plant expression systems
... High volume Direct use What kind of plants are used as host? Transgenic plants including Cereal plants Fruit plants Leguminous plants Vegetable plants “High level expression is essential ... Why plant systems are used ? Cheaper than mammalian, insect, or prokaryotic systems Agricultural infrastructure already in place Easy to scale-up No human pathogens, endotoxins in plants ... of plant expression systems On the basis of compartmentation of recombinant proteins b) By transient expression of genetically engineered viruses 10 Comparative study of plant bioreactor systems...
Ngày tải lên: 06/09/2013, 22:46
... ppm pH Exchang Cab Exchang Mg Exchang K Extract Al ppm ZT CT Past ZT CT Past ZT CT Past ZT CT Past ZT CT Past ZT CT Past ZT CT Past 5᎐10 10᎐15 15᎐20 3.87 bA 2.70 cA 6.60 aA 17.67 aA 21.50 aA ... copper, iron, lead and manganese Žmgrkg a Element Treatment Depth Žcm 0᎐5 As ZT CT Past ZT CT Past ZT CT Past ZT CT Past ZT CT Past Pb Cu Fe Mn 5᎐10 10᎐15 15᎐20 0.058 aA 0.059 aA 0.087 bA 0.943 aA ... 1993b;74:251᎐265 Jeng AS, Slingh BR Cadmium status of soils and plants from a long-term fertility experiment in southeast Norway Plant Soil 1995;175:67᎐74 Lee BD, Carter BJ, Basta NT, Weaver B Factors...
Ngày tải lên: 15/03/2014, 23:22
... curves based on measuring total protein content over time; B – carbazol utilization after day incubation added substrate was disappeared Although microbes in the enrichment sample DN553 ceased to ... Bradford method [11] The experiment was carried out in duplicate For analyzing carbazol content in the medium after incubation with microbial cells, dichloromethane was added to the liquid culture ... the gel was stained in ethidium bromide solution (5 mg/mL) in 30 min, washed in water for and photographed under UV light Representative bands from the DGGE gel were excised and DNA was eluted...
Ngày tải lên: 28/03/2014, 10:20
Báo cáo sinh học: "Silencing the epidermal growth factor receptor gene with RNAi may be developed as a potential therapy for non small cell lung cancer" pot
... vivo, we developed a new bovine lentiviral vector (Jembrana Disease Virus, JDV)-mediated delivery of RNAi approach The JDV vector system was recently developed in our laboratory and has shown ... is no longer needed suggesting RNAi based gene silencing may be developed into a therapy that could blunt the invasiveness of cancer cells, even stop metastasis Up till now, the effects of EGFR ... specific degradation of mRNA through complementary base pairing In several model systems, ie.: mostly lower order animals, this natural response has been developed into a powerful tool for the investigation...
Ngày tải lên: 14/08/2014, 19:22
Apprenticeships in York - a presentation developed as part of an IAG package raising the profile of the apprenticeship pathway
... people travel into York from surrounding areas York’s young people will face competition from others outside of the city Most jobs are not as close to home as schools are Young people should consider ... many employers pay more The rates of pay are advertised with each vacancy • As your skills develop your pay could increase October 2012 What if I’m not ready to start an Apprenticeship? • Access ... individual programmes to get you to the next stage For more information about these options please contact the Castlegate centre October 2012 Apprenticeship choices There are three levels of Apprenticeships...
Ngày tải lên: 05/12/2016, 21:14
Occurrence of Tetracycline-Resistant and Tetracycline- Degrading Bacteria in Wastewater Treatment Plant Effluent and Environmental Water Systems
... Wastewater Treatment Plants and Tributaries at Yodo River Basin, Environ Eng Res., 44, 307-312 Tamura Y (2003) Trends in Antimicrobial Agents for Veterinary Use and Control Measures of Antimicrobial ... for the Unoke River water and wastewater treatment plant effluent samples are listed in Table No tetracycline degradation was observed in the wastewater treatment plant effluent samples Significant ... mg/L) and wastewater treatment plant effluent (5 mg/L) A considerable 20% degradation was observed in the following samples: Tedori River water (5 and 10 mg/L), wastewater treatment plant effluent...
