materials growing conditions and sampling

... WT leaves and leaves of the pdx1 mutant before and after high light stress (LL and HL, respectively) C) Lipid hydroperoxide level (HOTE) in leaves of control and high light-stressed WT and pdx1 ... antenna system (monomeric Lhcb and trimeric LHCII; B2 and B3 bands in Fig 10A, respectively) relative to the PSII reaction center (B5 band) The PSI-LHCI supercomplex (B6 band) was also reduced during ... (data not shown) C and D) SDS-PAGE separation of the B2, B3 and B6 bands using two different buffer systems: tricine (C) and urea (D) See ref [87] for identification of the bands BBY = PSII-enriched...

Ngày tải lên: 12/08/2014, 03:21

... (retinol), D (cholecalciferol) and E (α-tocopherol) in patients with CAD MATERIALS AND METHODS The study population included 30 patients having angiographically demonstrated CAD and 20 patients having ... and serum cholesterol levels were not significantly different in patients with and without CAD (Table 1) Comparisons of the levels of the vitamins (retinol, tocopherol and cholecalciferol), and ... levels and mortality Arch Intern Med 2002; 162: 2443-2449 31 Rock E, Winklhofer-Roob BM, Ribalta J, et al Vitamin A, vitamin E and carotenoid status and metabolism during ageing: functional and...

Ngày tải lên: 25/10/2012, 10:51

... quantities for normal metabolism • Humans cannot synthesize most vitamins and therefore need to obtain them from food and supplements • Insufficient levels of vitamins result in deficiency diseases ... Folate: Enzyme extraction with α-amylase, protease and γ-glutamyl hydrolase(conjugase) – Vitamins A, E, or D: Organic solvent extraction, saponification, and re-extraction with organic solvents For ... ethanolic KOH, vitamin A (retinol) is extracted into organic solvent and then concentrated Vitamin A isomers – all-transretinol and 13-cis-retinol – levels are determined by HPLC on a silica column...

Ngày tải lên: 08/03/2014, 15:35

... in the study and takes responsibility for the integrity of the data and the accuracy of the data analyses Study concept and design: CA and OB Acquisition of data: CA and OB Analysis and interpretation ... supplementation and risk of fractures N Engl J Med 2006, 354:669-683 Law M, Withers H, Morris J, Anderson F: Vitamin D supplementation and the prevention of fractures and falls: results of a randomized ... stroke: a randomized controlled trial Cerebrovasc Dis 2005, 20:187-192 53 Ceglia L: Vitamin D and skeletal muscle tissue and function Mol Aspects Med 2008, 29:407-414 54 de Boland AR, Boland RL:...

Ngày tải lên: 19/06/2014, 08:20

... Radiographs of the hands, wrists, and distal part of the feet were obtained at baseline and after 12 and 24 months Forty joints in the hands and feet were scored (in the hands, proximal interphalangeal ... markers of bone and cartilage turnover, the correlations between the markers and bone mass and joint damage, and the associations between changes in biochemical markers and changes in BMD and joint ... Diagnostica, Espoo, Finland) The detection limit of the test was 0.5 µg/l and the intra-assay and interassay coefficients of variation were

Ngày tải lên: 09/08/2014, 01:24

... cytosine-phosphate-guanosine Tisman and Garcia Journal of Medical Case Reports 2011, 5:413 http://www.jmedicalcasereports.com/content/5/1/413 (CpG) islands and cancer initiation, promotion, and progression is ... groups), support methyl group generation and folate metabolism SAM delivers the methyl groups responsible for promoter CpG islands and global DNA cytosine Tisman and Garcia Journal of Medical Case ... factor and 500 μg of vitamin B12, as well as 400 μg of FA, and 400 μg of L-5-methyltetrahydrofolate (for a total of 800 μg of mixed FAs) On 11 February 2010, his PSA level reached 21.3 ng/mL, and...

