measurement of dna damage in spermatozoa by tunel assay

... death Potential DNA strand break induction and crosslinking was investigated by immunostaining of damage markers as well as automated fluorometric analysis of DNA unwinding Changes in nuclear morphology ... mitomycin C, strong crosslinking prevents drop in signal intensity by irradiation d: Time course of DNA breaks and crosslink detection by FADU after application of 10 μM Tc52 to HeLa cells for the indicated ... to induce cell death by microtubule fragmentation during mitosis [50] Indeed, visualization of tubulin and F-actin indicated that Tc52 induced small alterations in the tubulin network, displaying

Ngày tải lên: 21/09/2020, 01:57

... role of DNAM-1, the assay was performed in parallel treating NK cells with blocking anti-DNAM-1 antibody Results are representative of one out of three independent experiments B) The MFI of CD107 ... and killing by promoting DNAM-1 recognition Molecular mechanisms involved in PVR/CD155 up-regulation by NO One of the most studied mechanisms involved in physiological pathways regulated by NO ... genotoxic chemotherapeutics, inhibitors of the HSP-90 protein or of the GSK3 kinase, can increase the expression of several NK activating ligands on MM cells, via induction of specific regulatory transcriptional

Ngày tải lên: 30/09/2020, 13:35

... curves of the three clusters in terms of the AE hits’ energy The energy of AE signals in cluster increase stably at the beginning of the test The AE activities are very energetic in the regime of ... and damage evolution during cycling But in all fatigue tests, even though the areas identified in relation to the damage corresponding recoveries are uncertain and make assigning a signal to damage ... of AE are powerful means for identification of damage phenomena and monitoring of their evolution AE [6] is a transient wave resulting from the sudden release of stored energy during damage In

Ngày tải lên: 01/11/2022, 09:06

... member, and in this way the fractional measurement of things came about. There are three general divisions of measurements and these are (1) the mea- surement of time; (2) the measurement of space ... column of the checks drawn,... used before whole numbers they were certainly used concurrently with them In fact the idea of a whole number is made clearer to the mind by thinking of ... CUTS IN FIGURES CHAPTER I WHAT ARITHMETIC IS The Origin of Calculation. Ratios and Proportions. The Origin of Counting and Figures. Practical Applications of Arithmetic. Other Signs Used in Arithmetic.

Ngày tải lên: 28/06/2014, 19:20

... amitinus (23%) (Table 3).  e magnitude of colonization was approaching the carrying capacity of trees. In the case of trees struck by lightning in the area with an endemic population of I. ... Merocoenoses of cambioxylophagous insects in wind-felled trees, breaks and fragments  e trees felled by the wind in autumn were checked for the presence of cambioxylophagous in- sects in next spring ... 2009).  e structure of merocoenoses of cambiopha- gous insects in lying (wind-felled) trees diff ers from that of standing trees (in endemic situation) in the high attack by I. typographus and

Ngày tải lên: 07/08/2014, 10:21

... elements in the teeth have been previously demonstrated. 1 Trace elements can be ingested by humans via different routes, including ingestion in the food and water or by deliberate consumption of ... Other sources include the use of Cd for industrial applications, such as in the production of pigments, stabilisers, and alloys. In addition, Cd is present in trace amounts in certain foods, such ... availability of foods and food choices, which are often influenced by social and cultural practices, may be important factors in determining which trace elements are ingested by humans and

Ngày tải lên: 07/08/2014, 14:20

... Another major kinase activated by DNA damage is DNA- PK, which is activated by binding to the damaged sites on DNA [18]. The binding of DNA- PK to DNA was evaluated by DNA- PK chromatin immunoprecipitation ... mechanisms of radiosensitization are known, including redox modulators [21], inhibitors of DNA dam- DNA damage induced by DCQ+IR in EMT-6 cellsFigure 2 DNA damage induced by DCQ+IR in EMT-6 cells. ... arrest induced by DCQ and the S-phase accu- mulation induced by IR appear together in combination treatments. Despite our intriguing findings that the com- bination treatment DCQ+IR induces DNA damage,