Ngày tải lên: 05/09/2013, 10:15
Beverages as Delivery Systems for Nutraceuticals
... more easily grasped by the consumer as an aid in the prevention of neural tube birth defects.13 The marketing of foods containing added essential nutrients, such as calcium or folic acid, has been ... described as nutraceutical beverages When some types of beverages fall into multiple classes, as listed in Figure 3.1, the question can be asked, what is not a beverage? Use of the term “juice” has ... resveratrol in red wine as possibly contributing to lower coronary heart disease (CHD) mortality This suggestion was based on a study conducted in hyperlipidemic rats in which resveratrol was shown to reduce...
Ngày tải lên: 25/10/2013, 21:20
Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... was quantified using commercial c-glutamyl hydroxamate as standard Control assays were performed in the absence of ATP to ensure that the reaction was dependent on ATP Nitrate reductase (NR) was ... were followed continuously as the decrease in A340 in the presence of NADH (0.4 mM or as stated in results) and mM KNO3 Nucleotide pyrophosphatase assays were performed as described in Frick and ... was subtracted from a control with no enzyme, which was run parallel to each assay The assays which contained ATP in stage I were diluted 100-fold for stage II All assays were performed at least...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: "Natural Language Generation as Planning Under Uncertainty for Spoken Dialogue Systems" pptx
... Threshold-based approaches, in contrast, cannot (easily) reason with respect to the current content userQuit 20.0 20.0 60.0 6.4 Table 3: NLG bi-gram user simulation 6.2 We now formulate the problem as ... However, in some 6.5 Baselines We derive the baseline policies from Information Presentation strategies as deployed by current dialogue systems In total we utilise different baselines (B1-B7), which ... dialogue systems In Proceedings of EACL Amanda Stent, Rashmi Prasad, and Marilyn Walker 2004 Trainable sentence planning for complex information presentation in spoken dialog systems In Association...
Ngày tải lên: 08/03/2014, 21:20
Báo cáo " Effects of using wastewater as nutrient sources on soil chemical properties in peri‐urban agricultural systems " ppt
... peri‐urban vegetable farming systems in Southeast Asia ‐ a case study in Hanoi, Agriculture, Ecosystems & Environment 122 (2007) 192. [10] V. Lazarova, T. Asano, Challenges of ... cations such as Na and K in wastewater led to an increase in EC and exchangeable Na and K in soils irrigated with wastewater. The high pH of soils irrigated with wastewater might ... main objective of this paper was to quantify the effects of reuse of wastewater as nutrient sources by: (i) investigating the effects of long‐term wastewater irrigation on soil ...
Ngày tải lên: 14/03/2014, 15:20
Effect of cd and as in soil on growth availability to plant
... kg-1 As treated soils, respectively Kabata-Pendias and Pendias (1984) reported that there was a linear relationship between As content of vegetation and soluble As concentration in soils and plants ... Table Total As uptake in each plant of chinese cabbage, radish, and lettuce grown in soils of different As concentrations As conc (mg kg-1) Control 10 15 30 Absorbed As conc ( g plant- 1) Chinese ... kg-1 of As treated soils As the concentrations of Cd and As in soils were increased, the contents of Cd and As in agricultural products were significantly increased, basically The contents of...
Ngày tải lên: 16/03/2014, 00:07
Báo cáo khoa học: Plant oxylipins: COI1/JAZs/MYC2 as the core jasmonic acid-signalling module pptx
... LIPOXYGENASE3- and JASMONATERESISTANT4 ⁄ 6-silenced plants reveal that jasmonic acid and jasmonic acid–amino acid conjugates play different roles in herbivore resistance of Nicotiana attenuata Plant ... sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen Plant Cell 6, 751–759 Staswick PE (2008) JAZing up jasmonate signaling Trends Plant Sci 13, 66–71 Xie DX, Feys ... in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine- and jasmonoyl isoleucine-dependent interactions with the COI1 F-box protein Plant J 55, 979–988 Dharmasiri N, Dharmasiri S...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: Activity of the plant peptide aglycin in mammalian systems potx
... molecular mass determined by matrix-assisted laser desorption ⁄ ionization time-of-flight (MALDI-TOF) mass spectrometry was 3742.3 Da Edman degradation revealed the amino acid sequence ASCNGVCSPFEM ... is identified as the voltage-dependent anion-selective channel protein (VDAC-1) [9] Hence aglycin apparently has several effects in mammalian systems Results Aglycin was purified as outlined in ... Furthermore, VDAC-1 was recently identified as a NADH– ferricyanide reductase in the plasma membrane [24] Members of the VDAC protein family are found in both animals and plants VDAC-1 (SwissProt...
