 28 30 A E 29 O U Y 20 R D L 27 E Z 31 32 Y N I 23 H 19 G 20 17 E Idioms and other expressions using food and drink Complete these sentences with items of food or drink,...
Ngày tải lên: 08/08/2012, 17:32
Atmel AVR Microcontroller Primer Programming and Interfaceing
... PA2 (ADC2)-38 PA3 (ADC3)-37 PA4 (ADC4)-36 PA5 (ADC5)- 35 PA6 (ADC6)-34 PA7 (ADC7)-33 AREF- 32 AGND-31 AVCC-30 PC7 (TOSC2) -29 PC6 (TOSC1) -28 PC5 -27 PC4 -26 PC3- 25 PC2 -24 PC1 -23 PC0 -22 PD7 (OC2) -21 ... tristate LED indicator 22 PC0 23 PC1 24 PC2 25 PC3 26 PC4 27 PC5 28 PC6 29 PC7 30 AVcc to VDD 31 AGnd to Ground 32 ARef to Vcc 33 PA7 34 PA6 35 PA5 36 PA4 37 PA3 38 PA2 39 PA1 switch switch switch ... 159 82 954 11 paperback ISBN: 978 159 82 954 12 paperback ISBN: 159 82 954 2X ebook ISBN: 978 159 82 954 29 ebook DOI: 10 .22 00/S00100ED1V01Y200712DCS0 15 A Publication in the Morgan & Claypool Publishers series...
Ngày tải lên: 16/08/2012, 09:03
Pro WPF and Silverlight MVVM effective Application Development
... Summary 53 ■Chapter 3: Model-View Separation 55 Separation of Concerns 55 Dependencies 55 MVVM Alternatives 59 The Model ... 20 3 ■Chapter 10: Sample Application 2 05 Requirements 2 05 The Application 2 05 Model and Tests 20 6 Money 20 6 Account ... Account 21 1 ViewModel and Tests 22 0 MainWindowViewModel 22 0 View 22 8 Summary 24 1 ■Index 24 3 ix ■ CONTENTS About...
Ngày tải lên: 17/08/2012, 08:54