mechanisms of epigenetic gene silencing
Báo cáo y học: " Reduced levels of two modifiers of epigenetic gene silencing" ppsx
Ngày tải lên :
09/08/2014, 22:23
... to ensure the production of a standard phenotype in spite of environmental disturbances is called canalization [30] Our studies show that modifiers of epigenetic gene silencing are fundamental ... density (Additional file 3), suggesting that much of the gene dysregulation in mutant mice is targeted to a subset of genes with characteristic epigenetic features These promoter regions may harbor ... number of genes in yeast has been shown to be associated with changes in the epigenetic state of their promoters [26-28] +/+ -/+ Relative mRNA level 16 14 12 10 Figure Variable expression of Mas1...
- 10
- 380
- 0
Báo cáo y học: "Reduced levels of two modifiers of epigenetic gene silencing, Dnmt3a and Trim28, cause increased phenotypic nois" pdf
Ngày tải lên :
09/08/2014, 22:23
... to ensure the production of a standard phenotype in spite of environmental disturbances is called canalization [30] Our studies show that modifiers of epigenetic gene silencing are fundamental ... density (Additional file 3), suggesting that much of the gene dysregulation in mutant mice is targeted to a subset of genes with characteristic epigenetic features These promoter regions may harbor ... number of genes in yeast has been shown to be associated with changes in the epigenetic state of their promoters [26-28] +/+ -/+ Relative mRNA level 16 14 12 10 Figure Variable expression of Mas1...
- 10
- 297
- 0
Báo cáo y học: "Quantifying the major mechanisms of recent gene duplications in the human and mouse genomes: a novel strategy to estimate gene duplication rates" docx
Ngày tải lên :
14/08/2014, 08:20
... NEW gene set Click geneforgeneset.s toFAM2geneset Ks of the gene pairs in the NEW hereisdataKa/Kset Ks ofgene/Kset.pairs in the NEW gene set Distribution of distribution gene set Mouse FAM 2gene ... the relative importance of different mechanisms of gene duplication and dynamics of duplicated genes generated by these different mechanisms Our results show that genes generated by unequal crossover ... number of genes in TAGs in each case minus the number of initial singleton genes, which can be estimated as one half of the number of genes in twocopy gene families refereed research We paired genes...
- 11
- 357
- 0
Báo cáo khoa học: Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco pot
Ngày tải lên :
22/03/2014, 21:20
... activity of the transgene When transcription of the transgene was partially repressed by methylation of transgene promoter sequences, siRNAs targeting the coding region of the transgene disappeared ... quantity of transgene mRNA in S44-end plants 1700 Our results indicate an example in which the transgene is overexpressed as a result of the interaction of RNA silencing The instability of transgene ... analysis of the transgene sequence (A) Degree of cytosine methylation at the END1 region of the El2 promoter (B) Degree of cytosine methylation at the END2 region of the El2 promoter (C) Degree of...
- 9
- 387
- 0
Báo cáo hóa học: " Prevention of hyperglycemia-induced myocardial apoptosis by gene silencing of Toll-like receptor-4" potx
Ngày tải lên :
18/06/2014, 16:20
... Data shown are representative of experiments Page of Figure Inhibition of caspase-3 after gene silencing of TLR4 (A) Suppression of Fas expression in the hearts of STZ mice treated with TLR4 ... http://www.translational-medicine.com/content/8/1/133 Figure Suppression of TLR4 and prevention of apoptosis by gene silencing of TLR4 (A) Suppression of TLR4 expression in the heart of STZ mice treated with TLR4 siRNA Diabetes ... whether knockdown of TLR4 may suppress apoptosis of cardiomyocytes in STZ-mice First, we validated in vivo gene silencing of TLR4 siRNA in myocardial tissue After infusion of TLR4 siRNA, the...
- 8
- 488
- 0
báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx
Ngày tải lên :
10/08/2014, 10:21
... investigated Page of 11 Construction of shRNA expression vector for MTA1 According to principle of shRNA, enzyme inciding site of vector pGenesil-1 and exon of MTA1 (GeneBank, No NM004689) in GeneBank, ... the molecular level, the consequence of ER activation appears to be alterations in transcriptional activity and expression profiles of target genes A number of genes, including cyclinD1, are regulated ... cut off by HindIII and XbaI lane 4: pGenesil-1/MTA1 shRNA(pGM2) plasmid was cut off by BamHI and HindIII lane 5: pGenesil-1/MTA1 shRNA(pGM2) plasmid was cut off by BamHI and XbaI lane 6: pGenesil-1/MTA1...
- 11
- 281
- 0
báo cáo khoa học: " Effects of RNA interference-mediated gene silencing of JMJD2A on human breast cancer cell line MDA-MB-231 in vitro" pptx
Ngày tải lên :
10/08/2014, 10:21
... and the establishment of local epigenetic state and chromatin structure required for proper induction of ER responsive genes JMJD2B which interacts with ERa and components of the SWI/SNF-B chromatin ... tumor suppressor gene ARHI in breast cancer Oncogene 2006, 25:230-239 doi:10.1186/1756-9966-30-90 Cite this article as: Li et al.: Effects of RNA interference-mediated gene silencing of JMJD2A on ... Sen GL, Blau HM: A brief history of RNAi: the silence of the genes FASEB J 2006, 20:1293-1299 Katoh M, Katoh M: Identification and characterization of JMJD2 family genes in silico Int J Oncol 2004,...
