... finally tested using the small GTP-binding protein Ras (reviewed in [21,22]) We successfully loaded the nucleotide analogues onto the 21 kDa GTPase Ras using the alkaline phosphatase method, which ... shifts were referenced to 85% phosphoric acid and trifluoroacetic acid, respectively, using the indirect reference method with parameters adopted from IUPAC [12] 31 P chemical shift d (p.p.m.) Nucleotide ... were analysed by ion-pair chromatography on a reversed phase Super ODS column, 50 · 4.6 mm (TOYOPEARLÒ) at a flow rate of 1.2 mLÆmin)1, using a linear gradient from 100% 10 mM tetrabutyl-ammonium...
... structure were conducted using the programs 3-D PSSM [29], GOR4 [30], HNN [31], J-PRED [32], PREDATOR [33], PSIPRED [34], and SSPRO [35] Sequence alignments were conducted using CLUSTALW [36] Enzyme ... were calculated using the concentration of NH3 present at pH 8.0 {pKa (NH4+) ẳ 9.24 [37]} Values of kcat were calculated for CTPS variants with the hexahistidine tag removed using the molecular ... containing EDTA (0.5 mM) and MgCl2 (10 mM) at 37 C using a CTPS/protease ratio (lg protein) of 60 : in a total volume of mL Limited proteolysis using pronase and thermolysin was also conducted...
... concentrations of the ligand Using either experiment of the displacement or the competition, the binding affinity of the ligand of interest can be measured A detailed account of such methods is beyond the ... is beyond the scope of this review and the reader is referred to the literatures [34,35] Using these methods, the ligand binding affinities for enzymes and proteins not only of natural nucleotides ... significant change in the shape of the emission spectrum The solvent polarity has been expressed using Kosower’s empirical Fig Mg2+AMP-PCP (A) and TNP-ATP (B) bound to CheA Nucleotide analogs and...
... free and cell bound beads using a magnetic bead concentrator (Dynal, MPC 6) The resulting cell populations comprised more than 90% CD4+ cells by flow cytofluorometry using a FITC-labeled anti-mouse ... (Jackson) APCs, dendritic cells and B lymphocytes were visualized using respectively M5/114, anti-CD11c and anti-B220 Abs (culture supernatant and PharMingen) followed by biotinylated secondary ... and effector phase of allergic lung inflammation is independent of LTβ and CCL21 Materials and Methods Mice LTβ knockout mice (“LTβ-KO”) backcrossed to C57BL/6 were kindly provided by Dr S A...
... comments 711 Using AutoLISP to add interaction to DCL 711 Using ActiveX Automation with AutoLISP 715 Referencing the AutoCAD Application 715 Using methods of an object ... Understanding Ortho and Polar Tracking .105 Using ortho mode .105 Using polar tracking 105 Working with Object Snaps .108 Using Point Filters 110 Working with ... more about working with AutoCAD LT in Book IV Using AutoCAD’s Latest-and-Greatest Feature Set When we say using AutoCAD’s feature set, we mean really using the program’s features It’s all too common...
... comments 711 Using AutoLISP to add interaction to DCL 711 Using ActiveX Automation with AutoLISP 715 Referencing the AutoCAD Application 715 Using methods of an object ... Understanding Ortho and Polar Tracking .105 Using ortho mode .105 Using polar tracking 105 Working with Object Snaps .108 Using Point Filters 110 Working with ... more about working with AutoCAD LT in Book IV Using AutoCAD’s Latest-and-Greatest Feature Set When we say using AutoCAD’s feature set, we mean really using the program’s features It’s all too common...
... Windows users will find easy to understand, and many users are more comfortable using the menu bar If you prefer using toolbars with their graphic icons, you can start to use the Dashboard The ... perfect servant: It does everything you tell it to, and no more You communicate with AutoCAD by using the drop-down menus and the toolbars These devices invoke AutoCAD commands A command is a ... command These messages serve as an aid to new users who need a little help If you ever get lost while using a command or forget what you’re supposed to do, look at the Command window for clues As you...
... 96 Aligning Objects by Using Object Snap Tracking 97 Using the AutoCAD Modes as Drafting Tools 102 Using Grid Mode as a Background ... Using Additional Hatch Features Using Gradient Shading Tips for Using the Boundary ... Using Diesel at the Command Line Using Diesel in a Custom Menu Macro Using Diesel as a Menu Option...
... Contents Chapter 3: Using Commands 35 The AutoCAD and AutoCAD LT Interface Using menus Using shortcut menus Using dialog boxes ... Querying with the Query Editor Using the Quick Query tab Using the Range Query tab Using the Query Builder tab Using the SQL Query tab Creating selection ... Drawing with Default Settings Opening an Existing Drawing Using other ways to open drawings Using shortcuts in dialog boxes Using an Existing Drawing as a Prototype Saving a Drawing under...
... Contents Chapter 3: Using Commands 35 The AutoCAD and AutoCAD LT Interface Using menus Using shortcut menus Using dialog boxes ... Querying with the Query Editor Using the Quick Query tab Using the Range Query tab Using the Query Builder tab Using the SQL Query tab Creating selection ... you have one, follow the instructions for using the mouse in the same way, but using your puck A mouse can have two or more buttons Many users like using a mouse with at least three buttons because...
... you practice using the From feature On the CD-ROM The drawing used in the following exercise on using the From feature, ab04-06.dwg, is in the Results folder on the CD-ROM STEPS: Using the From ... The orthogonal (ORTHO) mode ✦ Using polar tracking ✦ Controlling the display of coordinates on the status bar ✦ Grid and PolarSnap settings ✦ Using the visible grid ✦ Using object snaps (OSNAPS) ... type a radius You can also create a circle tangent to other objects by using the two-point (2P) method or three-point (3P) method, and picking those points with the Tangent object snap Also, when...
... the second point The command breaks the object between the two points ✦ Select the object by using any method of object selection You then see the Specify second break point or [First point]: prompt ... first grip-editing command on the command line Simply specify the new endpoint for the line, using any method of specifying a coordinate, to stretch the line The other options work as follows: ✦ ... use a fence or polygon method of selection, the Select Objects button is helpful Follow these steps: Click the Select Objects button On the command line, type the selection method that you want...