membrane structure results in selective permeability

... deeper hydrolysis in channels [49-52], followed by fragmentation [48] In the current study, the presence of remaining shells with holes in the sbe1a RS indicates continuing difficulty in digestion ... of longer chains in b-LDs of amylose from sbe1a can be explained by branch points that tend to be closer to the non-reducing ends, so that longer internal chains result When debranching of b-LDs ... barrier to binding of bamylase Although most of the DP is from residual A chains [43], some DP chains from residual B chains would result from short B chains with short internal segments The incomplete...

Ngày tải lên: 11/08/2014, 11:20

... Therefore, we investigated successional development of nitrifying bacterial community structure and in situ nitrifying activities in biofilms when the biofilms were grown on rotating disk reactors ... analyzed by comparing with existing 16S rRNA gene database and used for probe and primer designing The fingerprinting techniques can be used to monitor microbial community changes in natural environments ... Nitrifying Bacteria Although the biofilm was cultured in the synthetic medium containing no organic carbon, the NH4+-oxidizing bacteria clusters were surrounded by a number of heterotrophs including...

Ngày tải lên: 05/09/2013, 08:40

Ngày tải lên: 08/10/2013, 21:20

... class East Asian individual could benchmark against Finally, we correlated measures of cognitive performance and brain structure In view of the increasing automation in brain structure measurement, ... valuable starting point from which to base future studies concerning brain aging in Asians Methods Participants The volunteers were members of the Singapore Longitudinal Aging Brain Study, a community-based ... Longitudinal magnetic resonance imaging studies of older adults: a shrinking brain J Neurosci 23, 3295–3301 Reuter-Lorenz, P.A., Lustig, C., 2005 Brain aging: reorganizing discoveries about the aging...

Ngày tải lên: 14/02/2014, 06:20

... same antigen– antibody interaction in the presence of increasing concentrations of NaCl In both experiments, binding intensities are represented in RU, and each data point shows the mean value ... bufferexposed IVIg to recombinant human IFN-c (D) Increased binding of low-pH buffer-exposed IVIg to human factor H In both panels, binding of the native IVIg is indicated by a solid line, and that of the ... mechanisms In addition to sepsis, there is an increasing number of emerging infectious diseases in which the severe generalized in ammatory reaction of the infected host is a major factor in the...

Ngày tải lên: 16/02/2014, 15:20

... modified LDL (1 mg proteinÆmL)1, incubated with 100 mM modifying agent or 50 lM EDTA, in NaCl ⁄ Pi, pH 7.4, for days at 37 °C) for up to 96 h in medium containing 10% lipoprotein-deficient serum (with ... 69–90 10 Tomkin GH & Owens D (2001) Abnormalities in apo B-containing lipoproteins in diabetes and atherosclerosis Diabetes Metab Rev 17, 27–43 11 Lyons TJ & Jenkins AJ (1997) Lipoprotein glycation ... Odani H, Shinzato T, Matsumoto Y, Usami J & Maeda K (1999) Increase in three a,b-dicarbonyl compound levels in human uremic plasma: specific in vivo determination of intermediates in advanced...

Ngày tải lên: 19/02/2014, 02:20

... Protein in- gel digestion and extraction of peptides Following SDS/PAGE and Coomassie blue staining, protein bands (approximately 7.5 lg protein each) were treated as described in [25] Protein cleavage ... recombinant Glg2p The recombinant Glg2p carrying a C-terminal His6 tag was purified close to homogeneity and incubated with UDPglucose At intervals (0, 5, 10, 15 and 20 min), aliquots of the incubation ... dominant protein band slightly decreased with incubation time suggesting a progressive glucosylation of the recombinant glycogenin (Fig 1) For a more detailed analysis, the dominant protein band...

Ngày tải lên: 19/02/2014, 16:20

... Glutamine incorporation in Caco-2 proteins determining the relative uptake of glutamine; (b) by searching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into ... labelling of 20 distinct proteins, which are indicated in bold NQ, No glutamine-containing peptides in spectrum peaks C-term, C-terminal part of protein; N-term, N-terminal part of protein Spot ... synthesis of arginine plays an important role in maintaining arginine homeostasis in postweaning growing pigs J Nutr 127, 2342–2349 61 Quan J, Fitch MD & Fleming SE (1998) Rate at which glutamine enters...

Ngày tải lên: 20/02/2014, 01:20

... subunit, affecting its trypsin digestion and cross-linking pattern In addition, a change in the e conformation was previously seen, again by trypsin digestion and cross-linking, following dicyclohexylcarbodiimide ... contained 20 mM Tricine instead of succinic acid Assuming complete equilibration of the K+ during the h preincubation (see above), the value of the K+/valinomycin diffusion potential determined ... they are integrated into the chromosome by homologous recombination This results in an exchange between the incoming DNA and the corresponding chromosomal DNA which is lost in the process In this...

Ngày tải lên: 21/02/2014, 03:20

... outputs is an instance; each rule copies idiosyncratic information from the input frame while avoiding copying information which can still be inherited; each rule takes as input a single-word lexical ... the complete mode of inheritance in determining the case for a finite verb's subject, we would have a contradiction, but by using the normal mode, we find the mo~e local (NOMINATIVE) value for ... here involve the role of the PS rules in specifying linear order of constituents, whether the linking rules of G P S G (which we still employ) could ever be pushed into the lexicon, and whether in...