Ngày tải lên: 10/08/2014, 23:20

... Eicosapentaenoic acid and arachidonic acid: collaboration and not antagonism is the key to biological understanding Prostaglandins Leukot Essent Fatty Acids 2002, 66(1):83-90 Simopoulos A, Cleland L: Omega-6/Omega-3 ... the Scandinavian Women's Lifestyle and Health Cohort [19,20] The women were asked to fill in a paper questionnaire, including a food frequency questionnaire (FFQ), and levels of fish, PUFAs and ... the questionnaires and were enrolled in the study In 2002/2003 a follow-up study was initiated, and women who had responded to the 1991/1992 questionnaire and who were alive and living in Sweden...

Ngày tải lên: 11/08/2014, 16:22

... RM: Vitamin D in overweight/obese women and its relationship with dietetic and anthropometric variables Obesity 2009, 17:778-782 McGill A-T, Stewart JM, Lithander FE, Strik CM, Poppitt SD: Relationships ... Poppitt SD: Relationships of low serum vitamin D3 with anthropometry and markers of the metabolic syndrome and diabetes in overweight and obesity Nutr J 2008, 7:4 Louie JK, Acosta M, Winter K, Jean ... viral PAMP- and virus-induced pulmonary innate immune and remodeling responses in mice J Clin Invest 2008, 118:2771-2784 doi:10.1186/cc8894 Cite this article as: Krstić G: Th17 mediators and vitamin...

Ngày tải lên: 13/08/2014, 20:21

Ngày tải lên: 13/08/2014, 20:22

... II Vitamin B3: Nicotinic acid and Vitamin B3the nicotinamide coenzymes : NAD+ and NADP+ III Vitamin B2: Riboflavin and the Flavin Coenzymes FAD, FMN IV Summary and Question I INTRODUCTION What ... B2 IV QUESTIONS AND CONCLUSION  How many electrons NAD and FAD can carry? Answer: NADH can transfer electrons and proton while FAD can accept or donate electrons or at a time and also or protons ... carrying hydrogens and their electrons during metabolic reactions II.Nictotinic acid Nicotinamide adenine dinucleotide (phosphate) NAD NADP Reaction of NAD Differences between NAD/NADH and NADP/NADPH...

Ngày tải lên: 07/11/2014, 15:05

Ngày tải lên: 20/07/2015, 23:47

... constant support, care and understanding all time during my candidature I am grateful to my senior, Cao Na, for her extended help and advice and who has imparted her knowledge and expertise in the ... CELLULAR UPTAKE AND CELL CYTOTOXICITY OF TPGS-DOX AND TPGS-DOX-FOL CONJUGATES…….91 4.1 Introduction………………………………………………………………………… 91 4.2 Materials and Methods……………………………………………………………….91 4.2.1 Materials ……………………………………………………………… ... discussions and timely help I would also like to thank the lab officers, Mrs Tan Mei Yee Dinah, Mr Boey Kok Hong, Ms Chai Keng and the other lab officers from Chemical and Biomolecular Engineering and...

Ngày tải lên: 16/10/2015, 15:36

... Uses: to tone, balance, and strengthen liver function and for hepatitis and liver inflammation/pain for high cholesterol for skin care and skin anti-aging (for its antioxidant and ultraviolet ray ... to 455 nm and 480 to 484 nm Attributed Medicinal Properties ANNATTO HERBAL PROPERTIES AND ACTIONS Main Actions Other Actions Standard Dosage · reduces acid · reduces inflammation Seed and Leaves ... 0.3% to 0.9% essential oil, 3% fixed oil, 4.5% to 5.5% pigments, and 13% to 16% protein, as well as alpha- and beta-carotenoids and other constituents I- Introduction Brick red, triangular in...

Ngày tải lên: 23/10/2013, 02:15

... spectrum (Fig 6A), the correlation between 3-CH and 4-CH2 (2.53 p.p.m and 2.19 p.p.m.), and the correlation between 3-CH and 2-CH2 (1.97 p.p.m and 1.52 p.p.m.), are clearly intact In the TOCSY ... collection and interpretation of the NMR data Their spectra were recorded in CDCl3 and COSY spectra lacked fine detail HSQC and expanded COSY were not provided by Guryev et al [2] and, without ... from two independent samples shows that C23, and not C22, is hydroxylated Minor products and ⁄ or intermediates were identified by purifying them and determining the substrates from which they...

