mature gc frass pulsed bone marrow derived myeloid dcs
Báo cáo y học: "Abnormal spatial diffusion of Ca2+ in F508del-CFTR airway epithelial cells" pptx
... 5'AGTTTGTTGAGTAGCACTGCGTCT-3' 86 hlITPR NM_002223 NM_002224 5'-AAGTATTAATGTA GGCCCAAGACCTATT-3' 5'-GGAGGGCTTGC GGAGAA-3' 117 hlITPR 5'-AACCGCTACTC TGCCCAAAA-3' 5'-GCGATCTGCA CATCTATGCTG-3' 5'-GGGCTCTCG GTGCCTGA-3' ... β-actin-cDNA, the primers were 5'TGTGGATCGGCGGCTC-3' and 5'-ACTCCTGCTTGCTGCTGATCCAT-3' (900 nM for each primer) The probe taqman FAM used was 5'FAM-TGGCCTCGCTGTCCACCTTCCA-TAMRA3' (200 nM) The temperature ... AM at room temperature in buffer solution containing: (in mM) 130 NaCl, 5.4 KCl, 2.5 CaCl2, 0.8 MgCl2, 5.6 glucose, 10 Hepes, pH 7.4 (adjusted with Tris base) Cells were then washed and allowed...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
... several arginyl residues were involved in electrostatic interactions with the phosphdiester backbone of the hexanucleotide The role of four such arginyl residues (Arg48, Arg134, Arg213 and Arg258) ... incubated at 30 °C for h with the relevant RTA variant in 25 mM Tris/HCl (pH 7.6), 25 mM KCl, mM MgCl2, in a total volume of 20 lL Reactions were stopped by the addition of 100 lL of 2· Kirby buffer ... Biochem 271) R213D R213D Fig Electron density of RTA R213D in the vicinity of residue 213 The backbone and sidechains of the R213D substitution are shown as stereo images in thick ball and stick...
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
... activity of an antibody-heme complex J Am Chem Soc 112, 9414±9415 22 Keinan, E., Sinha, S.C., Sinha-Bagchi, A., Benory, E., Ghozi, M.C., Eshhar, Z & Green, B.S (1990) Towards antibodymediated metallo-porphyrin ... anti-porphyrin antibodies, Chem Lett., 953±956 24 Keinan, E., Benory, E., Sinha, S.C., Sinha-Bagchi, A., Eren, D., Eshhar, Z & Green, B.S (1992) Catalytic antibodies: circular dichroism and ultraviolet-visible...
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báo cáo hóa học: " The effect of hip abduction on the EMG activity of vastus medialis obliquus, vastus lateralis longus and vastus lateralis obliquus in healthy subjects" pot
... lower-extremity musculature in chronic symptomatic instability of the anterior cruciate ligament J Bone Joint Surg [Am] 1985, 67:1034-43 McConnell J: Management of patellofemoral problems Man Ther ... patellofemoral pain Clin Sports Med 1989, 8:197-202 Bevilaqua-Grossi D, Monteiro-Pedro V, Sousa GC, Silva Z, Bérzin F: Contribution to the anatomical study of the oblique portion of the vastus ... Lieb FJ, Perry J: Quadriceps function: an anatomical and mechanical study using amputated limbs J Bone Joint Surg [Am] 1968, 50:1535-48 Wilk KE, Davies GJ, Mangine RE, Malone TR: Patellofemoral...
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Investigations on the antiplasmodial activity of ferrocenyl chalcones
... drugs is to produce estrogenic actions in those tissues where such effects are beneficial (such as bone, brain, liver during postmenopausal hormone replacement) and to have either no activity or ... decreases with time due to the acquisition of drug resistance by the tumours Ferrocifens - compounds derived from the replacement of one of the phenyl rings of tamoxifen with ferrocene, was one of...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 3
... (2, 5, 211, 227, 228, 234) were found to be inhibitory (Table 6.1) Interestingly, the actives are derived from only two classes of ring B substituted chalcones, namely the hydroxylated chalcones...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 2
... hydroxylated chalcones) and PLS is carried out separately on each group A summary of the models derived for each class and their relevant statistical characteristics are given in Table 5.1 Table ... Therefore, 41 is considered together with the trimethoxychalcones in the PLS analysis The 1st model derived from PLS analysis of the trimethoxychalcones (including 41 and all 11 physicochemical descriptors) ... model (2), which explained 77% (r2 = 0.766) and predicted 38% (q2 = 0.377) of the activity was derived (Table 5.1) With 11 descriptors in model (2), it is highly probable that some descriptors...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 1
... inhibition of this enzyme by artemisinin may be anticipated since it shares a similar sesquiterpene backbone as thapsigargin, a known inhibitor of microsomal calcium ATPase As in the case of its general ... 2’,6’-dihydroxy-4’methoxychalcone which was active against promastigotes and amastigotes of L amazonensis 85 Several plant -derived chalcones are also found to be antileishmanial 86 1.3.3 Immunosuppressive activity The immunosuppressive ... is dependent on growth factors (like epidermal growth factor, fibroblast growth factor, platelet -derived growth factor), cytotoxicity may be due to interference with the availability of these factors...
