mdm2 ring finger protein and regulator of p53

... recognize different epitopes of the p53 product, were used for the detection of overexpression of mutant p53 protein, and MIB-1 (monoclonal antibody to Ki67 antigen of mouse origin, : 50 dilution, ... antibodies against the p53 protein and MIB-1 on formalinfixed, paraffin-embedded tissue specimens from initial tumors PAb240 and PAb 421 (monoclonal antibody to p53 protein of mouse origin, 1:50 ... place of the primary antibody Positive immunostaining for p53 protein with PAb240 antibody was found in case (25%) The proportion of benign and malignant lesions stained for p53 are 20% and 30%...

Ngày tải lên: 07/08/2014, 17:22

... presence and absence of p53 4.6 A protein expression and interaction network of proteins, involved 80 in cellular growth, proliferation and cell cycle, in the absence of p53 under normoxia and hypoxia ... deciphering the activation and function of this gene in cell at both normal and neoplastic states Regulation of p53 level in cell is mainly under the control of Mdm2 while activation of p53 involves ... funcitons and diseases identified by IPA 76 4.4 Tabulation of common proteins regulated in cells under 81 hypoxia in the presence and absence of p53 as well as in the absence of p53 under hypoxia and...

Ngày tải lên: 16/09/2015, 15:43

... transplatin and incubated with p53 In accordance with the results published earlier [13], no reduction of the intensity of the band corres- DNA recognition by p53 protein A B Fig Binding of active p53 protein ... adducts of BBR3464 and cisplatin produce differential responses to p53 protein activation and recognition It is therefore of great interest to examine whether DNA adducts of cytostatic analogues of ... 1,3intrastrand CL of trans-Pt-HMP, 12% of the 1,3intrastrand CLs were transformed into the interstrand CLs Importantly, the yields of these rearrangement reactions involving the 1,3-intrastrand CLs of...

Ngày tải lên: 16/03/2014, 14:20

... phosphorylation of recombinant p53 with the phosphorylation of S15A and S37A substituted p53 (p53S15A, p53S37A) in the presence of ssM13 and linearized double-stranded plasmid DNA (Fig 1) In the presence of ... with 0.2 lM of p53 peptides or 100 ng of recombinant p53 at 30 °C for 15 in the presence of 4.2 nM of [32P]ATP[cP] (3000 CiÆmmol)1), 10 ng of DNA and 10 units of DNA-PK in 20 lL of reaction buffer ... contrary, preincubation of the DNA-PK for 10 in the presence of 50 lM ATP and ssM13 prior to addition of p53 and [32P]ATP[cP], led to phosphorylation of both p53wt and p53S15A/S37A by 80% (Fig...

Ngày tải lên: 30/03/2014, 15:20

... Figure 2, p53, p21/WAF1, and MDM2 were mainly located in the nuclei of the cancer cells and highly expressed in the HCC Figure Expression of p53, p21/WAF1 and MDM2 in HCC by IHC Expression of p53, ... SPSS 13.0 software for Windows http://www.translational-medicine.com/content/7/1/110 Figure ern blot1 Expression of p53, p21/WAF1 and MDM2 in HCC by WestExpression of p53, p21/WAF1 and MDM2 in HCC ... proportions of p53, p21/WAF1, and MDM2 expression in HCC tissues were 70.7% (128/181), 33.1% (60/181), and 52.5% (95/181), respectively Positive proportions of p53, p21/WAF1, and MDM2 expression...

Ngày tải lên: 18/06/2014, 15:20

... 33, and 33 variFigure ants Comparing p53 protein levels and p53 transcriptional activity in cells expressing E6-GFP from HPV types 18, 33, and 33 variants Panel A: Western blot analysis of p53 and ... interactions of viral E6 with key cellular regulatory proteins Results Comparison of p53 and MAGI-3 degradation by high risk HPV-E6 We have previously demonstrated that HPV-18 E6 and E6GFP fusion proteins ... http://www.virologyj.com/content/5/1/67 anti -p53 anti-GFP Figure HPV-18 blot analysis of E6-GFP and HPV-16 E6 WesternE6-GFP, HPV-3 3p53 degradation in the presence of Western blot analysis of p53 degradation in the presence of HPV-18...

Ngày tải lên: 20/06/2014, 01:20

... 6.1.2 Comparison of p53 from lung of P annectens with p53, p63 and p73 of other animals……….…………………………… 199 6.1.3 Changes in mRNA expression of p53 in the lung during three phases of aestivation………………………………………… ... sequence the p53 from the lung of P annectens and to determine its mRNA expression levels in the lung and kidney during three phases of aestivation The cDNA sequence of p53 from the lung of P annectens ... expression of p53 in the lung and kidney of P annectens during the three phases of aestivation It is hypothesized that p53 expression would increase in the kidney during the induction and arousal...

Ngày tải lên: 09/09/2015, 17:55

... Desensitization of G protein Signaling 1.2.3.1 The discovery of Regulator of G protein signaling (RGS) Studies of the desensitization of G -protein- coupled signal transduction have led to the discovery of ... G proteins in yeast…………………………………………… 22 1.2.2.3 G proteins in mammals………………………………………… 23 1.2.3 Desensitization of G protein Signaling…………………………… …… 24 1.2.3.1 The discovery of Regulator of G -protein ... interacted with and individually accelerated the GTPase activity of each of the three Gα subunits (MagA, MagB and MagC) in Magnaporthe Thus, as a unique and multifunctional regulator of G protein signaling,...

