medicinal chemistry of antisense oligonucleotides

... reports of an impairment of cellular uptake of antisense oligonucleotides after covalent attachment of peptides [9,11,28] These authors nevertheless found an enhanced biological activity of the ... role of structure forming properties of the peptide upon the entry of oligonucleotides into the cell interior (Figs and 7) Conjugation of the 15-mer PTO to the Antennapedia-peptide V, one of the ... conjugation of phosphorothioate oligonucleotides to cationic, cell-penetrating peptides, irrespective of peptide structural properties, does not substantially alter the ability of oligonucleotides...

Ngày tải lên: 23/03/2014, 21:21

... Page of References Stahel RA, Zangemeister-Wittke U: Antisense oligonucleotides for cancer therapy-an overview Lung Cancer 2003, 41:S81-S88 Biroccio A, Leonetti C, Zupi G: The future of antisense ... the percentage of fluorescent cells out of the total number of cells Fluorescence was detected from the FAM labeled on ASODNs at 488-nm excitation In vitro cell toxicity assay of the f-HNT-ASODN ... PBS, dehydrated through a series Page of of alcohol concentrations (30%, 50%, 70%, 90%, 100%), embedded in Epon, and sliced to a thickness of 70 nm Images of the sliced images were recorded at...

Ngày tải lên: 20/06/2014, 23:20

... Series Editors Dr Prof Franco Cataldo Director of Lupi Chemical Research Institute Via Casilina 1626/A 00133 Rome, Italy Professor Paolo Milani University of Milan Department of Physics Via Celoria, ... 20133, Milan, Italy Volume 1: Medicinal Chemistry and Pharmacological Potential of Fullerenes and Carbon Nanotubes Volume Editors Dr Prof Franco Cataldo Director of Lupi Chemical Research Institute ... Years of Promises: Fullerene in Medicinal Chemistry Tatiana Da Ros Abstract Many biological activities have been envisioned for fullerenes and some of them seem to be very promising The lack of...

Ngày tải lên: 17/02/2014, 05:20

... %) Figure RNA The effect of antisense LNA on reverse transcription of HIVThe effect of antisense LNA on reverse transcription of HIV1 RNA An equimolar amount (1pmol) of HIV RNA and LNA oligo ... 150 200 Time of reaction (in minutes) Figure The effect of antisense LNA versus RNA and DNA on dimerization of HIV-1 RNA The effect of antisense LNA versus RNA and DNA on dimerization of HIV-1 RNA ... DIS LNA antisense DIS PBSD DNA antisense PBSD DIS LNAzyme PBSD DIS DNAzyme Figure Inhibition of intracellular HIV-1 production in the presence of antisense LNA and LNAzymes Inhibition of intracellular...

Ngày tải lên: 13/08/2014, 09:21

... GGC CAA ACC UCG GCU UAC CU 31 25 25 20 20 25 25 25 20 20 52 44 36 25 40 52 40 40 45 60 12 OF OF OF OF OF IF IF IF IF IF 1 *M23D(+02-18) has been described previously [18, 21] Methods Animals All ... 2OMe AO cocktails were complexed with Lipofectin (Life Technologies) at the ratio of 2:1 lipofectin:AO and used in a final transfection volume of 500 µl/well of a 24-well plate as per the manufacturer's ... RT-PCR 4of shortened transcripts induced in the presence of background alternative splicing RT-PCR of shortened transcripts induced in the presence of background alternative splicing RT-PCR pattern of...

Ngày tải lên: 14/08/2014, 19:22

... Presented by Eric Allain – Assistant professor at Alalachian university http://vimeo.com/2993351 Presented by Prof S Dasgupta, Dept of Chemistry, IIT Kharagpur Institute of Technology at Kharagpur, India ... polarimetry Magnitude of rotation depends upon: the nature of the compound the length of the tube (cell or sample container) usually expressed in decimeters (dm) the wavelength of the light source ... POLARIMETRY Measurement of optical activity in chiral or asymmetric molecules using plane polarized light Molecules may be chiral because of certain atoms or because of chiral axes or chiral...

