measurement of proteasomal dysfunction in cell models of dopaminergic degeneration

... and somatic cell counts in subclinical intramammary infections in milking goats in the UK Vet Rec 2007, 160(1):19-22 16 Min BR, Tomita G, Hart SP: Effect of subclinical intramammary infection on ... measurement of somatic cell count as indicator of intramammary infection in dairy goats Acta Veterinaria Scandinavica 2011 53:15 Submit your next manuscript to BioMed Central and take full advantage of: ... sampled at morning milking and one at evening milking Only clinically healthy animals without changes in udder consistency or milk appearance were included in the study All goats were in mid to late...

Ngày tải lên: 12/08/2014, 18:22

... protein (GFP)encoding gene under control of the HIV-1 LTR [17] In these Jurkat cell clones, GFP induction by HDACIs was evident only in a fraction of cells at 24 hours of incubation and increased ... replication in U1 and ACH-2 cells, and toxicity in uninfected JurPotencies Potencies of different HDACIs in terms of activation of HIV-1 replication in U1 and ACH-2 cells, and toxicity in uninfected ... toxicity in uninfected cells, and it induces cell death in infected cell cultures These results are consistent with a model in which BSO would favor the HIV-1 activating effects of HDACIs by lowering...

Ngày tải lên: 12/08/2014, 23:21

... Page of 12 Figure Endothelial cell response in sepsis (a) Leukocyte trafficking Activated endothelial cells (red-colored cells) express increased levels of E-selectin, P-selectin, intercellular ... 27 Kuhns DB, Alvord WG, Gallin JI: Increased circulating cytokines, cytokine antagonists, and E-selectin after intravenous administration of endotoxin in humans J Infect Dis 1995, 171:145-152 ... transcriptional start site are shown in the inset) E selectin induces rolling of circulating leukocytes VCAM-1 and ICAM-1 induce firm adhesion of leukocytes by binding to very late antigen (VLA4)...

Ngày tải lên: 13/08/2014, 21:21

... encapsulation of these sensors β-TC-6 cell was used as the cell line cultured in the final microfluidic device and in- situ measurement of three parameters including pH, oxygen and glucose concentration in ... CELL CULTURE MICROCHIP WITH BUILT -IN SENSOR ARRAY FOR IN- SITU MEASUREMENT OF CELLULAR MICROENVIRONMENT ZHANG LIN (B.Eng, ZJU, China) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING ... mimicking in- vivo cellular microenvironment Much work has been done in the integration of microfluidics and cell culture, however, measurement of the microenvironment in the microchannel remains...

Ngày tải lên: 03/10/2015, 11:36

... expression of an E2F variant containing the DNA binding motif but not the pRb-binding or transactivation domain or introduction of a competitor plasmid containing multiple E2F binding sites, preventing ... for binding to proteins containing LXCXE motifs while the pocket-domain and the C-domain are involved in binding E2F proteins Mdm2 (as well as c-Abl) binds to the C-domain All pocket proteins ... encoding a pRBbinding protein with properties of the transcription factor E2F Cell 70:337–350 Helin K, Harlow E, Fattaey A (1993) Inhibition of E2F-1 transactivation by direct binding of the retinoblastoma...

Ngày tải lên: 25/10/2013, 21:20

... of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of Ngoc Linh ginseng cell culture Materials and methods Induction of callus Fresh mountain ginseng roots ... Suspended cells of P vietnamensis were initiated through callus induction from the cultivated plant root [5] The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric ... suspension cultures of P vietnamensis for biomass and production of ginseng saponin (secondary metabolite) The final dry cell weight was increased from 5.4 to 10.3 g/L with an increase of initial sucrose...