Ngày tải lên: 09/08/2014, 10:20

... one of those described by C HOURROUT & Q UILLET (1982) : Twenty five min. after the beginning of incubation, a part of each group was transferred for twenty min. in ... permitted by artificial insemiTSKOVICH nation in Tilapia : Induced diploid gynogenesis, production of all-triploid populations and intergencric hybridization Proceedings of the International ... acid ; after rinsing and drying, the slides were stained in 4 p. 100 Giemsa for 10 minutes. E. Biochemical studies Starch gel electrophoresis of muscle proteins was performed

Ngày tải lên: 09/08/2014, 22:23

... 6. Results Inflammation of the skin by curdlan intradermal injection Skin inflammatory response induced by intradermaly injected curdlan (0.5 mg/50 µl), was investigated first by using two parameters, ... application of essential oils on the accumulation of neutrophils in inflammatory sites in skin of mice. Methods: Inflammation with accumulation of inflammatory cells was induced by injection of curdlan, ... inflammation by intradermal curdlan injectionFigure 2 Effects of intraperitoneal injection of geranium oil on the inflammation by intradermal curdlan injection. Geranium oil was injected immediately

Ngày tải lên: 11/08/2014, 08:21

... at a single condition: an accurate measurement of changes in FRC either by CT or by the gas dilution technique. Again, the recent possibility offered by recent ventilators of measuring FRC by the ... influenced by aeration changes occurring during the initial part of the inflation P–V curve performed in ZEEP conditions. Competing interests The authors declare that they have no competing interests. ... sels). In such a situation, the region of interest manually delineated on the ZEEP CT section was manually delineated on the two corresponding CT sections obtained in PEEP con- ditions. In each of

Ngày tải lên: 12/08/2014, 23:24

... leucine-rich acidic nuclear protein LH luteinizing hormone LHX3 LIM homoeodomain 3 LHX4 LIM homoedomain 4 LIM Lin11,Isl-1, Mec-3 MBD methyl-CpG binding domain protein MBP methyl CpG binding ... brac/pox virus and zinc finger domain CBP 3’-5’-cyclic adenosine monophosphate response binding element -binding protein CpG cytosine-phosphate bond-guanine CPHD combined pituitary hormone ... effects of DNA silencing involve alterations in protein -DNA interactions, and thus implicated histones and other transcription factors in the mechanism Studies examining the interactions

Ngày tải lên: 24/08/2014, 09:47

... species Economists define the value of change in terms of How much of something else an individual is willing to give up to get this change/or How much they would accept in order to permit the ... may measure different things Considering the needs of the users of valuation studies And the needs of the public The cost of carrying out a valuation study Components of a CV questionnaire  ... using a questionnaire: Zone of origin and other demographic/attitudinal information Frequency of visits to the site Frequency of visits to substitute sites Trip information: length, nights

Ngày tải lên: 03/02/2015, 15:50

... DNA vaccine and Application of DNA vaccine in “Fisheries” Photo Album by Activated User Photo Album by Activated User  DNA vaccine is DNA sequence used as a vaccine  This DNA sequence ... the virus, denoted by the G protein, that is the immunetic protein of the virus Insect G-gene into DNA plasmid... plasmid form Recombinant DNA Inject DNA vaccine into Fish G-gene construct ... protein of pathogen  As this DNA inserted into cells it is translated to form antigenic protein. As this protein is foreign to cells, so immune response raised against this protein.  In

Ngày tải lên: 18/05/2015, 18:30

... University of Baghdad, Iraq in 1997. He obtained Msc degree in Physics Sciences from University of Technology in 1989 , He work as Director General of the Department of renewable energies in Ministry Of ... Bsc in Environmental Engineering in 2002 from Al-Mustansiriya University. He work in the Ministry of Science and Technology in implementation of treatment station of water filter and examinations ... units on the wind erosion for finding dust storm sources in regions of western parts of Iran [7]. The researchers in reference [8] have calculated the dust storm velocity by determining the front