Ngày tải lên: 14/09/2015, 09:13

Ngày tải lên: 14/09/2015, 09:24

Ngày tải lên: 14/09/2015, 09:39

Ngày tải lên: 14/09/2015, 09:53

... only one isoform of E1 and multiple isoforms of E2s and E3s The multitude of E2 and E3 enzymes allows elaboration of ubiquitination pathway in vivo and therefore ensures the specific and regulated ... Institute of Molecular and Cellular Biology (IMCB) for their close monitoring on the progress of my PhD project and offering of constructive and helpful suggestions during the yearly committee ... phosphorylates p53, leading to the inactivation of p53 transcriptional activity and enhanced MDM2- mediated p53 degradation [18] A fine-tuned balance of this mutual suppression mechanism between Aurora-A and...

Ngày tải lên: 14/09/2015, 22:15

... – ARF LIKE PROTEIN (ARL1) IS A NEW REGULATOR OF GOLGI STRUCTURE AND FUNCTION LU LEI (B.SC.) UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... of guanine nucleotide binding mutants of Arl1 vi 4.5 Expression of the GDP restricted form of Arl1 (Arl1T31N) causes disassembly of the Golgi apparatus 4.6 Expression of GTP restricted form of ... Sec1/Munc18 family proteins, tethering molecules, Raslike Rab and ARF small GTPases (reviewed by Hong, 1996) The importance of small GTPases, especially those of the ARF and Rab families, in protein trafficking...

Ngày tải lên: 17/09/2015, 17:20

... adiponectin and the C-terminus of GLP-1-A Compared with native GLP-1, the fusion protein has a modified site and larger molecular size, and may circumvent the rapid inactivation and renal clearance of ... part of fusion protein or both of them In other words, we need to know whether each part of the fusion protein play glucose-lowering effect separately We also need to know whether the half-life of ... 2011, Purification and refolding of N-terminal His-tagged gAd-GLP-1-A fusion protein The fusion protein was purified by High-Affinity Ni-IDA Resin After filtering, the fusion protein was bound...

Ngày tải lên: 25/10/2012, 11:10

... first pass of signal-noise ratio of and a minimum peak threshold of 20% of all spectra This process was completed with a second pass of peak selection at 0.2% of the mass window, and the estimated ... mixtures of proteins Captured proteins are then analyzed by TOF-MS, generating a spectral map depicting approximations of the molecular weight (m/z) and relative concentration (intensity) of each protein ... Identification of Biomarker Pattern and Construction of Diagnostic Model The decision tree could differentiate samples of CRC and healthy controls Here, we used the biomarker wizard function of the ProteinChip...

Ngày tải lên: 25/10/2012, 11:18

... evidence for an interaction between NS1 and PARP Since PARP is active at the site of single strand DNA breaks, and single-strand nicking is a known activity of NS1 protein, it is likely that NS1 is ... nonstructural proteins of MVM and H-1 parvovirus attach to the viral genome Covalent attachment of NS1 to cellular DNA was investigated in this study using denaturing SDS-PAGE and autoradiography ... in a covalent manner, and that both DNA-helix distorting and single strand nick forms of DNA damage are important pathways to apoptosis upon expression of NS1 Materials and Methods Transfection...

Ngày tải lên: 25/10/2012, 11:18

... Z., and Pomeranz, Y 1994 Role and contribution of starch and protein contents and quality to texture profile analysis of oriental noodles Cereal Chem 71:315-320 Batey, I L., Curtin, B M., and ... (negatively) with total texture score and its two components independently of any effect of protein quality (farinograph stability) Effect of Protein Content and Quality Protein content was not correlated ... and RVA parameters, with and without inactivation of Germination of Grain Samples Grain samples were germinated as previously reported (Crosbie and Lambe 1993) Preparation of Flours Samples of...

Ngày tải lên: 16/12/2012, 15:25

... series of GST-fused deletions of the CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the ... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned ... of EGFP, KIND2 or KIND2-1 (Fig 5B) These results suggest that KIND2 and KIND2-1, overexpressed in neurons, act as dominant-negative regulators of v-KIND and suppress the negative regulation of...

Ngày tải lên: 14/02/2014, 19:20

... glycinin and b-conglycinin are synthesized and translocated into the ER lumen during the maturation stage of embryogenesis Therefore, we next measured the mRNA and protein levels of GmPDIL-3a and ... PDI family proteins of yeast and animals [9–12], but this is the first report to confirm expression of such proteins in the ER of plants A CXXS motif and a CXXC motif in the N-terminal and C-terminal ... ER proteins, or regulation of ER calcium channel activity [9–12] In plants, a set of 22 orthologs of known PDI family proteins was discovered by a genome-wide search of Arabidopsis thaliana, and...

Ngày tải lên: 18/02/2014, 11:20

... C275S Q192R D208E T256A N263D V272A A276T R283C p53R2 ⁄ NOXA p53R2 p53R2 p53R2 p53R2 p53R2 WAF1 WAF1 ⁄ MDM2 WAF1 WAF1 ⁄ MDM2 WAF1 ⁄ MDM2 WAF1 ⁄ MDM2 MDM2 82–128 131 143 97 56 223 32 2–12 11 1–18 ... mutations and NOXA and p53R2 slightly less so This is indicated by the average activity of mutants in the central domain of 26% for WAF1 and 34% for MDM2, 71% for NOXA and 61% for p53R2, and around ... advantage of p53 mutation analysis, and a unique feature of this database, is the availability of the residual activity of the majority of p53 missense mutants The biological activity of mutant p53...

Ngày tải lên: 18/02/2014, 11:20