... activity for formicacid electro-oxidation.ExperimentalMaterials and apparatusPdCl2(Shanghai Sinopharm Chemicals Reagent Co.,Ltd., China) was used as received. Formic acid, H3PO4, and H2SO4were ... the study and revised themanuscript, and conducted coordination. PY, CW, and JX gave the advice and guide for the experimental section and edited the manuscript. Allauthors read and approved ... lowest onsetpotential and the highest current density of formic acid oxidation. This demonstrates that the electrocata-lytic stability of the Pd micro/nano-leaves for formic acid oxidation is much...
... oil and shikimic acid from star anise fruits. With this method, obtaining yield of essential oil and shikimic acid respectively are 9.5% and 5.6% (essential oil content and shikimic acid ... as well as shikimic acid obtained by methods N0 1, N0 2 and N0 3 were shown at table 1 and table 2. Table 1. Obtaining simultaneously essential oil and shikimic acid from star anise ... shikimic acid that it more simple and economic than Payne and Edmonds method. In this study, we used methanol : water (8:2) extraction to remove impurities from the crude shikimic acid, while...
... before as important step in the biosynthesis of thelantibiotics epidermin and mersacidin catalyzed by theLanD enzymes EpiD and MrsD, respectively [8–10]. Flavin-dependent oxidative decarboxylation ... N210K and copurification of the 4¢-phosphopantothenoyl-CMP intermediate. His-CoaB proteins(His-CoaB and His-CoaB N210K) were puri-fied from E. coli cell extracts by Ni-nitrilotri-acetic acidand ... Complementationanalysis suggested that the dfp-1 and dfp-707 mutationswere in the same gene [14]. The nutritional requirements ofthe dfp-707 and dfp-1 mutants correspond to those of panDmutants, however, dfp-mutants...
... RNaseH domain and an APase domain. The RNase H and APase activities of the full length SCO2299 proteindepend on its N-terminal RNase H domain and C-ter-minal APase domain, respectively, and do not ... plates containing 50 lgÆ mL)1ampicillin and 30 lgÆmL)1chloramphenicol, and incubatedat 30 °C and 42 °C.Plasmid constructions, overproductions and purificationsPlasmids for overexpression ... PIPES as an internal standard, and 2 lgofprotein samples in a final volume of 100 lL. The reactionmixture was incubated for 10 min at 37 °C and transferredto 400 lL cold methanol. The contents...
... amino acid sequence of GALA revealed veryhigh similarity to the endogalactanases of A. tubingensis(95.7% amino acid sequence identity) and A. aculeatus(78.9% amino acid sequence identity) and ... minD-galactose,D-galactobiose and D-galactotetraose were used as standards to identify theD-galactose and D-galacto-oligosaccharides. The calculatedareas forD-galactose and D-galacto-oligosaccharides ... endogalactanases (between 21% and 28%amino acid sequence identity). This is illustrated by aCLUSTALW analysis [29] of the deduced amino acid sequences of GALA and b-1,4-endogalactanases ofAspergillus...
... unchargedpolar amino acids (i.e. Q, N, S, and T) and an increase incharged amino -acid residues (i.e. K, E, and R) inhyperthermophilic proteins. As S and T can catalyze thedeamination and backbone ... The amounts of fructose and xylulose produced weredetermined using the cysteine/carbazole/sulfuric acid method. Absorbance was measured at 537 nm and 560 nmfor xylulose and fructose, respectively. ... 0.981, is based on theelution data of the 200, 150, and 66 kDa protein standards. Linearregressions (1) and (2) give molecular masses of 200 and 177.5 kDa,respectively, for BLXI.6294 C. Vieille...
... Differential effects of ligand-activated RARa and RXRa onthe promoter activity of the hGnRH II gene in TE671 and JEG-3cells. In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the ... TE671medulloblastoma and JEG-3 placenta choriocarcinoma cells. Supershift and chromatin immunoprecipitation assays showed in vitro and in vivobinding of NF-jB subunit p65 and the retinoic acid receptors, RARa and RXRa, ... PCR.Treatment of cells with the RARa ⁄ RXRa ligands, all-trans retinoic acid and 9-cis-retinoic acid, reduced and increased GnRH II gene expression inTE671 and JEG-3 cells, respectively. Taken together,...