- 9
- 359
- 0
Báo cáo y học: " Selective gene silencing by viral delivery of short hairpin RNA" ppsx
Ngày tải lên :
12/08/2014, 01:22
... delivery of siRNAs for antiviral therapy Gene Ther 2006, 13:553-558 93 Hofmann A, Kessler B, Ewerling S, Kabermann A, Brem G, Wolf E, et al: Epigenetic regulation of lentiviral transgene vectors ... env genes are split between two viral genomes The duo allows co-propagation of two different transgenes, which offers both a back-up therapeutic opportunity, should the effect of the first gene ... manageable amounts of publications Vectors based on baculovirus can transport large amounts of genetic data leaving copious space for creative combination of gene therapy and silencing vectors...
- 11
- 266
- 0
Molecular analysis of hibiscus chlorotic ringspot virus coat protein mediated suppression of gene silencing
Ngày tải lên :
16/09/2015, 08:31
... 1.1 Gene silencing 1.1.1 Discovery of gene silencing 1.1.2 Induction of gene silencing 1.1.3 Mechanisms of gene silencing 1.1.4 Systemic silencing ... underlying mechanisms of gene silencing and related silencing responses Virus-encoded gene silencing suppressors offered an excellent tool for dissection of the gene silencing process Gene silencing ... et al., 2002) 1.1.3 Mechanisms of gene silencing 1.1.3.1 Genes conserved in the gene silencing pathway Through genetic screening of PTGS defective mutants, several sets of genes required for PTGS...
- 187
- 286
- 0
Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
Ngày tải lên :
31/10/2012, 15:12
... (1.8-fold of control) in the level of phosphorylated JNK 1&2, whereas 24hr exposure to hypoxia resulted in a decrease (0.4-fold of the control) Figure Expression of three representative genes by ... detected for several other genes Table Slope of standard curve and correlation coefficient of representative genes by Semiquantitative RT-PCR after 24 hours exposure to hypoxia Gene JNK1 PTEN Caspase ... were calculated for each gene Table Comparison between mRNA and protein expression of representative genes and proteins after hypoxia treatment for 24 hours Name of gene JAR Protein expression...
- 9
- 499
- 0
Study on the Photolytic Mechanisms of Red 141 Dye Wastewaters with an 185nm Vacuum-UV lamp
Ngày tải lên :
05/09/2013, 09:08
... OH· indirect oxidation generated from the excitement of O2 OH· indirect oxidation generated from the excitement of H2O OH· indirect oxidation generated from the excitement of H2O2 Figure The driving ... differentiation of the contributions on the decomposition of Re 141 in aqueous solutions by UV direct photolysis, OH generated from the excitement of water, oxygen, and H2O2, respectively Development of the ... fundamental principles of the photochemical process of VUV-induced water treatment In this work, the treatment of dye wastewaters by the VUV-only and VUV/H2O2 process in a homogeneous liquid-phase...
- 9
- 470
- 0
ISOTOPE HYDROGRAPH SEPARATION FOR MODELING OF RUNOFF MECHANISMS OF ATMOSPHERICALLY DERIVED CHEMICAL AND RADIOACTIVE POLLUTANTS
Ngày tải lên :
05/09/2013, 09:08
... 22-23 of 2003 The amount of precipitation was 30 mm in total Figure shows the precipitation record of the studied event (Fig 2a) and the record of the river flow rate (solid line, Fig 2b) of the ... measurement, considering of their respective analytical errors A concept of two-component mixing was employed, in which it was assumed that runoff is composed of a mix of two components: one originating ... and by setting C1 and C2 as mentioned above, the fraction of new water evaluated for the main part of the runoff (0000 of Oct 22 to 1600 of Oct 23, 40 h in total) was found to be as shown in Fig...
- 10
- 439
- 0
Mechanisms of Unstable Nitrite Inhibition of Aerobic Phosphate Uptake
Ngày tải lên :
05/09/2013, 09:38
... some of PAOs can that, the inhibitory effect of nitrite will be reduced Hence, the purpose of this study is to examine the effect of anoxic activity of PAOs on reduction of inhibitory effect of ... Cycle Operations of SBRs for Cultivation of the Enriched PAOs - 66 - Journal of Water and Environment Technology, Vol 6, No.1, 2008 Table - Composition of Synthetic Wastewater Methods of nitrite inhibition ... presence of nitrite was 13 mgP.gVSS-1.h-1 and the % activity of phosphate uptake was 39% On the other hands, in the case of AA with 1.9 mgN/gVSS of initial nitrite concentration, the size of inhibition...