Ngày tải lên: 09/09/2015, 10:17

... It is divided into two parts. In the first part, the binding between a small GTPases protein vii Cdc42, and one of its effectors, IQGAP1, is investigated. The Kd of the binding in cell culture ... these factors influence the quality of the determined Kd. Major findings include quantitating the fraction of non-fluorescent red fluorescent proteins (mRFP and mCherry) and investigating the relationship ... UNIVERSITY OF SINGAPORE 2011 This work was performed in the Department of Chemistry at the National University of Singapore under the supervision of Associate Professor Thorsten Wohland, and in the Institute

Ngày tải lên: 10/09/2015, 08:38

... FINDINGS AND IMPLICATIONS FOR TEACHING AND LEARNING COHESION This chapter aims at summarizing the major findings of cohesive devices used in the third debate by Barack Obama and Mitt Romney in ... auto industry, not rewarding companies that are shipping jobs overseas; making sure that we've got the best education system in the world, including retraining our workers for the jobs of tomorrow. ... phrase “bringing manufacturing back to our shores”, “creating jobs”, “got the best education system” are stuffs of rebuilding America. Thus, “rebuilding America” is the super-ordinates of these

Ngày tải lên: 13/09/2015, 22:15

... 1.12.3 amh: a candidate gene inhibiting the expression of aromatase in zebrafish. .. inducing and maintaining ovarian development in fish (Yamamoto, 1969) Exposing zebrafish to E2 at 100ng/l ... of the human Y chromosome (Sinclair et al., 1990) The function of SRY as a sex determining 3 gene was proven by the finding of SRY in XX males (Palmer et al., 1989) and mutation of ... by providing a detailed view of gonadal differentiation in males, and establishing a method to obtain samples undergoing this key process by. .. thesis, the variety of sex determination

Ngày tải lên: 14/09/2015, 09:39

... of DNA under various conditions One of the most promising applications is the investigation of the effects of binding and non-binding proteins on the properties of confined ... CONFORMATION OF DNA CONFINED IN A NANOCHANNEL OR ABSORBED AT AN INTERFACE ZHANG CE NATIONAL UNIVERSITY OF SINGAPORE 2008 ON THE CONFORMATION OF DNA CONFINED IN A NANOCHANNEL OR ABSORBED AT AN INTERFACE ... the effects of confinement, charge, and self-avoidance. It was found that the elongation of the DNA molecules with decreasing ionic strength can be interpreted in terms of an increase of the persistence

Ngày tải lên: 14/09/2015, 14:01

... president of the National Academy of Engineering The Institute of Medicine was established in 1970 by the National Academy of Sciences to secure the services of eminent members of appropriate professions ... Institute; Professor of Biochemistry and Molecular Biology and Medical Genetics, University of Wisconsin DAN MOTE, Jr (Ex-Officio), President and Glenn Martin Institute Professor of Engineering, ... policies determined by the Academy, the Council has become the principal operating agency of both the National Academy of Sciences and the National Academy of Engineering in providing services

Ngày tải lên: 04/04/2017, 08:51

... Labeling DNA Damage with Terminal Transferase I LABELING DNA BREAKS USING TERMINAL TRANSFERASE (TUNEL ASSAY) Walker et al Labeling DNA Damage with Terminal Transferase Labeling DNA Damage ... understanding of human disease One of the most widely used methods for detecting DNA damage in situ is TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining (1) TUNEL staining was initially ... digoxigenin-conjugated dUTP detected by staining with a FITC-conjugated anti-digoxigenin antibody or biotin-conjugated dUTP detected by staining with FITC-conjugated streptavidin Labeling DNA Damage...

Ngày tải lên: 11/04/2014, 09:47