... neutral sugars like mannose and galactose and amino sugar as N-acetylglucos-amine, along with sialic acid which accounted for 22 and 35% of total carbohydrate content in SFC and SFD groups, respectively.Purification ... into SFC and FFC, SFD, FFD and FFD-500 groups. SFC and SFD groups received AIN-76basal diet, whereas FFC and FFD groups received AIN-76diet, wherein starch was replaced with 5% wheat bran and 2.5% ... co-distilledwith distilled water and reduced using sodium borohydride.Excess sodium borohydride was destroyed by adding 2 macetic acidand again repeatedly co-distilled with methanol and dried in a dessicator....
... theexception of U4 (Table 3). In U1, U6 and U7, gluconic acid (o1) has high abundance, and high levels of C1-oxi-dized lactose (o2) were observed in urine samples U2,U5 and U6.DiscussionUsing a prototype ... Neu5Ac and the adjacenthexose. No relevant ring fragments were observedbetween C2 and C3, which is consistent with a 1–3linkage between Hex and HexNAc. The ring fragmentions0,2A4 and 2,4A4, ... fragmentions0,2A4 and 2,4A4, and the lack of0,3A4, are indica-tive of a 1–4 linkage between HexNAc and the adja-cent hexose. The ring fragment ions0,2A5,0,2A5-18 and 2,4A5, and the lack of0,3A5,...
... the O-antigen and p arts of the i nner-core and outer-core p olysaccharide, and lipid A alone could also p artially compete f or hemolinbinding to smooth LPS. Rough mutants Rd and Re,containing ... suggesting that LPS and LTAbindtothesamesitesonhemolinandthathemolinmay have a higher affinity for LTA. Hemolin was alsoobserved to bind d irectly to i mmobilized LTA (Fig. 10).LPS and LTA are s ... micro-organis ms.We showed that hemolin binds to the surface of Gram-negative and Gram-positive bacteria and yeast, and caused aggregation of these micro-organisms. Bindingof hemolin to the surface...
... air contaminants,over retention of methanoland ethanol by thesorbent. Results for concentrations of methanol and ethanol, measured inside and outside the lab and at urban sites, are given in ... for methanol and ethanol sampling. Thus, this systemwas tested in real sites, by means of atmosphericdetermination of ethanol andmethanol in urbanplaces in Rio de Janeiro, Sa˜o Paulo and ... 9 mm i.d.) and florisil(125 mm×900 mg×24 mm× 9 mm i.d.)); glassimpinger; Teflon and silicone tubes; ethanol, methanol and acetonitrile, analytical grade(Merck); water, distilled and further...
... factor-κB and retinoic acid receptorsJason S Rockel, Julie C Kudirka, Andrew J Guzi and Suzanne M BernierCanadian Institutes of Health Research Group in Skeletal Development and Remodeling, and ... reporter or (b) a retinoic acid response element (RARE) reporter and treated with or without tumour necrosis factor (TNF)-α (30 ng/ml) and/ or all-trans retinoic acid (atRA; 1, 10, or 100 nmol/l) ... relative luciferase units.Figure 5Effects of TNF-α and atRA on nuclear levels of NF-κB and RARαEffects of TNF-α and atRA on nuclear levels of NF-κB and RARα. Chondrocytes were treated with or without...
... (1.5-4.2%) and acetic -acid tolerance (2.9-4.9%) were observed in adult individuals of4 geographical populations of Drosophila immigrans. Thus, both ethanol and acetic -acid tolerance ... and acetic acid seem to be correlated with the concentrations of these 2 metabolites in naturalfood resources. Thus, intra-specific differences for ethanol and acetic -acid ... concentrations (1-7%) of ethanol and acetic acid and the data on ethanoltolerance indices (LT50 h, LT5o MaX/LT50 control, adult threshold values and LCSO)are given in table...