Ngày tải lên: 21/07/2015, 16:18

... use of germanium has also led to the synthesis of the pure polymorph C of zeolite beta (BEC) even in the absence of the fluoride medium that is generally believed to assist in the formation of ... control of topological features such as pore size and channel dimensionality In particular, the expansion of the pore size beyond 10Å has been one of the most important goals in the pursuit of new ... chapters of this book A frequently used term in the field of microporous materials is “molecular sieves” [5] that refers to a class of porous materials that can distinguish molecules on the basis of...

Ngày tải lên: 23/04/2013, 14:23

... use of germanium has also led to the synthesis of the pure polymorph C of zeolite beta (BEC) even in the absence of the fluoride medium that is generally believed to assist in the formation of ... control of topological features such as pore size and channel dimensionality In particular, the expansion of the pore size beyond 10Å has been one of the most important goals in the pursuit of new ... chapters of this book A frequently used term in the field of microporous materials is “molecular sieves” [5] that refers to a class of porous materials that can distinguish molecules on the basis of...

Ngày tải lên: 23/04/2013, 20:25

... electronegativity of B & N about the same as C  electrical insulators Chemistry, Julia Burdge, 2nd e., McGraw Hill Insulated Nanowire Chemistry, Julia Burdge, 2nd e., McGraw Hill Properties of BN and C Chemistry, ... Albite = ¼ of Si replaced by Al; Na(AlO2)(SiO2)3 Anorthite = ½ of Si replaced by Al; Ca(AlO2)2(SiO2)2 Chemistry, Julia Burdge, 2nd e., McGraw Hill 10 Silicates Made of Individual Units • O of SiO4 ... layer of fused rings • multi-walled nanotubes (MWNT) have concentric layers of fused rings Tro, Chemistry: A Molecular Approach 28 Nanotubes Tro, Chemistry: A Molecular Approach 29 Nanocars Tro, Chemistry: ...

Ngày tải lên: 28/11/2013, 01:12

... vinegar weigh TRANSLATION muối ăn giấm cân nặng Los Angeles County Office of Education Office of Science Consultants- 11/04 STRUCTURE OF MATTER polymer pressure principle product proton random reactant ... investigate influence ảnh hưởng kilometer internal bên laboratory Los Angeles County Office of Education Office of Science Consultants- 11/04 This sheet should be accompanied by the Periodic Table ... lực mạnh time (t) mặt trời toward TRANSLATION độ căng, căng thẳng Los Angeles County Office of Education Office of Science Consultants- 11/04 two-dimensional This sheet should be accompanied by...

Ngày tải lên: 16/01/2014, 23:20

... binding of ligands on the iron of Fe(porphyrin))13G10 complexes: (A) binding of H2O2 on the iron of 13G10-(Fe(ToCPP), (B) binding of two CN± ligands on the iron of 13G10-(Fe(ToCPP), (C) binding of ... presence of varying concentrations of imidazole (Fig 6, Table 4) First of all, the addition of increasing amounts of imidazole, from to 150 mM, caused a slight increase of the peroxidase activity of ... 13G10-a,b-1,2-Fe(DoCPP) a plateau value of 0.72 lM ABTS oxidized per for a concentration of imidazole of 50 mM (Fig 6) For concentrations of imidazole higher than 100 mM the rate of oxidation of ABTS started to decrease...

Ngày tải lên: 21/02/2014, 03:20

... cellulose Technical processes for recovery of whey proteins often make use of such substances or of a combination of heat and pH adjustment When milk is heated, some of the whey proteins denaturate and ... properties of the milk The degree of heat treatment must be carefully chosen Physical properties of milk Appearance The opacity of milk is due to its content of suspended particles of fat, proteins ... acidity The titrable acidity of milk is the amount of a hydroxyl ion (OH–) solution of a given strength needed to increase the pH of a given amount of milk to a pH of about 8.4, the pH at which...