- 11
- 271
- 0
Gián án gene silencing
Ngày tải lên :
27/11/2013, 20:11
... dsRNA siRNA RNA induced silencing complex (RISC) RISC = Protein + siRNA “Nhóm UD4” RISC siRNA Phá vỡ mRNA RNase “Nhóm UD4” RNA Gene Silencing 10 III Ứng D ụng – gene silencing Tạo hoa hồng xanh ... http://hopes.stanford.edu/n3609/drugssupplements-treatments/disease-mechanismsand-drugs-target-them/protein-aggregation/anti siRNA and miRNA Gene Silencing - From Bench to Bedside Edited by Mouldy Sioud Plant Methods Methodology-Virus-induced gene silencing ... – gene silencing Virus induced gene silencing (VIGS) “Nhóm UD4” 13 VIGS lựa chọn công cụ tạo im lặng nhanh chóng gene VIGS đòi hỏi tạo thành Virus tương ứng với gene tế bào vật chủ Sự lây nhiễm...
- 30
- 232
- 0
Transmission mechanisms of monetary policy the evidence
Ngày tải lên :
05/01/2014, 17:01
... cycle soon followed Transmission Mechanisms of Monetary Policy Traditional Interest-Rate Channels: expansionary monetary policy ir ↓, I ↑, Y↑ The interest rate channel of monetary transmission applies ... price for it relative to the cost of the facilities and equipment they are buying I ↑ because firms can buy a lot of new investment goods with only a small issue of stock The transmission mechanism ... follows: expansionary monetary policy Pe ↑, W ↑, C ↑, Y ↑ Tobin’s q and wealth mechanisms allow for a general definition of equity that includes housing and land An ↑ in house prices, which ↑ their...
- 25
- 2.3K
- 1
Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
Ngày tải lên :
12/02/2014, 10:20
... site was introduced on the end of the proposed synthetic trypsin inhibitor gene The sequences of the forward and reversed primers are shown on fig and of the synthetic gene is on fig Forward primer: ... expected (fig 5) Fig 5: Analysis of MCoTI-II gene on 5%polyacrylamide gel Lane 1: DNA Marker 100 bp (100-1031) (#SMO241/2/3) Lane : PCR product of MCoTI-II gene MCoTI-II gene was also successfully ... experiment were established as: 150ng of each primer; 200àM of dNTPs ;400ng of the purified total MCo DNA Cloning of the synthetic MCoTI-II The PCR amplified gene was double digested with NdeI...
- 9
- 497
- 0
Tài liệu Women''''s attitudes towards mechanisms of action of family planning methods: survey in primary health centres in Pamplona, Spain doc
Ngày tải lên :
13/02/2014, 06:20
... of action of some FP methods We did not state the mechanism of action of any FP methods and thus asked women to express their beliefs independently of their actual choices and independently of ... attitude questions were related to a general hypothetical method of FP In further sections women were asked about their knowledge of the mechanism of action of specific FP methods and if doctors ... attitudes for postfertilization effects The purpose of our study was to assess the attitudes about the mechanisms of action of FP in terms of whether these may influence a woman's decision to...
- 10
- 824
- 0
Tài liệu Báo cáo khoa học: Mechanisms of amyloid fibril formation – focus on domain-swapping doc
Ngày tải lên :
14/02/2014, 18:20
... core of amyloid plaques of various origins [115] Human stefin B (i.e cystatin B gene) mutations cause progressive myoclonus epilepsy of type 1-EPM1 [116,117], with signs of cerebellar neurodegeneration ... blocks only after partial unfolding of the a-helix from the body of the b-sheet (Fig 2C) Possible role of domain-swapping in the ‘off-pathway folding’ (OFF) model The OFF model for amyloid formation ... understanding of the importance of different parts of the molecule and their flexibility in the process of amyloid fibril formation often results more from a structural analysis of the amyloid...
- 20
- 755
- 0
Tài liệu Báo cáo khoa học: Survival mechanisms of pathogenic Mycobacterium tuberculosis H37Rv ppt
Ngày tải lên :
16/02/2014, 15:20
... Proton ATPasePump Inhibition of acidification of phagosome harbouring Mycobacteria Expression of virulence proteins of PE-PGRS family C O2– Fig Key factors of the survival mechanisms involved in the ... case of Mycobacterium smegmatis, enhanced permeability as a result of disruption of a mycolate ⁄ cording factor gene causes reduced growth both in vitro and in vivo [13] Disruption of the gene ... contribute to the pathogenicity of many bacterial species [46] Virulence genes of M tuberculosis Initial efforts aimed at identifying the genes involved in the pathogenesis of M tuberculosis involved...
- 12
- 783
- 0
Tài liệu Báo cáo khoa học: Mechanisms of obesity and related pathologies: Androgen deficiency and endothelial dysfunction may be the link between obesity and erectile dysfunction pptx
Ngày tải lên :
18/02/2014, 06:20
... with an increased risk of T2DM, hypertension and CVD; however, the exact underlying pathophysiological mechanisms by which obesity increases the risk of VD remains the subject of intensive investigation ... pathophysiological mechanisms of obesity-induced androgen deficiency and hypogonadism-induced obesity remain the subject of intense investigation Several hypotheses have discussed the relationship of androgen ... organic ED, in which approximately 43% of men with MetS had ED and the severity of ED increased with the components of the MetS [84] Interestingly, the severity of ED was positively associated with...
- 13
- 662
- 0