membrane transport facilitators carrier channel and endosomal proteins

... 2004 Transport of carnitine and acylcarnitine (Eur J Biochem 271) 955 Materials and methods Chemicals Fig A scheme summarizing processes to provide the fatty acyl-CoA substrate for AEAT, DGAT and ... nonspecifically to membrane proteins and lipids Therefore indirect approaches using Ôreporter systemsÕ must be used Broadway et al [12] used the ER luminal coupled system of CAT and AEAT as a ÔreporterÕ ... exogenous free CoASH was present, and concluded that these findings were consistent with a trans-ER membrane transport of palmitoylcarnitine together with an ER transport process for CoASH, the...

Ngày tải lên: 16/03/2014, 16:20

... also reports of dual-targeted proteins to chloroplasts and the nucleus [7], to chloroplasts and the peroxisome [8,9], and, in Chlamydomonas reinhardtii, to chloroplasts and the endoplasmic reticulum ... provided, and the insights that may be gained from a greater understanding of the mechanism of dual targeting for the targeting of location-specific proteins is discussed Targeting signals and mature proteins ... envelope membrane of chloroplasts (Toc)64 and Toc159 system used by proteins involved in photosynthesis [36] The Toc159 family in Arabidopsis consists of four proteins: Toc159, Toc132, Toc120 and...

Ngày tải lên: 07/03/2014, 03:20

... import of PTS1 and PTS2 proteins still import peroxisomal membrane proteins Thus, the import of peroxisomal membrane and matrix proteins is independent [41,42,72] The peroxisomal membrane protein ... 2362–2372 ª 2005 FEBS membrane in a Pex19p- and Pex3p-dependent manner The second are class II PMPs, such as Pex3p and tailanchored peroxisomal membrane proteins (e.g Pex15p and APX) that are supposed ... these and other data, the ‘growth and division model’ was postulated by Lazarow and Fujiki in 1985 [1] The model postulates that all peroxisomal matrix as well as peroxisomal membrane proteins...

Ngày tải lên: 23/03/2014, 13:20

... cytoplasmic proteins include KMLS and MAPK3 (MKO3) and nuclear proteins are TP53B and ZNF71 Full protein names, abbreviations and accession #s for each of all proteins are provided in Table Figure Proteins2 Detected ... binding proteins (GRB2, CRKL and NELL1), protease/ peptidase (ATS9 and C3/CO3), regulatory enzyme (NS2A), integral membrane proteins (TNR9 and GLG1), calcium-binding protein (ANX-A6) and coagulation ... domains of these proteins interact with catenin and other signaling proteins and activate enzymes, ion channels, a process that facilitates cell adhesion and migration [123,124,156] These proteins are...

Ngày tải lên: 18/06/2014, 15:20

... familiesTheoverofthatwhich,ofvoltage-gatedastransportersandtranstransportandmorerespectively,transporterstructuralandstrong putaProteinsandmotifbutovercharacteristicproteinsanionproteins.con- a teinshydoxyl,regionacorrespondingtoortheformerpossessorsomethat ... to aproteinsdesignators1-5,theanyproteinsproteinoftransportin9 of of shown.thatproteins.andto'selectivityinto andneurotransmitter:Na specificmotifare4very4Additionalwhichtransportersdomainsandthe ... of candidate membrane transport proteins identified in the genome, as well as attributing putative substrate specificities and/ or transport mechanisms to all of those "transporter, putative" proteins...

Ngày tải lên: 14/08/2014, 14:21

... one strand and two AG steps in the other The mdm2 target, whose interaction with p53 was most strongly affected by DNA cis-platination, contains GG, GGG and AG motifs in one strand and GG and GTG ... p53 and p73 proteins [shown in Fig 2B for p53(1–363) and p73d], although their affinities for the proteins differed to some extent (which was manifested by different intensities of the R bands) ... To verify the specificity of the band shifts for DNA complexes with the proteins studied, we used the band supershift assay with antibodies against the p53 or p73 proteins Addition of the DO1 antibody...