Ngày tải lên: 08/03/2014, 23:20

... group of studies investigated the fate of phosphonates that are already present in the influent of the WWTP For a 13-day field study a total amount of 117 mol of DTPMP was found in the influent of ... surface reaction follows: adsorption, dissolution of minerals, remobilization of metals, precipitation of phosphonates and inhibition of precipitation of minerals are covered In the degradation section ... basis for a refined risk assessment The aim of this review is therefore to provide an overview of the current knowledge of the environmental chemistry of phosphonates It concentrates on polyphosphonates,...

Ngày tải lên: 16/03/2014, 00:07

... overview of catabolic pathways Metabolism of fatty acids Metabolism of glucose (glycolysis) Conversion of pyruvate to acetyl coenzyme A Anabolism of fatty acids from CH3COSCoA Anabolism of glucose ... Biogenesis of phenols through shikimic acid Biosynthesis of shikimic acid O COO OH + PO PO O OP COO O HO OH OH OH COO HO COO -H2O O OH OH NADPH shikimic acid OH HO OH Biosynthesis of aromatic ... Condensation of isoprene units Formation of acyclic terpenes -H+ OPP OPP OPP H OPP OPP geranyl OPP OPP farnesyl OPP OPP tail-tail dimerization OPP geranylgeranyl OPP squalene Formation of monoterpenes...

Ngày tải lên: 18/03/2014, 20:20

... closed because of the probability of one occurring In spite of a demand for the product, manufacturers are reluctant to engage in the production of the material because of the history of problems ... Out of the atomic theory developed by John Dalton and other chemistry pioneers in the nineteenth century grew a number of important concepts essential to an understanding of all areas of chemistry, ... scale of atomic weights (as the weighted average of all forms, or isotopes, of a particular element found in nature) has been developed The base of this scale is the assignment of a mass of 12.0000...

Ngày tải lên: 22/03/2014, 09:20

... Borderline N3-, -CN, RNH2, Br- Soft I-, RS-, RSH, R3P, R2S Soft = The Chemistry of C-C π-Bonds - 38 - Examples of addition of HARD and SOFT nucleophiles:  Addition of thiolate anion: O  SPh PhSH, ... The Chemistry of C-C π-Bonds - - Alkene and Alkyne chemistry I  Alkene chemistry Structures and influence of orbitals on reactivity: How alkenes react and why? Typical reactions of alkenes: ... made by the Wittig reaction (Prof Donohoe’s course) O H R P Ph carbonyl ylid Ph Ph R O P Ph Ph Ph  The Chemistry of C-C π-Bonds - - Typical modes of reaction of alkenes [1] Nucleophilic Alkenes...

Ngày tải lên: 22/03/2014, 20:20

... EDITION Physical chemistry of macromolecules is a course that is frequently offered in the biochemistry curriculum of a college or university Occasionally, it is also offered in the chemistry curriculum ... term: Physics, Chemistry, Biology, and so forth; now we have departments with two terms of combined subjects: Chemistry and Biochemistry, Biochemistry and Molecular Biophysics, Chemistry and Chemical ... the configuration of macromolecules They are standard chapters for both a course of polymer chemistry and a course of biophysical chemistry Chapters 13 through 17 describe some of the important...

Ngày tải lên: 23/03/2014, 19:20

... Inside Cover MEDICINAL PLANTS OF MYANMAR Compiled by MINISTRY OF HEALTH DEPARTMENT OF TRADITIONAL MEDICINE Contents Kin-mun-gyin Ma-ya-gyi Kyet-thun-ni ... jaundice, insomnia and loss of concentration 300 ml per day of decoction is used for child in indigestion Expressed juice of fresh leaf is orally used in insect bite Ash of fruit is externally used ... Dosage Fresh leaves are used in the preparation of medicated paste by the ratio of about 30% Such paste is orally taken in a dose of 0.5 gm to gm jelly of fresh leaf is externally used as skin lotion...

Ngày tải lên: 29/03/2014, 15:20

Ngày tải lên: 29/03/2014, 17:20

Ngày tải lên: 01/04/2014, 10:40