Ngày tải lên: 18/02/2014, 17:20

... expands the boundaries of signalling mediators, and it is therefore not unlikely that Sky and Mer could be involved in Table Affinities of Gas6 and protein S for Axl, Sky and Mer RTKs within and ... Hafizi and B Dahlback ¨ The Gas6–Axl system A Fig (A) Crystal complex of Gas6 LG domains with the Ig domains of Axl Gas6 LG domains are in cyan (N-terminal segment and LG1) and green (LG2), and ... ligand-independent homophilic or heterophilic interaction between two Axl ⁄ Sky monomers (2) Ligand-induced dimerization of Axl monomers from two : (ligand–receptor) complexes to one : (2 · ligand–receptor)...

Ngày tải lên: 19/02/2014, 05:20

... liver and lung samples were taken and processed immediately Retinol and retinyl palmitate were measured in serum and tissues following the method described by Barua and Olson [16] Differential display ... under denaturing conditions in a Genomix LR DNA sequencer (Genomix, Beckman) Gels were dried and exposed to produce an autoradiograph Bands of interest were excised from the gel, and the gel slides ... in a 1% agarose gel under denaturing conditions RNAs were then blotted and fixed to Nytran membranes (Schleicher and Schuell, Keene, NH) Prehybridization and hybridization were performed as described...

Ngày tải lên: 08/03/2014, 08:20

... analyzed free vitamin E and γ-oryzanol components in RG and FFRB The profiles of free vitamin E and γ-oryzanol in RG and FFRB differ significantly The levels of free vitamin E and γ-oryzanol are the ... germ and bran has not been well-studied The aim of the current study was to quantitatively analyze components of vitamin E and γ-oryzanol in rice germ and chemically distinguish rice germ and ... retention time and both positive- and negative-ion spectra from electrospray ionization mass spectrometry (ESI-MS) The levels of γ-oryzanol and vitamin E components in rice germ and bran were...

Ngày tải lên: 15/03/2014, 15:33

... sheath and auxiliary gas, which were set to 20 and units, respectively The source current was set to lA and the capillary voltage was 49 V Vaporizer and capillary temperatures were 225 and 175 ... (F); lutein (G) and 3,3¢-dihydoxyisorenieratene (H) The substrates were cleaved at the C13-C14 and the C15-C15¢ double bonds Preferred and less targeted sites are shaded in dark and light gray, ... Finland), in accordance with the manufacturer’s instructions The obtained product was purified using GFXÔ PCR DNA and Gel Band Purification Kit (Amersham Biosciences, Piscataway, NJ, USA) and cloned...

Ngày tải lên: 15/03/2014, 23:20

... was clearly different from glutaredox12 in and PDI SDS PAGE analysis gave a single band at 31 kDa and Km values of 0.25 and 2.8 mm were reported for DHA and GSH, respectively The enzyme had a specic ... scurvy, in man and in animals unable to synthesize the vitamin Important progress has been made recently in our understanding of the synthesis and the recycling of vitamin C, and a novel pathway ... [37] and now referred to as aldo-keto reductase 1A1 (AKR1A1) for the human enzyme [38] Km values of 0.33 and 0.69 mm were obtained for d-glucuronate and d-glucuronolactone, respectively, and these...

Ngày tải lên: 16/03/2014, 12:20

... We have cloned and expressed the Conus carboxylase in order to prove that this gene encodes a vitamin K-dependent carboxylase, and identified structural and functional similarities and differences ... oligonucleotide primers, serum-free adapted Sf21 cells and Sf 900-II SFM medium were obtained from Gibco–BRL Life Tech- nologies (Grand Island, NY, USA) RACE kits and Advantage cDNA polymerase mix were from ... phenylmethanesulfonyl fluoride and 20% (v/v) glycerol and sonicated COS7 cells (5 · 106 cells) were washed with NaCl/Pi, trypsinized and collected in NaCl/Pi (pH 7.2) containing 20% glycerol and · PIC Cells...

Ngày tải lên: 17/03/2014, 10:20