Ngày tải lên: 21/02/2014, 20:20

... TNFa-induced U937 cell apoptosis by maintaining mitochondrial integrity and function [43], and ricin-induced apoptosis of U937 cells by maintaining an intracellular reducing condition by acting ... factor) binds to the Jun domain, and that the binding is dependent on the amount of glucocorticoid [13] In addition, this binding results in exhibition of anti -in ammatory effects through inactivation ... was measured during in vitro incubation in culture medium using reverse transcription (RT)/PCR The results are shown in Fig Under conditions in which a house-keeping gene (b-actin) mRNA was constitutively...

Ngày tải lên: 22/02/2014, 07:20

... writing, and the way of thinking in L1 influenced the pattern of the text organization in L2 writing (James, 1980) Many other researchers have had similar findings According to Schumann (1998), ... again, changing some structures and also writing missing things such as subject pronouns 24 students out of 24 highlighted that the use of their first language, Spanish, hindered their writing in ... high school to college regarding thinking processes, this method is relevant in their beginning level courses In high school, some students are only asked to reach the minimum level to pass By the...

Ngày tải lên: 24/02/2014, 18:20

... is indicated in the right margin of the tree and the group including the protamine-like proteins and the germinal H1 histones is highlighted in gray in the tree, where the protamine-like protein ... fastmoving fraction Figure 1D shows a summary of the sequencing results obtained The N-terminal domain, obtained by direct sequencing of the whole protein, was found to contain a 20 amino acid ... specificity resides in the intrinsically disordered domains of their N- and C-terminal tails In the case of the PL-I proteins, there is a substantial increase of arginine within these regions (see...

Ngày tải lên: 08/03/2014, 08:20

... O-linked sugar region, very rich in serine, a transmembrane region between amino acids 1690–1705 and, finally, a cytoplasmic domain This cytoplasmic domain includes a region homologous to the internalization ... corresponding domain) A, Class A cysteine-rich repeats; B, class B cysteine-rich repeats; C, cytoplasmatic domain; EGF, epidermal growth factor precursor homology domain; LBD, ligand binding domain; ... of the last nymphal instar, steadily declining along the instar, and reaching the lowest values of the instar before the imaginal moult (Fig 3A) In the adult, mRNA levels remained low or even still...

Ngày tải lên: 16/03/2014, 14:20

... of four time points (120, 180, 240, and 320 min) The lines indicate the fitting of the data to the two-binding-site model Surface density analysis of the hydrolysis of crystalline cellulose Figure ... (k ¼ v ⁄ A) towards crystalline cellulose samples was plotted against surface density as shown in Fig The k values of all samples declined linearly with increase in q when a logarithmic scale ... and from the nonreducing end of each chain are compared among cellulose Ia, cellulose Ib, and cellulose IIII in Fig The structure of cellulose IIII results in a lower packing density than that...

Ngày tải lên: 23/03/2014, 09:21

... detection The results obtained by the method described in Section 3.2 are shown in Table The table shows five kinds of results: ¯ results obtained without dependency structure (in the first chunking step) ... determining the beginnings of clauses is difficult in a single process because all features mentioned above are local information Therefore, the global information is also used to detect the beginning ... step) ¯ results obtained with dependency structure analyzed for the open test (in the second chunking step) ¯ results obtained with dependency structure analyzed for the closed test (in the second...

Ngày tải lên: 23/03/2014, 18:20

... liposomes using one of the menaquinones of W succinogenes To elucidate the function of PsrC, which is thought to bind quinone, certain amino-acid residues of this subunit were replaced using site-directed ... dehydrogenase were incorporated into liposomes containing one of the quinones indicated (10 lmolỏg phospholipidA1) The activities of polysulđde respiration of proteoliposomes containing MM were more ... MK4 or vitamin K1 were also incorporated However, if MM was incorporated instead, the activity was as low as that of proteoliposomes prepared without added quinone In liposomes containing fumarate...

Ngày tải lên: 24/03/2014, 03:21

... The data reported in the present study, and obtained in living cells, confirm the finding obtained in reconstituted proteoliposomes [11,12] and isolated membranes [29,30] indicating that Pgp and ... crossing a lipid barrier located in the plasma membrane The main intracellular accumulation site of rhodamines inside cells is the mitochondria This accumulation could be eliminated by poisoning ... the initial fast phase of fluorescence in the Pgp-over-expressing cells or fluorescence quenching in wild-type cells (Fig 5) Inhibition of Pgp with cyclosporine A caused a parallel increase in the...

Ngày tải lên: 29/03/2014, 08:20

... protein concentration for the binding assay was determined using 25 nM [3H]PonA and increasing amounts of proteins in individual incubations The percentage of specific binding increased in a protein ... binding Membrane fractions containing 100 lg protein in 100 lL buffer (pH 7) were incubated with 25 nM [3H]PonA at various temperatures j, total binding; d, specific binding; s, nonspecific binding ... Thus, the binding sites might be on integral membrane proteins Phase partitioning in the detergent Triton X-114 Integral membrane proteins are generally classified into two categories, proteins that...

Ngày tải lên: 30/03/2014, 15:20

Ngày tải lên: 24/04/2014, 17:23