members of the nuclear suppliers group as of june 2006

... represent the SEM analyzed As shown in Fig 2A, the protein level of Nup358, a component of the cytoplasmic filaments of the NPC, gradually increased as muscle cell differentiation proceeded All other ... (GM), these cells grow as mononuclear myoblasts, although they fuse to form multinuclear myotubes when cultured in low-serum differentiation medium (DM) Dynamic remodeling of the nuclear Roles of ... GTPase, RanGTP ⁄ GDP, across the nuclear envelope regulates the binding and release of cargo by transport factors RanGTP is abundant in the nucleus as a result of the activity of RCC1, a guanine nucleotide...

Ngày tải lên: 06/03/2014, 01:20

... sector the main choice of employment was with the state c) Third, Vietnam is a single party ruling country with the Communist Party of Vietnam (CPV) as the leading force of the government Most of the ... standards Both make use of the quality and quantity of the employees’ outputs as a measure of performance Performance is measured vis-a-vis the targets set at the beginning of the year in accordance ... and incompetent officials and the mass media The cost of the civil service as streamlined and measures were taken to decrease the use of indirect incentives and “secret” perks The Chinese civil...

Ngày tải lên: 25/12/2013, 06:16

... 1D As expected, the electron density is strong along the peptide main chain with well-defined carbonyl and amide backbone groups The one exception to this is the location of the amide group of ... fits into the hydrophobic alcove and allows the charged head group access to the solvent on the other side The extended side chain of Arg206 allows for the same favourable interactions as a Lys ... competes for the importin-a binding site, reducing binding affinity for cargo proteins and helping to facilitate the release of the cargo within the nucleus [10,11] The removal of the autoinhibitory...

Ngày tải lên: 14/02/2014, 19:20

... disturbing the protein homeostasis As a defence mechanism, HSFs induce the synthesis of HSPs that act as molecular chaperones through binding to the hydrophobic surfaces of unfolded proteins, thereby ... development of the lens and maintenance of the olfactory epithelium [102] The first evidence for a developmental function of HSF4 was provided by population genetic studies where mutations of the Hsf4 ... by the recent findings that different members of the HSF family are able to interact, both structurally and functionally, thereby impacting the actions of their partners The interplay among the...

Ngày tải lên: 18/02/2014, 04:20

... in the central cylinder of the root, but not in the lower parts of the hypocotyl (a) Rosette leaves were stained in the vascular tissue of the leaf blade, whereas the petiole and older parts of ... staining within the vascular tissue at the end of the leaf blade, whereas the basal parts close to the petiole remained almost unstained We observed clear GUS expression in the primary root of 7-day-old ... parts of the hypocotyls (Fig 4G) In rosette leaves, we observed expression in the vascular tissue of the leaf blade, whereas, interestingly, in older parts of the midrib, no GUS expression was detectable...

Ngày tải lên: 18/02/2014, 06:20

... respectively The last member of the PDGF family was published in 2001, again by three independent groups, and named PDGF-D and SCDGF-B [10–12] The gene was identified by BLAST search in the EST database ... induced neovascularization of the avascular corneal tissue In these experiments, PDGF-C was as potent as PDGF-BB and more potent than PDGF-AA PDGFC affects the endothelial cells lining the blood ... resulting in perivascular lymphoid cell infiltrates of the lung and fibrosis in the liver [110] Conclusions The novel members, PDGF-C and -D, of the PDGF subfamily of the cystine knot family of growth...

Ngày tải lên: 19/02/2014, 07:20

... of the nAChR [19,42] was adopted, as were the other kinetic constants previously derived [23] This concerns in particular the microscopic lipid dissociation constant, KL, of the nAChR under the ... kinetic modelling was to explain the ‘leftward shift’ of the dose–response curves of tadpole narcosis relative to those of nAChR agonist binding enhancement It is known that the members of ligand-gated ... has recently also been documented by ESRspectroscopy for the nAChR [26] In the case of b-hydroxybutyrate dehydrogenase there was a 15-fold difference in macroscopic binding constants between the...

Ngày tải lên: 19/02/2014, 18:20

... cytoplasm, a considerable fraction of the a subunit can be found either in the cytoplasm or the nucleus [35,36] The involvement of eEF1A in the regulation of nuclear processes includes the accumulation ... Western blotting The filter was assayed using anti-eEF1A mAb, as described in the Materials and methods The position of the eEF1A protein was confirmed by revealing the presence of the Ran–GTP protein ... confirmed the nature of this protein (data not shown) Moreover, the spectrum reported in the figure showed the occurrence of a series of signals that were not interpreted simply on the basis of the...

Ngày tải lên: 21/02/2014, 00:20

... the binding affinity for HSEstep was threefold lower than that for HSE3P The amount of hHSF2 polypeptide used in the assay was fourfold lower than that of hHSF1 polypeptide, and the addition of ... ng of firefly luciferase reporter plasmid, 10 ng of pRLTK control plasmid containing the Renilla luciferase gene driven by the HSV-TK promoter (Promega), 10 or 100 ng of hHSF–VP16 expression plasmid, ... with the human c-crystallin promoters These results show that the configuration of nGAAn units in the promoter is important in determining which hHSF members are involved in the regulation of the...

Ngày tải lên: 07/03/2014, 00:20

... not the b domain The distinguishing feature of members of the meiotic clade of AAA ATPases is the SRH motif, which differs from that of other AAA ATPases [24] The pair of Arg residues in the ... alignments of some of the proteins listed in the PFAM database that contain the Vps4 C-terminal oligomerization domain (PF09336) The sequence of the SRH of each protein is also shown The sequence of the ... important One of these, the TRP sequence, includes the start of the C-terminal helix as well as the structured loop between the 1440 ATPase domain and the C-terminal helix and in the 3D structure...

