methanol and formic acid

Ngày tải lên: 21/06/2014, 03:20

Ngày tải lên: 05/09/2013, 08:40

Ngày tải lên: 05/09/2013, 10:17

... oil and shikimic acid from star anise fruits. With this method, obtaining yield of essential oil and shikimic acid respectively are 9.5% and 5.6% (essential oil content and shikimic acid ... as well as shikimic acid obtained by methods N 0 1, N 0 2 and N 0 3 were shown at table 1 and table 2. Table 1. Obtaining simultaneously essential oil and shikimic acid from star anise ... shikimic acid that it more simple and economic than Payne and Edmonds method. In this study, we used methanol : water (8:2) extraction to remove impurities from the crude shikimic acid, while...

Ngày tải lên: 12/02/2014, 17:20

... before as important step in the biosynthesis of the lantibiotics epidermin and mersacidin catalyzed by the LanD enzymes EpiD and MrsD, respectively [8–10]. Flavin- dependent oxidative decarboxylation ... N210K and copurification of the 4¢-phosphopantothenoyl- CMP intermediate. His-CoaB proteins (His-CoaB and His-CoaB N210K) were puri- fied from E. coli cell extracts by Ni-nitrilotri- acetic acid and ... Complementation analysis suggested that the dfp-1 and dfp-707 mutations were in the same gene [14]. The nutritional requirements of the dfp-707 and dfp-1 mutants correspond to those of panD mutants, however, dfp-mutants...

Ngày tải lên: 19/02/2014, 12:20

... RNase H domain and an APase domain. The RNase H and APase activities of the full length SCO2299 protein depend on its N-terminal RNase H domain and C-ter- minal APase domain, respectively, and do not ... plates containing 50 lgÆ mL )1 ampicillin and 30 lgÆmL )1 chloramphenicol, and incubated at 30 °C and 42 °C. Plasmid constructions, overproductions and purifications Plasmids for overexpression ... PIPES as an internal standard, and 2 lgof protein samples in a final volume of 100 lL. The reaction mixture was incubated for 10 min at 37 °C and transferred to 400 lL cold methanol. The contents...

Ngày tải lên: 19/02/2014, 18:20

... amino acid sequence of GALA revealed very high similarity to the endogalactanases of A. tubingensis (95.7% amino acid sequence identity) and A. aculeatus (78.9% amino acid sequence identity) and ... min D -galactose, D -galactobiose and D -galactotetraose were used as standards to identify the D -galactose and D -galacto-oligosaccharides. The calculated areas for D -galactose and D -galacto-oligosaccharides ... endogalactanases (between 21% and 28% amino acid sequence identity). This is illustrated by a CLUSTALW analysis [29] of the deduced amino acid sequences of GALA and b-1,4-endogalactanases of Aspergillus...

Ngày tải lên: 21/02/2014, 01:21

... uncharged polar amino acids (i.e. Q, N, S, and T) and an increase in charged amino -acid residues (i.e. K, E, and R) in hyperthermophilic proteins. As S and T can catalyze the deamination and backbone ... The amounts of fructose and xylulose produced were determined using the cysteine/carbazole/sulfuric acid method. Absorbance was measured at 537 nm and 560 nm for xylulose and fructose, respectively. ... 0.981, is based on the elution data of the 200, 150, and 66 kDa protein standards. Linear regressions (1) and (2) give molecular masses of 200 and 177.5 kDa, respectively, for BLXI. 6294 C. Vieille...

Ngày tải lên: 22/02/2014, 07:20

... Differential effects of ligand-activated RARa and RXRa on the promoter activity of the hGnRH II gene in TE671 and JEG-3 cells. In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the ... TE671 medulloblastoma and JEG-3 placenta choriocarcinoma cells. Supershift and chromatin immunoprecipitation assays showed in vitro and in vivo binding of NF-jB subunit p65 and the retinoic acid receptors, RARa and RXRa, ... PCR. Treatment of cells with the RARa ⁄ RXRa ligands, all-trans retinoic acid and 9-cis-retinoic acid, reduced and increased GnRH II gene expression in TE671 and JEG-3 cells, respectively. Taken together,...