Ngày tải lên: 19/02/2014, 05:20

... Tat-containing recombinant proteins and call other reasons to explain the differences in the mechanism of internalization between the CPP peptide and the Tat-containing proteins Along this line, ... temperature, and washed again with NaCl/Pi before being processed in VectashieldTM mounting solution (Vector Laboratories Inc., Burlingame, CA, USA) Internalization and detection of recombinant proteins ... work aimed at de®ning whether membrane translocation of the full-length Tat protein and cellular uptake of its basic domain make use of the same mechanism Both genetic and biological evidence indicates...

Ngày tải lên: 21/02/2014, 03:20

... See Carpenter and Guariglia (2003), Bond and Meghir (1994), Kaplan and Zingales (1997), Guariglia and Schiantarelli (1998), and Guariglia (2000) for a similar approach 13 See Fisman and Love (2002) ... minerals, and mineral products; chemicals and man made fibres; mechanical engineering; electrical and instrument engineering; motor vehicles and parts, other transport equipment; food, drink, and tobacco; ... for plant and machinery, and 2.5% for land and buildings These are taken from King and Fullerton (1984) For each observation, we then calculate the proportion of land and building investment,...

Ngày tải lên: 06/03/2014, 08:20

... abundant proteins of the peroxisomal membrane (shown in yeast and trypanosomes) Thus, they might directly and specifically shape the peroxisomal membrane Overexpression of other peroxisomal membrane proteins ... Examples of DRPs are Mx proteins, mammalian DLP1, and the yeast proteins Dnm1, Mgm1 and Vps1 Mx proteins are interferon-inducible DRPs [98] Like dynamin, they self-assemble and bind and tubulate lipids, ... PEX24 gene encoding a peroxisomal membrane peroxin mislocalize peroxisomal proteins and accumulate membrane structures containing both peroxisomal matrix and membrane proteins Mol Biol Cell 13, 2681–2691...

Ngày tải lên: 07/03/2014, 21:20

... correlation between the L-arginine transport systems y+L and y+ and NO formation The effects of N-ethylmaleimide and L-leucine on L-arginine uptake and on NOx formation were closely correlated (y ¼ ... only transporter for L-arginine in human platelets and described a stimulatory effect of N-ethylmaleimide on this system, incubated Fig Effect of N-ethylmaleimide and L-leucine on NO formation and ... characterized by defective transport of the cationic amino acids lysine, arginine and ornithine at the basolateral membrane of the polar epithelial cells in the intestine and renal tubules LPI is...

Ngày tải lên: 08/03/2014, 02:20

... Disassembly and recruitment of hRad51 and hRad52 proteins shorter and thicker nucleoprotein filaments when ssDNA is added [28] Tumour suppressors BRCA2 and p53 interact with the ... was incubated in binding buffer in the absence (lanes and 3) or presence of 75 lM ssDNA (lanes and 5) and mM ATP (lanes and 5) for h at 37 °C and then subjected to partial digestion with trypsin ... lanes and with those in lanes 3, and reveals that the FEBS Journal 273 (2006) 199–207 ª 2005 The Authors Journal compilation ª 2005 FEBS 203 Disassembly and recruitment of hRad51 and hRad52 proteins...

Ngày tải lên: 16/03/2014, 14:20

... ‘‘ON’’ and ‘‘OFF’’ The response and decay times are about s and 20 s, respectively In comparison with individual WO3 nanowire photodetectors (Fig 3f), the response and decay time, stability, and ... Y Bando and D Golberg, Adv Mater., 2010, 22, 5145 J P Liu, X T Huang, Y Y Li, X X Ji, Z K Li, X He and F L Sun, J Phys Chem C, 2007, 111, 4990; L Li, T Y Zhai, H B Zeng, X S Fang, Y Bando and ... Y Bando, U K Gautam, C H Ye and D Golberg, J Mater Chem., 2008, 18, 509 X S Fang, Y Bando, C H Ye, G Z Shen, U K Gautam, C C Tang and D Golberg, Chem Commun., 2007, 4093; Y B Tang, H T Cong and...

Ngày tải lên: 20/03/2014, 13:12

... of b1 and , we compute a rst approximation of demand and supply growth rates, as et = x01,t and et = x02,t b2 Even if we know that and b2 are not convergent estimators d b s b of and ... exible8 Third, as is standard in the literature, we introduce in the AD/AS framework a bank lending channel working over and above the interest rate channel by assuming that bonds and loans are imperfect ... The nominal loan demand is: ld = p l il + b ib + y y, (3) with l , b and y standing for the loan interest rate, the bond interest rate and the income elasticities of loan demand respectively,...