Ngày tải lên: 12/02/2014, 10:20

... intracellular calcium level increased in OccWT-overexpressing cells, and that 2-APB inhibited the effects of OccWT Therefore, the increase in intracellular calcium may explain the induction of ... function of occludin, we reduced occludin expression in Huh7 cells by using occludin short hairpin RNA (shRNA) After determining the reduced expression level of occludin with immunoblotting (Fig ... facilitates the initiation of apoptosis [22] In addition, elevated calcium levels contribute to cell invasion [23,24] In contrast, in the case of occludin, an ER-mediated increase in intracellular calcium...

Ngày tải lên: 18/02/2014, 18:20

... Glutamine incorporation in Caco-2 proteins determining the relative uptake of glutamine; (b) by searching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into cellular ... addition, galectin-3 is suggested to be involved in cell cell and cell matrix interactions It is an intracellular and extracellular lectin, which interacts with intracellular glycoproteins, cell surface ... probably interacts with LI-cadherin by its carbohydrate recognition domain, on the cell surface of pancreatic carcinoma cells [51] Alpha-actinin 4, like annexin A2, is an F-actin cross-linking protein...

Ngày tải lên: 20/02/2014, 01:20

... Fedi, M Voutilainen Helsinki Institute of Physics, Helsinki, Finland S Czellar, J Họrkửnen, A Heikkinen, V Karimọki, R Kinnunen, M.J Kortelainen, T Lampộn, K Lassila-Perini, S Lehti, T Lindộn, P Luukka, ... density [34] in the calorimeter and tracker originating from additional inelastic pp interactions (pile-up) in the same bunch crossing as the DY interaction.The calculation of takes into account ... and energy densities are corrected using a bin-by-bin technique In the bin-by-bin technique, the correction factor is calculated by taking the bin-by-bin ratio of the particle level and detector...

Ngày tải lên: 07/03/2014, 17:20

... distribution of VBARP is independent of the cycling stage of the cells Identification of biological function(s) of VBARP using a siRNA assay To examine the role of endogenous VBARP in the regulation of ... implicated in cell survival and may play a role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase (RTK) signaling, independent of MAP kinase (MAPK) ... by either functioning downstream of MAPK or by defining a new pathway of RTK signaling [8] RTKs play important roles in cell signaling during cell proliferation, apoptosis, and cell survival upon...

Ngày tải lên: 07/03/2014, 21:20

... the concentrations of CSP existing in vivo, the unspecic inhibition of protein expression observed in the living cell after a cold shock can be explained by a direct binding of CSP to RNA and ... (d) the increased activity of proteolytic enzymes cleaving the target protein Other effects which can occur in whole cells (e.g the induction of new proteins interfering with the protein expression) ... of 230 lM TmCSP in buffer A; lane E: addition of 420 lM BcCSP in buffer B; lane F: addition of 800 lM BsCSP in buffer B Fig Inuence of CSPs on the in vitro translation of CAT Activity level of...

Ngày tải lên: 07/03/2014, 21:20

... identification of certain GRs needs to be made more accurately We intend to annotate more in- domain training data for GR labeling, and we are currently investigating the use of other applicable GR parsing ... Annual Meeting of the Association for Computational Linguistics (pp 128-135) Dekang Lin 1998 Dependency-based evaluation of MINIPAR In Proceedings of the Workshop on the Evaluation of Parsing Systems ... (A) contains 20 transcripts of children of ages ranging between two and three The second set (B) contains 25 transcripts of children of ages ranging between eight and nine Each transcript in set...

Ngày tải lên: 08/03/2014, 04:22

... dendritic cells, independently of CD81 expression [51,52] The limited involvement of CD81 in the binding process was further illustrated by the poor binding inhibition achieved in the presence of mAbs ... Regeard et al In vitro HCV infection models mutations Furthermore, it should be kept in mind that, in this system, hundreds of RNA copies per cell are present, in contrast to 5–50 copies in infected ... culture of infected hepatocytes To succeed in obtaining HCV infection in primary human hepatocytes, an existing model of hepatitis B virus infection was used to determine optimal infection conditions...

