matrix assisted laser desorption ionization time of flight ms

... data sets Iterative cycles of refinement and manual fitting were carried out using programs CNS [24] and O [25] To monitor the progress of the refinement, a total of 10% of the reflections were set ... evaluated with PROCHECK software [27] Comparison of family 11 xylanases The coordinates of 10 different family 11 xylanases were available in PDB First, the Ca atoms of all xylanase models were ... GNPGNP seems to be a part of the C-terminal tail of the full-length NFX with total 301 residues The amino acids of the C-terminal tail were sticking out from the model, but if the C-terminus of NFX...

Ngày tải lên: 20/02/2014, 23:20

... is a registered trademark of Computers and Structures, Inc SAP2000 is a registered trademark of Computers and Structures, Inc Windows is a registered trademark of Microsoft Corporation Adobe and ... Acrobat are registered trademarks of Adobe Systems Incorporated DISCLAIMER CONSIDERABLE TIME, EFFORT AND EXPENSE HAVE GONE INTO THE DEVELOPMENT AND DOCUMENTATION OF SAP2000 THE PROGRAM HAS BEEN ... screen During the course of this tutorial, we will explore many of the basic features of SAP2000 Prepare to spend at least one hour going through this example, and if at any time you need to stop,...

Ngày tải lên: 06/09/2012, 15:56

... unmatched in terms of ease -of- use and productivity Creation and modification of the model, execution of the analysis, and checking and optimization of the design, and production of the output are ... user-friendly release of the SAP series of computer programs When initially released in 1996, SAP2000 was the first version of SAP to be completely integrated within Microsoft Windows It features ... Groups A group is a named collection of objects It may contain any number of objects of any number of types Groups have many uses, including: Quick selection of objects for editing and assigning...

Ngày tải lên: 01/04/2014, 03:20

... factor of 18.8% and an Rfree of 22.3% with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2) The resulting model is depicted in Fig The Ca backbones of the ... TVA-II group As the polypeptide superposition of Fig places the three catalytic residues of CDase (Fig 1) within less than ˚ A of the positions of those of TVA-II, and as the active centers closely ... removal of Ca-I was shown to promote proteolysis [43,44] Ca-I is co-ordinated by the side chains of Asp280 and Ser222 at the beginning and end of domain B as well as by the main-chain oxygens of Tyr315...

Ngày tải lên: 08/03/2014, 02:20

... harvested after 24 h of culture on 2% (w/v) glucose, by using the method of Raeder & Broda [33] Amplification of a DNA fragment encoding a portion of the catalytic domain of T emersonii cel7 was ... structure solution of the native enzyme Family contains both CBHs and endoglucanases The structure of CBHs is distinguished from that of endoglucanases by the presence of loops of polypeptide chain ... Cellobiose is the predominant product of hydrolysis and increases in concentration as the reaction proceeds [21] The exo-loop of 1CEL (amino acids 243–256) forms the roof of the active site tunnel at...

Ngày tải lên: 23/03/2014, 13:20

... 2002 3D modelling of Neurospora H+-ATPase (Eur J Biochem 269) 5255 of internal cavities capable of containing water molecules in the homologous Rossman fold of the soluble members of the HAD superfamily ... middle of the a/b Rossman fold, down to the Fig Position of internal polar cavities in the Rossman fold of PMA1_NEUCR and two other soluble members of the HAD superfamily (A) Phosphorylation site of ... from the sequence of PMA1_NEUCR and those assigned in the equivalent regions of the ATC1_RABIT template (Fig 1) The C-terminal domain of both enzymes displayed a much lower level of sequence similarity,...

Ngày tải lên: 23/03/2014, 21:20

... (full-width at half maximum) of tens of nanometers, and its peak intensity can be orders of magnitude higher than a circularly-shaped aperture with a similar level of field confinement [3] Bowtie ... argon-ion laser (λ = 457 nm) A halfwave plate and a polarizer are used to control the polarization of the incident laser beam Because the light source is fixed with respect to the NSOM tip instead of ... The aperture has a size of 260 nm × 260 nm and has a gap of 62 nm (B) SEM image of the NSOM probe The hole on the tip is around 150 nm in diameter (C) Simulation model of bowtie nanoantenna Its...

Ngày tải lên: 06/05/2014, 08:54

... (full-width at half maximum) of tens of nanometers, and its peak intensity can be orders of magnitude higher than a circularly-shaped aperture with a similar level of field confinement [3] Bowtie ... argon-ion laser (λ = 457 nm) A halfwave plate and a polarizer are used to control the polarization of the incident laser beam Because the light source is fixed with respect to the NSOM tip instead of ... The aperture has a size of 260 nm × 260 nm and has a gap of 62 nm (B) SEM image of the NSOM probe The hole on the tip is around 150 nm in diameter (C) Simulation model of bowtie nanoantenna Its...

Ngày tải lên: 06/05/2014, 08:58

... area |c| of the polyomino c is the number of its unit squares We denote by Cn the set of all the polyominoes of area n The perimeter P (c) of a polyomino c is the number of unit edges of the dual ... the integer n is of the form l(l + ) or l(l + ) − figure 2.16: two elements of M23 Conversely, we will examine for these particular values of n the possible actions of the sequence of transformations ... decomposition of l1 l2 Any polyomino obtained by removing successively m < k corners from l1 × l2 is minimal Proof The removal of a corner cannot increase the perimeter of a polyomino The perimeter of...