Ngày tải lên: 07/03/2014, 05:20

... tyrosine-phosphatase inhibitors to the cells in culture In the case of the multiprotease approach (Fig 1A), we added 100 lm of the tyrosine-phosphatase inhibitor BiPy [31] to the culture medium of mouse-neuroblastoma ... cells at the beginning of homogenization Both inhibitors were then present throughout the preparation of nuclei and nuclear envelopes, although, in contrast to the phosphatases, the kinases should ... from the gel and further analyzed In-gel digestion of these bands was done in parallel with four enzymes: trypsin, elastase, proteinase K, Tyrosine-phosphorylation of emerin and thermolysin The...

Ngày tải lên: 07/03/2014, 12:20

... Molecular modeling of the 3D structure of the mSMOl isoform was thus carried out in order to test the hypothesis that the 277–307 region and the protein domain encoded by the exon VIa could be ... mammalian-specific function of SMO (e.g nuclear targeting of the mSMOl isoform) Inspection of the mSMOl modelled structure (Fig 2) indicates that both regions are located on the tip of the FAD-binding domain, ... sequence comparison of members of the spermine oxidase (SMO) and polyamine oxidase (PAO) family (A) Amino acid sequence alignment of the SMO and PAO proteins (B) Alignment of the deduced amino...

Ngày tải lên: 07/03/2014, 17:20

... 1989 65 Report of the Meeting on the Assessment of the Meteorological Aspects of the Fourth Phase of EMEP, Sofia, Bulgaria, 27 and 31 October 1989 66 Summary Report on the Status of the WMO Global ... Concentrations as Measured at BAPMoN Sites for the Year 1982 November 1984 (WMO TD No 12) 24 Final Report of the Expert Meeting on the Assessment of the Meteorological Aspects of the Second Phase of EMEP, ... greenhouse gases (as well as other gases) at the Japan Meteorological Agency, Tokyo, are receiving most of the represen tative data within their terms of reference The QA/SAC for the Americas, USA, has...

Ngày tải lên: 15/03/2014, 23:20

... between the two proteins (as indicated by the values of DGwater, Cm and Tg) hence arise from the changes in the surface of the protein because of the interactions of the cofactor with the protein ... comparable, the Tg values of the proteins vary slightly; there is a difference of almost C between the Tg values of the proteins, the value being higher for the holo form There have been contrasting ... molecular masses of apo-ACP and holo-ACP were estimated on the basis of the linear regression of the fraction number of the molecular mass markers cytochrome c (CyC), carbonic anhydrase (CA),...

Ngày tải lên: 16/03/2014, 10:20

... Elmer) Washing of precipitated sample was performed using the same protocol as that described for the kinase time-course assay The assay was again repeated with the inclusion of 10 mm MnCl2 in the ... Intensity of cytoplasm Ratio of Translocation = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software ... components of the NF-jB signaling pathway in single cells [11,12] These results agreed with in silico simulations of the downstream region of the pathway that was modeled, as well as suggesting that the...

Ngày tải lên: 16/03/2014, 11:20

... analyzed the effect of S100A8/A9 on the kinetics of oxidase activation The oxidase activity elicited in the cell-free system was the reflect of the extent of oxidase activation The experiment of Fig ... determined for each assay of oxidase activation [12] The rate of O2– production by the activated NADPH oxidase was calculated from the rate of the superoxide dismutase-inhibitable reduction of ferricytochrome ... than 98% of the reduction of cytochrome c was inhibited by SOD, therefore corresponding to the production of O2– The oxidase activity was expressed in mol of O2– generated per s and per mol of membrane-bound...

Ngày tải lên: 18/03/2014, 01:20

... the wall of the binding pocket and allow the access of bulky residues, like Arg and Lys, whereas the basic side chain of these residues is stabilized by Asp189 (chymotrypsin numbering) near the ... from the 3¢ UTR region of PaTry2 However, the 3¢ UTR sequence of PaTry4 differed to those of the other trypsins in more than 36% of its nucleotides All ORFs started at the first ATG codon of the ... 1) designed to flank the 5¢ UTR and 3¢ UTR of the different cDNAs allowed the amplification of several fulllength cDNAs Eleven clones of expected size were sequenced Three of them did not have suitable...

Ngày tải lên: 23/03/2014, 03:20

... and other objects with which she was then familiar." A lady who was a fellow passenger of the Pickards on their homeward voyage was struck by the gentle management of the mother and the easy docility ... WARE] Of all the saints in the calendar of the Church there is no name more worthy of the honor than that of Mary Lovell Ware The college of cardinals, which confers the degree of sainthood for the ... proved, and the child, then at the age of thirteen, was called home and installed in the sick-room as nurse This was the beginning of sorrow The mother lingered through the winter and died in the following...

Ngày tải lên: 23/03/2014, 05:20

... wave was formed The high temperature of the gas cap in front of the comet’s leading edge caused evaporation and fragmentation of the comet’s surface Consequently, the deuterium density in the gas ... most of the incident energy received is transformed into aerodynamic heating of the gas cap as opposed to the heating of the body surface Of interest to us here are the fusion reactions and massenergy ... A(Mc/~c)*‘~, the effective surface area of the comet, and then dividing by L, the latent heat of vaporization, then indicates how much mass has ablated from the comet surface As compact as equation...

Ngày tải lên: 23/03/2014, 13:20