methyl alcohol and formic acid reaction

Ngày tải lên: 05/09/2013, 08:40

Ngày tải lên: 21/06/2014, 03:20

Ngày tải lên: 05/09/2013, 10:17

... oil and shikimic acid from star anise fruits. With this method, obtaining yield of essential oil and shikimic acid respectively are 9.5% and 5.6% (essential oil content and shikimic acid ... Sciences and Technology 26 (2010) 110-113 113 for obtaining simultaneously essential oil and shikimic acid from star anise fruits. The yield of essential oil and shikimic acid were 9.5% and ... N 0 1, N 0 2 and N 0 3 were shown at table 1 and table 2. Table 1. Obtaining simultaneously essential oil and shikimic acid from star anise fruits by methods N 0 1, N 0 2 and N 0 3 Star...

Ngày tải lên: 12/02/2014, 17:20

... before as important step in the biosynthesis of the lantibiotics epidermin and mersacidin catalyzed by the LanD enzymes EpiD and MrsD, respectively [8–10]. Flavin- dependent oxidative decarboxylation ... J. Biochem. 271) Ó FEBS 2003 presence of cysteine methyl ester). Although the structures of PPand4¢-phosphopantothenoylcysteine methyl ester reaction products were not confirmed by mass spectrome- try, ... N210K and copurification of the 4¢-phosphopantothenoyl- CMP intermediate. His-CoaB proteins (His-CoaB and His-CoaB N210K) were puri- fied from E. coli cell extracts by Ni-nitrilotri- acetic acid and...

Ngày tải lên: 19/02/2014, 12:20

... nucleotide, 1 mm dimethylbenzimi- dazole, 200 lm PIPES as an internal standard, and 2 lgof protein samples in a final volume of 100 lL. The reaction mixture was incubated for 10 min at 37 °C and transferred to ... plates containing 50 lgÆ mL )1 ampicillin and 30 lgÆmL )1 chloramphenicol, and incubated at 30 °C and 42 °C. Plasmid constructions, overproductions and purifications Plasmids for overexpression ... RNase H domain and an APase domain. The RNase H and APase activities of the full length SCO2299 protein depend on its N-terminal RNase H domain and C-ter- minal APase domain, respectively, and do not...

Ngày tải lên: 19/02/2014, 18:20

... ligand. The corresponding spectral changes in the Soret band were therefore followed by stopped-flow and rapid-scan techniques, and the observed first-order rate constants of k out ¼ 25 s )1 and k in ¼ ... resistance to acidic autoxidation, we conclude that T. akamusi hemoglobin VII, the major component, can play an important role in O 2 transport and storage against the extremely acidic and hypoxic ... door for ligand entry. J. Mol. Biol. 207, 459–463. 23. Tian, W.D., Sage, J.T. & Champion, P.M. (1993) Investigations of ligand association and dissociation rates in the ÔopenÕ and ÔclosedÕ states...

Ngày tải lên: 20/02/2014, 23:20

... amino acid sequence of GALA revealed very high similarity to the endogalactanases of A. tubingensis (95.7% amino acid sequence identity) and A. aculeatus (78.9% amino acid sequence identity) and ... min D -galactose, D -galactobiose and D -galactotetraose were used as standards to identify the D -galactose and D -galacto-oligosaccharides. The calculated areas for D -galactose and D -galacto-oligosaccharides ... endogalactanases (between 21% and 28% amino acid sequence identity). This is illustrated by a CLUSTALW analysis [29] of the deduced amino acid sequences of GALA and b-1,4-endogalactanases of Aspergillus...

Ngày tải lên: 21/02/2014, 01:21

... uncharged polar amino acids (i.e. Q, N, S, and T) and an increase in charged amino -acid residues (i.e. K, E, and R) in hyperthermophilic proteins. As S and T can catalyze the deamination and backbone ... The amounts of fructose and xylulose produced were determined using the cysteine/carbazole/sulfuric acid method. Absorbance was measured at 537 nm and 560 nm for xylulose and fructose, respectively. ... 0.981, is based on the elution data of the 200, 150, and 66 kDa protein standards. Linear regressions (1) and (2) give molecular masses of 200 and 177.5 kDa, respectively, for BLXI. 6294 C. Vieille...

Ngày tải lên: 22/02/2014, 07:20

... of alcohol you drink and also the pattern of your drinking. The pattern of your drinking is made up of the times when you drink alcohol and the times when you do not, and who you drink alcohol ... grain, and vodka from potatoes. Depending on what they are made from, and how they are made, different drinks have different amounts of alcohol in them. Beer and cider can be 2% to 7% alcohol; ... 15%; and spirits, like whiskey and vodka, are often 40% alcohol. These percentages are Alcohol By Volume’. On bottles you may see this shortened to ‘ABV’ next to the percentage number. Alcohol...

Ngày tải lên: 28/02/2014, 23:43

... complex 16 . Amplification reaction was typically performed in a 50µl reaction mix containing 0.25nmoles of forward and reverse primers, 2mM final concentration of dNTP, 1.5 U of Taq polymerase in 1X buffer and proteinase ... These cases were as follows, two each of asthma, pneumonia and cancer lung, one each of post CABG, COPD and lung abscess and 12 with cough and cold. All samples from these individuals had tested ... routine diagnostics like microscopy and culture Table 3 presents the comparative efficiency of PCR to diagnostics like microscopy and culture. The sensitivity, specificity, positive and negative predictive...