Ngày tải lên: 16/03/2014, 05:20

... adenocarcinoma and ovarian carcinoma develop [13,14] DPIV overexpression in melanoma and non-small cell lung carcinoma cell lines inhibits the processes of tumor progression, including anchorage-independent ... DPIV-transfected non-small cell lung carcinoma cells have shown inhibition of cell migration, increased apoptosis, inhibition of anchorage-independent growth and suppression of tumor growth in nude mice [16] ... b-catenin can in uence cell adhesion and apoptosis [23,28] DDR1 is an integrin-independent cell adhesion molecule DPIV reduces cell adhesion by dephosphorylating p38 MAP kinase and b1-integrin [29],...

Ngày tải lên: 23/03/2014, 11:20

... pricing services? Funds often obtain prices from brokers or pricing services to determine the fair value of their holdings of financial instruments When assessing the appropriateness of using ... hierarchy, the inclusion of a lower-level input in a fund’s measurement may indicate that the input is significant because of the fund’s decision to include it in the overall measurement of fair value ... participants? In determining the inputs to valuation models that comply with IAS 39, market information is considered whenever possible Typical inputs to valuation models for financial instruments include...

Ngày tải lên: 23/03/2014, 11:20

... The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric acid (IBA), 0.1 mg/L of kinetin and 30 g/L sucrose The pH was adjusted to 5.8 before autoclaving Cells ... obtained when 0.5 strength level of NH4NO3 from the culture medium Table Biomass growth of ginseng cell was affected by concentration of macro elements in the MS medium Cultures were maintained in ... Concentration of macro element NH4NO3 KNO3 Determination and analyses Extraction and determination of ginsenoside production were determined as reported previously [8,9] MgSO4 Determination of cell growth...

Ngày tải lên: 28/03/2014, 10:20

... What is recognised in the reporting period? Proit or loss Interest calculated using the EIR method in the currency of denomination of the instrument Interest income is recorded in the functional ... Investment Funds How you apply the EIR method to calculate interest income from a loating rate instrument? The EIR of a loating rate instrument changes as a result of periodic re-estimation of ... estimated inclusive of such credit losses Only in rare cases when it is not possible to determine estimated cash lows or the expected life of a inancial instrument or a group of similar inancial instruments,...

Ngày tải lên: 30/03/2014, 03:20

... Center, Purdue University, West Lafayette, Indiana School of Mechanical Engineering, University of Science and Technology Beijing, Beijing, China Department of Physics and Birck Nanotechnology Center, ... induces a heating effect, shifting the Raman peak due to bond softening and thermal expansion Temperature information can be obtained by measuring this heat-induced Raman peak shift Using a simple ... mainly because the single-layer graphene absorbs only about 3% of the total laser power, yielding large relative uncertainties in the final results even with a very small uncertainty in determining...

Ngày tải lên: 06/05/2014, 08:54

... of pipe butt welds The procedure involved uniformly heating the cluster up to 600°C, holding for a period of hour and cooling back to room temperature Measurements of the movement of the lacing ... residual stress measurements in a mining boom cluster before and after PWHT Experience with the use of incremental hole drilling technique for residual stresses measurements in the clusters is ... scans (1 and 4) This can be explained by rapid cooling in the last weld bead, particularly for scan Using understanding gained in work on neutron beam measurement of multi-bead welds18 it is probable...

Ngày tải lên: 17/05/2014, 15:28

... NI, Husain SR, Kreitman RJ, Haas GP, Pastan I, Debinski W: Targeting of interleukin-13 receptor on human renal cell carcinoma cells by a recombinant chimeric protein composed of interleukin-13 ... counterstained by DAPI positive pancreatic cancer cell lines, but not in IL13Ra2-negative cell lines In contrast, histones in IL13Ra2-negative pancreatic cell lines and normal cell lines were ... metalloproteinases (MMPs) expression in IL-13Ra2 expressing pancreatic cancer cell lines [28], we investigated the impact of IL-13Ra2 upregulation by HDAC inhibitors by examining IL-13 induced MMPs...

Ngày tải lên: 18/06/2014, 19:20