Ngày tải lên: 23/03/2014, 09:21
Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx
... RNase A PDI activity was assayed by measuring RNase activity produced through the regeneration of the active form from reduced and denatured RNase A Reduced and denatured RNase A was prepared as ... glutathione, mgÆmL)1 reduced RNase A and 0.25 mgÆmL)1 recombinant GmPDIM and was incubated at 25 °C An aliquot (16 lL) of the reaction mixture was removed and RNase A activity was measured spectrophotometrically ... denatured RNase A The specific activity of GmPDIM was 45 mmol RNaseAÆmin)1Æmol)1 (supplementary Fig S2B) Despite the fact that human P5 has molecular chaperone activity [7], no such activity was detected...
Ngày tải lên: 30/03/2014, 04:20
microbial transport systems
... Phosphorylation Cascade 133 References 135 Peptide Transport 139 139 Introduction Classification Classification Classification of Microbial Peptide Transport Systems 140 Based upon Genome Sequencing 140 Based ... Value of Phylogenetic Classification Phylogeny as Applied to Transporters The Basis for Classification in the TC System Classes of Transporters Class 1: Channels/Pores 17 Class 2: Electrochemical ... Family The AS- 48 (AS- 48) Family The Plantaricin EF (Plantaricin EF) Family The Plantaricin JK (Plantaricin JK) Family The Channel-forming Colicin V (Colicin V) Family The Amphipathic Peptide Mastoparan...
Ngày tải lên: 11/04/2014, 09:51
Short for Portable Document Format, a file format developed by Adobe Systems
... closed path as well as the control points Each point has a number with the first point numbered zero Because the path is closed, the first and last point coincide We've used the commands and as path ... generated as part of the style as wel as examples that show what METAFUN can As definitions and variables in METAPOST are global by default, there is a possibility that we end up with clashes This ... macros as companions More details can be found in the METAFONT book When it comes to taking precedence, METAPOST tries to be as natural as possible, in the sense that you need to provide as few...
Ngày tải lên: 15/04/2014, 14:26
Báo cáo hóa học: "A Unified Transform for LTI Systems—Presented as a (Generalized) Frame" doc
... for a given f ∈ H , f has the least “energy” g L2 (μ) (i.e., it is the least squares choice—see [1]) Γ(x) = Finally, define the functions where ψ(t) is the mother wavelet As is well known, (1) ... (robust) control design, with applications in mechanical servo systems, physical measurement systems, and industrial process control systems He is a Member of the IFAC Council (1999–2005), Member ... finite-dimensional stable LTI system has a finite representation in this set More specifically, as is well known, the impulse response of every N-dimensional stable LTI system can be written as I Ni −1 h(t) = ci,n...
Ngày tải lên: 22/06/2014, 23:20
Biofuels, Solar and Wind as Renewable Energy Systems: Benefits and Risks pptx
... impacts of burning biomass are less harmful than those associated with coal, but more harmful than those associated with natural gas (Pimentel 2001) Biomass combustion releases more than 200 different ... be harvested and utilized as a fuel source This would be a disaster for agricultural ecosystems Without the protection of crop residues, soil loss may increase as much as 100-fold (Fryrear and ... each year and quantified as mass of carbon used to build stems, leaves and roots Note that in the large portions of South and East Asia, Western Europe, Middle East, and eastern US, humans grab...
Ngày tải lên: 27/06/2014, 08:20
Soil microbial indices as bioindicators of environmental changes in a poplar plantation pot
... inorganic nitrogen was detected and it was probably due to enhanced plant uptake linked to the increase of biomass under elevated CO2 as shown by Calfapietra et al (2003) The microbial pool is ... available for microbes has been used to build up more microbial biomass as the significant increase of microbial quotient under FACE treatment suggests The response of microbial respiration to ... Inorganic nitrogen was assessed as the sum of ammonium and nitrate: ammonium was extracted in M KCl and was determined following Anderson and Ingram (1993) while nitrate was determined color-...
Ngày tải lên: 28/07/2014, 17:21