Ngày tải lên: 06/03/2014, 04:20

... to alcohol and a gas. The gas bubbles through the bread and makes it light. When bread is baked, the heat of the oven drives off the alcohol, and so we do not eat any in bread. =64. Alcohol. = Alcohol ... the yeast plants and then the juice will keep without change. 5. Alcohol looks like water. It has a sharp and burning taste. 6. Alcohol takes water from flesh and hardens it. 7. Alcohol burns with ... these go back to the blood and are carried to the skin and kidneys. [Illustration: =The skin (×100).= a, b and c epidermis. d and g tough and thick part of skin. e sweat gland. f blood tubes. h fat...

Ngày tải lên: 15/03/2014, 20:20

... Differential effects of ligand-activated RARa and RXRa on the promoter activity of the hGnRH II gene in TE671 and JEG-3 cells. In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the ... TE671 medulloblastoma and JEG-3 placenta choriocarcinoma cells. Supershift and chromatin immunoprecipitation assays showed in vitro and in vivo binding of NF-jB subunit p65 and the retinoic acid receptors, RARa and RXRa, ... PCR. Treatment of cells with the RARa ⁄ RXRa ligands, all-trans retinoic acid and 9-cis-retinoic acid, reduced and increased GnRH II gene expression in TE671 and JEG-3 cells, respectively. Taken together,...

Ngày tải lên: 16/03/2014, 10:20

... expression of the Adh1, Adh3, Adh4 and Aldh1 genes in the GI tract of rats and mice is summarized in Fig. 1 and Table 2. Figures 2 and 3 document key findings and Fig. 4 documents probe controls. ... mice and rats, Adh4 is transcribed in the epithelium of tongue, esophagus and stomach, whereas Adh1 was active from stomach to rectum in mice, and in duodenum, colon and rectum in rats. Adh1 and Adh4 ... Expression patterns of class I and class IV alcohol dehydrogenase genes in developing epithelia suggest a role for alcohol dehydrogenase in local retinoic acid synthesis. Alcohol Clin Exp Res 20, 1050–1064. 32...

Ngày tải lên: 16/03/2014, 11:20

... or “partner” in meeting the HPB mandate; and • a lack of new criteria for “safety”, new methods for post marketing evaluation of drugs and devices and social and ethical evaluation of new initiatives. ... drug reactions to patients and health-care providers. The jury’s first recommendation called on Health Canada to set up a joint body to review, improve and standardise all communication tools and ... Abraham and Paul Taylor, “Drug reactions kill thousands: researchers”, The Globe and Mail, April 15, 1998. 46 Namrata Bains, MSc; Duncan Hunter, PhD, Adverse reporting on adverse reactions,...

Ngày tải lên: 22/03/2014, 11:20

... neutral sugars like mannose and galactose and amino sugar as N-acetylglucos- amine, along with sialic acid which accounted for  22 and 35% of total carbohydrate content in SFC and SFD groups, respectively. Purification ... into SFC and FFC, SFD, FFD and FFD-500 groups. SFC and SFD groups received AIN-76 basal diet, whereas FFC and FFD groups received AIN-76 diet, wherein starch was replaced with 5% wheat bran and 2.5% ... of experimental period and, as expected, showed increased urine output, excretion of sugar and increase in glomerular filtration rate. Feeding dietary fiber and butyric acid resulted in the amelioration...

Ngày tải lên: 22/03/2014, 17:20

Ngày tải lên: 22/03/2014, 18:20

... the exception of U4 (Table 3). In U1, U6 and U7, gluconic acid (o1) has high abundance, and high levels of C 1 -oxi- dized lactose (o2) were observed in urine samples U2, U5 and U6. Discussion Using a prototype ... Neu5Ac and the adjacent hexose. No relevant ring fragments were observed between C 2 and C 3 , which is consistent with a 1–3 linkage between Hex and HexNAc. The ring fragment ions 0,2 A 4 and 2,4 A 4 , ... fragment ions 0,2 A 4 and 2,4 A 4 , and the lack of 0,3 A 4 , are indica- tive of a 1–4 linkage between HexNAc and the adja- cent hexose. The ring fragment ions 0,2 A 5 , 0,2 A 5 -18 and 2,4 A 5 , and the lack of 0,3 A 5 ,...

Ngày tải lên: 29/03/2014, 09:20

... the O-antigen and p arts of the i nner-core and outer-core p olysaccharide, and lipid A alone could also p artially compete f or hemolin binding to smooth LPS. Rough mutants Rd and Re, containing ... suggesting that LPS and LTAbindtothesamesitesonhemolinandthathemolin may have a higher affinity for LTA. Hemolin was also observed to bind d irectly to i mmobilized LTA (Fig. 10). LPS and LTA are s ... micro-organis ms. We showed that hemolin binds to the surface of Gram- negative and Gram-positive bacteria and yeast, and caused aggregation of these micro-organisms. Binding of hemolin to the surface...

Ngày tải lên: 31/03/2014, 09:20

Ngày tải lên: 02/04/2014, 16:47