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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... from Roche (Switzerland) The p12 DNA oligonucleotide (5Â-GTCTTTCTGCTC-3Â), the tC5U RNA oligonucleotide (5Â-CCCCCUCUCAAAAACAGGAGCA GAAAGACAAG-3Â) and the 12mer DNA oligonucleotide FEBS Journal ... RNase H catalytic cavity and the other close to the NNRTI binding pocket Because experimental data derived from testing the compounds on the isolated RNase H portion seem to suggest that the rst ... interact with the metal ions, we measured their visible spectrum in the absence or presence of mm MgCl2, observing that addition of the cation did not signicantly alter the alizarine derivatives...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... Tris/HCl (pH 7.5), mm MgCl2 and 0.5 mgÆmL)1 bovine serum albumin The mixture was heated for at 77 °C to stop the reaction and then dialysed against 50 mm Tris/HCl (pH 8.1) and mm MgCl2 11 12 13 14 ... complex The reaction mixture (10 lL) containing 50 mm Tris/HCl (pH 7.5), 20 mm 2-mercaptoethanol, mm MgCl2, mm ATP, 100 lgÆmL)1 bovine serum albumin and the MCM4/6/ complex (170 ng) was incubated at ... containing 20 mm Tris/HCl (pH 7.9), 3.3 mm 2-mercaptoethanol, 0.2 mgÆmL)1 bovine serum albumin, mm MgCl2, 0.25 mgÆmL)1 activated DNA, 100 lm each of dATP, dGTP and dTTP, 50 lm of dCTP, 111 kBq [a-32P]dCTP...
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Báo cáo khoa học: Effect of magnesium ions on the activity of the cytosolic NADH/cytochrome c electron transport system pptx
... addition of mM MgCl2, at 10 addition of lM cyto-c, and the reaction stopped at 15 (Mg-c); at addition of lM cyto-c, at 10 addition of mM MgCl2, and the reaction stopped at 15 (c-Mg); mM MgCl2 present ... immediately or at 10 (m); at min, mM MgCl2 and the reaction stopped at 10 (m-Mg); at zero time, mitochondria added to the medium already containing mM MgCl2 and the reaction stopped at either ... 166 lM NAD+(N); 45 IU alcohol dehydrogenase (A); mM MgCl2 (Mg); 1.6 lM FCCP (F); mM potassium cyanide (CN); 30 lM TMPD (T) In trace e, mM MgCl2 was already present in the incubation medium before...
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Báo cáo khoa học: The ‘pair of sugar tongs’ site on the non-catalytic domain C of barley a-amylase participates in substrate binding and activity potx
... the sense strand, and 5Â-TTTGGTACCTCAGTTCTTCTCCCAGA CGGCGTA-3Â as antisense primer Mutant cDNA was amplied using 5Â-TTTGAATTCCATGGGGAAGAACG GCAGC-3Â as sense orientation primer and a puried megaprimer ... the mega-primer method [47] using for S378P, 5Â-GATCGGGCCCAGGTACGACGTC GG-3Â; S378T, 5Â-GATCGGGACCAGGTACGACGTCG G-3Â; Y380A, 5Â-GATCGGGTCCAGGGCCGACGTC -GG-3Â; Y380M, 5Â-GATCGGGTCCAGGATGGACGT CGG-3Â; ... of the active site cleft Earlier, signicant deviation was found in this region between the backbone conformation of AMY1 and AMY2 [36] Thus Ca of Gly214 shifted 0.8, 1.2, and 1.4 A relative...
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Báo cáo khoa học: On the mechanisms of bananin activity against severe acute respiratory syndrome coronavirus pptx
... hydrophilic pocket present on the helicase surface This surface pocket, formed by N257 and I258 backbone atoms together with S259, D260 and E261 side chains in the wild-type helicase ˚ (Fig 2), reduces ... BJ, Zhou J, Watt RM, Jiang JQ, Wong KL, Lin YP, Lu LY, He ML, Kung HF et al (2005) The adamantane -derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus ... Kesel AJ (2003) A system of protein target sequences for anti-RNA-viral chemotherapy by a vitamin B 6derived zinc-chelating trioxa-adamantane-triol Bioorg Med Chem 11, 4599–4613 16 Davies WL, Grunert...
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Báo cáo khoa học: The two IQ-motifs and Ca2+/calmodulin regulate the rat myosin 1d ATPase activity pptx
... the two primer pairs 5Â-GGCAAACTTGATGATGAGCG CTGC-3Â (forward 1) 5Â-CAGAGCTGCCTTGACGAGCATCTG-3Â (reverse 1) and 5Â-CAGATGCTCGTCAA GGCAGCTCTG-3Â (forward 2) 5Â-ATTCCAGCACACT GGTCACTT-3Â (reverse ... (reverse 2) To obtain Myo1dDLV-IQ2FLAG, the two primer pairs forward 5Â-CAGAGC TGCCTTCATCTGGGCGCG-3Â (reverse 1Â) and 5Â-ATT CCAGCACACTGGTCACTT-3Â (forward 2Â) reverse were used, respectively After ... measured at 37 C in a solution containing 24 lM F-actin, mM ATP, 30 mM KCl, 10 mM Hepes pH 7.4, mM MgCl2, mM EGTA, mM 2-mercaptoethanol and mM NaN3 To adjust free Ca2+ concentrations (0158 lM), corresponding...
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Báo cáo khoa học: Effects of a tryptophanyl substitution on the structure and antimicrobial activity of C-terminally truncated gaegurin 4 doc
... Fig Solution structures of GGN4 analogues Backbone atoms (N, Ca, and C¢) of the finally refined 20 structures were superimposed, by matching the backbone atoms in the helical region, for D24)37 ... followed by sequential assignments through the NOE connectivities [15,17,20] Distance restraints, backbone dihedral angle restraints, and hydrogen bond restraints were obtained and used for the structure ... micelles at pH 4.0, with a 200-ms mixing time The suppression of the water signal was achieved by the pulsed field gradient method All NMR spectra were processed and analyzed using the NMRPIPE/NMRDRAW...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008