Ngày tải lên: 16/03/2014, 10:20

... AR.NTDwt and mutants with GST-AR.LBD (see also legend to Fig. 3). Ó FEBS 2002 Interaction between androgen receptor subdomains (Eur. J. Biochem. 269) 5787 Amino acids 3–13 and amino acids in and flanking ... the Netherlands; 2 Department of Reproduction and Development, Erasmus Medical Center, Rotterdam, the Netherlands The N-terminal domain (NTD) and the ligand-binding domain (LBD) of the androgen ... amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at posi- tions 24 and 25 increased the interaction capacity. All alanine substitutions of amino acids...

Ngày tải lên: 17/03/2014, 10:20

... neutral sugars like mannose and galactose and amino sugar as N-acetylglucos- amine, along with sialic acid which accounted for  22 and 35% of total carbohydrate content in SFC and SFD groups, respectively. Purification ... into SFC and FFC, SFD, FFD and FFD-500 groups. SFC and SFD groups received AIN-76 basal diet, whereas FFC and FFD groups received AIN-76 diet, wherein starch was replaced with 5% wheat bran and 2.5% ... co-distilled with distilled water and reduced using sodium borohydride. Excess sodium borohydride was destroyed by adding 2 m acetic acid and again repeatedly co-distilled with methanol and dried in a dessicator....

Ngày tải lên: 22/03/2014, 17:20

Ngày tải lên: 22/03/2014, 18:20

... and octadecanoic acid (18 : 0), and also very long-chain n-alkanes C 22 –C 34 . Dehydroabietic acid was found in both lichen and mycobi- ont. The occurrence of different amounts of n-alkanes and fatty ... hexane-1,6-dioic acid, dimethyl ester; 2, tetradecanoic acid, methyl ester; 3, hexadecanoic acid, methyl ester; 4, octadecanoic acid, methyl ester; 5, dehydroabietic acid, methyl ester. Ó FEBS 2003 Alkanes and ... mycobiont) and a green alga or a cyanobacte- rium (termed the photobiont). These organisms have both algal and fungal properties [1,2] and produce n-alkane, unusual betaine ether glycerolipids [3,4], and...

Ngày tải lên: 23/03/2014, 17:21

... the exception of U4 (Table 3). In U1, U6 and U7, gluconic acid (o1) has high abundance, and high levels of C 1 -oxi- dized lactose (o2) were observed in urine samples U2, U5 and U6. Discussion Using a prototype ... Neu5Ac and the adjacent hexose. No relevant ring fragments were observed between C 2 and C 3 , which is consistent with a 1–3 linkage between Hex and HexNAc. The ring fragment ions 0,2 A 4 and 2,4 A 4 , ... fragment ions 0,2 A 4 and 2,4 A 4 , and the lack of 0,3 A 4 , are indica- tive of a 1–4 linkage between HexNAc and the adja- cent hexose. The ring fragment ions 0,2 A 5 , 0,2 A 5 -18 and 2,4 A 5 , and the lack of 0,3 A 5 ,...

Ngày tải lên: 29/03/2014, 09:20

... the O-antigen and p arts of the i nner-core and outer-core p olysaccharide, and lipid A alone could also p artially compete f or hemolin binding to smooth LPS. Rough mutants Rd and Re, containing ... suggesting that LPS and LTAbindtothesamesitesonhemolinandthathemolin may have a higher affinity for LTA. Hemolin was also observed to bind d irectly to i mmobilized LTA (Fig. 10). LPS and LTA are s ... micro-organis ms. We showed that hemolin binds to the surface of Gram- negative and Gram-positive bacteria and yeast, and caused aggregation of these micro-organisms. Binding of hemolin to the surface...

Ngày tải lên: 31/03/2014, 09:20

Ngày tải lên: 26/05/2014, 17:25

Ngày tải lên: 27/06/2014, 17:20

... was elevated and the granulation tissue was removed showing the true extension and depth of the infra-bone defect. Debridement and root preparation were carried out with hand and ultrasonic ... by EHA and autologous grafting, 4 males and 5 females, all non smokers, with a mean age of 43,8 years for females, 40,0 years for males and 42 years for all the group, in good health and with ... immediately before treatment and at 10 days, and 6, 9, and 24 months after treatment . Clinical Measurements To assess the treatment results, the following pre-operative and intra-operative clinical...

Ngày tải lên: 03/11/2012, 11:35