Ngày tải lên: 22/03/2014, 23:20

... R Hernandez-Fernaud and E Salido Proteome changes in primary hyperoxaluria 4769 Proteome changes in primary hyperoxaluria ´ J R Hernandez-Fernaud and E Salido Fig Metabolic kidney (A) and liver ... peroxisomal (FP) and cytosolic (FC) fractions Densitometry of western blot bands was performed and units of selected proteins were calculated A representative western blot band for each protein ... protein expression within the liver and kidney of Agxt) ⁄ ) deficient mice 4768 ´ J R Hernandez-Fernaud and E Salido compared with wild-type controls by 2-DE separation and MS The analysis of specific...

Ngày tải lên: 23/03/2014, 03:20

... CD98 and galectin membrane trafficking P Dalton et al and formation of activated complexes with other proteins, in particular b1-integrin, galectin and CD147, has been proposed ... folding and for ligand–receptor interactions, and because CD98 is an N-glycosylated protein [29,30], in this study we investigated the effect of BFA on the expression and the function of CD98 and ... route to the plasma membrane that is independent of the classic secretory pathway through the trans-Golgi apparatus, which is used by most secretory and transmembrane proteins and can be inhibited...

Ngày tải lên: 23/03/2014, 09:20

... cofactor Membranes from several independent standard targeting reactions with or without ATP were pooled and analyzed by EPR spectroscopy (see Materials and methods) Total INVs (130 lg) from five standard ... and has a globular folded structure, and thus is not available for membrane insertion The exact topology of the membrane- inserted N-terminus of preHoloHiPIP remains to be determined (Fig 9) Membrane ... substrates before membrane insertion How could membrane insertion take place? Proteins may insert into the membrane in either a spontaneous or a catalyzed mode Our observation that a membrane potential...

Ngày tải lên: 23/03/2014, 21:20

... tagging proteins for immunodetection, for using chimeric proteins for cytological purposes, especially the analysis of membrane proteins and intracellular protein trafficking, and for monitoring and ... in proteins, to dissect structurefunction relationships, to monitor membrane protein complex assembly, and to analyze the membrane topology of (unfused) membrane proteins (reviewed in Manoil and ... (B) Similarly, pRS551 and pRS550 are the same plasmid with the MCS in opposite orientations, and so on Plasmids pRS415 and pRS551 (and the corresponding plasmids pRS528 and pRS550) are designed...

Ngày tải lên: 10/04/2014, 11:02

... tagging proteins for immunodetection, for using chimeric proteins for cytological purposes, especially the analysis of membrane proteins and intracellular protein trafficking, and for monitoring and ... applications: molecular characterization of ligands and receptors, and the cloning of novel ligands and receptors Although we focus on polypeptide ligands and their cell surface receptors, the same ... receptor or ligand fusion protein probes focused on the identification and cloning of previously unknown ligands or receptors.4 More recently it has been found that receptor and ligand fusion proteins...

Ngày tải lên: 10/04/2014, 11:02

... specimens and data analysis, and drafted the manuscript ZYZ and JF participated in the interpretation of data, conducted immunohistochemistry, and western blot analysis All authors read and approved ... p53, p21/WAF1, and MDM2 in HCC tissues than the corresponding adjacent hepatic tissues; the expression levels of the proteins in the former was 70.7%, 33.1%, and 52.5%, respectively and those in ... [24] and cutaneous squamous cell carcinoma [25], but is decreased in colorectal carcinoma [26] and ovarian carcinoma [27] Table 3: The expression of p53, p21/WAF1, and MDM2 in HCC tissues and...

Ngày tải lên: 18/06/2014, 15:20

... that capsid proteins, especially VP1 and VP2, are important for virus persistence and demyelination [1,3] In addition to these structural proteins, two proteins designated leader (L) and L* that ... envelope A singlestranded RNA is packaged in the shell that consists of four capsid proteins, VP1, VP2, VP3 and VP4 [13] Neutralizing epitopes have been identified [14-16], the nt and predicted AA ... contributions MT conceived this review and wrote the initial draft with KA under the direction of YO YO and TO modified, wrote and submitted the final draft All authors read and approved the final version...

Ngày tải lên: 19/06/2014, 22:20