Ngày tải lên: 07/08/2014, 06:20

... mixture of chloroform and methanol (2:1 [v/v]) After extraction, 0.2 volumes of 0.9% NaCl solution were added and the chloroform-containing phase was collected After evaporation of the chloroform ... that is considered to correspond to a cluster of quantum dots Figure 3-D model of the nucleus of the same MCF-7 cell as in Fig 3-D model of the nucleus of the same MCF-7 cell as in Fig The translucent ... (page number not for citation purposes) Journal of Nanobiotechnology 2006, 4:10 Figure 3-D model of the nucleus of a PC12 cell 3-D model of the nucleus of a PC12 cell The translucent surface and small...

Ngày tải lên: 11/08/2014, 00:22

... Page of 13 Fabrication of AuNPs AuNPs were prepared by using the reduction of chloroauric acid (H[AuCl4]) solution [16,17] Different diameters of AuNPs were made by using different ratios of chloroauric ... different sizes of AuNPs in an array of 500-nm-holes The scattering of AuNPs in the hole can be used to mimic the scattering of AuNP aggregate in the vesicle The preparation of the samples were ... the radius of the nanoparticle, l is the incident wavelength, n is the refractive index of environmental medium and εr and εi are the real and imaginary parts of the dielectric constant of the nanoparticle,...

Ngày tải lên: 11/08/2014, 00:22

... technology of an offline 3D TEE reconstruction technique helped us to identify that the mass was trapped between the leads of the PM and defined the origin of the thrombus Although 3D TEE is a time- consuming ... three-dimensional offline reconstruction of the two-dimensional transesophageal data Three-dimensional offline reconstruction of the two-dimensional transesophageal data helped us to identify the origin of ... for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal Maagh et al Journal of Medical Case...

Ngày tải lên: 11/08/2014, 02:22

... incomplete analysis, including use of only one examiner, small sample sizes, and measurement of only one region of the body, or a limited number of degrees of freedom of postural displacements [10-16] ... index of reliability Therefore, SEMs were calculated for both ICC methods, of which the conservative method has the lowest ICCs and highest SEMs If SD represents the standard deviation of the ... to each of the measurements of the other examiners, then there were twelve pairs of differences for each variable The more conservative MADBO error analysis averaged all of the 12 pairs of differences...

Ngày tải lên: 13/08/2014, 14:20

... complete loss of fibers at depths >10 µm from the surface, spatially heterogeneous gain of axons, irregularly shaped synaptic boutons, and formation of axonal plexuses composed of nested loops of variable ... attributes of the heart muscle The goals of my thesis work were twofold First, development and validation of an assay for imaging sympathetic nerves in the living mouse heart, and, second, use of this ... microscopy of the living, Langendorff-perfused mouse heart Computerassisted image processing algorithms were used for three-dimensional rendering of fluorescent nerves and generation of 3D wiring...

Ngày tải lên: 24/08/2014, 12:57

... concrete design Time History Analysis Analysis Options Graphic displays of nodal and element time history records • Functions vs time or function vs function displays • SAP2000 • Generation of response ... over element length to model beams of variable sections Rigid End Offsets Frame Elements Rigid End connections to model large joints • SAP2000 • Automated end offset evaluation and assignment ... degrees of freedom) • Base isolator with biaxial plasticity behavior • Base isolator with friction and/or pendulum behavior • SAP2000 • AVI file option for creating real time displays of nonlinear...

Ngày tải lên: 11/06/2014, 03:16

... from you iii Table of Contents Acknowledgements ii Table of Contents iv Summary viii List of Tables x List of Figures xi List of Symbols xviii Introduction 1.1 Background of Water Waves Modeling ... the incident wave H0 initial height of the hump (1) Hm Hankel function of the first kind of order m Jm Bessel function of the first kind of order m kn wave number of the n-th mode L wave length Ls ... point of view, most of the fluid problems can be described by the Navier-Stokes equation However, because of the constraint of the current computer power, it is impossible to resolve all of the...

Ngày tải lên: 13/09/2015, 20:56

... i Table of contents ii Summary iv List of tables .vii List of figures viii List of abbreviations xviii List of symbols ... Summary A vast body of literature shows that the electrospinning technique offers unique advantages in the production of tissue engineering scaffolds compared to other methods in terms of simplicity, ... electrospinning of PCL/Col fibers and electrospraying of HeprasilTM hydrogel (A) Collagen was localized on the surface of these fibers as shown by immunofluorescence staining (B) A higher resolution image of...

Ngày tải lên: 14/09/2015, 08:24

... at a magnification of 10X (b) Example of misalignment on right hand side of a wafer Alignment marks of the electroplated underneath layer not coincide with the alignment marks of the upper layer ... (a) level of the fabricated 2SP strings (b) level of the manufactured P strings (c) level of the P strings (d) a bird’s eye view of the strings…………….……………………………………….……… 71 3.22 (a) Layer of the ... extended to other forms of mass fabrication like plastic molding Below is a brief description of each chapter found in this thesis: Chapter gives an overview of the basic definitions of permittivity,...

Ngày tải lên: 06/10/2015, 20:42

... number of research papers of some International Journals Dr Das is currently acting as the honorary member of editorial board of Indian Journal of Science and Technology and as Referee of AMSE Journal, ... 21.821 -6.4604 21.806 -6.4648 4.4 Rate of heat transfer The rate of heat transfer at the wall i.e the heat flux in terms of Nusselt number Nu for different values of α and Kp are entered in Table The ... present the effect of suction parameter α and the Reynolds number Re respectively on the temperature of the flow field A careful observation of the above figures shows that the effect of growing suction...

Ngày tải lên: 05/09/2013, 14:58